ALG/alg: a single gene controlling the utilization of lactate in the presence of antimycin in the yeast Saccharomyces cerevisiae.

abstract：:Chloroplast DNA from the fern Osmunda einnamomea was isolated by a sucrose gradient procedure utilizing PEG to stabilize chloroplasts. Analysis with the restriction endonucleases PvuII, Sacl and BstEII indicates a chloroplast genome size of 144 kb. A physical map of the fragments produced by these three enzymes was co...

journal_title：Current genetics

authors： Palmer JD,Stein DB

更新日期：1982-08-01 00:00:00

abstract：:Fission yeast is a simple eukaryotic model organism in which many aspects of cell cycle control can be explored. We examined by homologous recombination whether the human CDC25A phosphatase could substitute for the function of the fission yeast Cdc25. We first show: (a). that CDC25A efficiently replaces the endogenous...

journal_title：Current genetics

authors： Mondesert O,Lemaire M,Brezak MC,Galera-Contour MO,Prevost G,Ducommun B,Bugler B

更新日期：2004-05-01 00:00:00

abstract：:Genetic and molecular analyses of the phenomenon of senescence-i.e., irreversible loss of growth and reproductive potential upon subculturing-in Neurospora intermedia strain M1991-60A, collected from Maddur in southern India, showed the presence of plasmid pMaddur1, which is homologous to the senescence-inducing circu...

journal_title：Current genetics

authors： D'Souza AD,Sultana S,Maheshwari R

更新日期：2005-03-01 00:00:00

abstract：:In order to facilitate the process of epitope-tagging of yeast proteins, we have constructed two Saccharomyces cerevisiae-Escherichia coli shuttle vectors that allow fusion of a sequence encoding an epitope of the human c-myc protein at the 3' end of any gene. An example of the use of this technique is presented. ...

journal_title：Current genetics

authors： Reisdorf P,Maarse AC,Daignan-Fornier B

更新日期：1993-02-01 00:00:00

abstract：:Two different cytoplasmic male-sterility (CMS) systems, nap and pol, are found in the oilseed rape (canola) species Brassica napus. Physical mapping studies have previously shown that organizational differences between the sterile pol and fertile cam mitochondrial genomes are restricted to a relatively small region im...

journal_title：Current genetics

authors： L'Homme Y,Stahl RJ,Li XQ,Hameed A,Brown GG

更新日期：1997-04-01 00:00:00

abstract：:The A-pathway of development in the basidiomycete fungus Schizophyllum commune may be activated by either of two mating-type loci, A alpha and A beta. A alpha consists of two multiallelic genes, Y and Z. Y contains a putative homeodomain; Z contains a homeodomain-related region. Non-self combinations of Y and Z form h...

journal_title：Current genetics

authors： Shen GP,Park DC,Ullrich RC,Novotny CP

更新日期：1996-01-01 00:00:00

abstract：:Sequences hybridizing to mitochondrial DNA probes from Saccharomyces cerevisiae have been mapped in six mitochondrial genomes from the Dekkera/Brettanomyces yeasts and in mtDNA from the closely related Eeniella nana. Sequence order for the 34.5 kbp mtDNA of E. nana is identical to that for mtDNAs from B. custersianus ...

journal_title：Current genetics

authors： Hoeben P,Clark-Walker GD

更新日期：1986-01-01 00:00:00

abstract：:Cyh3 and cyh4, multiple drug resistant strains of Schizosaccharomyces pombe, show a much reduced uptake of trichodermin, chloramphenicol, cycloheximide, L-lysine, glycine, L-threonine, L-glutamine, L-arginine and L-glutamic acid when compared to wild type. The plasma membrane and mitochondrial ATPase activities of the...

journal_title：Current genetics

authors： Johnston PA,Coddington A

更新日期：1983-07-01 00:00:00

abstract：:Full genome recoding, or rewriting codon meaning, through chemical synthesis of entire bacterial chromosomes has become feasible in the past several years. Recoding an organism can impart new properties including non-natural amino acid incorporation, virus resistance, and biocontainment. The estimated cost of construc...

journal_title：Current genetics

authors： Kuo J,Stirling F,Lau YH,Shulgina Y,Way JC,Silver PA

更新日期：2018-04-01 00:00:00

abstract：:In order to analyse gene expression during fruit body development of the ectomychorrizal fungus Tuber borchii Vittad., a modified differential display procedure was set up. The procedure used is easier and faster than the traditional one and generates reproducible cDNA banding patterns that can be resolved on a standa...

journal_title：Current genetics

authors： Zeppa S,Guidi C,Zambonelli A,Potenza L,Vallorani L,Pierleoni R,Sacconi C,Stocchi V

更新日期：2002-12-01 00:00:00

abstract：:Mitotic homologous recombination (HR) is an important mechanism for the repair of double-strand breakS and errors occurring during DNA replication. It is likely that the recombinational repair of DNA lesions occurs preferentially by sister chromatid exchanges that have no genetic consequences. However, most geneticall...

journal_title：Current genetics

authors： Prado F,Cortés-Ledesma F,Huertas P,Aguilera A

更新日期：2003-01-01 00:00:00

abstract：:The cloning of fga1, the gene encoding a G protein alpha subunit, was performed by standard PCR techniques and by screening a Fusarium oxysporum genomic library, using the PCR product as a probe. The full-length open reading frame spanned 1,059 nucleotides and the deduced primary structure of the protein (353 amino ac...

journal_title：Current genetics

authors： Jain S,Akiyama K,Mae K,Ohguchi T,Takata R

更新日期：2002-09-01 00:00:00

abstract：:The cellulase gene cel3 has been isolated from Agaricus bisporus and sequenced. The 5'-end of the cel3 transcript was determined by primer extension and S1 nuclease protection. Putative regulatory elements have been identified in the cel3 promoter and 3'-untranslated regions. The cel3 coding region is interrupted by s...

journal_title：Current genetics

authors： Yagüe E,Chow CM,Challen MP,Thurston CF

更新日期：1996-06-01 00:00:00

abstract：:An Agrocybe aegerita cDNA library, constructed from fruit body primordia poly(A)+ RNAs, was screened by differential colony hybridization. Clones which preferentially hybridized to poly(A)+ RNA sequences from fruit body primordia, versus poly(A)+ RNAs from mycelium, were isolated. Eight of these clones (EMAa-1 to EMAa...

journal_title：Current genetics

authors： Salvado JC,Labarère J

更新日期：1991-08-01 00:00:00

abstract：:Cells divide with appropriate frequency by coupling division to growth-that is, cells divide only when they have grown sufficiently large. This process is poorly understood, but has been studied using cell size mutants. In principle, mutations affecting cell size could affect the mean size ("set-point" mutants), or th...

journal_title：Current genetics

authors： Chen Y,Futcher B

更新日期：2020-11-05 00:00:00

abstract：:A transformation system utilizing Agrobacterium tumefaciens was developed for targeted gene disruption in Monilinia fructicola, a fungal pathogen that causes brown rot disease of stone fruits. Transformation with a vector containing the neomycin phosphotransferase II (nptII) cassette flanked with 4 kb cutinase gene (M...

journal_title：Current genetics

authors： Lee MH,Bostock RM

更新日期：2006-05-01 00:00:00

abstract：:The cytoplasmic virus-like element pWR1A from Debaryomyces robertsiae encodes a toxin (DrT) with similarities to the Pichia acaciae killer toxin PaT, which acts by importing a toxin subunit (PaOrf2) with tRNA anticodon nuclease activity into target cells. As for PaT, loss of the tRNA methyltransferase Trm9 or overexpr...

journal_title：Current genetics

authors： Klassen R,Kast A,Wünsche G,Paluszynski JP,Wemhoff S,Meinhardt F

更新日期：2014-08-01 00:00:00

abstract：:In this work, we explore the use of the unbiased cDNA-AFLP strategy to identify genes involved in Mn(2+) homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been sequenced, three Mn peroxidase genes responding to Mn(2+) have been characterized. Using cDNA-AFLP to...

journal_title：Current genetics

authors： Gutiérrez M,Rojas LA,Mancilla-Villalobos R,Seelenfreund D,Vicuña R,Lobos S

更新日期：2008-10-01 00:00:00

abstract：:The Cys6Zn2 DNA-binding domain transcription factor Pdr1 is a central regulator of drug resistance in the pathogenic yeast Candida glabrata. In this review, I discuss the multiple control mechanisms modulating the function of this positive transcriptional regulator. Available data suggest that Pdr1 activity is restrai...

journal_title：Current genetics

authors： Moye-Rowley WS

更新日期：2019-02-01 00:00:00

abstract：:As part of developing an ex planta model system for the study of maize plastid and mitochondrial gene expression, a series of established Black Mexican Sweet (BMS) suspension cell lines was characterized. Although the initial assumption was that their organelle biochemistry would be similar enough to normal in planta ...

journal_title：Current genetics

authors： Cahoon AB,Cunningham KA,Bollenbach TJ,Stern DB

更新日期：2003-11-01 00:00:00

abstract：:A previously unknown nuclear gene DSS-1 from Saccharomyces cerevisiae was cloned and sequenced. The gene was isolated as a multicopy suppressor of a disruption of the SUV-3 gene coding for a DEAD/H box protein involved in processing and turnover of mitochondrial transcripts. The DSS-1 gene codes for a 970 amino-acid p...

journal_title：Current genetics

authors： Dmochowska A,Golik P,Stepien PP

更新日期：1995-07-01 00:00:00

abstract：:The chloroplast genome of black pine (Pinus thunbergii), a gymnosperm, contains 32 different tRNA genes, 30 of which correspond to those previously identified in tobacco and rice chloroplast genomes. Two additional genes encode tRNA(Pro) (GGG) and tRNA(Arg) (CCG); the former is newly identified while the latter is pre...

journal_title：Current genetics

authors： Tsudzuki J,Ito S,Tsudzuki T,Wakasugi T,Sugiura M

更新日期：1994-08-01 00:00:00

abstract：:The zinc finger transcription factor Crz1 is an important downstream regulator of calcium-dependent signal transduction pathways in many organisms. The function of Crz1 in the wheat-head blight pathogen Fusarium graminearum remains unclear. In this study, we identified and functionally characterised FgCrz1A, a potenti...

journal_title：Current genetics

authors： Chen L,Tong Q,Zhang C,Ding K

更新日期：2019-02-01 00:00:00

abstract：:To understand the organization of the mating type locus of Stagonosporopsis tanaceti and Stagonosporopsis chrysanthemi, and its potential role in the epidemiology of ray blight of pyrethrum and chrysanthemum, respectively, the mating type (MAT) locus of these species was cloned and characterized using PCR-based techni...

journal_title：Current genetics

authors： Chilvers MI,Jones S,Meleca J,Peever TL,Pethybridge SJ,Hay FS

更新日期：2014-11-01 00:00:00

abstract：:The endpoints of an inversion in wheat chloroplast DNA are shown to be associated with copies of a short repeated sequence. Recombination across the repeats in an inverted configuration may have been responsible for the inversion, although they are currently in a direct orientation owing to a second inversion. The rep...

journal_title：Current genetics

authors： Howe CJ

更新日期：1985-01-01 00:00:00

abstract：:We have analysed two new genes, YBR1007 and YBR1015, discovered during the systematic sequencing of chromosome II of S. cerevisiae. YBR1007 shows strong similarities to amino-acid permeases, in particular the high-affinity proline permeases of S. cerevisiae and A. nidulans. The number and position of the predicted mem...

journal_title：Current genetics

authors： Nasr F,Bécam AM,Grzybowska E,Zagulski M,Slonimski PP,Herbert CJ

更新日期：1994-07-01 00:00:00

abstract：:The nucleotide sequences of regions containing the mating-type locus of the plant-pathogenic ascomycete Leptosphaeria maculans are described. The MAT1-1 gene is 1,368 bp, encoding a predicted protein of 441 amino acids, with a 45-bp intron. The MAT1-2 gene is 1,246 bp, encoding a predicted protein of 397 amino acids, ...

journal_title：Current genetics

authors： Cozijnsen AJ,Howlett BJ

更新日期：2003-08-01 00:00:00

abstract：:The bacterial hexameric helicase known as Rho is an archetypal sequence-specific transcription terminator that typically halts the synthesis of a defined set of transcripts, particularly those bearing cytosine-rich 3'-untranslated regions. However, under conditions of translational stress, Rho can also terminate trans...

journal_title：Current genetics

authors： Lawson MR,Berger JM

更新日期：2019-06-01 00:00:00

abstract：:Cell fusion occurs regularly during the vegetative and sexual phases of the life cycle in filamentous fungi. Here, we present a simple and efficient method that can detect even rare hyphal fusion events. Using the homothallic ascomycete Sordaria macrospora as an experimental system, we developed a histone-assisted mer...

journal_title：Current genetics

authors： Rech C,Engh I,Kück U

更新日期：2007-11-01 00:00:00

abstract：:In the fungus Fusarium sporotrichioides, biosynthesis of trichothecene mycotoxins requires at least three genetic loci: a core 12-gene cluster, a smaller two-gene cluster, and a single-gene locus. Here, we describe the Tri15 gene, which represents a fourth locus involved in trichothecene biosynthesis. Tri15 is predict...

journal_title：Current genetics

authors： Alexander NJ,McCormick SP,Larson TM,Jurgenson JE

更新日期：2004-03-01 00:00:00