Abstract:
:St. Louis wants to become a hub of agricultural biotechnology. All it needs is more start-ups and funds.
journal_name
Nat Biotechnoljournal_title
Nature biotechnologyauthors
Marris Edoi
10.1038/nbt0408-471subject
Has Abstractpub_date
2008-04-01 00:00:00pages
471-2issue
4eissn
1087-0156issn
1546-1696pii
nbt0408-471journal_volume
26pub_type
杂志文章abstract::Efficient delivery of small interfering (si)RNA to specific cell populations in vivo remains a formidable challenge to its successful therapeutic application. We show that siRNA synthetically linked to a CpG oligonucleotide agonist of toll-like receptor (TLR)9 targets and silences genes in TLR9(+) myeloid cells and B ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.1564
更新日期:2009-10-01 00:00:00
abstract::Can weakly active phytochemicals be combined synergistically to produce new antibacterial treatments? ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1206-1504
更新日期:2006-12-01 00:00:00
abstract::We compared bona fide human induced pluripotent stem cells (iPSCs) derived from umbilical cord blood (CB) cells and neonatal keratinocytes (K). As a consequence of both incomplete erasure of tissue-specific methylation and aberrant de novo methylation, CB-iPSCs and K-iPSCs were distinct in genome-wide DNA methylation ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2052
更新日期:2011-11-27 00:00:00
abstract::A broad-specificity delta 9 desaturase gene was cloned from the cyanobacterium Anacystis nidulans. The enzyme introduces a cis-double bond at the delta 9 position of both 16 and 18 carbon saturated fatty acids linked to many kinds of membrane lipids. The gene was stably introduced into tobacco plants under transcripti...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0896-1003
更新日期:1996-08-01 00:00:00
abstract::In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two alternatively spliced transcripts, dsx-female (AgdsxF) and dsx-male (AgdsxM), that control differentiation of the two sexes. The female transcript, unlike the male, contains an exon (exon 5) whose sequence is highly conserved in all ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.4245
更新日期:2018-12-01 00:00:00
abstract::Efficiently generating evidence of clinical utility is a major challenge for ensuring clinical adoption of valuable diagnostics. A new approach to reimbursement in the United States offers a balance between evidence and incentives for molecular diagnostic tests. ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1110-1157
更新日期:2010-11-01 00:00:00
abstract::The tubular gland of the chicken oviduct is an attractive system for protein expression as large quantities of proteins are deposited in the egg, the production of eggs is easily scalable and good manufacturing practices for therapeutics from eggs have been established. Here we examined the ability of upstream and dow...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1132
更新日期:2005-09-01 00:00:00
abstract::Protein phosphorylation is a key post-translational modification regulating protein function in almost all cellular processes. Although tens of thousands of phosphorylation sites have been identified in human cells, approaches to determine the functional importance of each phosphosite are lacking. Here, we manually cu...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0344-3
更新日期:2020-03-01 00:00:00
abstract::Circular RNA transcripts were first identified in the early 1990s but knowledge of these species has remained limited, as their study through traditional methods of RNA analysis has been difficult. Now, novel bioinformatic approaches coupled with biochemical enrichment strategies and deep sequencing have allowed compr...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2890
更新日期:2014-05-01 00:00:00
abstract::We developed a modified flagellar type III secretion apparatus to secrete heterologous polypeptides into the growth medium of Escherichia coli. The secretion was facilitated by fusing the 173-bp untranslated DNA fragment upstream of the gene fliC (encoding flagellin) as well as a transcriptional terminator from fliC, ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1077
更新日期:2005-04-01 00:00:00
abstract::Peptide nucleic acids (PNAs) form stable and tight complexes with complementary DNA and/or RNA and would be promising antisense reagents if their cellular delivery could be improved. We show that a 21-mer PNA, complementary to the human galanin receptor type 1 mRNA, coupled to the cellular transporter peptides, transp...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0998-857
更新日期:1998-09-01 00:00:00
abstract::Insertional mutagenesis represents a major hurdle to gene therapy and necessitates sensitive preclinical genotoxicity assays. Cdkn2a-/- mice are susceptible to a broad range of cancer-triggering genetic lesions. We exploited hematopoietic stem cells from these tumor-prone mice to assess the oncogenicity of prototypica...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1216
更新日期:2006-06-01 00:00:00
abstract::Selectable marker genes are required to ensure the efficient genetic modification of crops. Economic incentives and safety concerns have prompted the development of several strategies (site-specific recombination, homologous recombination, transposition, and co-transformation) to eliminate these genes from the genome ...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt0602-575
更新日期:2002-06-01 00:00:00
abstract::Foxtail millet (Setaria italica), a member of the Poaceae grass family, is an important food and fodder crop in arid regions and has potential for use as a C(4) biofuel. It is a model system for other biofuel grasses, including switchgrass and pearl millet. We produced a draft genome (∼423 Mb) anchored onto nine chrom...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2195
更新日期:2012-05-13 00:00:00
abstract::Changes in glycosylation are often associated with disease progression, but the genetic and metabolic basis of these events is rarely understood in detail at a molecular level. We describe a metabolism-based approach to the selection of mutants in glycoconjugate biosynthesis that provides insight into regulatory mecha...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/89305
更新日期:2001-06-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is an essential regulator of normal and abnormal blood vessel growth. A monoclonal antibody (mAb) that targets VEGF suppresses tumor growth in murine cancer models and human patients. We investigated cellular and molecular events that mediate refractoriness of tumors to anti-a...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1323
更新日期:2007-08-01 00:00:00
abstract::The biological and physical sciences share a common interest in small structures (the definition of 'small' depends on the application, but can range from 1 nm to 1 mm). A vigorous trade across the borders of these areas of science is developing around new materials and tools (largely from the physical sciences) and n...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt872
更新日期:2003-10-01 00:00:00
abstract::Antisense oligonucleotides are designed to specifically hybridize to a target messenger RNA (mRNA) and interfere with the synthesis of the encoded protein. Uniformly modified oligonucleotides containing N3'-P5' phosphoramidate linkages exhibit (NP) extremely high-affinity binding to single-stranded RNA, do not induce ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/83489
更新日期:2001-01-01 00:00:00
abstract::The side chain of the antifungal antibiotic ansatrienin A from Streptomyces collinus contains a cyclohexanecarboxylic acid (CHC)-derived moiety. This moiety is also observed in trace amounts of omega-cyclohexyl fatty acids (typically less than 1% of total fatty acids) produced by S. collinus. Coenzyme A-activated CHC ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/79479
更新日期:2000-09-01 00:00:00
abstract::We describe wavelength-shifting molecular beacons, which are nucleic acid hybridization probes that fluoresce in a variety of different colors, yet are excited by a common monochromatic light source. The twin functions of absorption of energy from the excitation light and emission of that energy in the form of fluores...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/81192
更新日期:2000-11-01 00:00:00
abstract::The oncofetal fibronectin (B-FN) isoform is present in vessels of neoplastic tissues during angiogenesis but not in mature vessels. B-FN could therefore provide a target for diagnostic imaging and therapy of cancer. Phage display libraries have been used to isolate human antibody fragments with pan-species recognition...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt1197-1271
更新日期:1997-11-01 00:00:00
abstract::High-density short oligonucleotide microarrays have become a widely used tool for measuring gene expression on a large scale. However, details of the mechanism of binding on microarrays remain unclear. Short oligonucleotide probes currently synthesized on microarrays are often ineffective as a result of limited sequen...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt836
更新日期:2003-07-01 00:00:00
abstract::Standardized benchmarking approaches are required to assess the accuracy of variants called from sequence data. Although variant-calling tools and the metrics used to assess their performance continue to improve, important challenges remain. Here, as part of the Global Alliance for Genomics and Health (GA4GH), we pres...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-019-0054-x
更新日期:2019-05-01 00:00:00
abstract::We established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a ...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3353
更新日期:2015-10-01 00:00:00
abstract::Escherichia coli-based artificial chromosomes have become important tools for physical mapping and sequencing in various genome projects. The lack of a general method to modify these large bacterial clones, however, has limited their utility in functional studies. We developed a simple method to modify bacterial artif...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0997-859
更新日期:1997-09-01 00:00:00
abstract::The functional state of a cell is largely determined by the spatiotemporal organization of its proteome. Technologies exist for measuring particular aspects of protein turnover and localization, but comprehensive analysis of protein dynamics across different scales is possible only by combining several methods. Here w...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.2281
更新日期:2012-06-24 00:00:00
abstract::Genome editing with targeted nucleases and DNA donor templates homologous to the break site has proven challenging in human hematopoietic stem and progenitor cells (HSPCs), and particularly in the most primitive, long-term repopulating cell population. Here we report that combining electroporation of zinc finger nucle...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt.3408
更新日期:2015-12-01 00:00:00
abstract::Centromeric variation has been linked to cancer and infertility, but centromere sequences contain multiple tandem repeats and can only be assembled manually from long error-prone reads. Here we describe the centroFlye algorithm for centromere assembly using long error-prone reads, and apply it to assemble human centro...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/s41587-020-0582-4
更新日期:2020-11-01 00:00:00
abstract::To select for bacterial strains with enhanced phenotypes, random fragments of a whole genome, or a defined region of the genome, are cloned in a nonreplicating vector. The resulting plasmids are integrated by recombination into the homologous DNA region of the original strain. Integration gives rise to a nontandem dir...
journal_title:Nature biotechnology
pub_type: 杂志文章
doi:10.1038/nbt0697-564
更新日期:1997-06-01 00:00:00
abstract::Nucleotide variants, especially those related to epigenetic functions, provide critical regulatory information beyond simple genomic sequence, and they define cell status in higher organisms. 5-Methylcytosine, which is found in DNA, was until recently the only nucleotide variant studied in terms of epigenetics in euka...
journal_title:Nature biotechnology
pub_type: 杂志文章,评审
doi:10.1038/nbt.2398
更新日期:2012-11-01 00:00:00