Immunity factors for two related tRNAGln targeting killer toxins distinguish cognate and non-cognate toxic subunits.

Abstract:

:The cytoplasmic virus-like element pWR1A from Debaryomyces robertsiae encodes a toxin (DrT) with similarities to the Pichia acaciae killer toxin PaT, which acts by importing a toxin subunit (PaOrf2) with tRNA anticodon nuclease activity into target cells. As for PaT, loss of the tRNA methyltransferase Trm9 or overexpression of tRNA(Gln) increases DrT resistance and the amount of tRNA(Gln) is reduced upon toxin exposure or upon induced intracellular expression of the toxic DrT subunit gene DrORF3, indicating DrT and PaT to share the same in vivo target. Consistent with a specific tRNase activity of DrOrf3, the protein cleaves tRNA(Gln) but not tRNA(Glu) in vitro. Heterologous cytoplasmic expression identified DrOrf5 as the DrT specific immunity factor; it confers resistance to exogenous DrT as well as to intracellular expression of DrOrf3 and prevents tRNA depletion by the latter. The PaT immunity factor PaOrf4, a homologue of DrOrf5 disables intracellular action of both toxins. However, the DrT protection level mediated by PaOrf4 is reduced compared to DrOrf5, implying a recognition mechanism for the cognate toxic subunit, leading to incomplete toxicity suppression of similar, but non-cognate toxic subunits.

journal_name

Curr Genet

journal_title

Current genetics

authors

Klassen R,Kast A,Wünsche G,Paluszynski JP,Wemhoff S,Meinhardt F

doi

10.1007/s00294-014-0426-1

subject

Has Abstract

pub_date

2014-08-01 00:00:00

pages

213-22

issue

3

eissn

0172-8083

issn

1432-0983

journal_volume

60

pub_type

杂志文章
  • Structure of the rubisco operon from the multicellular red alga Antithamnion spec.

    abstract::In the multicellular red alga Antithamnion spec. both rubisco genes (rbcL and rbcS) are encoded on the plastid DNA (ptDNA). Both genes are separated by a short A/T-rich spacer of 100 bp and are cotranscribed into an mRNA of approximately 2.7 kb. These findings are in extensive agreement with those obtained from two un...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00309918

    authors: Kostrzewa M,Valentin K,Maid U,Radetzky R,Zetsche K

    更新日期:1990-12-01 00:00:00

  • Transformations of Penicillium islandicum and Penicillium frequentans that produce anthraquinone-related compounds.

    abstract::Wild-type strains of Penicillium islandicum and Penicillium frequentans, which produce anthraquinone and related compounds, were transformed to benomyl and hygromycin B resistance. Plasmids pSV50 and pBT6, with benomyl-resistant beta-tublin genes, and plasmids pAN7-1 and pDH25, with a bacterial hygromycin phosphotrans...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00518172

    authors: Huang KX,Iwakami N,Fujii I,Ebizuka Y,Sankawa U

    更新日期:1995-11-01 00:00:00

  • The complex Arom locus of Aspergillus nidulans. Evidence for multiple gene fusions and convergent evolution.

    abstract::The physical positions of the DNA sequences encoding the five consecutive enzyme activities required to metabolise 3-deoxy-D-arabino-heptulosonic acid-7-phosphate to 5-enolpyruvyl-shikimate-3phosphate, which are encoded by the A. nidulans Arom polypeptide have been determined. Subfragments of the Arom locus encoding E...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00384611

    authors: Hawkins AR

    更新日期:1987-01-01 00:00:00

  • Cointegration of transforming DNAs in Aspergillus nidulans: a model using autonomously-replicating plasmids.

    abstract::Transforming DNAs form cointegrates in Aspergillus nidulans by homologous and non-homologous recombination as well as by end-to-end ligation of linear fragments. This process has been studied by means of a model in which the linkage of a marker gene to the origin of autonomous replication AMA1 was selected for. Recomb...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00310500

    authors: Aleksenko AY

    更新日期:1994-10-01 00:00:00

  • Caffeine-resistance in S. pombe: mutations in three novel caf genes increase caffeine tolerance and affect radiation sensitivity, fertility, and cell cycle.

    abstract::Caffeine is a well known base analogue and is cytotoxic to both animal and yeast cells. There are two possible mechanisms by which yeast cells tolerate caffeine concentrations higher than normal, by mutation or by physiological adaptation. We have isolated novel caffeine-resistant mutants of S. pombe which define thre...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940050233

    authors: Benko Z,Miklos I,Carr AM,Sipiczki M

    更新日期:1997-06-01 00:00:00

  • Developmental and light-dependent cues interact to establish steady-state levels of transcripts for photosynthesis-related genes (psbA, psbD, psaA and rbcL) in rice (Oryza sativa L.).

    abstract::The steady-state transcript levels for psbA, psbD, psaA and rbcL are low in dark-grown rice seedlings as compared to those grown in light. Following seed germination, they accumulate in an age-dependent manner, in dark as well as light, reaching a maximal level on the 7th or 8th day, before a slow decline sets in. But...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00351491

    authors: Kapoor S,Maheshwari SC,Tyagi AK

    更新日期:1994-04-01 00:00:00

  • Mutational analysis of a variant of ARS1 from Saccharomyces cerevisiae.

    abstract::A naturally occurring single base-pair G to A transition, creating a 10/11 near-match close to the essential 11 base-pair core consensus of ARS1, was used to investigate the importance of near-match sequences. The 10/11 near-match can not substitute for the core consensus since an ARS- phenotype is observed when the c...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00351723

    authors: Kirpekar F,Gulløv K

    更新日期:1992-09-01 00:00:00

  • Characterization and prevalence of a circular mitochondrial plasmid in senescence-prone isolates of Neurospora intermedia.

    abstract::Genetic and molecular analyses of the phenomenon of senescence-i.e., irreversible loss of growth and reproductive potential upon subculturing-in Neurospora intermedia strain M1991-60A, collected from Maddur in southern India, showed the presence of plasmid pMaddur1, which is homologous to the senescence-inducing circu...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-004-0558-9

    authors: D'Souza AD,Sultana S,Maheshwari R

    更新日期:2005-03-01 00:00:00

  • Integrative and replicative genetic transformation of Aureobasidium pullulans.

    abstract::A hybrid selectable marker for transformation was constructed by placing the promoter (TEF1p) from the gene encoding the Aureobasidium pullulans translation elongation factor 1-alpha (TEF1) adjacent to the 5' end of the Escherichia coli hygromycin B phosphotransferase gene (HPT). Plasmids containing this hybrid gene (...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00313195

    authors: Thornewell SJ,Peery RB,Skatrud PL

    更新日期:1995-12-01 00:00:00

  • In vitro mutagenesis of the mitochondrial leucyl-tRNA synthetase of S. cerevisiae reveals residues critical for its in vivo activities.

    abstract::The mitochondrial leucyl-tRNA synthetase (mLRS) of Saccharomyces cerevisiae is involved in both mitochondrial protein synthesis and pre-mRNA splicing. We have created mutations in the regions HIGH, GWD and KMSKS, which are involved in ATP-, amino acid- and tRNA-binding respectively, and which have been conserved in th...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00351744

    authors: Li GY,Herbert CJ,Labouesse M,Slonimski PP

    更新日期:1992-07-01 00:00:00

  • Analysis of mitochondrial RNA in Saccharomyces cerevisiae.

    abstract::Mitochondrial RNA from grande yeast was analyzed by electrophoresis on agarose-urea, acrylamide-urea, and methyl mercuric hydroxide-agarose gels. These gel systems display more than 40 RNA bands that copurify with mitochondria; these bands are not present in cytoplasmic RNA preparations. Analysis of molecular weight o...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00446962

    authors: Locker J,Morimoto R,Synenki RM,Rabinowitz M

    更新日期:1980-02-01 00:00:00

  • Targeted disruption of a G protein alpha subunit gene results in reduced pathogenicity in Fusarium oxysporum.

    abstract::The cloning of fga1, the gene encoding a G protein alpha subunit, was performed by standard PCR techniques and by screening a Fusarium oxysporum genomic library, using the PCR product as a probe. The full-length open reading frame spanned 1,059 nucleotides and the deduced primary structure of the protein (353 amino ac...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-002-0322-y

    authors: Jain S,Akiyama K,Mae K,Ohguchi T,Takata R

    更新日期:2002-09-01 00:00:00

  • Analysis of the functional domains of the mismatch repair homologue Msh1p and its role in mitochondrial genome maintenance.

    abstract::Mitochondrial DNA (mtDNA) repair occurs in all eukaryotic organisms and is essential for the maintenance of mitochondrial function. Evidence from both humans and yeast suggests that mismatch repair is one of the pathways that functions in overall mtDNA stability. In the mitochondria of the yeast Saccharomyces cerevisi...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-004-0537-1

    authors: Mookerjee SA,Lyon HD,Sia EA

    更新日期:2005-02-01 00:00:00

  • Natural translocation of a large segment of chromosome III to chromosome I in a laboratory strain of Saccharomyces cerevisiae.

    abstract::We have investigated chromosomal segregation during meiosis in a cross between two polymorphic haploid laboratory strains of Saccharomyces cerevisiae, FL100 and GRF18. These two strains have large chromosome-length polymorphisms for chromosomes I and III allowing for easy scoring of parental chromosomes after meiotic ...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940050124

    authors: Camasses A

    更新日期:1996-08-01 00:00:00

  • Partial sequence of the mitochondrial genome of the crustacean Daphnia pulex.

    abstract::A 4062-nucleotide (nt) fragment of the mitochondrial genome of the crustacean Daphnia pulex was sequenced and found to contain the complete genes for eight tRNAs and five proteins (ATP6, ATP8, COII, COIII, ND3) and the partial sequence of COI. In combination with data described previously, approximately 50% of the D. ...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940050175

    authors: Crease TJ,Little TJ

    更新日期:1997-01-01 00:00:00

  • DNA Topoisomerase II modulates acetyl-regulation of cohesin-mediated chromosome dynamics.

    abstract::Cohesin is one of three multi-protein structural maintenance of chromosome (SMC) complexes that regulate eukaryotic chromosome dynamics. It forms a ring-shaped structure that embraces sister chromatids through interphase to promote their pairing. In preparation for mitosis, most cohesin is stripped from the chromosome...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-017-0691-x

    authors: Lin SJ,O'Connell MJ

    更新日期:2017-10-01 00:00:00

  • L1-like non-LTR retrotransposons in the yeast Candida albicans.

    abstract::Non-LTR retrotransposons (also known as LINEs) have had a major influence on the structure of many eukaryote genomes. They are abundant in many multicellular eukaryotes, including mammals, but appear to be absent from the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. This absence has, to date, preclud...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940000181

    authors: Goodwin TJ,Ormandy JE,Poulter RT

    更新日期:2001-04-01 00:00:00

  • Expression of the arylsulphatase reporter gene under the control of the nit1 promoter in Chlamydomonas reinhardtii.

    abstract::In Chlamydomonas reinhardtii, the expression of the nit1 gene encoding nitrate reductase is dependent on the nature of the nitrogen source and on other environmental factors. We have fused the nit1 promoter region to the arylsulphatase (ars) reporter gene lacking its own promoter and introduced this chimeric construct...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940050204

    authors: Ohresser M,Matagne RF,Loppes R

    更新日期:1997-03-01 00:00:00

  • Cac1 WHD and PIP domains have distinct roles in replisome progression and genomic stability.

    abstract::Replication-coupled (RC) nucleosome assembly is an essential process in eukaryotic cells to maintain chromatin structure during DNA replication. The deposition of newly-synthesized H3/H4 histones during DNA replication is facilitated by specialized histone chaperones. CAF-1 is an important histone chaperone complex an...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-020-01113-8

    authors: Tsirkas I,Dovrat D,Lei Y,Kalyva A,Lotysh D,Li Q,Aharoni A

    更新日期:2020-10-06 00:00:00

  • Volvox germline-specific genes that are putative targets of RegA repression encode chloroplast proteins.

    abstract::In Volvox carteri, regA acts as a master gene to suppress all germ cell functions in somatic cells. Its product, RegA, has features of a transcriptional repressor. Here we report cDNA sequences representing 15 nuclear genes with properties expected of RegA targets: they are expressed strongly in germ cells and in regA...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940050511

    authors: Meissner M,Stark K,Cresnar B,Kirk DL,Schmitt R

    更新日期:1999-12-01 00:00:00

  • Debaryomyces hansenii, a highly osmo-tolerant and halo-tolerant yeast, maintains activated Dhog1p in the cytoplasm during its growth under severe osmotic stress.

    abstract::The HOG pathway is an important mitogen-activated protein kinase (MAPK) signal transduction pathway in Saccharomyces cerevisiae that mediates adaptation of cells to hyper-osmotic stress. Activation of this pathway causes rapid but transient, phosphorylation of the MAPK Hog1p. Phosphorylated Hog1p is rapidly transporte...

    journal_title:Current genetics

    pub_type: 杂志文章,评审

    doi:10.1007/s00294-005-0010-9

    authors: Sharma P,Meena N,Aggarwal M,Mondal AK

    更新日期:2005-09-01 00:00:00

  • HBP2: a new mammalian protein that complements the fission yeast MBF transcription complex.

    abstract::The mammalian HBP2 gene has been isolated by functional complementation of cells unable to undergo DNA replication in fission yeast. HBP2 is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As in other members of the family, the HMG box in...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940100241

    authors: Sánchez-Díaz A,Blanco MA,Jones N,Moreno S

    更新日期:2001-09-01 00:00:00

  • Tuning the sequence specificity of a transcription terminator.

    abstract::The bacterial hexameric helicase known as Rho is an archetypal sequence-specific transcription terminator that typically halts the synthesis of a defined set of transcripts, particularly those bearing cytosine-rich 3'-untranslated regions. However, under conditions of translational stress, Rho can also terminate trans...

    journal_title:Current genetics

    pub_type: 杂志文章,评审

    doi:10.1007/s00294-019-00939-1

    authors: Lawson MR,Berger JM

    更新日期:2019-06-01 00:00:00

  • Diverse roles of Dpb2, the non-catalytic subunit of DNA polymerase ε.

    abstract::Timely progression of living cells through the cell cycle is precisely regulated. This involves a series of phosphorylation events which are regulated by various cyclins, activated in coordination with the cell cycle progression. Phosphorylated proteins govern cell growth, division as well as duplication of the geneti...

    journal_title:Current genetics

    pub_type: 杂志文章,评审

    doi:10.1007/s00294-017-0706-7

    authors: Dmowski M,Fijałkowska IJ

    更新日期:2017-12-01 00:00:00

  • Deletion of the Cochliobolus heterostrophus mating-type (MAT) locus promotes the function of MAT transgenes.

    abstract::Cochliobolus heterostrophus has alternate genes (MAT-1 and MAT-2) at its mating-type locus. Transformants of a MAT-1 or a MAT-2 strain carrying a transgene of opposite mating type can self and are dual maters; the transgene, however, promotes development of pseudothecia only, not ascospores. To determine if the reside...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:

    authors: Wirsel S,Turgeon BG,Yoder OC

    更新日期:1996-02-01 00:00:00

  • ALG/alg: a single gene controlling the utilization of lactate in the presence of antimycin in the yeast Saccharomyces cerevisiae.

    abstract::A strain dependent growth on lactate in the presence of antimycin A (AA) has been observed - the strain D261 can grow on lactate and AA, whereas in the strain K8/6C antimycin A prevents the utilization of lactate and the induction of LDH.Genetic analysis demonstrates that growth on lactate in the presence of AA segreg...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00433906

    authors: Ferrero I,Rambaldelli R,Genga AM,Donnini C,Puglisi PP

    更新日期:1984-08-01 00:00:00

  • The endpoints of an inversion in wheat chloroplast DNA are associated with short repeated sequences containing homology to att-lambda.

    abstract::The endpoints of an inversion in wheat chloroplast DNA are shown to be associated with copies of a short repeated sequence. Recombination across the repeats in an inverted configuration may have been responsible for the inversion, although they are currently in a direct orientation owing to a second inversion. The rep...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00636479

    authors: Howe CJ

    更新日期:1985-01-01 00:00:00

  • Rubisco spacer sequence divergence in the rhodophyte alga Gracilaria verrucosa and closely related species.

    abstract::In the red alga Gracilaria verrucosa, the genes encoding the large and the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) are separated by a short spacer of less than 131 bp. Sequencing of PCR-amplified Rubisco spacers from a number of populations of G. verrucosa was performed to assess the...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00309601

    authors: Destombe C,Douglas SE

    更新日期:1991-05-01 00:00:00

  • Isolation and characterisation of the mating-type (MAT) locus from Rhynchosporium secalis.

    abstract::The mating-type ( MAT) genes from Rhynchosporium secalis were isolated using PCR-based methods. Characterisation of the MAT idiomorphs suggests that R. secalis is closely related to the discomycetes Pyrenopeziza brassicae and Tapesia yallundae in terms of sequence and MAT locus gene composition. The MAT1-2 idiomorph c...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-003-0445-9

    authors: Foster SJ,Fitt BD

    更新日期:2003-12-01 00:00:00

  • Genetics of fruit body production in higher basidiomycetes II. Monokaryotic and dikaryotic fruiting in schizophyllum commune.

    abstract::In the wood destroying fungus Schizophyllum commune, a well known subject for genetic studies, fruit bodies are produced not only in the course of the sexual cycle but also asexually. Sexual fruiting requires the establishment of a dikaryon which is under the control of the incompatibility factors A and B. Asexual fru...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00413309

    authors: Esser K,Saleh F,Meinhardt F

    更新日期:1979-12-01 00:00:00