Abstract:
:Approximately, 1% of the genes in eukaryotic genomes encode for helicases, which make the number of helicases expressed in the cell considerably high. Helicases are motor proteins that participate in many central aspects of the nuclear and mitochondrial genomes, and based on their helicase motif conservation, they are divided into different helicase families. The Pif1 family of helicases is an evolutionarily conserved helicase family that is associated with familial breast cancer in humans. The Schizosaccharomyces pombe Pfh1 helicase belongs to the Pif1 helicase family and is a multi-tasking helicase that is important for replication fork progression through natural fork barriers, for G-quadruplex unwinding, and for Okazaki fragment maturation, and these activities are potentially shared by the human Pif1 helicase. This review discusses the known functions of the Pfh1 helicase, the study of which has led to a better understanding of nucleic acid metabolism in eukaryotes.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Sabouri Ndoi
10.1007/s00294-016-0675-2subject
Has Abstractpub_date
2017-08-01 00:00:00pages
621-626issue
4eissn
0172-8083issn
1432-0983pii
10.1007/s00294-016-0675-2journal_volume
63pub_type
杂志文章,评审相关文献
CURRENT GENETICS文献大全abstract::The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00336850
更新日期:1992-03-01 00:00:00
abstract::A model for the recombination-directed replication and amplification of the mtDNA of amphimeric petite mutants of S. cerevisiae is proposed. Replication of an amphimeric master basic unit datA would be initiated in the inverted components a and A. The initiation of replication should be associated with the amphimeric ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050112
更新日期:1996-07-31 00:00:00
abstract::The physical positions of the DNA sequences encoding the five consecutive enzyme activities required to metabolise 3-deoxy-D-arabino-heptulosonic acid-7-phosphate to 5-enolpyruvyl-shikimate-3phosphate, which are encoded by the A. nidulans Arom polypeptide have been determined. Subfragments of the Arom locus encoding E...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00384611
更新日期:1987-01-01 00:00:00
abstract::Unlike most polymorphic markers in the Chlamydomonas eugametos and Chlamydomonas moewusii chloroplast DNAs (cpDNAs), the C. moewusii 6- and 21-kb extra sequences and the C. eugametos-specific CeLSU small middle dot 5 intron are transmitted to all of the few viable progeny in reciprocal crosses between the two green al...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050144
更新日期:1996-09-01 00:00:00
abstract::Expression of pho1-encoded acid phosphatase of Schizosaccharomyces pombe has been reported to be regulated by phosphate. In this communication we show that it is also regulated by adenine. Starving adenine auxotrophic strains for adenine leads to a drastic increase of the enzymatic activity while adenine represses thi...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00317923
更新日期:1992-10-01 00:00:00
abstract::Stress granules (SGs) are evolutionarily conserved ribonucleoprotein (RNP) structures that form in response to a variety of environmental and cellular cues. The presence of these RNP granules has been linked to a number of human diseases, including neurodegenerative disorders like amyotrophic lateral sclerosis (ALS) a...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0571-9
更新日期:2016-08-01 00:00:00
abstract::Full genome recoding, or rewriting codon meaning, through chemical synthesis of entire bacterial chromosomes has become feasible in the past several years. Recoding an organism can impart new properties including non-natural amino acid incorporation, virus resistance, and biocontainment. The estimated cost of construc...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-017-0754-z
更新日期:2018-04-01 00:00:00
abstract::A previously unknown nuclear gene DSS-1 from Saccharomyces cerevisiae was cloned and sequenced. The gene was isolated as a multicopy suppressor of a disruption of the SUV-3 gene coding for a DEAD/H box protein involved in processing and turnover of mitochondrial transcripts. The DSS-1 gene codes for a 970 amino-acid p...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00315775
更新日期:1995-07-01 00:00:00
abstract::The mitochondrial genomes are one of the most information-rich markers in phylogenetics. The relationships within superorder Dictyoptera have been debated in the literature. However, the closely related termites (Isoptera) are retained as unranked taxon within the order Blattaria (cockroaches). In this work, we sequen...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-012-0365-7
更新日期:2012-04-01 00:00:00
abstract::Chitin is a component of the yeast cell wall which is localized to the septum between mother and daughter cells. Previous work in Saccharomyces cerevisiae has shown that this organism possesses three chitin synthases, 1, 2, and 3. Disruption experiments have shown that loss of chitin synthase 2 has a more profound eff...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00352007
更新日期:1993-02-01 00:00:00
abstract::Although cell division is usually portrayed as an equitable process by which a progenitor cell originates two identical daughter cells, there are multiple examples of asymmetric divisions that generate two cells that differ in their content, morphology and/or proliferative potential. The capacity of the cells to gener...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-020-01074-y
更新日期:2020-08-01 00:00:00
abstract::The circular mitochondrial genome of Dictyostelium discoideum has a size of 55,564 base pairs. We present here a complete and detailed transcription map of the mitochondrial DNA. Eight major, polycistronic transcripts encoding polypeptides, ribosomal RNAs and interspersed transfer RNAs were identified in Northern hybr...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940100196
更新日期:2001-07-01 00:00:00
abstract::Caffeine is a well known base analogue and is cytotoxic to both animal and yeast cells. There are two possible mechanisms by which yeast cells tolerate caffeine concentrations higher than normal, by mutation or by physiological adaptation. We have isolated novel caffeine-resistant mutants of S. pombe which define thre...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050233
更新日期:1997-06-01 00:00:00
abstract::The atpB and atpE genes encode beta and epsilon subunits, respectively, of chloroplast ATP synthase and are co-transcribed in the plant species so far studied. In tobacco, an atpB gene-specific probe hybridizes to 2.7- and 2.3-kb transcripts. In addition to these, a probe from the atpE coding region hybridizes also to...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309558
更新日期:1994-09-01 00:00:00
abstract::The generation of a comprehensive EST library and the sequencing of its genome set the stage for reverse genetics approaches in Chlamydomonas reinhardtii. However, these also require tools for the specific downregulation of target gene expression. Consequently, a large number of diverse constructs were developed aimed...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-005-0042-1
更新日期:2006-02-01 00:00:00
abstract::In Chlamydomonas reinhardtii, the expression of the nit1 gene encoding nitrate reductase is dependent on the nature of the nitrogen source and on other environmental factors. We have fused the nit1 promoter region to the arylsulphatase (ars) reporter gene lacking its own promoter and introduced this chimeric construct...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050204
更新日期:1997-03-01 00:00:00
abstract::Mitochondrial (mt) DNA structures were studied in 12 nucleus-cytoplasm (NC) hybrids of tetraploid and hexaploid wheat in which nuclear genomes of the recurrent paternal wheat parents were combined with D and D2 plasmons from Aegilops species. RFLP analysis of 15 mtDNA regions indicated the presence of the paternal seq...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940000153
更新日期:2000-11-01 00:00:00
abstract::NRE, the nitrogen regulatory protein of Penicillium chrysogenum, contains a single Cys2/Cys2-type zinc-finger motif followed immediately by a highly basic region. The zinc-finger domain was expressed to Escherichia coli as a fusion protein with beta-galactosidase. In order to test the putative DNA-binding ability of N...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00315785
更新日期:1995-07-01 00:00:00
abstract::We have isolated suppressors of a PHO4c (a positive regulator) mutant which normally confers weak constitutivity for acid phosphatase production on the Saccharomyces cell. One dominant suppressor (PHO80-2) was found to be an allele of PHO80 (a negative regulator) that changes G to A, resulting in substitution of isole...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00318466
更新日期:1992-02-01 00:00:00
abstract::Timely progression of living cells through the cell cycle is precisely regulated. This involves a series of phosphorylation events which are regulated by various cyclins, activated in coordination with the cell cycle progression. Phosphorylated proteins govern cell growth, division as well as duplication of the geneti...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-017-0706-7
更新日期:2017-12-01 00:00:00
abstract::In filamentous ascomycetes, glucose repression is mediated by CRE1, a zinc-finger protein related to Miglp from yeast. Five putative AMPK phosphorylation motifs identified in the glucose repressor from the phytopathogenic fungus Sclerotinia sclerotiorum were mutated in a GFP::CRE1 translational fusion. Complementation...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050535
更新日期:2000-05-01 00:00:00
abstract::SecA is one of seven Sec proteins that comprise the prokaryotic protein translocation apparatus. A chloroplast-encoded secA gene has been identified from the unicellular chromophytic alga Pavlova lutherii. The gene predicts a protein that is related to the SecA proteins of Escherichia coli and Bacillus subtilis. The p...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00352444
更新日期:1992-11-01 00:00:00
abstract::An Agrocybe aegerita cDNA library, constructed from fruit body primordia poly(A)+ RNAs, was screened by differential colony hybridization. Clones which preferentially hybridized to poly(A)+ RNA sequences from fruit body primordia, versus poly(A)+ RNAs from mycelium, were isolated. Eight of these clones (EMAa-1 to EMAa...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326234
更新日期:1991-08-01 00:00:00
abstract::Adaptation by natural selection might improve the fitness of an organism and its probability to survive in unfavorable environmental conditions. Decoding the genetic basis of adaptive evolution is one of the great challenges to deal with. To this purpose, Saccharomyces cerevisiae has been largely investigated because ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-016-0635-x
更新日期:2017-05-01 00:00:00
abstract::The isoprenoid pathway of the ectomycorrhizal fungus Tuber borchii Vittad is investigated to better understand the molecular mechanisms at work, in particular during the maturation of the complex ascomata (the so-called "truffles"). Three T. borchii genes coding for the most important regulatory enzymes of the isopren...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0097-7
更新日期:2006-12-01 00:00:00
abstract::The directed and controlled switching of mating type which occurs in homothallic forms of the yeast Saccharomyces cerevisiaee is dependent upon the presence of the wild type RAD52 gene. The RAD52 gene is also required for spontaneous mitotic and meiotic recombination. It has been observed that the two haploid mating t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00377609
更新日期:1983-11-01 00:00:00
abstract::The cytoplasmic virus-like element pWR1A from Debaryomyces robertsiae encodes a toxin (DrT) with similarities to the Pichia acaciae killer toxin PaT, which acts by importing a toxin subunit (PaOrf2) with tRNA anticodon nuclease activity into target cells. As for PaT, loss of the tRNA methyltransferase Trm9 or overexpr...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-014-0426-1
更新日期:2014-08-01 00:00:00
abstract::The ribosomal proteins of two ascomycetes Podospora anserina and Saccharomyces cerevisiae, were compared by two dimensional electrophoresis in two different gel systems and we found only five pairs of proteins which have kept homologous physico-chemical properties under these conditions. An immunological analysis was ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00396204
更新日期:1984-12-01 00:00:00
abstract::We report the nucleotide sequence of three tRNA genes from maize mitochondria. The genes are located in two BamHI fragments, 3.55 and 5.7 kb long, adjacent to the S2 sequence in the maize mitochondrial genome. On the 3.55 kb BamHI fragment, we have characterized a tRNA(Cys)(GCA) gene. A strong sequence homology of thi...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00387771
更新日期:1988-03-01 00:00:00
abstract::Some diploid strains of Saccharomyces cerevisiae can grow both as a spherical yeast form and as a filamentous pseudohyphal form. Most yeasts capable of forming pseudohyphae possess a functional FLO8 gene. We show that disrupting the ACE2 transcription factor results in the production of pseudohyphae in a flo8-1 backgr...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050384
更新日期:1998-09-01 00:00:00