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Resolution of cutaneous inflammation after local elimination of macrophages.

Abstract：

:We constructed an immunotoxin, composed of an antibody directed against the high-affinity IgG receptor CD64 and Ricin-A, with the aim of resolving chronic inflammation through elimination of activated macrophages. In vitro, this immunotoxin proved very efficient in inducing apoptosis in activated macrophages, leaving resting and low CD64-expressing macrophages unaffected. We examined the activity of our immunotoxin in a sodium lauryl sulfate (SLS)-induced cutaneous inflammation model, using transgenic mice expressing human CD64. Upon intradermal injection of the immunotoxin (IT), cutaneous inflammation resolved in 24 h. This was demonstrated histologically by clearance of all CD64-expressing macrophages, followed by clearance of other inflammatory cells. Clinical parameters associated with inflammation, such as local skin temperature and vasodilation, also decreased.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Thepen T,van Vuuren AJ,Kiekens RC,Damen CA,Vooijs WC,van De Winkel JG

doi

10.1038/71908

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

48-51

issue

1

eissn

1087-0156

issn

1546-1696

journal_volume

18

pub_type

杂志文章

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