Abstract:
:Wild-type Neurospora crassa, strain Singapore, was transformed with a N. crassa metallothionein promoter/protyrosinase fusion gene. Transformants produced tyrosinase during vegetative growth, as determined by Western analyses and activity assays. This is in sharp contrast to wild-type strains, where this enzyme is only expressed in situations of starvation or sexual differentiation. Complete integration of a 400 bp metallothionein promoter-fragment leads to constitutive expression of protyrosinase, whereas a 3.6 kb promoter-fragment conferred copper inducibility on the reporter gene in four transformants. A transformant with high constitutive tyrosinase levels was able to produce melanin on complete medium agar plates supplemented with 1 mg/ml L-tyrosine.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Kupper U,Linden M,Cao KZ,Lerch Kdoi
10.1007/BF00318214subject
Has Abstractpub_date
1990-11-01 00:00:00pages
331-5issue
4eissn
0172-8083issn
1432-0983journal_volume
18pub_type
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