Homology-driven genome editing in hematopoietic stem and progenitor cells using ZFN mRNA and AAV6 donors.

abstract：:Fluorescence microscopy of living cells enables visualization of the dynamics and interactions of intracellular molecules. However, fluorescence live-cell imaging is limited by photobleaching and phototoxicity induced by the excitation light. Here we describe controlled light-exposure microscopy (CLEM), a simple imagi...

journal_title：Nature biotechnology

authors： Hoebe RA,Van Oven CH,Gadella TW Jr,Dhonukshe PB,Van Noorden CJ,Manders EM

更新日期：2007-02-01 00:00:00

abstract：:Methods used to sequence the transcriptome often produce more than 200 million short sequences. We introduce StringTie, a computational method that applies a network flow algorithm originally developed in optimization theory, together with optional de novo assembly, to assemble these complex data sets into transcripts...

journal_title：Nature biotechnology

authors： Pertea M,Pertea GM,Antonescu CM,Chang TC,Mendell JT,Salzberg SL

更新日期：2015-03-01 00:00:00

abstract：:Changes in glycosylation are often associated with disease progression, but the genetic and metabolic basis of these events is rarely understood in detail at a molecular level. We describe a metabolism-based approach to the selection of mutants in glycoconjugate biosynthesis that provides insight into regulatory mecha...

journal_title：Nature biotechnology

authors： Yarema KJ,Goon S,Bertozzi CR

更新日期：2001-06-01 00:00:00

abstract：:A T-cell receptor mimic peptide (TCRpep) consisting of an 8-amino-acid peptide, homologous to the transmembrane region of the T-cell receptor (TCR) alpha chain, blocks T-cell activation after systemic application. When dendritic cells (DCs) were transduced to secrete the TCRpep and injected into mice, evidence of immu...

journal_title：Nature biotechnology

authors： Mahnke K,Qian Y,Knop J,Enk AH

更新日期：2003-08-01 00:00:00

abstract：:Isoflavones have drawn much attention because of their benefits to human health. These compounds, which are produced almost exclusively in legumes, have natural roles in plant defense and root nodulation. Isoflavone synthase catalyzes the first committed step of isoflavone biosynthesis, a branch of the phenylpropanoid...

journal_title：Nature biotechnology

authors： Jung W,Yu O,Lau SM,O'Keefe DP,Odell J,Fader G,McGonigle B

更新日期：2000-02-01 00:00:00

abstract：:Prediction of HLA epitopes is important for the development of cancer immunotherapies and vaccines. However, current prediction algorithms have limited predictive power, in part because they were not trained on high-quality epitope datasets covering a broad range of HLA alleles. To enable prediction of endogenous HLA ...

journal_title：Nature biotechnology

authors： Sarkizova S,Klaeger S,Le PM,Li LW,Oliveira G,Keshishian H,Hartigan CR,Zhang W,Braun DA,Ligon KL,Bachireddy P,Zervantonakis IK,Rosenbluth JM,Ouspenskaia T,Law T,Justesen S,Stevens J,Lane WJ,Eisenhaure T,Lan Zhang G,

更新日期：2020-02-01 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Eisenstein M

更新日期：2020-05-01 00:00:00

abstract：:Artificial transcription factors containing designer zinc-finger DNA-binding domains (DBDs) have been used to activate or repress expression of a growing number of endogenous genes. We have combined targeted zinc-finger DBD technology with a dimerizer-regulated gene expression system to permit the small-molecule contr...

journal_title：Nature biotechnology

authors： Pollock R,Giel M,Linher K,Clackson T

更新日期：2002-07-01 00:00:00

abstract：:The epicardium and its derivatives provide trophic and structural support for the developing and adult heart. Here we tested the ability of human embryonic stem cell (hESC)-derived epicardium to augment the structure and function of engineered heart tissue in vitro and to improve efficacy of hESC-cardiomyocyte grafts ...

journal_title：Nature biotechnology

authors： Bargehr J,Ong LP,Colzani M,Davaapil H,Hofsteen P,Bhandari S,Gambardella L,Le Novère N,Iyer D,Sampaziotis F,Weinberger F,Bertero A,Leonard A,Bernard WG,Martinson A,Figg N,Regnier M,Bennett MR,Murry CE,Sinha S

更新日期：2019-08-01 00:00:00

abstract：:We describe a simple technology used to cure an established metastatic disease. Intradermal injection of plasmid DNA encoding a transcriptionally targeted cytotoxic gene, along with hsp70, not only promoted tissue-specific, inflammatory killing of normal melanocytes, but also induced a CD8(+) T-cell-dependent, antigen...

journal_title：Nature biotechnology

authors： Daniels GA,Sanchez-Perez L,Diaz RM,Kottke T,Thompson J,Lai M,Gough M,Karim M,Bushell A,Chong H,Melcher A,Harrington K,Vile RG

更新日期：2004-09-01 00:00:00

abstract：:Little is known about the potential of plastids to accomplish post-translational modifications of foreign proteins. In the present study we generated transplastomic tobacco plants that accumulate the outer surface lipoprotein A (OspA)-the basic constituent of the first generation monovalent human vaccine against Lyme ...

journal_title：Nature biotechnology

authors： Glenz K,Bouchon B,Stehle T,Wallich R,Simon MM,Warzecha H

更新日期：2006-01-01 00:00:00

abstract：:Wild relatives of crops are an important source of genetic diversity for agriculture, but their gene repertoire remains largely unexplored. We report the establishment and analysis of a pan-genome of Glycine soja, the wild relative of cultivated soybean Glycine max, by sequencing and de novo assembly of seven phylogen...

journal_title：Nature biotechnology

authors： Li YH,Zhou G,Ma J,Jiang W,Jin LG,Zhang Z,Guo Y,Zhang J,Sui Y,Zheng L,Zhang SS,Zuo Q,Shi XH,Li YF,Zhang WK,Hu Y,Kong G,Hong HL,Tan B,Song J,Liu ZX,Wang Y,Ruan H,Yeung CK,Liu J,Wang H,Zhang LJ,Guan RX

更新日期：2014-10-01 00:00:00

abstract：:We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (VH:VL) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly ...

journal_title：Nature biotechnology

authors： Wang B,DeKosky BJ,Timm MR,Lee J,Normandin E,Misasi J,Kong R,McDaniel JR,Delidakis G,Leigh KE,Niezold T,Choi CW,Viox EG,Fahad A,Cagigi A,Ploquin A,Leung K,Yang ES,Kong WP,Voss WN,Schmidt AG,Moody MA,Ambrozak DR

更新日期：2018-02-01 00:00:00

abstract：:The oncofetal fibronectin (B-FN) isoform is present in vessels of neoplastic tissues during angiogenesis but not in mature vessels. B-FN could therefore provide a target for diagnostic imaging and therapy of cancer. Phage display libraries have been used to isolate human antibody fragments with pan-species recognition...

journal_title：Nature biotechnology

authors： Neri D,Carnemolla B,Nissim A,Leprini A,Querzè G,Balza E,Pini A,Tarli L,Halin C,Neri P,Zardi L,Winter G

更新日期：1997-11-01 00:00:00

abstract：:We developed a modified flagellar type III secretion apparatus to secrete heterologous polypeptides into the growth medium of Escherichia coli. The secretion was facilitated by fusing the 173-bp untranslated DNA fragment upstream of the gene fliC (encoding flagellin) as well as a transcriptional terminator from fliC, ...

journal_title：Nature biotechnology

authors： Majander K,Anton L,Antikainen J,Lång H,Brummer M,Korhonen TK,Westerlund-Wikström B

更新日期：2005-04-01 00:00:00

abstract：:Development of a cell therapy for diabetes would be greatly aided by a renewable supply of human beta-cells. Here we show that pancreatic endoderm derived from human embryonic stem (hES) cells efficiently generates glucose-responsive endocrine cells after implantation into mice. Upon glucose stimulation of the implant...

journal_title：Nature biotechnology

authors： Kroon E,Martinson LA,Kadoya K,Bang AG,Kelly OG,Eliazer S,Young H,Richardson M,Smart NG,Cunningham J,Agulnick AD,D'Amour KA,Carpenter MK,Baetge EE

更新日期：2008-04-01 00:00:00

abstract：:We introduce Sailfish, a computational method for quantifying the abundance of previously annotated RNA isoforms from RNA-seq data. Because Sailfish entirely avoids mapping reads, a time-consuming step in all current methods, it provides quantification estimates much faster than do existing approaches (typically 20 ti...

journal_title：Nature biotechnology

authors： Patro R,Mount SM,Kingsford C

更新日期：2014-05-01 00:00:00

abstract：:Reengineering the receptor footprints of adeno-associated virus (AAV) isolates may yield variants with improved properties for clinical applications. We generated a panel of synthetic AAV2 vectors by replacing a hexapeptide sequence in a previously identified heparan sulfate receptor footprint with corresponding resid...

journal_title：Nature biotechnology

authors： Asokan A,Conway JC,Phillips JL,Li C,Hegge J,Sinnott R,Yadav S,DiPrimio N,Nam HJ,Agbandje-McKenna M,McPhee S,Wolff J,Samulski RJ

更新日期：2010-01-01 00:00:00

abstract：:We established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a ...

journal_title：Nature biotechnology

authors： Xiao L,Feng Q,Liang S,Sonne SB,Xia Z,Qiu X,Li X,Long H,Zhang J,Zhang D,Liu C,Fang Z,Chou J,Glanville J,Hao Q,Kotowska D,Colding C,Licht TR,Wu D,Yu J,Sung JJ,Liang Q,Li J,Jia H,Lan Z,Tremaroli V,Dworzyn

更新日期：2015-10-01 00:00:00

abstract：:How intrinsic gene-regulatory networks interact with a cell's spatial environment to define its identity remains poorly understood. We developed an approach to distinguish between intrinsic and extrinsic effects on global gene expression by integrating analysis of sequencing-based and imaging-based single-cell transcr...

journal_title：Nature biotechnology

authors： Zhu Q,Shah S,Dries R,Cai L,Yuan GC

更新日期：2018-10-29 00:00:00

abstract：:RNA-guided gene drives capable of spreading genomic alterations made in laboratory organisms through wild populations could be used to address environmental and public health problems. However, the possibility of unintended genome editing occurring through the escape of strains from laboratories, coupled with the pros...

journal_title：Nature biotechnology

authors： DiCarlo JE,Chavez A,Dietz SL,Esvelt KM,Church GM

更新日期：2015-12-01 00:00:00

abstract：:We describe tethered conformation capture (TCC), a method for genome-wide mapping of chromatin interactions. By performing ligations on solid substrates rather than in solution, TCC substantially enhances the signal-to-noise ratio, thereby facilitating a detailed analysis of interactions within and between chromosomes...

journal_title：Nature biotechnology

authors： Kalhor R,Tjong H,Jayathilaka N,Alber F,Chen L

更新日期：2011-12-25 00:00:00

abstract：:Antisense oligonucleotides are designed to specifically hybridize to a target messenger RNA (mRNA) and interfere with the synthesis of the encoded protein. Uniformly modified oligonucleotides containing N3'-P5' phosphoramidate linkages exhibit (NP) extremely high-affinity binding to single-stranded RNA, do not induce ...

journal_title：Nature biotechnology

authors： Faria M,Spiller DG,Dubertret C,Nelson JS,White MR,Scherman D,Hélène C,Giovannangeli C

更新日期：2001-01-01 00:00:00

abstract：:Massively parallel sequencing of cDNA has enabled deep and efficient probing of transcriptomes. Current approaches for transcript reconstruction from such data often rely on aligning reads to a reference genome, and are thus unsuitable for samples with a partial or missing reference genome. Here we present the Trinity...

journal_title：Nature biotechnology

authors： Grabherr MG,Haas BJ,Yassour M,Levin JZ,Thompson DA,Amit I,Adiconis X,Fan L,Raychowdhury R,Zeng Q,Chen Z,Mauceli E,Hacohen N,Gnirke A,Rhind N,di Palma F,Birren BW,Nusbaum C,Lindblad-Toh K,Friedman N,Regev A

更新日期：2011-05-15 00:00:00

abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

journal_title：Nature biotechnology

authors： Qu L,Yi Z,Zhu S,Wang C,Cao Z,Zhou Z,Yuan P,Yu Y,Tian F,Liu Z,Bao Y,Zhao Y,Wei W

更新日期：2019-11-01 00:00:00

abstract：:Cellular function is critically regulated through degradation of substrates by the proteasome. To enable direct analysis of naturally cleaved proteasomal peptides under physiological conditions, we developed mass spectrometry analysis of proteolytic peptides (MAPP), a method for proteasomal footprinting that allows fo...

journal_title：Nature biotechnology

authors： Wolf-Levy H,Javitt A,Eisenberg-Lerner A,Kacen A,Ulman A,Sheban D,Dassa B,Fishbain-Yoskovitz V,Carmona-Rivera C,Kramer MP,Nudel N,Regev I,Zahavi L,Elinger D,Kaplan MJ,Morgenstern D,Levin Y,Merbl Y

更新日期：2018-10-22 00:00:00

abstract：:Efficiently generating evidence of clinical utility is a major challenge for ensuring clinical adoption of valuable diagnostics. A new approach to reimbursement in the United States offers a balance between evidence and incentives for molecular diagnostic tests. ...

journal_title：Nature biotechnology

authors： Schulman KA,Tunis SR

更新日期：2010-11-01 00:00:00

abstract：:Preeclampsia is a placentally induced hypertensive disorder of pregnancy that is associated with substantial morbidity and mortality to mothers and fetuses. Clinical manifestations of preterm preeclampsia result from excess circulating soluble vascular endothelial growth factor receptor FLT1 (sFLT1 or sVEGFR1) of plac...

journal_title：Nature biotechnology

authors： Turanov AA,Lo A,Hassler MR,Makris A,Ashar-Patel A,Alterman JF,Coles AH,Haraszti RA,Roux L,Godinho BMDC,Echeverria D,Pears S,Iliopoulos J,Shanmugalingam R,Ogle R,Zsengeller ZK,Hennessy A,Karumanchi SA,Moore MJ,Khvoro

更新日期：2018-11-19 00:00:00

abstract：:Systemic gene delivery into muscle has been a major challenge for muscular dystrophy gene therapy, with capillary blood vessels posing the principle barrier and limiting vector dissemination. Previous efforts to deliver genes into multiple muscles have relied on isolated vessel perfusion or pharmacological interventio...

journal_title：Nature biotechnology

authors： Wang Z,Zhu T,Qiao C,Zhou L,Wang B,Zhang J,Chen C,Li J,Xiao X

更新日期：2005-03-01 00:00:00

abstract：:In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two alternatively spliced transcripts, dsx-female (AgdsxF) and dsx-male (AgdsxM), that control differentiation of the two sexes. The female transcript, unlike the male, contains an exon (exon 5) whose sequence is highly conserved in all ...

journal_title：Nature biotechnology

authors： Kyrou K,Hammond AM,Galizi R,Kranjc N,Burt A,Beaghton AK,Nolan T,Crisanti A

更新日期：2018-12-01 00:00:00