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CRISPR knockout screening outperforms shRNA and CRISPRi in identifying essential genes.

Abstract：

:High-throughput genetic screens have become essential tools for studying a wide variety of biological processes. Here we experimentally compare systems based on clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) or its transcriptionally repressive variant, CRISPR-interference (CRISPRi), with a traditional short hairpin RNA (shRNA)-based system for performing lethality screens. We find that the CRISPR technology performed best, with low noise, minimal off-target effects and consistent activity across reagents.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Evers B,Jastrzebski K,Heijmans JP,Grernrum W,Beijersbergen RL,Bernards R

doi

10.1038/nbt.3536

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

631-3

issue

6

eissn

1087-0156

issn

1546-1696

pii

nbt.3536

journal_volume

34

pub_type

杂志文章

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