Detection of hyphal fusion in filamentous fungi using differently fluorescence-labeled histones.

abstract：:The separation and identification of siderophores produced by microorganisms is a time-consuming and an expensive procedure. We have developed a new and efficient method to identify siderophores using well-established Saccharomyces cerevisiae deletion mutants. The Deltafet3,arn strains fail to sustain growth, even whe...

journal_title：Current genetics

authors： Park YS,Kim JH,Chang HI,Kim SW,Paik HD,Kang CW,Kim TH,Sung HC,Yun CW

更新日期：2007-09-01 00:00:00

abstract：:In order to facilitate the process of epitope-tagging of yeast proteins, we have constructed two Saccharomyces cerevisiae-Escherichia coli shuttle vectors that allow fusion of a sequence encoding an epitope of the human c-myc protein at the 3' end of any gene. An example of the use of this technique is presented. ...

journal_title：Current genetics

authors： Reisdorf P,Maarse AC,Daignan-Fornier B

更新日期：1993-02-01 00:00:00

abstract：:A PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), was used for assessing genomic variability among a wide range of culture collection strains of black Aspergilli and related species. The performance of this technique is compared with that of the two other genetic techniques most comm...

journal_title：Current genetics

authors： Megnegneau B,Debets F,Hoekstra RF

更新日期：1993-01-01 00:00:00

abstract：:The mitochondrial genomes are one of the most information-rich markers in phylogenetics. The relationships within superorder Dictyoptera have been debated in the literature. However, the closely related termites (Isoptera) are retained as unranked taxon within the order Blattaria (cockroaches). In this work, we sequen...

journal_title：Current genetics

authors： Xiao B,Chen AH,Zhang YY,Jiang GF,Hu CC,Zhu CD

更新日期：2012-04-01 00:00:00

abstract：:The RAD50 gene in yeast is required for recombination-repair (i.e., the double strand break repair pathway) in mitosis, and for meiotic recombination and sporulation. Both of these processes are complex and seem likely to require a relatively large number of gene products. In order to help define other genes required ...

journal_title：Current genetics

authors： Malone RE,Jordan K,Wardman W

更新日期：1985-01-01 00:00:00

abstract：:In the filamentous ascomycete fungus Podospora anserina, senescence is associated with dramatic changes of the juvenile mitochondrial genome. During senescence specific regions of the non-senescent genome are excised, ligated and amplified as a plasmid. In this paper, we report our studies on nuclear-mitochondrial ext...

journal_title：Current genetics

authors： Steinhilber W,Cummings DJ

更新日期：1986-01-01 00:00:00

abstract：:In the photosynthetic chloroplast mutant PRB2A of Chlamydomonas reinhardtii the psbD mRNA is unstable. Three strains were isolated, in which the underlying site-directed mutation within the psbD 5' UTR (untranslated region) is suppressed. In all three suppressors, psbD RNA levels and RNA 5' maturation are restored to ...

journal_title：Current genetics

authors： Nickelsen J

更新日期：2000-02-01 00:00:00

abstract：:The A-pathway of development in the basidiomycete fungus Schizophyllum commune may be activated by either of two mating-type loci, A alpha and A beta. A alpha consists of two multiallelic genes, Y and Z. Y contains a putative homeodomain; Z contains a homeodomain-related region. Non-self combinations of Y and Z form h...

journal_title：Current genetics

authors： Shen GP,Park DC,Ullrich RC,Novotny CP

更新日期：1996-01-01 00:00:00

abstract：:To supply abundant ribosomes, multiple copies of ribosomal RNA genes (rDNA) are conserved from bacterial to human cells. In eukaryotic genomes, clusters of tandemly repeated rDNA units are present, and their number is stably maintained. Due to high level of transcription of rRNA genes, the repetitive structure is pron...

journal_title：Current genetics

authors： Iida T,Kobayashi T

更新日期：2019-08-01 00:00:00

abstract：:Many species are able to hybridize, but the sterility of these hybrids effectively prevents gene flow between the species, reproductively isolating them and allowing them to evolve independently. Yeast hybrids formed by Saccharomyces cerevisiae and Saccharomyces paradoxus parents are viable and able to grow by mitosis...

journal_title：Current genetics

authors： Ono J,Greig D

更新日期：2020-06-01 00:00:00

abstract：:In yeast (S. cerevisiae) the stringent response is known to include rapid, selective, and severe transcriptional curtailment for genes specifying cytoplasmic rRNAs and r-proteins. We have shown that transcription of the mitochondrial 21S rRNA gene is also congruently and selectively curtailed during the yeast stringen...

journal_title：Current genetics

authors： Cantwell R,McEntee CM,Hudson AP

更新日期：1992-03-01 00:00:00

abstract：:Wild-type Neurospora crassa, strain Singapore, was transformed with a N. crassa metallothionein promoter/protyrosinase fusion gene. Transformants produced tyrosinase during vegetative growth, as determined by Western analyses and activity assays. This is in sharp contrast to wild-type strains, where this enzyme is onl...

journal_title：Current genetics

authors： Kupper U,Linden M,Cao KZ,Lerch K

更新日期：1990-11-01 00:00:00

abstract：:Bands of dsRNA were detected in five out of seven isolates of the entomopathogenic fungus Metarhizium flavoviride. The identity of these bands was proven by RNase and S1-nuclease treatments. The transference of dsRNA between isolates (from CG291 to CG442) was successfully carried out through forced heterokaryons. Isog...

journal_title：Current genetics

authors： Martins MK,Furlaneto MC,Sosa-Gomez DR,Faria MR,Fungaro MH

更新日期：1999-08-01 00:00:00

abstract：:The previous ovservation that in dividing sister cells of Schizosaccharomyces pombe only one of two parallel divisions can be accompanied by a switch of mating type, herein termed "Miyata's rule", has been confirmed in pedigrees of diploid cells heterozygous for the mat2-Pm-B102 allele. Moreover, this rule appears to ...

journal_title：Current genetics

authors： Egel R

更新日期：1984-04-01 00:00:00

abstract：:The soil-borne, asexual fungus Fusarium oxysporum f.sp. lycopersici (Fol) is a causal agent of tomato wilt disease. The infection process of Fol comprises root recognition, adhesion, penetration, colonization of the root cortex and hyphal proliferation within the xylem vessels, which are under the regulation of virule...

journal_title：Current genetics

authors： Yun Y,Zhou X,Yang S,Wen Y,You H,Zheng Y,Norvienyeku J,Shim WB,Wang Z

更新日期：2019-06-01 00:00:00

abstract：:In fungi, sexual reproduction primarily depends on the interaction between peptide pheromones and their receptors. Most ascomycete fungi produce two classes of peptide mating pheromones, a simple peptide and a modified peptide. These peptides are recognized by their corresponding receptors on the surface of cells of t...

journal_title：Current genetics

authors： Seike T

更新日期：2019-10-01 00:00:00

abstract：:Two types of DNA sequence polymorphisms were found among different geographic isolates of the basidiomycete fungus Coprinus einereus. These strains showed gains and losses of restriction enzyme recognition sites as well as extensive insertion/ deletion variation within DNA sequences present in a single copy per haploi...

journal_title：Current genetics

authors： Wu MM,Cassidy JR,Pukkila PJ

更新日期：1983-09-01 00:00:00

abstract：:Ime1 plays a pivotal role in the initiation of meiosis in a/alpha diploid cells of Saccharomyces cerevisiae. In the absence of glucose and nitrogen, IME1 expression is greater in a/alpha cells than in either a or alpha cells and therefore only a/alpha, but not a/a or alpha/alpha, cells are committed to sporulation. It...

journal_title：Current genetics

authors： Mizuno T,Nakazawa N,Remgsamrarn P,Kunoh T,Oshima Y,Harashima S

更新日期：1998-04-01 00:00:00

abstract：:Exonuclease II (ExoII) from Schizosaccharomyces pombe is a 5'-->3' single-stranded DNA exonuclease. We have cloned its gene, exo2, whose nucleotide sequence revealed that ExoII is a homologue of the multifunctional Saccharomyces cerevisiae Sep1 protein (also called Kem1, Xrn1, Rar5, Dst2). S. pombe exo2 null mutants w...

journal_title：Current genetics

authors： Szankasi P,Smith GR

更新日期：1996-09-01 00:00:00

abstract：:The atpB and atpE genes encode beta and epsilon subunits, respectively, of chloroplast ATP synthase and are co-transcribed in the plant species so far studied. In tobacco, an atpB gene-specific probe hybridizes to 2.7- and 2.3-kb transcripts. In addition to these, a probe from the atpE coding region hybridizes also to...

journal_title：Current genetics

authors： Kapoor S,Wakasugi T,Deno H,Sugiura M

更新日期：1994-09-01 00:00:00

abstract：:The circular mitochondrial genome of Dictyostelium discoideum has a size of 55,564 base pairs. We present here a complete and detailed transcription map of the mitochondrial DNA. Eight major, polycistronic transcripts encoding polypeptides, ribosomal RNAs and interspersed transfer RNAs were identified in Northern hybr...

journal_title：Current genetics

authors： Barth C,Greferath U,Kotsifas M,Tanaka Y,Alexander S,Alexander H,Fisher PR

更新日期：2001-07-01 00:00:00

abstract：:Cellulases belong to the large family of glycosyl hydrolases (GHs) and are produced by a variety of bacteria and fungi. These extracellular enzymes act as endoglucanases (EGs), cellobiohydrolases or beta-glucosidases. In this paper, we describe molecular screening for EGs from the GH family 12. Using three homologous ...

journal_title：Current genetics

authors： Goedegebuur F,Fowler T,Phillips J,van der Kley P,van Solingen P,Dankmeyer L,Power SD

更新日期：2002-05-01 00:00:00

abstract：:Sexual reproduction in Agrocybe aegerita is controlled by the tetrapolar mechanism of homogenic incompatibility. The two mating factors, A and B, are nonequivalent and exhibit functional specificity. Fourteen distinct A factors and 17 distinct B factors were identified in 17 natural isolates from Europe and Japan. ...

journal_title：Current genetics

authors： Meinhardt F,Leslie JF

更新日期：1982-05-01 00:00:00

abstract：:A Neurospora crassa cytoplasmic ribosomal protein gene, crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolate crp-5 genomic sequences. Computer analysis of the DNA sequences sho...

journal_title：Current genetics

authors： Wang Z,Tarawneh KA,Free SJ

更新日期：1993-01-01 00:00:00

abstract：:The isoprenoid pathway of the ectomycorrhizal fungus Tuber borchii Vittad is investigated to better understand the molecular mechanisms at work, in particular during the maturation of the complex ascomata (the so-called "truffles"). Three T. borchii genes coding for the most important regulatory enzymes of the isopren...

journal_title：Current genetics

authors： Guidi C,Zeppa S,Annibalini G,Pierleoni R,Guescini M,Buffalini M,Zambonelli A,Stocchi V

更新日期：2006-12-01 00:00:00

abstract：:RNA/DNA hybrids are processed by RNases H1 and H2, while single ribonucleoside-monophosphates (rNMPs) embedded in genomic DNA are removed by the error-free, RNase H2-dependent ribonucleotide excision repair (RER) pathway. In the absence of RER, however, topoisomerase 1 (Top1) can cleave single genomic rNMPs in a mutag...

journal_title：Current genetics

authors： Cerritelli SM,El Hage A

更新日期：2020-12-01 00:00:00

abstract：:In Chlamydomonas reinhardtii, the expression of the nit1 gene encoding nitrate reductase is dependent on the nature of the nitrogen source and on other environmental factors. We have fused the nit1 promoter region to the arylsulphatase (ars) reporter gene lacking its own promoter and introduced this chimeric construct...

journal_title：Current genetics

authors： Ohresser M,Matagne RF,Loppes R

更新日期：1997-03-01 00:00:00

abstract：:The Saccharomyces cerevisiae nuclear gene MRP-S9 was identified as part of the European effort in sequencing chromosome II. MRP-S9 encodes for a hydrophilic and basic protein of 278 amino acids with a molecular mass of 32 kDa. The C-terminal part (aa 153-278) of the MRP-S9 protein exhibits significant sequence similar...

journal_title：Current genetics

authors： Kötter P,Entian KD

更新日期：1995-06-01 00:00:00

abstract：:Gene expression using alternative transcription start sites (TSSs) is an important transcriptional regulatory mechanism for environmental responses in eukaryotes. Here, we identify two alternative TSSs in the enolase-encoding gene (enoA) in Aspergillus oryzae, an industrially important filamentous fungus. TSS use in e...

journal_title：Current genetics

authors： Inoue T,Toji H,Tanaka M,Takama M,Hasegawa-Shiro S,Yamaki Y,Shintani T,Gomi K

更新日期：2020-08-01 00:00:00

abstract：:Botryotinia fuckeliana, the causal agent of grey mould, was biolistically transformed to hygromycin B resistance using a plasmid (pOHT) containing a bacterial hygromycin phosphotransferase gene fused to regulatory sequences from Aspergillus nidulans. Multiple copies of the plasmid, precipitated onto tungsten particles...

journal_title：Current genetics

authors： Hilber UW,Bodmer M,Smith FD,Köller W

更新日期：1994-02-01 00:00:00