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High-resolution genome-wide mapping of histone modifications.

Abstract：

:The expression patterns of eukaryotic genomes are controlled by their chromatin structure, consisting of nucleosome subunits in which DNA of approximately 146 bp is wrapped around a core of 8 histone molecules. Post-translational histone modifications play an essential role in modifying chromatin structure. Here we apply a combination of SAGE and chromatin immunoprecipitation (ChIP) protocols to determine the distribution of hyperacetylated histones H3 and H4 in the Saccharomyces cerevisiae genome. We call this approach genome-wide mapping technique (GMAT). Using GMAT, we find that the highest acetylation levels are detected in the 5' end of a gene's coding region, but not in the promoter. Furthermore, we show that the histone acetyltransferase, GCN5p, regulates H3 acetylation in the promoter and 5' end of the coding regions. These findings indicate that GMAT should find valuable applications in mapping target sites of chromatin-modifying enzymes.

journal_name

Nat Biotechnol

journal_title

Nature biotechnology

authors

Roh TY,Ngau WC,Cui K,Landsman D,Zhao K

doi

10.1038/nbt990

subject

Has Abstract

pub_date

2004-08-01 00:00:00

pages

1013-6

issue

8

eissn

1087-0156

issn

1546-1696

pii

nbt990

journal_volume

22

pub_type

杂志文章

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