Abstract:
:In the version of this article initially published, Betalutin was described as directing a radioisotope to CD45-expressing B cells; in fact, it directs it to CD37-expressing B cells. The error has been corrected in the HTML and PDF versions of the article.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Dolgin Edoi
10.1038/s41587-019-0030-5subject
Has Abstractpub_date
2019-02-01 00:00:00pages
193issue
2eissn
1087-0156issn
1546-1696pii
10.1038/s41587-019-0030-5journal_volume
37pub_type
杂志文章,已发布勘误abstract::Fewer than half of all tandem mass spectrometry (MS/MS) spectra acquired in shotgun proteomics experiments are typically matched to a peptide with high confidence. Here we determine the identity of unassigned peptides using an ultra-tolerant Sequest database search that allows peptide matching even with modifications ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3267
更新日期:2015-07-01 00:00:00
abstract::Increasing the in vivo residence times of protein therapeutics could decrease their dosing frequencies. We show that genetic fusion of an unstructured recombinant polypeptide of 864 amino acids, called XTEN, to a peptide or protein provides an apparently generic approach to extend plasma half-life. Allometric scaling ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1588
更新日期:2009-12-01 00:00:00
abstract::Circular RNA transcripts were first identified in the early 1990s but knowledge of these species has remained limited, as their study through traditional methods of RNA analysis has been difficult. Now, novel bioinformatic approaches coupled with biochemical enrichment strategies and deep sequencing have allowed compr...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2890
更新日期:2014-05-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 杂志文章,已发布勘误
doi:10.1038/s41587-020-0510-7
更新日期:2020-05-01 00:00:00
abstract::We have developed a generic procedure to purify proteins expressed at their natural level under native conditions using a novel tandem affinity purification (TAP) tag. The TAP tag allows the rapid purification of complexes from a relatively small number of cells without prior knowledge of the complex composition, acti...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/13732
更新日期:1999-10-01 00:00:00
abstract::RNA-interference (RNAi) is a potent mechanism, conserved from plants to humans for specific silencing of genes, which holds promise for functional genomics and gene-targeted therapies. Here we show that bacteria engineered to produce a short hairpin RNA (shRNA) targeting a mammalian gene induce trans-kingdom RNAi in v...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1211
更新日期:2006-06-01 00:00:00
abstract::Systemic lupus erythematosus (SLE) is a multisystem chronic inflammatory disease of unknown etiology that affects many organs, including the kidney. The presence of multiple autoantibodies and other immunological abnormalities point to basic defects in immunoregulatory controls that normally maintain self-tolerance. T...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/77296
更新日期:2000-07-01 00:00:00
abstract::We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.4052
更新日期:2018-02-01 00:00:00
abstract::DNA charge transport chemistry is found to provide a sensitive method for probing protein-dependent changes in DNA structure and enzymatic reactions. Here we describe the development of an electrochemical assay of protein binding to DNA-modified electrodes based upon the detection of associated perturbations in DNA ba...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0302-282
更新日期:2002-03-01 00:00:00
abstract::The gold standard for clinical diagnosis of bacterial lower respiratory infections (LRIs) is culture, which has poor sensitivity and is too slow to guide early, targeted antimicrobial therapy. Metagenomic sequencing could identify LRI pathogens much faster than culture, but methods are needed to remove the large amoun...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0156-5
更新日期:2019-07-01 00:00:00
abstract::Can weakly active phytochemicals be combined synergistically to produce new antibacterial treatments? ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1206-1504
更新日期:2006-12-01 00:00:00
abstract::In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two alternatively spliced transcripts, dsx-female (AgdsxF) and dsx-male (AgdsxM), that control differentiation of the two sexes. The female transcript, unlike the male, contains an exon (exon 5) whose sequence is highly conserved in all ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.4245
更新日期:2018-12-01 00:00:00
abstract::A human skin allograft injury model in immunodeficient mice, engrafted with human peripheral blood mononuclear cells from a different donor, has been used to test whether reagents that block human T cell CD2 interactions with its principal ligand, LFA-3 (CD58), can inhibit immune reactions in vivo. In this model, huma...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0897-759
更新日期:1997-08-01 00:00:00
abstract::Most microbes in the biosphere remain unculturable. Whole genome shotgun (WGS) sequencing of environmental DNA (metagenomics) can be used to study the genetic and metabolic properties of natural microbial communities. However, in communities of high complexity, metagenomics fails to link specific microbes to specific ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1488
更新日期:2008-09-01 00:00:00
abstract::L-Ascorbic acid (vitamin C) in fruits and vegetables is an essential component of human nutrition. Surprisingly, only limited information is available about the pathway(s) leading to its biosynthesis in plants. Here, we report the isolation and characterization of GalUR, a gene from strawberry that encodes an NADPH-de...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt777
更新日期:2003-02-01 00:00:00
abstract::Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq ena...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-018-0007-9
更新日期:2019-02-01 00:00:00
abstract::Live cell imaging is a powerful method to study protein dynamics at the cell surface, but conventional imaging probes are bulky, or interfere with protein function, or dissociate from proteins after internalization. Here, we report technology for covalent, specific tagging of cellular proteins with chemical probes. Th...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1355
更新日期:2007-12-01 00:00:00
abstract::Cellular function is critically regulated through degradation of substrates by the proteasome. To enable direct analysis of naturally cleaved proteasomal peptides under physiological conditions, we developed mass spectrometry analysis of proteolytic peptides (MAPP), a method for proteasomal footprinting that allows fo...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.4279
更新日期:2018-10-22 00:00:00
abstract::Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) is a powerful method to quickly and accurately determine the masses of peptides. Most genetic analyses, however, begin with PCR amplification of a test sequence to generate DNA, which is more difficult than peptides to analyze by ...
journal_title:Nature biotechnology
pub_type:
doi:10.1038/72013
更新日期:2000-01-01 00:00:00
abstract::Tocotrienols are the primary form of vitamin E in seeds of most monocot plants, including cereals such as rice and wheat. As potent antioxidants, tocotrienols contribute to the nutritive value of cereal grains in human and livestock diets. cDNAs encoding homogentisic acid geranylgeranyl transferase (HGGT), which catal...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt853
更新日期:2003-09-01 00:00:00
abstract::Individual genetic variation affects gene responsiveness to stimuli, often by influencing complex molecular circuits. Here we combine genomic and intermediate-scale transcriptional profiling with computational methods to identify variants that affect the responsiveness of genes to stimuli (responsiveness quantitative ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2519
更新日期:2013-04-01 00:00:00
abstract::Despite developments in targeted gene sequencing and whole-genome analysis techniques, the robust detection of all genetic variation, including structural variants, in and around genes of interest and in an allele-specific manner remains a challenge. Here we present targeted locus amplification (TLA), a strategy to se...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2959
更新日期:2014-10-01 00:00:00
abstract::Although bile duct disorders are well-recognized causes of liver disease, the molecular and cellular events leading to biliary dysfunction are poorly understood. To enable modeling and drug discovery for biliary disease, we describe a protocol that achieves efficient differentiation of biliary epithelial cells (cholan...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3294
更新日期:2015-08-01 00:00:00
abstract::Antifreeze proteins (AFPs) are found in cold-adapted organisms and have the unusual ability to bind to and inhibit the growth of ice crystals. However, the underlying molecular basis of their ice-binding activity is unclear because of the difficulty of studying the AFP-ice interaction directly and the lack of a common...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1224
更新日期:2006-07-01 00:00:00
abstract::Developments in methods for identifying antigens from infectious agents and cancers has provided exciting new opportunities in prevention and treatment through vaccination. In many of these situations, however, traditional immunization techniques do not stimulate protective immunity because they fail to fully mobilize...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/3469
更新日期:1998-11-01 00:00:00
abstract::Centromeric variation has been linked to cancer and infertility, but centromere sequences contain multiple tandem repeats and can only be assembled manually from long error-prone reads. Here we describe the centroFlye algorithm for centromere assembly using long error-prone reads, and apply it to assemble human centro...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0582-4
更新日期:2020-11-01 00:00:00
abstract::Phosphorus (P) is one of the most important nutrients limiting agricultural production worldwide. In acid and alkaline soils, which make up over 70% of the world's arable land, P forms insoluble compounds that are not available for plant use. To reduce P deficiencies and ensure plant productivity, nearly 30 million to...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/74531
更新日期:2000-04-01 00:00:00
abstract::We report the use of an atomic force microscopy (AFM)-based force spectroscopy technique to identify, at the single-molecule level, the components of mixtures of polysaccharides. Previously, we showed that the elasticity of certain types of polysaccharides is governed by force-induced conformational transitions of the...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/85712
更新日期:2001-03-01 00:00:00
abstract::Transcriptional silencing, one of the major impediments to gene therapy in humans, is often accompanied by replication during late S-phase. We report that transcriptional silencing and late replication were prevented by DNA sequences that can initiate DNA replication (replicators). When replicators were included in si...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1202
更新日期:2006-05-01 00:00:00
abstract::Reproducibility in research can be compromised by both biological and technical variation, but most of the focus is on removing the latter. Here we investigate the effects of biological variation in HeLa cell lines using a systems-wide approach. We determine the degree of molecular and phenotypic variability across 14...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0037-y
更新日期:2019-03-01 00:00:00