Abstract：
:In order to analyse gene expression during fruit body development of the ectomychorrizal fungus Tuber borchii Vittad., a modified differential display procedure was set up. The procedure used is easier and faster than the traditional one and generates reproducible cDNA banding patterns that can be resolved on a standard ethidium bromide-agarose gel. From 16 cDNA fingerprints, 25 amplicons with apparent differential expression were identified and cloned without a previous reamplification. Fifteen clones showed significant similarity to known proteins that are involved in dikaryosis and fruiting, cell division, transport across membranes, mitochondrial division, intermediary metabolism, biosynthesis of isoprenoid compounds and putative RNA/DNA binding. Northern blot analyses confirmed that seven cDNAs were indeed differentially expressed during fruit body development. The characterisation of these cDNAs represents a starting point in understanding the molecular mechanisms of cellular differentiation leading to the development of the T. borchii fruit body.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Zeppa S,Guidi C,Zambonelli A,Potenza L,Vallorani L,Pierleoni R,Sacconi C,Stocchi Vdoi
10.1007/s00294-002-0343-6subject
Has Abstractpub_date
2002-12-01 00:00:00pages
161-8issue
3eissn
0172-8083issn
1432-0983journal_volume
42pub_type
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