Abstract:
:An amendment to this paper has been published and can be accessed via a link at the top of the paper.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Eisenstein Mdoi
10.1038/s41587-020-0510-7subject
Has Abstractpub_date
2020-05-01 00:00:00pages
650issue
5eissn
1087-0156issn
1546-1696pii
10.1038/s41587-020-0510-7journal_volume
38pub_type
杂志文章,已发布勘误abstract::The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic approach for a number of diseases. To overcome the difficulty in generating specific CTLs, we established stable artificial antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any patient of a give...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/74455
更新日期:2000-04-01 00:00:00
abstract::Despite recent accomplishments, significant challenges remain before cloning can become a routine part of transgenic animal production. ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/80231
更新日期:2000-10-01 00:00:00
abstract::Calcium (Ca(2+)) signals that are precisely modulated in space and time mediate a myriad of cellular processes, including contraction, excitation, growth, differentiation and apoptosis. However, study of Ca(2+) responses has been hampered by technological limitations of existing Ca(2+)-modulating tools. Here we presen...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3350
更新日期:2015-10-01 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Nature biotechnology
pub_type: 已发布勘误
doi:10.1038/s41587-020-0578-0
更新日期:2020-08-01 00:00:00
abstract::The clinical translation of cationic α-helical antimicrobial peptides (AMPs) has been hindered by structural instability, proteolytic degradation and in vivo toxicity from nonspecific membrane lysis. Although analyses of hydrophobic content and charge distribution have informed the design of synthetic AMPs with increa...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0222-z
更新日期:2019-10-01 00:00:00
abstract::To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently form...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0598-431
更新日期:1998-05-01 00:00:00
abstract::We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2052
更新日期:2011-11-27 00:00:00
abstract::Protein interaction networks and protein compartmentalization underlie all signaling and regulatory processes in cells. Enzyme-catalyzed proximity labeling (PL) has emerged as a new approach to study the spatial and interaction characteristics of proteins in living cells. However, current PL methods require over 18 h ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.4201
更新日期:2018-10-01 00:00:00
abstract::The side chain of the antifungal antibiotic ansatrienin A from Streptomyces collinus contains a cyclohexanecarboxylic acid (CHC)-derived moiety. This moiety is also observed in trace amounts of omega-cyclohexyl fatty acids (typically less than 1% of total fatty acids) produced by S. collinus. Coenzyme A-activated CHC ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/79479
更新日期:2000-09-01 00:00:00
abstract::We developed a modified flagellar type III secretion apparatus to secrete heterologous polypeptides into the growth medium of Escherichia coli. The secretion was facilitated by fusing the 173-bp untranslated DNA fragment upstream of the gene fliC (encoding flagellin) as well as a transcriptional terminator from fliC, ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1077
更新日期:2005-04-01 00:00:00
abstract::Identification of the peptides recognized by individual T cells is important for understanding and treating immune-related diseases. Current cytometry-based approaches are limited to the simultaneous screening of 10-100 distinct T-cell specificities in one sample. Here we use peptide-major histocompatibility complex (...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3662
更新日期:2016-10-01 00:00:00
abstract::The recently decided eBay and MedImmune cases interject both reassurance and uncertainty into the present patent licensing landscape, affecting the strategic decisions to be considered during negotiations. ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0208-183
更新日期:2008-02-01 00:00:00
abstract::Therapeutic ex vivo T-cell expansion is limited by low rates and T-cell products of limited functionality. Here we describe a system that mimics natural antigen-presenting cells (APCs) and consists of a fluid lipid bilayer supported by mesoporous silica micro-rods. The lipid bilayer presents membrane-bound cues for T-...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.4047
更新日期:2018-02-01 00:00:00
abstract::The scale and capabilities of single-cell RNA-sequencing methods have expanded rapidly in recent years, enabling major discoveries and large-scale cell mapping efforts. However, these methods have not been systematically and comprehensively benchmarked. Here, we directly compare seven methods for single-cell and/or si...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0465-8
更新日期:2020-06-01 00:00:00
abstract::Debates on patenting DNA must evolve to reflect the global decline in filings and regional disparities in patenting activity. ...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt0207-185
更新日期:2007-02-01 00:00:00
abstract::Despite advances in DNA sequencing technology, assembly of complex genomes remains a major challenge, particularly for genomes sequenced using short reads, which yield highly fragmented assemblies. Here we show that genome-wide in vivo chromatin interaction frequency data, which are measurable with chromosome conforma...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2768
更新日期:2013-12-01 00:00:00
abstract::The tubular gland of the chicken oviduct is an attractive system for protein expression as large quantities of proteins are deposited in the egg, the production of eggs is easily scalable and good manufacturing practices for therapeutics from eggs have been established. Here we examined the ability of upstream and dow...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1132
更新日期:2005-09-01 00:00:00
abstract::We describe a novel approach to design a set of primers selective for large groups of genes. This method is based on the distribution frequency of all nucleotide combinations (octa- to decanucleotides), and the combined ability of primer pairs, based on these oligonucleotides, to detect genes. By analyzing 1000 human ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0796-857
更新日期:1996-07-01 00:00:00
abstract::Isoflavones have drawn much attention because of their benefits to human health. These compounds, which are produced almost exclusively in legumes, have natural roles in plant defense and root nodulation. Isoflavone synthase catalyzes the first committed step of isoflavone biosynthesis, a branch of the phenylpropanoid...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/72671
更新日期:2000-02-01 00:00:00
abstract::The ability to detect single protein molecules in blood could accelerate the discovery and use of more sensitive diagnostic biomarkers. To detect low-abundance proteins in blood, we captured them on microscopic beads decorated with specific antibodies and then labeled the immunocomplexes (one or zero labeled target pr...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1641
更新日期:2010-06-01 00:00:00
abstract::Gossypium hirsutum has proven difficult to sequence owing to its complex allotetraploid (AtDt) genome. Here we produce a draft genome using 181-fold paired-end sequences assisted by fivefold BAC-to-BAC sequences and a high-resolution genetic map. In our assembly 88.5% of the 2,173-Mb scaffolds, which cover 89.6%∼96.7%...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3208
更新日期:2015-05-01 00:00:00
abstract::Salicylic acid (SA), produced by plants as a signal in defense against pathogens, induces metabolic heating mediated by alternative respiration in flowers of thermogenic plants, and, when exogenously applied, increases leaf temperature in nonthermogenic plants. We have postulated that the latter phenomenon would be de...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/11765
更新日期:1999-08-01 00:00:00
abstract::Multiple sequence alignments (MSAs) are used for structural1,2 and evolutionary predictions1,2, but the complexity of aligning large datasets requires the use of approximate solutions3, including the progressive algorithm4. Progressive MSA methods start by aligning the most similar sequences and subsequently incorpora...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0333-6
更新日期:2019-12-01 00:00:00
abstract::Organophosphorus hydrolase (OPH) was displayed and anchored onto the surface of Escherichia coli using an Lpp-OmpA fusion system. Production of the fusion proteins in membrane fractions was verified by immunoblotting with OmpA antisera. Inclusion of the organophosphorus hydrolase signal sequence was necessary for achi...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1097-984
更新日期:1997-10-01 00:00:00
abstract::Can weakly active phytochemicals be combined synergistically to produce new antibacterial treatments? ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1206-1504
更新日期:2006-12-01 00:00:00
abstract::For the past five decades, the need for new antibiotics has been met largely by semisynthetic tailoring of natural product scaffolds discovered in the middle of the 20(th) century. More recently, however, advances in technology have sparked a resurgence in the discovery of natural product antibiotics from bacterial so...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt1266
更新日期:2006-12-01 00:00:00
abstract::Dual-color fluorescence cross-correlation spectroscopy (FCCS) is a promising technique for quantifying protein-protein interactions. In this technique, two different fluorescent labels are excited and detected simultaneously within a common measurement volume. Difficulties in aligning two laser lines and emission cros...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1207
更新日期:2006-05-01 00:00:00
abstract::RNA interference (RNAi) induced by short interfering RNA (siRNA) allows for discovery research and large-scale screening; however, owing to their size and anionic charge, siRNAs do not readily enter cells. Current approaches do not deliver siRNAs into a high percentage of primary cells without cytotoxicity. Here we re...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1541
更新日期:2009-06-01 00:00:00
abstract::Paralleling the diversity of genetic and protein activities, pathologic human tissues also exhibit diverse radiographic features. Here we show that dynamic imaging traits in non-invasive computed tomography (CT) systematically correlate with the global gene expression programs of primary human liver cancer. Combinatio...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1306
更新日期:2007-06-01 00:00:00
abstract::Transcriptomic analyses have revealed an unexpected complexity to the human transcriptome, whose breadth and depth exceeds current RNA sequencing capability. Using tiling arrays to target and sequence select portions of the transcriptome, we identify and characterize unannotated transcripts whose rare or transient exp...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2024
更新日期:2011-11-13 00:00:00