Abstract:
:To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently formed MACs that segregated accurately and bound CENP-B, CENP-C, and CENP-E. The MACs appear to be about 1-5 Mb in size and contain YAC multimers. Structural analyses suggest that the MACs have not acquired host sequences and were formed by a de novo mechanism. The accurate segregation of the MACs suggests they have potential as vectors for introducing genes into mammals.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Ikeno M,Grimes B,Okazaki T,Nakano M,Saitoh K,Hoshino H,McGill NI,Cooke H,Masumoto Hdoi
10.1038/nbt0598-431subject
Has Abstractpub_date
1998-05-01 00:00:00pages
431-9issue
5eissn
1087-0156issn
1546-1696journal_volume
16pub_type
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