Abstract:
:We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, activity, or function. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Rigaut G,Shevchenko A,Rutz B,Wilm M,Mann M,Séraphin Bdoi
10.1038/13732subject
Has Abstractpub_date
1999-10-01 00:00:00pages
1030-2issue
10eissn
1087-0156issn
1546-1696journal_volume
17pub_type
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