Redirecting lipoic acid ligase for cell surface protein labeling with small-molecule probes.

abstract：:We report a protocol for the rapid identification of DNA-binding proteins. Immobilized DNA probes harboring a specific sequence motif are incubated with cell or nuclear extract. Proteins are analyzed directly off the solid support by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The det...

journal_title：Nature biotechnology

authors： Nordhoff E,Krogsdam AM,Jorgensen HF,Kallipolitis BH,Clark BF,Roepstorff P,Kristiansen K

更新日期：1999-09-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Qu L,Yi Z,Zhu S,Wang C,Cao Z,Zhou Z,Yuan P,Yu Y,Tian F,Liu Z,Bao Y,Zhao Y,Wei W

更新日期：2019-11-01 00:00:00

abstract：:Multiple sequence alignments (MSAs) are used for structural1,2 and evolutionary predictions1,2, but the complexity of aligning large datasets requires the use of approximate solutions3, including the progressive algorithm4. Progressive MSA methods start by aligning the most similar sequences and subsequently incorpora...

journal_title：Nature biotechnology

authors： Garriga E,Di Tommaso P,Magis C,Erb I,Mansouri L,Baltzis A,Laayouni H,Kondrashov F,Floden E,Notredame C

更新日期：2019-12-01 00:00:00

abstract：:An approach to the systematic identification and quantification of the proteins contained in the microsomal fraction of cells is described. It consists of three steps: (1) preparation of microsomal fractions from cells or tissues representing different states; (2) covalent tagging of the proteins with isotope-coded af...

journal_title：Nature biotechnology

authors： Han DK,Eng J,Zhou H,Aebersold R

更新日期：2001-10-01 00:00:00

abstract：:After nearly 40 years of development, oligonucleotide therapeutics are nearing meaningful clinical productivity. One of the key advantages of oligonucleotide drugs is that their delivery and potency are derived primarily from the chemical structure of the oligonucleotide whereas their target is defined by the base seq...

journal_title：Nature biotechnology

authors： Khvorova A,Watts JK

更新日期：2017-03-01 00:00:00

abstract：:High-density short oligonucleotide microarrays have become a widely used tool for measuring gene expression on a large scale. However, details of the mechanism of binding on microarrays remain unclear. Short oligonucleotide probes currently synthesized on microarrays are often ineffective as a result of limited sequen...

journal_title：Nature biotechnology

authors： Zhang L,Miles MF,Aldape KD

更新日期：2003-07-01 00:00:00

abstract：:A major shortcoming to the use of adeno-associated virus (rAAV) vectors is their limited packaging size. To overcome this hurdle, we split an expression cassette and cloned it into two separate vectors. The vectors contained either a nuclear localizing Escherichia coli lacZ transgene (nlslacZ) with a splice acceptor, ...

journal_title：Nature biotechnology

authors： Nakai H,Storm TA,Kay MA

更新日期：2000-05-01 00:00:00

abstract：:Excessive and prolonged activity of inflammatory monocytes is a hallmark of many diseases with an inflammatory component. In such conditions, precise targeting of these cells could be therapeutically beneficial while sparing many essential functions of the innate immune system, thus limiting unwanted effects. Inflamma...

journal_title：Nature biotechnology

authors： Leuschner F,Dutta P,Gorbatov R,Novobrantseva TI,Donahoe JS,Courties G,Lee KM,Kim JI,Markmann JF,Marinelli B,Panizzi P,Lee WW,Iwamoto Y,Milstein S,Epstein-Barash H,Cantley W,Wong J,Cortez-Retamozo V,Newton A,Love K,

更新日期：2011-10-09 00:00:00

abstract：:A bacteriophage lambda surface expression system, lambda foo, was used for epitope mapping of human galectin-3. We constructed random epitope and peptide libraries and compared their efficiencies in the mapping. The galectin-3 cDNA was randomly digested by DNase 1 to make random epitope libraries. The libraries were s...

journal_title：Nature biotechnology

authors： Kuwabara I,Maruyama H,Mikawa YG,Zuberi RI,Liu FT,Maruyama IN

更新日期：1997-01-01 00:00:00

abstract：:The scale and capabilities of single-cell RNA-sequencing methods have expanded rapidly in recent years, enabling major discoveries and large-scale cell mapping efforts. However, these methods have not been systematically and comprehensively benchmarked. Here, we directly compare seven methods for single-cell and/or si...

journal_title：Nature biotechnology

authors： Ding J,Adiconis X,Simmons SK,Kowalczyk MS,Hession CC,Marjanovic ND,Hughes TK,Wadsworth MH,Burks T,Nguyen LT,Kwon JYH,Barak B,Ge W,Kedaigle AJ,Carroll S,Li S,Hacohen N,Rozenblatt-Rosen O,Shalek AK,Villani AC,Regev

更新日期：2020-06-01 00:00:00

abstract：:Antisense oligonucleotides are designed to specifically hybridize to a target messenger RNA (mRNA) and interfere with the synthesis of the encoded protein. Uniformly modified oligonucleotides containing N3'-P5' phosphoramidate linkages exhibit (NP) extremely high-affinity binding to single-stranded RNA, do not induce ...

journal_title：Nature biotechnology

authors： Faria M,Spiller DG,Dubertret C,Nelson JS,White MR,Scherman D,Hélène C,Giovannangeli C

更新日期：2001-01-01 00:00:00

abstract：:Purging of tumor cells and selection of stem cells are key technologies for enabling stem cell transplantation and stem cell gene therapy. Here we report a strategy for cell selection based on physical properties of the cells. Exposing cells to an external pulsed electric field (PEF) increases the natural potential di...

journal_title：Nature biotechnology

authors： Eppich HM,Foxall R,Gaynor K,Dombkowski D,Miura N,Cheng T,Silva-Arrieta S,Evans RH,Mangano JA,Preffer FI,Scadden DT

更新日期：2000-08-01 00:00:00

abstract：:Isolating antigen-specific T lymphocytes is hampered by the low frequency of the cells and the low affinity between T-cell receptors (TCR) and antigen. We describe the isolation and purification of antigen-specific CD8+ T lymphocytes from mixed T-cell populations. Magnetic beads coated with major histocompatibility co...

journal_title：Nature biotechnology

authors： Luxembourg AT,Borrow P,Teyton L,Brunmark AB,Peterson PA,Jackson MR

更新日期：1998-03-01 00:00:00

abstract：:We used the integrase from phage phiC31 to integrate the human Factor IX (hFIX) gene permanently into specific sites in the mouse genome. A plasmid containing attB and an expression cassette for hFIX was delivered to the livers of mice by using high-pressure tail vein injection. When an integrase expression plasmid wa...

journal_title：Nature biotechnology

authors： Olivares EC,Hollis RP,Chalberg TW,Meuse L,Kay MA,Calos MP

更新日期：2002-11-01 00:00:00

abstract：:Debates on patenting DNA must evolve to reflect the global decline in filings and regional disparities in patenting activity. ...

journal_title：Nature biotechnology

authors： Hopkins MM,Mahdi S,Patel P,Thomas SM

更新日期：2007-02-01 00:00:00

abstract：:Tissue function depends on hierarchical structures extending from single cells ( approximately 10 microm) to functional subunits (100 microm-1 mm) that coordinate organ functions. Conventional cell culture disperses tissues into single cells while neglecting higher-order processes. The application of semiconductor-dri...

journal_title：Nature biotechnology

authors： Khetani SR,Bhatia SN

更新日期：2008-01-01 00:00:00

abstract：:We have developed a platform for cell analysis based on immunomagnetic selection and magnetic alignment of cells in combination with an epi-illumination tracking and detection system. Whole blood was labeled with ferromagnetic nanoparticles and fluorescent probes, and placed in a magnetic field in a chamber. Cells lab...

journal_title：Nature biotechnology

authors： Tibbe AG,de Grooth BG,Greve J,Liberti PA,Dolan GJ,Terstappen LW

更新日期：1999-12-01 00:00:00

abstract：:Multiple intravenous injections of a cDNA library, derived from human melanoma cell lines and expressed using the highly immunogenic vector vesicular stomatitis virus (VSV), cured mice with established melanoma tumors. Successful tumor eradication was associated with the ability of mouse lymphoid cells to mount a tumo...

journal_title：Nature biotechnology

authors： Pulido J,Kottke T,Thompson J,Galivo F,Wongthida P,Diaz RM,Rommelfanger D,Ilett E,Pease L,Pandha H,Harrington K,Selby P,Melcher A,Vile R

更新日期：2012-03-18 00:00:00

abstract：:Despite advances in DNA sequencing technology, assembly of complex genomes remains a major challenge, particularly for genomes sequenced using short reads, which yield highly fragmented assemblies. Here we show that genome-wide in vivo chromatin interaction frequency data, which are measurable with chromosome conforma...

journal_title：Nature biotechnology

authors： Kaplan N,Dekker J

更新日期：2013-12-01 00:00:00

abstract：:We report the use of an atomic force microscopy (AFM)-based force spectroscopy technique to identify, at the single-molecule level, the components of mixtures of polysaccharides. Previously, we showed that the elasticity of certain types of polysaccharides is governed by force-induced conformational transitions of the...

journal_title：Nature biotechnology

authors： Marszalek PE,Li H,Fernandez JM

更新日期：2001-03-01 00:00:00

abstract：:Fluorescence microscopy of living cells enables visualization of the dynamics and interactions of intracellular molecules. However, fluorescence live-cell imaging is limited by photobleaching and phototoxicity induced by the excitation light. Here we describe controlled light-exposure microscopy (CLEM), a simple imagi...

journal_title：Nature biotechnology

authors： Hoebe RA,Van Oven CH,Gadella TW Jr,Dhonukshe PB,Van Noorden CJ,Manders EM

更新日期：2007-02-01 00:00:00

abstract：:Microbially catalyzed reactions, which occur in the natural sulfur cycle, have been integrated in a microbiological process to remove toxic metals from contaminated soils. Bioleaching using sulfuric acid produced by sulfur-oxidizing bacteria was followed by precipitation of the leachate metals as insoluble sulfides by...

journal_title：Nature biotechnology

authors： White C,Sharman AK,Gadd GM

更新日期：1998-06-01 00:00:00

abstract：:The discovery of large multiprotein complexes in cells has increased the demand for improved heterologous protein production techniques to study their molecular structure and function. Here we describe MultiBac, a simple and versatile system for generating recombinant baculovirus DNA to express protein complexes compr...

journal_title：Nature biotechnology

authors： Berger I,Fitzgerald DJ,Richmond TJ

更新日期：2004-12-01 00:00:00

abstract：:The human genome encodes approximately 100,000 different genes, and at least partial sequence information for nearly all will be available soon. Sequence information alone, however, is insufficient for a full understanding of gene function, expression, regulation, and splice-site variation. Because cellular processes ...

journal_title：Nature biotechnology

authors： Lockhart DJ,Dong H,Byrne MC,Follettie MT,Gallo MV,Chee MS,Mittmann M,Wang C,Kobayashi M,Horton H,Brown EL

更新日期：1996-12-01 00:00:00

abstract：:RNA-interference (RNAi) is a potent mechanism, conserved from plants to humans for specific silencing of genes, which holds promise for functional genomics and gene-targeted therapies. Here we show that bacteria engineered to produce a short hairpin RNA (shRNA) targeting a mammalian gene induce trans-kingdom RNAi in v...

journal_title：Nature biotechnology

authors： Xiang S,Fruehauf J,Li CJ

更新日期：2006-06-01 00:00:00

abstract：:Wild relatives of crops are an important source of genetic diversity for agriculture, but their gene repertoire remains largely unexplored. We report the establishment and analysis of a pan-genome of Glycine soja, the wild relative of cultivated soybean Glycine max, by sequencing and de novo assembly of seven phylogen...

journal_title：Nature biotechnology

authors： Li YH,Zhou G,Ma J,Jiang W,Jin LG,Zhang Z,Guo Y,Zhang J,Sui Y,Zheng L,Zhang SS,Zuo Q,Shi XH,Li YF,Zhang WK,Hu Y,Kong G,Hong HL,Tan B,Song J,Liu ZX,Wang Y,Ruan H,Yeung CK,Liu J,Wang H,Zhang LJ,Guan RX

更新日期：2014-10-01 00:00:00

abstract：:Centromeric variation has been linked to cancer and infertility, but centromere sequences contain multiple tandem repeats and can only be assembled manually from long error-prone reads. Here we describe the centroFlye algorithm for centromere assembly using long error-prone reads, and apply it to assemble human centro...

journal_title：Nature biotechnology

authors： Bzikadze AV,Pevzner PA

更新日期：2020-11-01 00:00:00

abstract：:Dietary omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5) are usually derived from marine fish. Although production of both EPA and DHA has been engineered into land plants, including Arabidopsis, Camelina sativa and Brassica juncea, nei...

journal_title：Nature biotechnology

authors： Walsh TA,Bevan SA,Gachotte DJ,Larsen CM,Moskal WA,Merlo PA,Sidorenko LV,Hampton RE,Stoltz V,Pareddy D,Anthony GI,Bhaskar PB,Marri PR,Clark LM,Chen W,Adu-Peasah PS,Wensing ST,Zirkle R,Metz JG

更新日期：2016-08-01 00:00:00

abstract：:Whole-genome mRNA quantitation can be used to identify the genes that are most responsive to environmental or genotypic change. By searching for mutually similar DNA elements among the upstream non-coding DNA sequences of these genes, we can identify candidate regulatory motifs and corresponding candidate sets of core...

journal_title：Nature biotechnology

authors： Roth FP,Hughes JD,Estep PW,Church GM

更新日期：1998-10-01 00:00:00

abstract：:Complex interactions among genetic components often result in variable systemic performance in designed multigene systems. Using the bacterial clustered regularly interspaced short palindromic repeat (CRISPR) pathway we develop a synthetic RNA-processing platform, and show that efficient and specific cleavage of precu...

journal_title：Nature biotechnology

authors： Qi L,Haurwitz RE,Shao W,Doudna JA,Arkin AP

更新日期：2012-10-01 00:00:00