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Data storage in DNA with fewer synthesis cycles using composite DNA letters.

Abstract：

:The density and long-term stability of DNA make it an appealing storage medium, particularly for long-term data archiving. Existing DNA storage technologies involve the synthesis and sequencing of multiple nominally identical molecules in parallel, resulting in information redundancy. We report the development of encoding and decoding methods that exploit this redundancy using composite DNA letters. A composite DNA letter is a representation of a position in a sequence that consists of a mixture of all four DNA nucleotides in a predetermined ratio. Our methods encode data using fewer synthesis cycles. We encode 6.4 MB into composite DNA, with distinguishable composition medians, using 20% fewer synthesis cycles per unit of data, as compared to previous reports. We also simulate encoding with larger composite alphabets, with distinguishable composition deciles, to show that 75% fewer synthesis cycles are potentially sufficient. We describe applicable error-correcting codes and inference methods, and investigate error patterns in the context of composite DNA letters.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Anavy L,Vaknin I,Atar O,Amit R,Yakhini Z

doi

10.1038/s41587-019-0240-x

subject

Has Abstract

pub_date

2019-10-01 00:00:00

pages

1229-1236

issue

10

eissn

1087-0156

issn

1546-1696

pii

10.1038/s41587-019-0240-x

journal_volume

37

pub_type

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