Abstract：
:We have cloned and sequenced human and bovine cDNAs for the beta subunit of the ATP synthase (ATP-syn beta), a nuclear DNA (nDNA) encoded oxidative phosphorylation (OXPHOS) gene. The two cDNAs were found to share 99% amino acid homology and 94% nucleotide homology. The evolutionary rate of ATPsyn beta was then compared with that of two mitochondrial DNA (mtDNA) ATP synthase genes (ATPase 6 and 8), seven other mtDNA OXPHOS genes, and a number of nuclear genes. The synonymous substitution rate for ATPsyn beta proved to be 1.9 x 10(-9) substitutions per site per year (substitutions x site-1 x year-1) (SSY). This is less than 1/2 that of the average nDNA gene, 1/12 the rate of ATPase 6 and 8, and 1/17 the rate of the average mtDNA gene. The synonymous and replacement substitution rates were used to calculate a new parameter, the "selective constraint ratio". This revealed that even the most variable mtDNA protein was more constrained than the average nDNA protein. Thus, the high substitution mutation rate and strong selective constraints of mammalian mtDNA proteins suggest that mtDNA mutations may result in a disproportionately large number of human hereditary diseases of OXPHOS.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Wallace DC,Ye JH,Neckelmann SN,Singh G,Webster KA,Greenberg BDdoi
10.1007/BF00434661subject
Has Abstractpub_date
1987-01-01 00:00:00pages
81-90issue
2eissn
0172-8083issn
1432-0983journal_volume
12pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract：:A transformation system utilizing Agrobacterium tumefaciens was developed for targeted gene disruption in Monilinia fructicola, a fungal pathogen that causes brown rot disease of stone fruits. Transformation with a vector containing the neomycin phosphotransferase II (nptII) cassette flanked with 4 kb cutinase gene (M...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-006-0059-0
更新日期：2006-05-01 00:00:00
abstract：:DNA polymerases are evolved to extend the 3'-OH of a growing primer annealed to a template DNA substrate. Since replicative DNA polymerases have a limited role while replicating structurally distorted template, translesion DNA polymerases mostly from Y-family come to the rescue of stalled replication fork and maintain...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-018-0918-5
更新日期：2019-06-01 00:00:00
abstract：:The Cercospora nicotianae SOR1 gene is required for resistance to singlet oxygen-generating photosensitizers. SOR1 was characterized in the wild-type and in five photosensitizer-sensitive mutant strains which are complemented to photosensitizer resistance by transformation with SOR1. Sequence analysis determined that ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050423
更新日期：1999-01-01 00:00:00
abstract：:The A-pathway of development in the basidiomycete fungus Schizophyllum commune may be activated by either of two mating-type loci, A alpha and A beta. A alpha consists of two multiallelic genes, Y and Z. Y contains a putative homeodomain; Z contains a homeodomain-related region. Non-self combinations of Y and Z form h...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF02221577
更新日期：1996-01-01 00:00:00
abstract：:Saccharomyces cerevisiae adapts to oxidative, osmotic stress and nutrient deprivation through transcriptional changes, decreased proliferation, and entry into other developmental pathways such as pseudohyphal formation and sporulation. Inositol pyrophosphates are necessary for these cellular responses. Inositol pyroph...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-020-01078-8
更新日期：2020-10-01 00:00:00
abstract：:Chaperonin 60 (Cpn60) is a well-established marker protein for eukaryotic mitochondria and plastids. In order to determine whether the small double-membrane-bounded organelle posterior to the nucleus in the apicomplexan Cryptosporidium parvum is a mitochondrion, the Cpn60 gene of C. parvum sporozoites ( CpCpn60) was a...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-003-0432-1
更新日期：2003-11-01 00:00:00
abstract：:1. Nuclear mutants lacking cytochrome c l spectrum have been isolated. 2. The electron flow through the respiratory chain of the mutants is defect in the cytochrome bc 1-segment. 3. Complementation analysis has yielded two major complementation groups. 4. Tetrade analysis has established the two complementation groups...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00390343
更新日期：1982-12-01 00:00:00
abstract：:Mitochondrial DNA from etiolated seedling of male-fertile (F) and male-sterile (S) sunflower (Helianthus annuus L.) has been investigated for the first time in an isogenic couple HA89. 1. The mt-DNAs isolated from the S and F cytoplasms give a sharp and symmetrical band at 1.706 g/ml in an analytical CsCI gradient ult...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00420319
更新日期：1985-03-01 00:00:00
abstract：:Arenicin-1, a 21-mer antimicrobial peptide exerts significant broad-spectrum antimicrobial activity with membrane-active mechanisms. However, owing to multiple mechanisms of cell death, the antibacterial mechanism of arenicin-1 requires detailed analysis. In the present study, arenicin-1-treated bacteria underwent an ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-018-0855-3
更新日期：2019-02-01 00:00:00
abstract：:Radioactive messenger RNA (mRNA) was isolated from a Saccharomyces cerevisiae strain containing the L double stranded RNA (dsRNA) species. This mRNA was hybridised to LdsRNA isolated from the same strain. Analysis of the hybrid formed shows it to be of a similar size to the LdsRNA. It is concluded from this result tha...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00420496
更新日期：1981-12-01 00:00:00
abstract：:Mitotic homologous recombination (HR) is an important mechanism for the repair of double-strand breakS and errors occurring during DNA replication. It is likely that the recombinational repair of DNA lesions occurs preferentially by sister chromatid exchanges that have no genetic consequences. However, most geneticall...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-002-0346-3
更新日期：2003-01-01 00:00:00
abstract：:Nucleic acid extracted from killer strains of Saccharomyces cerevisiae and run on agarose gels under sub-optimal conditions for M dsRNA yielded two species of L dsRNA (L1 and L2) which migrated at slightly different rates. L2 dsRNA was shown to be dependent upon MAK3, MAK10 and PES1 but not MAK1 nuclear genes for its ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00365682
更新日期：1983-03-01 00:00:00
abstract：:Antimycin-resistant (AR) mutants of the yeast Kluyveromyces lactis, obtained either spontaneously or after manganese treatment, were isolated and genetically characterized. Most of the mutants obtained after manganese mutagenesis and two spontaneous mutants, tolerated high antimycin concentrations (more than 10 microg...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00384609
更新日期：1987-01-01 00:00:00
abstract：:Bands of dsRNA were detected in five out of seven isolates of the entomopathogenic fungus Metarhizium flavoviride. The identity of these bands was proven by RNase and S1-nuclease treatments. The transference of dsRNA between isolates (from CG291 to CG442) was successfully carried out through forced heterokaryons. Isog...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050477
更新日期：1999-08-01 00:00:00
abstract：:To supply abundant ribosomes, multiple copies of ribosomal RNA genes (rDNA) are conserved from bacterial to human cells. In eukaryotic genomes, clusters of tandemly repeated rDNA units are present, and their number is stably maintained. Due to high level of transcription of rRNA genes, the repetitive structure is pron...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-019-00956-0
更新日期：2019-08-01 00:00:00
abstract：:In the red alga Gracilaria verrucosa, the genes encoding the large and the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) are separated by a short spacer of less than 131 bp. Sequencing of PCR-amplified Rubisco spacers from a number of populations of G. verrucosa was performed to assess the...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00309601
更新日期：1991-05-01 00:00:00
abstract：:Protoplasts of a pAN7-1-transformed isolate of Cladosporium fulvum race 4, harbouring a tandem duplication of the vector, and an untransformed race 5 isolate were fused, without selection for the presence of vector sequences. Fusion products were allowed to haploidize spontaneously. The inheritance of pAN7-1 sequences...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00312643
更新日期：1993-05-01 00:00:00
abstract：:A fine restriction map of the linear mitochondrial DNA of Tetrahymena pyriformis strain ST is presented. 1. Based on agarose gel electrophoresis data together with limited nucleotide sequences available on some restriction fragments, we estimate the actual size of this genome to be about 55,000 base pairs. 2. Seven tR...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00434053
更新日期：1985-01-01 00:00:00
abstract：:We have determined the size, the restriction map and the gene order of the mitochondrial genome of the yeast Saccharomyces uvarum. Sequence analysis of the mitochondrial COXII gene confirmed the position of this yeast in the Saccharomyces cerevisiae-like group, near Saccharomyces cerevisiae and Saccharomyces douglasii...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050308
更新日期：1998-01-01 00:00:00
abstract：:The atpB and atpE genes encode beta and epsilon subunits, respectively, of chloroplast ATP synthase and are co-transcribed in the plant species so far studied. In tobacco, an atpB gene-specific probe hybridizes to 2.7- and 2.3-kb transcripts. In addition to these, a probe from the atpE coding region hybridizes also to...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00309558
更新日期：1994-09-01 00:00:00
abstract：:A Neurospora crassa cytoplasmic ribosomal protein gene, crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolate crp-5 genomic sequences. Computer analysis of the DNA sequences sho...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00310894
更新日期：1993-01-01 00:00:00
abstract：:Chloroplast DNAs from six different laboratory collections of Euglena gracilis "strain Z" and var. bacillaris were analyzed with restriction endonucleases EcoRI and Bam HI. The most variable portion of the organelle genome is the region containing the ribosomal cistrons. Intraspecific differences occur in both ribosom...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00429820
更新日期：1981-07-01 00:00:00
abstract：:In order to facilitate the process of epitope-tagging of yeast proteins, we have constructed two Saccharomyces cerevisiae-Escherichia coli shuttle vectors that allow fusion of a sequence encoding an epitope of the human c-myc protein at the 3' end of any gene. An example of the use of this technique is presented. ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00352019
更新日期：1993-02-01 00:00:00
abstract：:International efforts are underway that aim at determining the complete genome sequence of the social amoeba Dictyostelium discoideum. As strategy, a whole chromosome shotgun (WCS) approach was chosen and each of the six Dictyostelium chromosomes was assigned to project partners. The project is well advanced, chromoso...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-003-0416-1
更新日期：2003-11-01 00:00:00
abstract：:A strain dependent growth on lactate in the presence of antimycin A (AA) has been observed - the strain D261 can grow on lactate and AA, whereas in the strain K8/6C antimycin A prevents the utilization of lactate and the induction of LDH.Genetic analysis demonstrates that growth on lactate in the presence of AA segreg...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00433906
更新日期：1984-08-01 00:00:00
abstract：:The RNA polymerase II (Pol II) of Schizosaccharomyces pombe is composed of 12 subunits. Subunit Rpb3 has sequence homology with the N-terminal domain of the prokaryotic alpha subunit, which plays a key role in RNA polymerase assembly. Together with the Rpb2 (the beta homologue) and Rpb11 (the second alpha homologue) s...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940100203
更新日期：2001-06-01 00:00:00
abstract：:The amino acid sequences of two barley ribosomal proteins, termed HvL17-1 and HvL17-2, were decoded from green leaf cDNA clones. The N-terminal sequences of the derived barley proteins are 48% identical to the N-terminal amino acid sequence of protein YL17 from the large subunit of yeast cytoplasmic ribosomes. Via arc...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00309605
更新日期：1991-05-01 00:00:00
abstract：:Transforming DNAs form cointegrates in Aspergillus nidulans by homologous and non-homologous recombination as well as by end-to-end ligation of linear fragments. This process has been studied by means of a model in which the linkage of a marker gene to the origin of autonomous replication AMA1 was selected for. Recomb...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00310500
更新日期：1994-10-01 00:00:00
abstract：:The previous ovservation that in dividing sister cells of Schizosaccharomyces pombe only one of two parallel divisions can be accompanied by a switch of mating type, herein termed "Miyata's rule", has been confirmed in pedigrees of diploid cells heterozygous for the mat2-Pm-B102 allele. Moreover, this rule appears to ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00417817
更新日期：1984-04-01 00:00:00
abstract：:Degenerative processes in the filamentous fungus Podospora anserina are strongly correlated with the instability of the mitochondrial genome. Among the sources of instability is the mobile group-II intron COX1-i1, also called intron alpha, which encodes a protein with a reverse transcriptase activity. In this paper we...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050003
更新日期：2000-01-01 00:00:00
