A Saccharomyces paradox: chromosomes from different species are incompatible because of anti-recombination, not because of differences in number or arrangement.

abstract：:In order to study pathogenic mechanisms of the plant pathogen Ascochyta rabiei, conditions for efficient transformation using Agrobacterium-mediated transformation were investigated. Hygromycin B resistance (hph) was superior to geneticin resistance (nptII) for selecting transformants, and the hph gene was more effici...

journal_title：Current genetics

authors： White D,Chen W

更新日期：2006-04-01 00:00:00

abstract：:Mitochondrial DNA from etiolated seedling of male-fertile (F) and male-sterile (S) sunflower (Helianthus annuus L.) has been investigated for the first time in an isogenic couple HA89. 1. The mt-DNAs isolated from the S and F cytoplasms give a sharp and symmetrical band at 1.706 g/ml in an analytical CsCI gradient ult...

journal_title：Current genetics

authors： Leroy P,Bazetoux S,Quétier F,Delbut J,Bervillé A

更新日期：1985-03-01 00:00:00

abstract：:Mono-ubiquitinated histone H2B (H2B-Ub) is important for chromatin regulation of transcription, chromatin assembly, and also influences heterochromatin. In this review, we discuss the effects of H2B-Ub from nucleosome to higher-order chromatin structure. We then assess what is currently known of the role of H2B-Ub in ...

journal_title：Current genetics

authors： Zukowski A,Johnson AM

更新日期：2018-08-01 00:00:00

abstract：:Adaptation by natural selection might improve the fitness of an organism and its probability to survive in unfavorable environmental conditions. Decoding the genetic basis of adaptive evolution is one of the great challenges to deal with. To this purpose, Saccharomyces cerevisiae has been largely investigated because ...

journal_title：Current genetics

authors： Tosato V,Sims J,West N,Colombin M,Bruschi CV

更新日期：2017-05-01 00:00:00

abstract：:We find three interesting characteristics of the genes encoding the ribosomal RNA (rRNA) in the basidiomycete Coprinus cinereus. First, there are only 60 to 90 copies of the genes, fewer than in other fungi. Second, the genes are organized in an unusual arrangement. The 5S rRNA genes are located in the repeat unit whi...

journal_title：Current genetics

authors： Rhodes Cassidy J,Moore D,Lu BC,Pukkila PJ

更新日期：1984-10-01 00:00:00

abstract：:1. Nuclear mutants lacking cytochrome c l spectrum have been isolated. 2. The electron flow through the respiratory chain of the mutants is defect in the cytochrome bc 1-segment. 3. Complementation analysis has yielded two major complementation groups. 4. Tetrade analysis has established the two complementation groups...

journal_title：Current genetics

authors： Lang BF,Kaudewitz F

更新日期：1982-12-01 00:00:00

abstract：:In Chlamydomonas reinhardtii, one displacement loop region which initiates the replication of chloroplast DNA was located on a 1.05 kb restriction fragment. This fragment was cloned and sequenced. In this report, the galK expression plasmid, pKO1 was used to screen for the presence of any prokaryotic promoter within t...

journal_title：Current genetics

authors： Wu M,Kong XF,Kung SD

更新日期：1986-01-01 00:00:00

abstract：:The meiotic recombination gene rec10, which encodes a region-specific activator of recombination in Schizosaccharomyces pombe, has been cloned by genetic complementation and its nucleotide sequence determined. The rec10 gene was identified in a 5.6-kb cloned fragment by partial-deletion and insertion experiments. The ...

journal_title：Current genetics

authors： Lin Y,Smith GR

更新日期：1995-04-01 00:00:00

abstract：:The GGP1 gene encodes the only GPI-anchored glycoprotein (gp115) that has been purified to-date in the budding yeast Saccharomyces cerevisiae. It is a single-copy gene whose deduced amino-acid sequence shares no significant homology to any other known protein. In this paper we report a Southern hybridization analysis ...

journal_title：Current genetics

authors： Vai M,Lacanà E,Gatti E,Breviario D,Popolo L,Alberghina L

更新日期：1993-01-01 00:00:00

abstract：:Trichoderma reesei was transformed to hygromycin B resistance using a novel vector, which contains the E. coli hygromycin B phosphotransferase gene (hph) fused between promoter and terminator elements of the homologous Trichoderma pki1 (coding for pyruvate kinase) and cbh2 (coding for cellobiohydrolase II) genes, resp...

journal_title：Current genetics

authors： Mach RL,Schindler M,Kubicek CP

更新日期：1994-06-01 00:00:00

abstract：:The steady-state transcript levels for psbA, psbD, psaA and rbcL are low in dark-grown rice seedlings as compared to those grown in light. Following seed germination, they accumulate in an age-dependent manner, in dark as well as light, reaching a maximal level on the 7th or 8th day, before a slow decline sets in. But...

journal_title：Current genetics

authors： Kapoor S,Maheshwari SC,Tyagi AK

更新日期：1994-04-01 00:00:00

abstract：:Cyclosporin A is a potent and clinically-important immunosuppressive drug (SandimmunR). It is produced by the fungus Tolypocladium niveum. A transformation system for T. niveum ATCC34921 based on hygromycin selection was established. In order to obtain a T. niveum promoter, the cyclophilin gene was isolated using the ...

journal_title：Current genetics

authors： Weber G,Leitner E

更新日期：1994-11-01 00:00:00

abstract：:The regulation of transcription of the class II yeast transposon Ty 1-17 has been examined using a Ty::LEU2 fusion gene. Deletion analysis has defined a 251 bp region within the coding sequence of the TY A gene which is essential for Ty transcription and is also required for diploid control of Ty expression. This down...

journal_title：Current genetics

authors： Warmington JR,Oliver SG

更新日期：1988-05-01 00:00:00

abstract：:Work in recent years has produced indirect evidence to support the view that the phenomenon of suppressiveness in yeast is the result of the ability of the petite mtDNA to out-replicate the wild-type genome. We have developed a method, based on fluorography of gels containing restriction fragments of radioactively lab...

journal_title：Current genetics

authors： Chambers P,Gingold E

更新日期：1986-01-01 00:00:00

abstract：:The insect pathogenic fungus Metarhizium anisopliae is widely used as an insect biocontrol agent. The M. anisopliae conidium plays an important role in pathogenesis and disease transmission. The aim of this study was to identify genes whose expression is up-regulated during conidiogenous cell development. This is a po...

journal_title：Current genetics

authors： Peng G,Xie L,Hu J,Xia Y

更新日期：2009-06-01 00:00:00

abstract：:SecA is one of seven Sec proteins that comprise the prokaryotic protein translocation apparatus. A chloroplast-encoded secA gene has been identified from the unicellular chromophytic alga Pavlova lutherii. The gene predicts a protein that is related to the SecA proteins of Escherichia coli and Bacillus subtilis. The p...

journal_title：Current genetics

authors： Scaramuzzi CD,Hiller RG,Stokes HW

更新日期：1992-11-01 00:00:00

abstract：:NRE, the nitrogen regulatory protein of Penicillium chrysogenum, contains a single Cys2/Cys2-type zinc-finger motif followed immediately by a highly basic region. The zinc-finger domain was expressed to Escherichia coli as a fusion protein with beta-galactosidase. In order to test the putative DNA-binding ability of N...

journal_title：Current genetics

authors： Haas H,Marzluf GA

更新日期：1995-07-01 00:00:00

abstract：:Meiotic fine-structure maps of two efficient UGA suppressors of Schizosaccharomyces pombe which are known (sup8-e) or inferred (sup10-e) to code for two leucine tRNAs carrying the mutant anticodon U(*)CA (Kohli et al. 1979, 1980a, b; Wetzel et al. 1979; Mao et al. 1981) are presented. In both cases, the recombination ...

journal_title：Current genetics

authors： Munz P,Dorsch-Häsler K,Leupold U

更新日期：1983-04-01 00:00:00

abstract：:In order to facilitate the process of epitope-tagging of yeast proteins, we have constructed two Saccharomyces cerevisiae-Escherichia coli shuttle vectors that allow fusion of a sequence encoding an epitope of the human c-myc protein at the 3' end of any gene. An example of the use of this technique is presented. ...

journal_title：Current genetics

authors： Reisdorf P,Maarse AC,Daignan-Fornier B

更新日期：1993-02-01 00:00:00

abstract：:Ribosome is responsible for protein synthesis and is able to monitor the sequence and structure of the nascent peptide. Such ability plays an important role in determining overall gene expression profile of the bacteria through ribosome stalling and rescuing. In this review, we briefly summarize our current understand...

journal_title：Current genetics

authors： Jin Y,Jin S,Wu W

更新日期：2016-05-01 00:00:00

abstract：:Six ultra violet (UV) mutageneses were performed on the spo76 UV-sensitive mutant of Sordaria macrospora. Spo76 shows an early centromere cleavage associated with an arrest at the first meiotic division and therefore does not form ascospores. Moreover, it exhibits altered pairing structure (synaptonemal complex), reve...

journal_title：Current genetics

authors： Huynh AD,Leblon G,Zickler D

更新日期：1986-01-01 00:00:00

abstract：:In order to develop an eukaryotic vector with the Podospora plasmid, further characterization is required of the mitochondrial DNA into which this plasmid is integrated, a physical map (restriction sites) of the Podospora chondriome (size 95 kb) has been completed. As prerequisite for the establishment of a genetic (f...

journal_title：Current genetics

authors： Kück U,Esser K

更新日期：1982-07-01 00:00:00

abstract：:Like other microorganisms, free-living Candida albicans is mainly present in a three-dimensional multicellular structure, which is called a biofilm, rather than in a planktonic form. Candida albicans biofilms can be isolated from both abiotic and biotic surfaces at various locations within the host. As the number of a...

journal_title：Current genetics

authors： Mathé L,Van Dijck P

更新日期：2013-11-01 00:00:00

abstract：:Cellular heterogeneity is a widespread phenomenon, existing across organisms and serving a crucial role in evolution and cell survival. Genetically identical cells may as a result present in a variety of forms with different gene and protein expressions, as well as oxidation level. As a result, a wide range of methodo...

journal_title：Current genetics

authors： Radzinski M,Reichmann D

更新日期：2019-02-01 00:00:00

abstract：:In yeast (S. cerevisiae) the stringent response is known to include rapid, selective, and severe transcriptional curtailment for genes specifying cytoplasmic rRNAs and r-proteins. We have shown that transcription of the mitochondrial 21S rRNA gene is also congruently and selectively curtailed during the yeast stringen...

journal_title：Current genetics

authors： Cantwell R,McEntee CM,Hudson AP

更新日期：1992-03-01 00:00:00

abstract：:Exonuclease II (ExoII) from Schizosaccharomyces pombe is a 5'-->3' single-stranded DNA exonuclease. We have cloned its gene, exo2, whose nucleotide sequence revealed that ExoII is a homologue of the multifunctional Saccharomyces cerevisiae Sep1 protein (also called Kem1, Xrn1, Rar5, Dst2). S. pombe exo2 null mutants w...

journal_title：Current genetics

authors： Szankasi P,Smith GR

更新日期：1996-09-01 00:00:00

abstract：:A class of large site-specific deletions (del-B) occurs with exceptionally-high frequencies of 10(-3) in the mitochondrial COX1 gene of Mn(2+)-treated yeast cells. This work shows that del-B deletions are associated with COX1 intron aI1. All five deletion mutants studied have their upstream end at the authentic 3' spl...

journal_title：Current genetics

authors： Weiller GF

更新日期：1994-11-01 00:00:00

abstract：:A gene encoding mitochondrial S13 is generally present in the mitochondrial genome of higher plants, but is lacking from the Arabidopsis thaliana mitochondrial genome. Previous research has failed to identify a nuclear gene capable of encoding a mitochondrial S13 protein or the protein itself. Doubts have even been ra...

journal_title：Current genetics

authors： Mollier P,Hoffmann B,Debast C,Small I

更新日期：2002-03-01 00:00:00

abstract：:We describe a new nuclear gene, CBT1 (Cytochrome B Termination), specifically involved in the generation of mature mRNA of cytochrome b in yeast mitochondria. Disruption of CBT1 (corresponding to ORF YKL 208W) results in a respiratory deficiency (no growth on acetate and ethanol, a reduced growth on glycerol, and a mo...

journal_title：Current genetics

authors： Rieger KJ,Aljinovic G,Lazowska J,Pohl TM,Slonimski PP,Aljinovic G

更新日期：1997-09-01 00:00:00

abstract：:The REC1 gene of U. maydis has an important but ill-defined role in DNA recombination and repair. We have examined its role in plasmid-chromosome recombination. Plasmid DNA was linearized at various locations with respect to the cloned U. maydis PYR3 gene and introduced into cells by transformation. Chromosomal integr...

journal_title：Current genetics

authors： Banks GR,Kanuga N,Ealing J,Spanos A,Holden DW

更新日期：1992-12-01 00:00:00