Epitope-tagging vectors designed for yeast.

abstract：:A previously unknown nuclear gene DSS-1 from Saccharomyces cerevisiae was cloned and sequenced. The gene was isolated as a multicopy suppressor of a disruption of the SUV-3 gene coding for a DEAD/H box protein involved in processing and turnover of mitochondrial transcripts. The DSS-1 gene codes for a 970 amino-acid p...

journal_title：Current genetics

authors： Dmochowska A,Golik P,Stepien PP

更新日期：1995-07-01 00:00:00

abstract：:The influence of the DNA repair gene PSO3 on photoactivated psoralen-induced meiotic recombination, gene conversion, reverse mutation, and on survival, was assayed in diploid strains of Saccharomyces cerevisiae homozygous for the wild-type or the pso3-1 mutant allele. Sporulation was normal in the pso3-1 diploid. Wild...

journal_title：Current genetics

authors： Pothin HS,da Silva KV,Brendel M,Henriques JA

更新日期：1994-01-01 00:00:00

abstract：:The mother-bud neck is defined as the boundary between the mother cell and bud in budding microorganisms, wherein sequential morphological events occur throughout the cell cycle. This study was designed to quantitatively investigate the morphology of the mother-bud neck in budding yeast Saccharomyces cerevisiae. Obser...

journal_title：Current genetics

authors： Kubo K,Okada H,Shimamoto T,Kimori Y,Mizunuma M,Bi E,Ohnuki S,Ohya Y

更新日期：2019-02-01 00:00:00

abstract：:An exceptional mode of mtDNA inheritance involving separate maternal and paternal transmission routes has been reported recently in the mussel Mytilus edulis. This mode of inheritance provides an explanation for the high levels of heteroplasmy for two highly diverged genomes observed in males of this species. Here we ...

journal_title：Current genetics

authors： Quesada H,Skibinski DA,Skibinski DO

更新日期：1996-04-01 00:00:00

abstract：:Two plasmids containing Saccharomyces cerevisiae 2 µm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 µm DNA; in the presence of the LEU2 plasmids, 2 µm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 µm DNA loss has occurred, so that st...

journal_title：Current genetics

authors： Dobson MJ,Futcher AB,Cox BS

更新日期：1980-12-01 00:00:00

abstract：:It is now established that base-pairing regulatory RNAs are key players in post-transcriptional regulatory networks where they affect the translation and/or stability of their target RNAs. In many cases, the base-pairing between two RNAs is facilitated by an RNA-binding protein (RBP) that serves as an RNA chaperone. R...

journal_title：Current genetics

authors： Melamed S

更新日期：2020-08-01 00:00:00

abstract：:Cohesin is essential for sister chromatid cohesion, which ensures equal segregation of the chromatids to daughter cells. However, the molecular mechanism by which cohesin mediates this function is elusive. Scc3, one of the four core subunits of cohesin, is vital to cohesin activity. However, the mechanism by which Scc...

journal_title：Current genetics

authors： Pathania A,Liu W,Matityahu A,Irudayaraj J,Onn I

更新日期：2021-01-06 00:00:00

abstract：:In Aspergillus nidulans, carbon catabolite repression (CCR) is mediated by the global repressor protein CreA. The deubiquitinating enzyme CreB is a component of the CCR network. Genetic interaction was confirmed using a strain containing complete loss-of-function alleles of both creA and creB. No direct physical inter...

journal_title：Current genetics

authors： Alam MA,Kelly JM

更新日期：2017-08-01 00:00:00

abstract：:Most eukaryotes require molecular oxygen for growth. In general, oxygen is the terminal electron acceptor of the respiratory chain and represents an important substrate for the biosynthesis of cellular compounds. However, in their natural environment, such as soil, and also during the infection, filamentous fungi are ...

journal_title：Current genetics

authors： Hillmann F,Shekhova E,Kniemeyer O

更新日期：2015-08-01 00:00:00

abstract：:Sister chromatid cohesion is essential for chromosome segregation both in mitosis and meiosis. Cohesion between two chromatids is mediated by a protein complex called cohesin. The loading and unloading of the cohesin are tightly regulated during the cell cycle. In vertebrate cells, cohesin is released from chromosomes...

journal_title：Current genetics

authors： Challa K,Shinohara M,Shinohara A

更新日期：2019-08-01 00:00:00

abstract：:Mutations in the RecA-like genes RAD51 and RAD57 reduce the frequency of gene conversion/reciprocal exchange between inverted repeats 7-fold. However, they enhance the frequency of deletions between direct repeats 5-12-fold. These induced deletions are RAD1- and RAD52-dependent. On the basis of these results it is pro...

journal_title：Current genetics

authors： Aguilera A

更新日期：1995-03-01 00:00:00

abstract：:A molecular clone containing the complete sequence of a mitochondrial circular plasmid-like DNA (the R plasmid) isolated from the date-palm variety V3DP was used as a probe in Southern analyses of mitochondrial DNA prepared from other varieties. Another circular structure (the S plasmid) was detected in some of these ...

journal_title：Current genetics

authors： Benslimane AA,Hartmann C,Ouenzar B,Rode A

更新日期：1996-05-01 00:00:00

abstract：:In fungi, sexual reproduction primarily depends on the interaction between peptide pheromones and their receptors. Most ascomycete fungi produce two classes of peptide mating pheromones, a simple peptide and a modified peptide. These peptides are recognized by their corresponding receptors on the surface of cells of t...

journal_title：Current genetics

authors： Seike T

更新日期：2019-10-01 00:00:00

abstract：:We have used a PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), to assess genome variability between 21 isolates from F. solani f. sp. cucurbitae races 1 and 2. Based on RAPD marker patterns the isolates fell into two distinct groups corresponding to mating populations MPI and MPV. Fo...

journal_title：Current genetics

authors： Crowhurst RN,Hawthorne BT,Rikkerink EH,Templeton MD

更新日期：1991-11-01 00:00:00

abstract：:Tuplp is a general corepressor in Saccharomyces cerevisiae. We performed a split-ubiquitin screen with Tup1p as bait, in order to understand how Tuplp mediates repression. Cdc73p was the only component of a holoenzyme of transcription isolated as a Tuplp-interacting protein in this screen. Expression analysis of a str...

journal_title：Current genetics

authors： Kerkmann K,Lehming N

更新日期：2001-07-01 00:00:00

abstract：:Inorganic phosphate is an essential nutrient because it is required for the biosynthesis of nucleotides, phospholipids and metabolites in energy metabolism. During phosphate starvation, phosphatases play a major role in phosphate acquisition by hydrolyzing phosphorylated macromolecules. In Saccharomyces cerevisiae, PH...

journal_title：Current genetics

authors： Yadav KK,Singh N,Rajasekharan R

更新日期：2016-05-01 00:00:00

abstract：:Although cell division is usually portrayed as an equitable process by which a progenitor cell originates two identical daughter cells, there are multiple examples of asymmetric divisions that generate two cells that differ in their content, morphology and/or proliferative potential. The capacity of the cells to gener...

journal_title：Current genetics

authors： Manzano-López J,Monje-Casas F

更新日期：2020-08-01 00:00:00

abstract：:Forces generated by molecular motors and the cytoskeleton move the nucleus and genome during many cellular processes, including cell migration and division. How these forces impact the genome, and whether cells regulate cytoskeletal forces to preserve genome integrity is unclear. We recently demonstrated that, in budd...

journal_title：Current genetics

authors： Estrem C,Moore JK

更新日期：2020-04-01 00:00:00

abstract：:Chloroplast DNAs from six different laboratory collections of Euglena gracilis "strain Z" and var. bacillaris were analyzed with restriction endonucleases EcoRI and Bam HI. The most variable portion of the organelle genome is the region containing the ribosomal cistrons. Intraspecific differences occur in both ribosom...

journal_title：Current genetics

authors： Wurtz EA,Buetow DE

更新日期：1981-07-01 00:00:00

abstract：:Similarity searches with Didymium iridis mitochondrial genomic DNA identified six possible ribosomal protein-coding regions, however, each region contained stop codons that would need to be removed by RNA editing to produce functional transcripts. RT-PCR was used to amplify these regions from total RNA for cloning and...

journal_title：Current genetics

authors： Hendrickson PG,Silliker ME

更新日期：2010-06-01 00:00:00

abstract：:The nucleotide sequences of the plastidal psbD genes of Bumilleriopsis filiformis and Porphyridium purpureum (encoding the D2 protein of photosystem II) are reported in this paper. The Bumilleriopsis sequence clusters together with Porphyridium when a most parsimonious protein tree of D2 sequences is constructed. A co...

journal_title：Current genetics

authors： Scherer S,Lechner S,Böger P

更新日期：1993-11-01 00:00:00

abstract：:Six ultra violet (UV) mutageneses were performed on the spo76 UV-sensitive mutant of Sordaria macrospora. Spo76 shows an early centromere cleavage associated with an arrest at the first meiotic division and therefore does not form ascospores. Moreover, it exhibits altered pairing structure (synaptonemal complex), reve...

journal_title：Current genetics

authors： Huynh AD,Leblon G,Zickler D

更新日期：1986-01-01 00:00:00

abstract：:We report the nucleotide sequence of three tRNA genes from maize mitochondria. The genes are located in two BamHI fragments, 3.55 and 5.7 kb long, adjacent to the S2 sequence in the maize mitochondrial genome. On the 3.55 kb BamHI fragment, we have characterized a tRNA(Cys)(GCA) gene. A strong sequence homology of thi...

journal_title：Current genetics

authors： Wintz H,Grienenberger JM,Weil JH,Lonsdale DM

更新日期：1988-03-01 00:00:00

abstract：:An Agrocybe aegerita cDNA library, constructed from fruit body primordia poly(A)+ RNAs, was screened by differential colony hybridization. Clones which preferentially hybridized to poly(A)+ RNA sequences from fruit body primordia, versus poly(A)+ RNAs from mycelium, were isolated. Eight of these clones (EMAa-1 to EMAa...

journal_title：Current genetics

authors： Salvado JC,Labarère J

更新日期：1991-08-01 00:00:00

abstract：:We have cloned and sequenced human and bovine cDNAs for the beta subunit of the ATP synthase (ATP-syn beta), a nuclear DNA (nDNA) encoded oxidative phosphorylation (OXPHOS) gene. The two cDNAs were found to share 99% amino acid homology and 94% nucleotide homology. The evolutionary rate of ATPsyn beta was then compare...

journal_title：Current genetics

authors： Wallace DC,Ye JH,Neckelmann SN,Singh G,Webster KA,Greenberg BD

更新日期：1987-01-01 00:00:00

abstract：:The Saccharomyces cerevisiae open reading frame YDL202w has been characterised in the course of the EUROFAN yeast genome analysis program. Disruption of YDL202w causes a respiratory deficient phenotype accompanied by a loss of mitochondrial DNA. This phenotype is usually found in mutants defective in mitochondrial rep...

journal_title：Current genetics

authors： Bui DM,Jarosch E,Schweyen RJ

更新日期：1997-05-01 00:00:00

abstract：:The REC1 gene of U. maydis has an important but ill-defined role in DNA recombination and repair. We have examined its role in plasmid-chromosome recombination. Plasmid DNA was linearized at various locations with respect to the cloned U. maydis PYR3 gene and introduced into cells by transformation. Chromosomal integr...

journal_title：Current genetics

authors： Banks GR,Kanuga N,Ealing J,Spanos A,Holden DW

更新日期：1992-12-01 00:00:00

abstract：:The complete nucleotide sequence of one of three linear DNA plasmids (pRS64-2) from the plant pathogenic fungus Rhizoctonia solani was determined. The pRS64-2 DNA consisted of 2877 nucleotides. The nucleotide sequences of the middle 2.2-kb regions of the other two plasmids (pRS64-1 and pRS64-3) were also determined. C...

journal_title：Current genetics

authors： Katsura K,Suzuki F,Miyashita SI,Nishi T,Hirochika H,Hashiba T

更新日期：1997-12-01 00:00:00

abstract：:The unicellular green alga Chlamydomonas reinhardtii has emerged as a superb model species in plant biology. Although the alga is easily transformable, the low efficiency of transgene expression from the Chlamydomonas nuclear genome has severely hampered functional genomics research. For example, poor transgene expres...

journal_title：Current genetics

authors： Shao N,Bock R

更新日期：2008-06-01 00:00:00

abstract：:Cellulases belong to the large family of glycosyl hydrolases (GHs) and are produced by a variety of bacteria and fungi. These extracellular enzymes act as endoglucanases (EGs), cellobiohydrolases or beta-glucosidases. In this paper, we describe molecular screening for EGs from the GH family 12. Using three homologous ...

journal_title：Current genetics

authors： Goedegebuur F,Fowler T,Phillips J,van der Kley P,van Solingen P,Dankmeyer L,Power SD

更新日期：2002-05-01 00:00:00