Fully mobilizing host defense: building better vaccines.

abstract：:Dietary omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs), docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5) are usually derived from marine fish. Although production of both EPA and DHA has been engineered into land plants, including Arabidopsis, Camelina sativa and Brassica juncea, nei...

journal_title：Nature biotechnology

authors： Walsh TA,Bevan SA,Gachotte DJ,Larsen CM,Moskal WA,Merlo PA,Sidorenko LV,Hampton RE,Stoltz V,Pareddy D,Anthony GI,Bhaskar PB,Marri PR,Clark LM,Chen W,Adu-Peasah PS,Wensing ST,Zirkle R,Metz JG

更新日期：2016-08-01 00:00:00

abstract：:How intrinsic gene-regulatory networks interact with a cell's spatial environment to define its identity remains poorly understood. We developed an approach to distinguish between intrinsic and extrinsic effects on global gene expression by integrating analysis of sequencing-based and imaging-based single-cell transcr...

journal_title：Nature biotechnology

authors： Zhu Q,Shah S,Dries R,Cai L,Yuan GC

更新日期：2018-10-29 00:00:00

abstract：:A protocol was established for the introduction of DNA into embryogenic suspension-derived tissues of cassava via microparticle bombardment, for the selection of genetically transformed cells, and for the regeneration of fully transgenic plants from these cells. The plasmid DNA used for bombardment contained a gene en...

journal_title：Nature biotechnology

authors： Schöpke C,Taylor N,Cárcamo R,Konan NK,Marmey P,Henshaw GG,Beachy RN,Fauquet C

更新日期：1996-06-01 00:00:00

abstract：:We show that selection of drug-resistant bacterial mutants allows the discovery of antibacterial compounds. Mutant strains of a soil-isolated Streptomyces species that does not produce antibacterials synthesize a previously unknown class of antibacterial, which we name piperidamycin. Overall, 6% of non-Streptomyces ac...

journal_title：Nature biotechnology

authors： Hosaka T,Ohnishi-Kameyama M,Muramatsu H,Murakami K,Tsurumi Y,Kodani S,Yoshida M,Fujie A,Ochi K

更新日期：2009-05-01 00:00:00

abstract：:A bacteriophage lambda surface expression system, lambda foo, was used for epitope mapping of human galectin-3. We constructed random epitope and peptide libraries and compared their efficiencies in the mapping. The galectin-3 cDNA was randomly digested by DNase 1 to make random epitope libraries. The libraries were s...

journal_title：Nature biotechnology

authors： Kuwabara I,Maruyama H,Mikawa YG,Zuberi RI,Liu FT,Maruyama IN

更新日期：1997-01-01 00:00:00

abstract：:DNA charge transport chemistry is found to provide a sensitive method for probing protein-dependent changes in DNA structure and enzymatic reactions. Here we describe the development of an electrochemical assay of protein binding to DNA-modified electrodes based upon the detection of associated perturbations in DNA ba...

journal_title：Nature biotechnology

authors： Boon EM,Salas JE,Barton JK

更新日期：2002-03-01 00:00:00

abstract：:Little is known about the potential of plastids to accomplish post-translational modifications of foreign proteins. In the present study we generated transplastomic tobacco plants that accumulate the outer surface lipoprotein A (OspA)-the basic constituent of the first generation monovalent human vaccine against Lyme ...

journal_title：Nature biotechnology

authors： Glenz K,Bouchon B,Stehle T,Wallich R,Simon MM,Warzecha H

更新日期：2006-01-01 00:00:00

abstract：:As biotechnology advances, the day may soon come for the creation of a self-aware, human-nonhuman chimera. The USPTO has ruled on whether a patent may issue on such an organism, but Congress must still legislate a dividing line between human and non-human patentable subject matter. ...

journal_title：Nature biotechnology

authors： Rabin S

更新日期：2006-05-01 00:00:00

abstract：:Of paramount importance for the development of cell therapies to treat diabetes is the production of sufficient numbers of pancreatic endocrine cells that function similarly to primary islets. We have developed a differentiation process that converts human embryonic stem (hES) cells to endocrine cells capable of synth...

journal_title：Nature biotechnology

authors： D'Amour KA,Bang AG,Eliazer S,Kelly OG,Agulnick AD,Smart NG,Moorman MA,Kroon E,Carpenter MK,Baetge EE

更新日期：2006-11-01 00:00:00

abstract：:Linear, medium-chain (C8-C12) hydrocarbons are important components of fuels as well as commodity and specialty chemicals. As industrial microbes do not contain pathways to produce medium-chain chemicals, approaches such as overexpression of endogenous enzymes or deletion of competing pathways are not available to the...

journal_title：Nature biotechnology

authors： Sarria S,Kruyer NS,Peralta-Yahya P

更新日期：2017-12-01 00:00:00

abstract：:The Human Proteome Organization (HUPO) recently completed the first large-scale collaborative study to characterize the human serum and plasma proteomes. The study was carried out in different locations and used diverse methods and instruments to compare and integrate tandem mass spectrometry (MS/MS) data on aliquots ...

journal_title：Nature biotechnology

authors： States DJ,Omenn GS,Blackwell TW,Fermin D,Eng J,Speicher DW,Hanash SM

更新日期：2006-03-01 00:00:00

abstract：:Lipid production in the industrial microalga Nannochloropsis gaditana exceeds that of model algal species and can be maximized by nutrient starvation in batch culture. However, starvation halts growth, thereby decreasing productivity. Efforts to engineer N. gaditana strains that can accumulate biomass and overproduce ...

journal_title：Nature biotechnology

authors： Ajjawi I,Verruto J,Aqui M,Soriaga LB,Coppersmith J,Kwok K,Peach L,Orchard E,Kalb R,Xu W,Carlson TJ,Francis K,Konigsfeld K,Bartalis J,Schultz A,Lambert W,Schwartz AS,Brown R,Moellering ER

更新日期：2017-07-01 00:00:00

abstract：:We used the integrase from phage phiC31 to integrate the human Factor IX (hFIX) gene permanently into specific sites in the mouse genome. A plasmid containing attB and an expression cassette for hFIX was delivered to the livers of mice by using high-pressure tail vein injection. When an integrase expression plasmid wa...

journal_title：Nature biotechnology

authors： Olivares EC,Hollis RP,Chalberg TW,Meuse L,Kay MA,Calos MP

更新日期：2002-11-01 00:00:00

abstract：:We describe a novel sequencing approach that combines non-gel-based signature sequencing with in vitro cloning of millions of templates on separate 5 microm diameter microbeads. After constructing a microbead library of DNA templates by in vitro cloning, we assembled a planar array of a million template-containing mic...

journal_title：Nature biotechnology

authors： Brenner S,Johnson M,Bridgham J,Golda G,Lloyd DH,Johnson D,Luo S,McCurdy S,Foy M,Ewan M,Roth R,George D,Eletr S,Albrecht G,Vermaas E,Williams SR,Moon K,Burcham T,Pallas M,DuBridge RB,Kirchner J,Fearon K,Mao J,

更新日期：2000-06-01 00:00:00

abstract：:A broad-specificity delta 9 desaturase gene was cloned from the cyanobacterium Anacystis nidulans. The enzyme introduces a cis-double bond at the delta 9 position of both 16 and 18 carbon saturated fatty acids linked to many kinds of membrane lipids. The gene was stably introduced into tobacco plants under transcripti...

journal_title：Nature biotechnology

authors： Ishizaki-Nishizawa O,Fujii T,Azuma M,Sekiguchi K,Murata N,Ohtani T,Toguri T

更新日期：1996-08-01 00:00:00

abstract：:Multiple sequence alignments (MSAs) are used for structural1,2 and evolutionary predictions1,2, but the complexity of aligning large datasets requires the use of approximate solutions3, including the progressive algorithm4. Progressive MSA methods start by aligning the most similar sequences and subsequently incorpora...

journal_title：Nature biotechnology

authors： Garriga E,Di Tommaso P,Magis C,Erb I,Mansouri L,Baltzis A,Laayouni H,Kondrashov F,Floden E,Notredame C

更新日期：2019-12-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Mitiouchkina T,Mishin AS,Somermeyer LG,Markina NM,Chepurnyh TV,Guglya EB,Karataeva TA,Palkina KA,Shakhova ES,Fakhranurova LI,Chekova SV,Tsarkova AS,Golubev YV,Negrebetsky VV,Dolgushin SA,Shalaev PV,Shlykov D,Melnik OA,

更新日期：2020-08-01 00:00:00

abstract：:Insertional mutagenesis represents a major hurdle to gene therapy and necessitates sensitive preclinical genotoxicity assays. Cdkn2a-/- mice are susceptible to a broad range of cancer-triggering genetic lesions. We exploited hematopoietic stem cells from these tumor-prone mice to assess the oncogenicity of prototypica...

journal_title：Nature biotechnology

authors： Montini E,Cesana D,Schmidt M,Sanvito F,Ponzoni M,Bartholomae C,Sergi Sergi L,Benedicenti F,Ambrosi A,Di Serio C,Doglioni C,von Kalle C,Naldini L

更新日期：2006-06-01 00:00:00

abstract：:We constructed an immunotoxin, composed of an antibody directed against the high-affinity IgG receptor CD64 and Ricin-A, with the aim of resolving chronic inflammation through elimination of activated macrophages. In vitro, this immunotoxin proved very efficient in inducing apoptosis in activated macrophages, leaving ...

journal_title：Nature biotechnology

authors： Thepen T,van Vuuren AJ,Kiekens RC,Damen CA,Vooijs WC,van De Winkel JG

更新日期：2000-01-01 00:00:00

abstract：:We introduce Sailfish, a computational method for quantifying the abundance of previously annotated RNA isoforms from RNA-seq data. Because Sailfish entirely avoids mapping reads, a time-consuming step in all current methods, it provides quantification estimates much faster than do existing approaches (typically 20 ti...

journal_title：Nature biotechnology

authors： Patro R,Mount SM,Kingsford C

更新日期：2014-05-01 00:00:00

abstract：:Technologies that enable targeted manipulation of epigenetic marks could be used to precisely control cell phenotype or interrogate the relationship between the epigenome and transcriptional control. Here we describe a programmable, CRISPR-Cas9-based acetyltransferase consisting of the nuclease-null dCas9 protein fuse...

journal_title：Nature biotechnology

authors： Hilton IB,D'Ippolito AM,Vockley CM,Thakore PI,Crawford GE,Reddy TE,Gersbach CA

更新日期：2015-05-01 00:00:00

abstract：:Genetic testing for rare heart conditions might someday expand to more common cardiac ailments. Already there are signs testing is dramatically changing how some conditions are treated and doctors' definition of who a patient is. Stephen Strauss reports. ...

journal_title：Nature biotechnology

authors： Strauss S

更新日期：2010-10-01 00:00:00

abstract：:Tocotrienols are the primary form of vitamin E in seeds of most monocot plants, including cereals such as rice and wheat. As potent antioxidants, tocotrienols contribute to the nutritive value of cereal grains in human and livestock diets. cDNAs encoding homogentisic acid geranylgeranyl transferase (HGGT), which catal...

journal_title：Nature biotechnology

authors： Cahoon EB,Hall SE,Ripp KG,Ganzke TS,Hitz WD,Coughlan SJ

更新日期：2003-09-01 00:00:00

abstract：:The targeting scope of Streptococcus pyogenes Cas9 (SpCas9) and its engineered variants is largely restricted to protospacer-adjacent motif (PAM) sequences containing G bases. Here we report the evolution of three new SpCas9 variants that collectively recognize NRNH PAMs (where R is A or G and H is A, C or T) using ph...

journal_title：Nature biotechnology

authors： Miller SM,Wang T,Randolph PB,Arbab M,Shen MW,Huang TP,Matuszek Z,Newby GA,Rees HA,Liu DR

更新日期：2020-04-01 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) that have the potential to elicit new bone in vivo have been used in a tissue-engineering approach for the repair of bone injuries and bone defects. Although it is now possible to generate large amounts of recombinant human (rh) BMPs for medical use, the major challenge remains in th...

journal_title：Nature biotechnology

authors： Saito N,Okada T,Horiuchi H,Murakami N,Takahashi J,Nawata M,Ota H,Nozaki K,Takaoka K

更新日期：2001-04-01 00:00:00

abstract：:The human transcriptome is marked by extensive alternative mRNA splicing and the expression of many closely related genes, which may be difficult to distinguish using standard microarray techniques. Here we describe a sensitive and specific assay for parallel analysis of mRNA isoforms on a fiber-optic microarray platf...

journal_title：Nature biotechnology

authors： Yeakley JM,Fan JB,Doucet D,Luo L,Wickham E,Ye Z,Chee MS,Fu XD

更新日期：2002-04-01 00:00:00

abstract：:The sap-sucking insects (order Hemiptera), including aphids, planthoppers, whiteflies and stink bugs, present one of the greatest challenges for pest management in global agriculture. Insect neurotoxins offer an alternative to chemical insecticides for controlling these pests, but require delivery into the insect hemo...

journal_title：Nature biotechnology

authors： Bonning BC,Pal N,Liu S,Wang Z,Sivakumar S,Dixon PM,King GF,Miller WA

更新日期：2014-01-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Röst HL,Rosenberger G,Navarro P,Gillet L,Miladinović SM,Schubert OT,Wolski W,Collins BC,Malmström J,Malmström L,Aebersold R

更新日期：2020-03-01 00:00:00

abstract：:We present 1,003 reference genomes that were sequenced as part of the Genomic Encyclopedia of Bacteria and Archaea (GEBA) initiative, selected to maximize sequence coverage of phylogenetic space. These genomes double the number of existing type strains and expand their overall phylogenetic diversity by 25%. Comparativ...

journal_title：Nature biotechnology

authors： Mukherjee S,Seshadri R,Varghese NJ,Eloe-Fadrosh EA,Meier-Kolthoff JP,Göker M,Coates RC,Hadjithomas M,Pavlopoulos GA,Paez-Espino D,Yoshikuni Y,Visel A,Whitman WB,Garrity GM,Eisen JA,Hugenholtz P,Pati A,Ivanova NN,Woyke

更新日期：2017-07-01 00:00:00

abstract：:Protein phosphorylation is a key post-translational modification regulating protein function in almost all cellular processes. Although tens of thousands of phosphorylation sites have been identified in human cells, approaches to determine the functional importance of each phosphosite are lacking. Here, we manually cu...

journal_title：Nature biotechnology

authors： Ochoa D,Jarnuczak AF,Viéitez C,Gehre M,Soucheray M,Mateus A,Kleefeldt AA,Hill A,Garcia-Alonso L,Stein F,Krogan NJ,Savitski MM,Swaney DL,Vizcaíno JA,Noh KM,Beltrao P

更新日期：2020-03-01 00:00:00