Genetics of fruit body production in higher basidiomycetes II. Monokaryotic and dikaryotic fruiting in schizophyllum commune.

abstract：:An electrophoretic karyotype of the filamentous fungus Podospora anserina has been obtained using contour-clamped homogeneous electric field gel electrophoresis. Six chromosomal bands were separated with one migrating as a doublet. The size of the chromosomes was estimated to be between 3.8 and 6.0 megabase pairs (mb)...

journal_title：Current genetics

authors： Javerzat JP,Jacquier C,Barreau C

更新日期：1993-09-01 00:00:00

abstract：:Meiotic fine-structure maps of two efficient UGA suppressors of Schizosaccharomyces pombe which are known (sup8-e) or inferred (sup10-e) to code for two leucine tRNAs carrying the mutant anticodon U(*)CA (Kohli et al. 1979, 1980a, b; Wetzel et al. 1979; Mao et al. 1981) are presented. In both cases, the recombination ...

journal_title：Current genetics

authors： Munz P,Dorsch-Häsler K,Leupold U

更新日期：1983-04-01 00:00:00

abstract：:In order to facilitate the process of epitope-tagging of yeast proteins, we have constructed two Saccharomyces cerevisiae-Escherichia coli shuttle vectors that allow fusion of a sequence encoding an epitope of the human c-myc protein at the 3' end of any gene. An example of the use of this technique is presented. ...

journal_title：Current genetics

authors： Reisdorf P,Maarse AC,Daignan-Fornier B

更新日期：1993-02-01 00:00:00

abstract：:We find three interesting characteristics of the genes encoding the ribosomal RNA (rRNA) in the basidiomycete Coprinus cinereus. First, there are only 60 to 90 copies of the genes, fewer than in other fungi. Second, the genes are organized in an unusual arrangement. The 5S rRNA genes are located in the repeat unit whi...

journal_title：Current genetics

authors： Rhodes Cassidy J,Moore D,Lu BC,Pukkila PJ

更新日期：1984-10-01 00:00:00

abstract：:Six ultra violet (UV) mutageneses were performed on the spo76 UV-sensitive mutant of Sordaria macrospora. Spo76 shows an early centromere cleavage associated with an arrest at the first meiotic division and therefore does not form ascospores. Moreover, it exhibits altered pairing structure (synaptonemal complex), reve...

journal_title：Current genetics

authors： Huynh AD,Leblon G,Zickler D

更新日期：1986-01-01 00:00:00

abstract：:Two types of DNA sequence polymorphisms were found among different geographic isolates of the basidiomycete fungus Coprinus einereus. These strains showed gains and losses of restriction enzyme recognition sites as well as extensive insertion/ deletion variation within DNA sequences present in a single copy per haploi...

journal_title：Current genetics

authors： Wu MM,Cassidy JR,Pukkila PJ

更新日期：1983-09-01 00:00:00

abstract：:The cytoplasmic virus-like element pWR1A from Debaryomyces robertsiae encodes a toxin (DrT) with similarities to the Pichia acaciae killer toxin PaT, which acts by importing a toxin subunit (PaOrf2) with tRNA anticodon nuclease activity into target cells. As for PaT, loss of the tRNA methyltransferase Trm9 or overexpr...

journal_title：Current genetics

authors： Klassen R,Kast A,Wünsche G,Paluszynski JP,Wemhoff S,Meinhardt F

更新日期：2014-08-01 00:00:00

abstract：:The S. cerevisiae strain h56 is a temperature-sensitive mit- mutant containing a single nucleotide substitution in the region 5' to the reading frame of the mitochondrial var1 gene. The mutation decreases the efficiency of processing of a precursor RNA such that little var1 mRNA is produced at the restrictive temperat...

journal_title：Current genetics

authors： Smooker PM,Macreadie IJ,Wright JL,Lukins HB

更新日期：1994-03-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the HGS2-1 allele confers sensitivities to inorganis mercury (Ono and Sakamoto 1985) and to excess fermentable sugars such as glucose (Sakamoto et al. 1985); exogenous tyrosine antagonizes both inorganic mercury and excess glucose. In this study, the inorganic mercury sensitive strain has ...

journal_title：Current genetics

authors： Ono B,Sakamoto E,Yamaguchi K

更新日期：1987-01-01 00:00:00

abstract：:Similarity searches with Didymium iridis mitochondrial genomic DNA identified six possible ribosomal protein-coding regions, however, each region contained stop codons that would need to be removed by RNA editing to produce functional transcripts. RT-PCR was used to amplify these regions from total RNA for cloning and...

journal_title：Current genetics

authors： Hendrickson PG,Silliker ME

更新日期：2010-06-01 00:00:00

abstract：:In the photosynthetic chloroplast mutant PRB2A of Chlamydomonas reinhardtii the psbD mRNA is unstable. Three strains were isolated, in which the underlying site-directed mutation within the psbD 5' UTR (untranslated region) is suppressed. In all three suppressors, psbD RNA levels and RNA 5' maturation are restored to ...

journal_title：Current genetics

authors： Nickelsen J

更新日期：2000-02-01 00:00:00

abstract：:Although the mitochondrial genomes of the Chinese Spring and Aquila varieties of wheat are normally similar in organization, this is not so in tissue cultures initiated from their immature embryos where the mitochondrial genomes of both are rearranged and in different, characteristic, ways. However, the mitochondrial ...

journal_title：Current genetics

authors： Hartmann C,De Buyser J,Henry Y,Morère-Le Paven MC,Dyer TA,Rode A

更新日期：1992-05-01 00:00:00

abstract：:A gene encoding mitochondrial S13 is generally present in the mitochondrial genome of higher plants, but is lacking from the Arabidopsis thaliana mitochondrial genome. Previous research has failed to identify a nuclear gene capable of encoding a mitochondrial S13 protein or the protein itself. Doubts have even been ra...

journal_title：Current genetics

authors： Mollier P,Hoffmann B,Debast C,Small I

更新日期：2002-03-01 00:00:00

abstract：:In the filamentous ascomycete fungus Podospora anserina, senescence is associated with dramatic changes of the juvenile mitochondrial genome. During senescence specific regions of the non-senescent genome are excised, ligated and amplified as a plasmid. In this paper, we report our studies on nuclear-mitochondrial ext...

journal_title：Current genetics

authors： Steinhilber W,Cummings DJ

更新日期：1986-01-01 00:00:00

abstract：:A delta-9 fatty acid desaturase gene, homologous to animal and fungal acyl-coenzyme A (CoA) desaturases, was isolated from the red alga Cyanidioschyzon merolae using a degenerate PCR strategy. This gene, designated as CmFAD9, has no intron. The encoded delta-9 fatty acid desaturase (CmFad9p) consists of 476 amino acid...

journal_title：Current genetics

authors： Itoh R,Toda K,Takahashi H,Takano H,Kuroiwa T

更新日期：1998-03-01 00:00:00

abstract：:The circular mitochondrial genome of Dictyostelium discoideum has a size of 55,564 base pairs. We present here a complete and detailed transcription map of the mitochondrial DNA. Eight major, polycistronic transcripts encoding polypeptides, ribosomal RNAs and interspersed transfer RNAs were identified in Northern hybr...

journal_title：Current genetics

authors： Barth C,Greferath U,Kotsifas M,Tanaka Y,Alexander S,Alexander H,Fisher PR

更新日期：2001-07-01 00:00:00

abstract：:An Agrocybe aegerita cDNA library, constructed from fruit body primordia poly(A)+ RNAs, was screened by differential colony hybridization. Clones which preferentially hybridized to poly(A)+ RNA sequences from fruit body primordia, versus poly(A)+ RNAs from mycelium, were isolated. Eight of these clones (EMAa-1 to EMAa...

journal_title：Current genetics

authors： Salvado JC,Labarère J

更新日期：1991-08-01 00:00:00

abstract：:The Cdc42p GTPase regulates multiple signal transduction pathways through its interactions with downstream effectors. Specific functional domains within Cdc42p are required for guanine-nucleotide binding, interactions with downstream effectors, and membrane localization. However, little is known about how Cdc42p is cl...

journal_title：Current genetics

authors： Richman TJ,Toenjes KA,Morales SE,Cole KC,Wasserman BT,Taylor CM,Koster JA,Whelihan MF,Johnson DI

更新日期：2004-06-01 00:00:00

abstract：:The separation and identification of siderophores produced by microorganisms is a time-consuming and an expensive procedure. We have developed a new and efficient method to identify siderophores using well-established Saccharomyces cerevisiae deletion mutants. The Deltafet3,arn strains fail to sustain growth, even whe...

journal_title：Current genetics

authors： Park YS,Kim JH,Chang HI,Kim SW,Paik HD,Kang CW,Kim TH,Sung HC,Yun CW

更新日期：2007-09-01 00:00:00

abstract：:In the fungus Fusarium sporotrichioides, biosynthesis of trichothecene mycotoxins requires at least three genetic loci: a core 12-gene cluster, a smaller two-gene cluster, and a single-gene locus. Here, we describe the Tri15 gene, which represents a fourth locus involved in trichothecene biosynthesis. Tri15 is predict...

journal_title：Current genetics

authors： Alexander NJ,McCormick SP,Larson TM,Jurgenson JE

更新日期：2004-03-01 00:00:00

abstract：:Somatic fusion between cells of Chlamydomonas containing complementing cell wall and auxotrophic mutations and bearing chloroplast markers for resistance to antibiotics (streptomycin or spectinomycin) have been performed to analyze the mode of chloroplast gene transmission in the fusion products. Prototrophic colonies...

journal_title：Current genetics

authors： Matagne RF

更新日期：1981-04-01 00:00:00

abstract：:Mixed aeciospore isolates of Cronartium flaccidum and Peridermium pini were obtained from single-tree infections in Britain, Italy and Greece. The 5.8s ribosomal RNA gene and flanking intergenic transcribed spacer regions ITS1 and ITS2 were found to be highly similar between C. flaccidum and P. pini. Within samples he...

journal_title：Current genetics

authors： Moricca S,Kasuga T,Mitchelson K,Ragazzi A,Diamandis S

更新日期：1996-03-01 00:00:00

abstract：:Although cell division is usually portrayed as an equitable process by which a progenitor cell originates two identical daughter cells, there are multiple examples of asymmetric divisions that generate two cells that differ in their content, morphology and/or proliferative potential. The capacity of the cells to gener...

journal_title：Current genetics

authors： Manzano-López J,Monje-Casas F

更新日期：2020-08-01 00:00:00

abstract：:Analysis of the cDNA of the atp9-1 gene transcript from petunia mitochondria has revealed that ten C residues of the gene sequence are edited into U in the mRNA. Seven of these edits result in amino acid changes and one introduces a stop codon before the end of the open reading frame predicted from the gene sequence. ...

journal_title：Current genetics

authors： Wintz H,Hanson MR

更新日期：1991-01-01 00:00:00

abstract：:Fourteen nuclear complementation groups of mutants that specifically affect the three mitochondrially-encoded subunits of yeast cytochrome c oxidase have been characterized. Genes represented by these complementation groups are not required for mitochondrial transcription, transcript processing, or translation per se ...

journal_title：Current genetics

authors： Kloeckener-Gruissem B,McEwen JE,Poyton RO

更新日期：1987-01-01 00:00:00

abstract：:The chloroplast genome of black pine (Pinus thunbergii), a gymnosperm, contains 32 different tRNA genes, 30 of which correspond to those previously identified in tobacco and rice chloroplast genomes. Two additional genes encode tRNA(Pro) (GGG) and tRNA(Arg) (CCG); the former is newly identified while the latter is pre...

journal_title：Current genetics

authors： Tsudzuki J,Ito S,Tsudzuki T,Wakasugi T,Sugiura M

更新日期：1994-08-01 00:00:00

abstract：:Nucleic acid extracted from killer strains of Saccharomyces cerevisiae and run on agarose gels under sub-optimal conditions for M dsRNA yielded two species of L dsRNA (L1 and L2) which migrated at slightly different rates. L2 dsRNA was shown to be dependent upon MAK3, MAK10 and PES1 but not MAK1 nuclear genes for its ...

journal_title：Current genetics

authors： El-Sherbeini M,Bevan EA,Mitchell DJ

更新日期：1983-03-01 00:00:00

abstract：:An exceptional mode of mtDNA inheritance involving separate maternal and paternal transmission routes has been reported recently in the mussel Mytilus edulis. This mode of inheritance provides an explanation for the high levels of heteroplasmy for two highly diverged genomes observed in males of this species. Here we ...

journal_title：Current genetics

authors： Quesada H,Skibinski DA,Skibinski DO

更新日期：1996-04-01 00:00:00

abstract：:The unicellular green alga Chlamydomonas reinhardtii has emerged as a superb model species in plant biology. Although the alga is easily transformable, the low efficiency of transgene expression from the Chlamydomonas nuclear genome has severely hampered functional genomics research. For example, poor transgene expres...

journal_title：Current genetics

authors： Shao N,Bock R

更新日期：2008-06-01 00:00:00

abstract：:The nucleotide sequence of the ERG12 gene, encoding mevalonate kinase, from Saccharomyces cerevisiae is presented. The longest open reading frame may code for a protein containing 443 amino acids with a deduced relative molecular mass of 48,500. The analysis of the nucleotide sequence reveals a complete identity with ...

journal_title：Current genetics

authors： Oulmouden A,Karst F

更新日期：1991-01-01 00:00:00