Prospects for plant-derived antibacterials.

abstract：:Type I polyketide synthase (PKS) genes consist of modules approximately 3-6 kb long, which encode the structures of 2-carbon units in polyketide products. Alteration or replacement of individual PKS modules can lead to the biosynthesis of 'unnatural' natural products but existing techniques for this are time consuming...

journal_title：Nature biotechnology

authors： Menzella HG,Reid R,Carney JR,Chandran SS,Reisinger SJ,Patel KG,Hopwood DA,Santi DV

更新日期：2005-09-01 00:00:00

abstract：:CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), one can reprogram Cas9 to target different sites in the genome with relative ease, but the on-target activity and off-target effects of individual sgRNAs can vary widely. Here, we use ...

journal_title：Nature biotechnology

authors： Doench JG,Fusi N,Sullender M,Hegde M,Vaimberg EW,Donovan KF,Smith I,Tothova Z,Wilen C,Orchard R,Virgin HW,Listgarten J,Root DE

更新日期：2016-02-01 00:00:00

abstract：:The green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its fluorescent homologs from Anthozoa corals have become invaluable tools for in vivo imaging of cells and tissues. Despite spectral and chromophore diversity, about 100 cloned members of the GFP-like protein family possess common structural...

journal_title：Nature biotechnology

authors： Verkhusha VV,Lukyanov KA

更新日期：2004-03-01 00:00:00

abstract：:Human organ replacement is limited by a donor shortage, problems with tissue compatibility, and rejection. Creation of an organ with autologous tissue would be advantageous. In this study, transplantable urinary bladder neo-organs were reproducibly created in vitro from urothelial and smooth muscle cells grown in cult...

journal_title：Nature biotechnology

authors： Oberpenning F,Meng J,Yoo JJ,Atala A

更新日期：1999-02-01 00:00:00

abstract：:The advent of in vitro DNA amplification has enabled rapid acquisition of genomic information. We present here an analogous technique for protein detection, in which the coordinated and proximal binding of a target protein by two DNA aptamers promotes ligation of oligonucleotides linked to each aptamer affinity probe....

journal_title：Nature biotechnology

authors： Fredriksson S,Gullberg M,Jarvius J,Olsson C,Pietras K,Gústafsdóttir SM,Ostman A,Landegren U

更新日期：2002-05-01 00:00:00

abstract：:A T-cell receptor mimic peptide (TCRpep) consisting of an 8-amino-acid peptide, homologous to the transmembrane region of the T-cell receptor (TCR) alpha chain, blocks T-cell activation after systemic application. When dendritic cells (DCs) were transduced to secrete the TCRpep and injected into mice, evidence of immu...

journal_title：Nature biotechnology

authors： Mahnke K,Qian Y,Knop J,Enk AH

更新日期：2003-08-01 00:00:00

abstract：:Gossypium hirsutum has proven difficult to sequence owing to its complex allotetraploid (AtDt) genome. Here we produce a draft genome using 181-fold paired-end sequences assisted by fivefold BAC-to-BAC sequences and a high-resolution genetic map. In our assembly 88.5% of the 2,173-Mb scaffolds, which cover 89.6%∼96.7%...

journal_title：Nature biotechnology

authors： Li F,Fan G,Lu C,Xiao G,Zou C,Kohel RJ,Ma Z,Shang H,Ma X,Wu J,Liang X,Huang G,Percy RG,Liu K,Yang W,Chen W,Du X,Shi C,Yuan Y,Ye W,Liu X,Zhang X,Liu W,Wei H,Wei S,Huang G,Zhang X,Zhu S,Zhang H,Sun

更新日期：2015-05-01 00:00:00

abstract：:Single-molecule long-read DNA sequencing with biological nanopores is fast and high-throughput but suffers reduced accuracy in homonucleotide stretches. We now combine the CsgG nanopore with the 35-residue N-terminal region of its extracellular interaction partner CsgF to produce a dual-constriction pore with improved...

journal_title：Nature biotechnology

authors： Van der Verren SE,Van Gerven N,Jonckheere W,Hambley R,Singh P,Kilgour J,Jordan M,Wallace EJ,Jayasinghe L,Remaut H

更新日期：2020-12-01 00:00:00

abstract：:Many promising targets for T-cell-based cancer immunotherapies are self-antigens. During thymic selection, T cells bearing T cell receptors (TCRs) with high affinity for self-antigen are eliminated. The affinity of the remaining low-avidity TCRs can be improved to increase their antitumor efficacy, but conventional sa...

journal_title：Nature biotechnology

authors： Schmitt TM,Aggen DH,Ishida-Tsubota K,Ochsenreither S,Kranz DM,Greenberg PD

更新日期：2017-12-01 00:00:00

abstract：:The specificity of biological regulatory mechanisms relies on selective interactions between different proteins in different cell types and in response to different extracellular signals. We describe a bimolecular fluorescence complementation (BiFC) approach for the simultaneous visualization of multiple protein inter...

journal_title：Nature biotechnology

authors： Hu CD,Kerppola TK

更新日期：2003-05-01 00:00:00

abstract：:Most microorganisms from all taxonomic levels are uncultured. Single-cell genomes and metagenomes continue to increase the known diversity of Bacteria and Archaea; however, while 'omics can be used to infer physiological or ecological roles for species in a community, most of these hypothetical roles remain unvalidate...

journal_title：Nature biotechnology

authors： Cross KL,Campbell JH,Balachandran M,Campbell AG,Cooper SJ,Griffen A,Heaton M,Joshi S,Klingeman D,Leys E,Yang Z,Parks JM,Podar M

更新日期：2019-11-01 00:00:00

abstract：:Individual genetic variation affects gene responsiveness to stimuli, often by influencing complex molecular circuits. Here we combine genomic and intermediate-scale transcriptional profiling with computational methods to identify variants that affect the responsiveness of genes to stimuli (responsiveness quantitative ...

journal_title：Nature biotechnology

authors： Gat-Viks I,Chevrier N,Wilentzik R,Eisenhaure T,Raychowdhury R,Steuerman Y,Shalek AK,Hacohen N,Amit I,Regev A

更新日期：2013-04-01 00:00:00

abstract：:Lysosomal storage diseases are treated with human lysosomal enzymes produced in mammalian cells. Such enzyme therapeutics contain relatively low levels of mannose-6-phosphate, which is required to target them to the lysosomes of patient cells. Here we describe a method for increasing mannose-6-phosphate modification o...

journal_title：Nature biotechnology

authors： Tiels P,Baranova E,Piens K,De Visscher C,Pynaert G,Nerinckx W,Stout J,Fudalej F,Hulpiau P,Tännler S,Geysens S,Van Hecke A,Valevska A,Vervecken W,Remaut H,Callewaert N

更新日期：2012-12-01 00:00:00

abstract：:Enzymatic removal of blood group ABO antigens to develop universal red blood cells (RBCs) was a pioneering vision originally proposed more than 25 years ago. Although the feasibility of this approach was demonstrated in clinical trials for group B RBCs, a major obstacle in translating this technology to clinical pract...

journal_title：Nature biotechnology

authors： Liu QP,Sulzenbacher G,Yuan H,Bennett EP,Pietz G,Saunders K,Spence J,Nudelman E,Levery SB,White T,Neveu JM,Lane WS,Bourne Y,Olsson ML,Henrissat B,Clausen H

更新日期：2007-04-01 00:00:00

abstract：:As the cost of human genome sequencing plunges and large-scale genome-phenotype studies become possible, society should do more to reward those individuals who choose to disclose their data, despite the risks. ...

journal_title：Nature biotechnology

authors：

更新日期：2009-09-01 00:00:00

abstract：:Thermostable enzymes combine catalytic specificity with the toughness required to withstand industrial reaction conditions. Stabilized enzymes also provide robust starting points for evolutionary improvement of other protein properties. We recently created a library of at least 2,300 new active chimeras of the biotech...

journal_title：Nature biotechnology

authors： Li Y,Drummond DA,Sawayama AM,Snow CD,Bloom JD,Arnold FH

更新日期：2007-09-01 00:00:00

abstract：:Noncoding RNAs (ncRNAs) are emerging as key molecules in human cancer, with the potential to serve as novel markers of disease and to reveal uncharacterized aspects of tumor biology. Here we discover 121 unannotated prostate cancer-associated ncRNA transcripts (PCATs) by ab initio assembly of high-throughput sequencin...

journal_title：Nature biotechnology

authors： Prensner JR,Iyer MK,Balbin OA,Dhanasekaran SM,Cao Q,Brenner JC,Laxman B,Asangani IA,Grasso CS,Kominsky HD,Cao X,Jing X,Wang X,Siddiqui J,Wei JT,Robinson D,Iyer HK,Palanisamy N,Maher CA,Chinnaiyan AM

更新日期：2011-07-31 00:00:00

abstract：:Development of a cell therapy for diabetes would be greatly aided by a renewable supply of human beta-cells. Here we show that pancreatic endoderm derived from human embryonic stem (hES) cells efficiently generates glucose-responsive endocrine cells after implantation into mice. Upon glucose stimulation of the implant...

journal_title：Nature biotechnology

authors： Kroon E,Martinson LA,Kadoya K,Bang AG,Kelly OG,Eliazer S,Young H,Richardson M,Smart NG,Cunningham J,Agulnick AD,D'Amour KA,Carpenter MK,Baetge EE

更新日期：2008-04-01 00:00:00

abstract：:De novo assembly of a human genome using nanopore long-read sequences has been reported, but it used more than 150,000 CPU hours and weeks of wall-clock time. To enable rapid human genome assembly, we present Shasta, a de novo long-read assembler, and polishing algorithms named MarginPolish and HELEN. Using a single P...

journal_title：Nature biotechnology

authors： Shafin K,Pesout T,Lorig-Roach R,Haukness M,Olsen HE,Bosworth C,Armstrong J,Tigyi K,Maurer N,Koren S,Sedlazeck FJ,Marschall T,Mayes S,Costa V,Zook JM,Liu KJ,Kilburn D,Sorensen M,Munson KM,Vollger MR,Monlong J,Gar

更新日期：2020-09-01 00:00:00

abstract：:A wealth of molecular interaction data is available in the literature, ranging from large-scale datasets to a single interaction confirmed by several different techniques. These data are all too often reported either as free text or in tables of variable format, and are often missing key pieces of information essentia...

journal_title：Nature biotechnology

authors： Orchard S,Salwinski L,Kerrien S,Montecchi-Palazzi L,Oesterheld M,Stümpflen V,Ceol A,Chatr-aryamontri A,Armstrong J,Woollard P,Salama JJ,Moore S,Wojcik J,Bader GD,Vidal M,Cusick ME,Gerstein M,Gavin AC,Superti-Furga G,

更新日期：2007-08-01 00:00:00

abstract：:We report a protocol for the rapid identification of DNA-binding proteins. Immobilized DNA probes harboring a specific sequence motif are incubated with cell or nuclear extract. Proteins are analyzed directly off the solid support by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The det...

journal_title：Nature biotechnology

authors： Nordhoff E,Krogsdam AM,Jorgensen HF,Kallipolitis BH,Clark BF,Roepstorff P,Kristiansen K

更新日期：1999-09-01 00:00:00

abstract：:Plant satellite RNAs generally reduce the level of helper virus accumulation and attenuate the disease symptoms induced by the helper virus that they depend upon for replication and packaging. As such, satellite RNAs could be used as biocontrol agents to reduce the level of disease in field crops, either by the applic...

journal_title：Nature biotechnology

authors： Palukaitis P,Roossinck MJ

更新日期：1996-10-01 00:00:00

abstract：:We describe protein interaction quantitation (PIQ), a computational method for modeling the magnitude and shape of genome-wide DNase I hypersensitivity profiles to identify transcription factor (TF) binding sites. Through the use of machine-learning techniques, PIQ identified binding sites for >700 TFs from one DNase ...

journal_title：Nature biotechnology

authors： Sherwood RI,Hashimoto T,O'Donnell CW,Lewis S,Barkal AA,van Hoff JP,Karun V,Jaakkola T,Gifford DK

更新日期：2014-02-01 00:00:00

abstract：:Forster Resonance Energy Transfer has served as a molecular ruler that reports conformational changes and intramolecular distances of single biomolecules. However, such rulers suffer from low and fluctuating signal intensities, limited observation time due to photobleaching, and an upper distance limit of approximatel...

journal_title：Nature biotechnology

authors： Sönnichsen C,Reinhard BM,Liphardt J,Alivisatos AP

更新日期：2005-06-01 00:00:00

abstract：:Elastin-like polypeptides (ELPs) undergo a reversible, inverse phase transition. Below their transition temperature (Tt), ELPs are soluble in water, but when the temperature is raised above Tt, phase transition occurs, leading to aggregation of the polypeptide. We demonstrate a method for purification of soluble fusio...

journal_title：Nature biotechnology

authors： Meyer DE,Chilkoti A

更新日期：1999-11-01 00:00:00

abstract：:By using an engineered, self-assembling, proteinaceous, 2-nm pore equipped with a metal-actuated switch, a technique to reversibly permeabilize the plasma membrane to small molecules (approximately 1000 Da) has been developed. We have demonstrated the dose-dependent permeabilization of fibroblasts by pores designed to...

journal_title：Nature biotechnology

authors： Russo MJ,Bayley H,Toner M

更新日期：1997-03-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Ramsköld D,Luo S,Wang YC,Li R,Deng Q,Faridani OR,Daniels GA,Khrebtukova I,Loring JF,Laurent LC,Schroth GP,Sandberg R

更新日期：2020-03-01 00:00:00

abstract：:How intrinsic gene-regulatory networks interact with a cell's spatial environment to define its identity remains poorly understood. We developed an approach to distinguish between intrinsic and extrinsic effects on global gene expression by integrating analysis of sequencing-based and imaging-based single-cell transcr...

journal_title：Nature biotechnology

authors： Zhu Q,Shah S,Dries R,Cai L,Yuan GC

更新日期：2018-10-29 00:00:00

abstract：:Colorectal cancer (CRC) is a leading cause of death in the developed world, yet facile preclinical models that mimic the natural stages of CRC progression are lacking. Through the orthotopic engraftment of colon organoids we describe a broadly usable immunocompetent CRC model that recapitulates the entire adenoma-aden...

journal_title：Nature biotechnology

authors： O'Rourke KP,Loizou E,Livshits G,Schatoff EM,Baslan T,Manchado E,Simon J,Romesser PB,Leach B,Han T,Pauli C,Beltran H,Rubin MA,Dow LE,Lowe SW

更新日期：2017-06-01 00:00:00

abstract：:In the version of this article initially published, Betalutin was described as directing a radioisotope to CD45-expressing B cells; in fact, it directs it to CD37-expressing B cells. The error has been corrected in the HTML and PDF versions of the article. ...

journal_title：Nature biotechnology

authors： Dolgin E

更新日期：2019-02-01 00:00:00