Abstract:
:In the photosynthetic chloroplast mutant PRB2A of Chlamydomonas reinhardtii the psbD mRNA is unstable. Three strains were isolated, in which the underlying site-directed mutation within the psbD 5' UTR (untranslated region) is suppressed. In all three suppressors, psbD RNA levels and RNA 5' maturation are restored to a varying extent, suggesting a tight coupling of RNA stabilization and 5' processing. Expression of the psbA gene is not compromised in these strains. Genetic crosses revealed that the suppressor mutations affect three unlinked nuclear loci, which may encode new factors involved in psbD gene expression.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Nickelsen Jdoi
10.1007/s002940050020subject
Has Abstractpub_date
2000-02-01 00:00:00pages
136-42issue
2eissn
0172-8083issn
1432-0983journal_volume
37pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::A 1,380-bp intervening sequence within the mitochondrial small subunit ribosomal RNA (mt SSU rRNA) gene of the fungus Sclerotinia sclerotiorum has been sequenced and identified as a group-I intron. This is the first report of an intron in the mt SSU rRNA gene. The intron shows close similarity in secondary structure t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00313431
更新日期:1995-01-01 00:00:00
abstract::In the fungus Fusarium sporotrichioides, biosynthesis of trichothecene mycotoxins requires at least three genetic loci: a core 12-gene cluster, a smaller two-gene cluster, and a single-gene locus. Here, we describe the Tri15 gene, which represents a fourth locus involved in trichothecene biosynthesis. Tri15 is predict...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-003-0467-3
更新日期:2004-03-01 00:00:00
abstract::The mitochondrial genomes are one of the most information-rich markers in phylogenetics. The relationships within superorder Dictyoptera have been debated in the literature. However, the closely related termites (Isoptera) are retained as unranked taxon within the order Blattaria (cockroaches). In this work, we sequen...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-012-0365-7
更新日期:2012-04-01 00:00:00
abstract::We have found no evidence for an adaptive response for either lethality or mutagenesis following treatment of Saccharomyces cerevisiae with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The rad6 and rad52 mutants of S. cerevisiae are highly defective in MNNG and ethyl methanesulfonate induced mutagenesis of both statio...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00410912
更新日期:1986-01-01 00:00:00
abstract::Spindle pole body (SPB; the microtubule organizing center in yeast) duplication is essential to form a bipolar spindle. The duplicated SPBs must then separate and migrate to opposite sides of the nucleus. We identified a novel functional relationship in SPB separation between the microtubule stabilizing protein Bik1p/...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0098-6
更新日期:2006-12-01 00:00:00
abstract::DNA analysis of Cephalosporium acremonium revealed that this important fungus for biotechnology contains, apart from chromosomal DNA, two types of extrachromosomal DNA which may be useful for the development of a homologous vector system: 1. Plasmid-like (pl) DNA consisting of circular molecules, but of rather heterog...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00391811
更新日期:1982-08-01 00:00:00
abstract::Retrotransposons are suitable targets for developing molecular markers for population genetics studies. Transposable elements have not yet been isolated from ectomycorrhizal fungi of the genus Tuber. In this paper, we report on the isolation and characterization of Tmt1, an LTR-retrotransposon from Tuber melanosporum....
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-007-0155-9
更新日期:2008-01-01 00:00:00
abstract::Broad bean (Vicia faba) mitochondrial DNA (mtDNA) includes three circular plasmids: mt-plasmid 1 (1,704 ntp), mt-plasmid 2 (1,695 ntp) and mt-plasmid 3 (1,476 ntp). Partially replicated circular forms of these mt-plasmids have been observed in electron microscope preparations. Restriction enzymes that cleave either mt...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00569340
更新日期:1988-08-01 00:00:00
abstract::The occupancy of nucleosomes governs access to the eukaryotic genomes and results from a combination of biophysical features and the effect of ATP-dependent remodelling complexes. Most promoter regions show a conserved pattern characterized by a nucleosome-depleted region (NDR) flanked by nucleosomal arrays. The conse...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0642-y
更新日期:2017-05-01 00:00:00
abstract::The Saccharomyces cerevisiae nuclear gene MRP-S9 was identified as part of the European effort in sequencing chromosome II. MRP-S9 encodes for a hydrophilic and basic protein of 278 amino acids with a molecular mass of 32 kDa. The C-terminal part (aa 153-278) of the MRP-S9 protein exhibits significant sequence similar...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00311878
更新日期:1995-06-01 00:00:00
abstract::Two different cytoplasmic male-sterility (CMS) systems, nap and pol, are found in the oilseed rape (canola) species Brassica napus. Physical mapping studies have previously shown that organizational differences between the sterile pol and fertile cam mitochondrial genomes are restricted to a relatively small region im...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050212
更新日期:1997-04-01 00:00:00
abstract::DNA-damage tolerance (DDT) is employed by eukaryotes to deal with replication blocks on the template strand, and is divided into two parallel pathways that are activated by sequential ubiquitination of proliferating cell nuclear antigen (PCNA) at the Lys164 residue. Rad6-Rad18-mediated PCNA monoubiquitination promotes...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-017-0753-0
更新日期:2018-04-01 00:00:00
abstract::Expression of pho1-encoded acid phosphatase of Schizosaccharomyces pombe has been reported to be regulated by phosphate. In this communication we show that it is also regulated by adenine. Starving adenine auxotrophic strains for adenine leads to a drastic increase of the enzymatic activity while adenine represses thi...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00317923
更新日期:1992-10-01 00:00:00
abstract::The physical positions of the DNA sequences encoding the five consecutive enzyme activities required to metabolise 3-deoxy-D-arabino-heptulosonic acid-7-phosphate to 5-enolpyruvyl-shikimate-3phosphate, which are encoded by the A. nidulans Arom polypeptide have been determined. Subfragments of the Arom locus encoding E...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00384611
更新日期:1987-01-01 00:00:00
abstract::Primary transcripts in higher plant cell organelles undergo a series of essential RNA processing steps, including 5'- and 3'-terminal maturation, intron splicing and RNA editing. Most of the protein factors involved in these RNA processing mechanisms are still unknown. Also, little is known about the environmental and...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-002-0278-y
更新日期:2002-04-01 00:00:00
abstract::Saccharomyces cerevisiae adapts to oxidative, osmotic stress and nutrient deprivation through transcriptional changes, decreased proliferation, and entry into other developmental pathways such as pseudohyphal formation and sporulation. Inositol pyrophosphates are necessary for these cellular responses. Inositol pyroph...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-020-01078-8
更新日期:2020-10-01 00:00:00
abstract::The wild diastatic yeast Saccharomyces cerevisiae NCYC 625 has been shown to be homozygous for the glucoamylase-specifying gene STA2. spoII-1-mapping has positioned STA2 on chromosome II. Expression of STA2 is suppressed in some but not all diploids capable of sporulation, and is also inhibited by unlinked nuclear sup...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00314873
更新日期:1990-04-01 00:00:00
abstract::We have determined the size, the restriction map and the gene order of the mitochondrial genome of the yeast Saccharomyces uvarum. Sequence analysis of the mitochondrial COXII gene confirmed the position of this yeast in the Saccharomyces cerevisiae-like group, near Saccharomyces cerevisiae and Saccharomyces douglasii...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050308
更新日期:1998-01-01 00:00:00
abstract::Cochliobolus heterostrophus has alternate genes (MAT-1 and MAT-2) at its mating-type locus. Transformants of a MAT-1 or a MAT-2 strain carrying a transgene of opposite mating type can self and are dual maters; the transgene, however, promotes development of pseudothecia only, not ascospores. To determine if the reside...
journal_title:Current genetics
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
abstract::In order to study pathogenic mechanisms of the plant pathogen Ascochyta rabiei, conditions for efficient transformation using Agrobacterium-mediated transformation were investigated. Hygromycin B resistance (hph) was superior to geneticin resistance (nptII) for selecting transformants, and the hph gene was more effici...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-005-0048-8
更新日期:2006-04-01 00:00:00
abstract::Wild-type Neurospora crassa, strain Singapore, was transformed with a N. crassa metallothionein promoter/protyrosinase fusion gene. Transformants produced tyrosinase during vegetative growth, as determined by Western analyses and activity assays. This is in sharp contrast to wild-type strains, where this enzyme is onl...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00318214
更新日期:1990-11-01 00:00:00
abstract::The S. cerevisiae strain h56 is a temperature-sensitive mit- mutant containing a single nucleotide substitution in the region 5' to the reading frame of the mitochondrial var1 gene. The mutation decreases the efficiency of processing of a precursor RNA such that little var1 mRNA is produced at the restrictive temperat...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00357168
更新日期:1994-03-01 00:00:00
abstract::Spontaneous and double-strand break (DSB)-induced gene conversion in Saccharomyces cerevisiae was assayed using non-tandem chromosomal direct repeat crosses and plasmid x chromosome crosses. Each cross involved identical ura3 alleles marked with phenotypically silent restriction fragment length polymorphic (RFLP) muta...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050396
更新日期:1998-10-01 00:00:00
abstract::In the filamentous ascomycete fungus Podospora anserina, senescence is associated with dramatic changes of the juvenile mitochondrial genome. During senescence specific regions of the non-senescent genome are excised, ligated and amplified as a plasmid. In this paper, we report our studies on nuclear-mitochondrial ext...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00418411
更新日期:1986-01-01 00:00:00
abstract::Chaperonin 60 (Cpn60) is a well-established marker protein for eukaryotic mitochondria and plastids. In order to determine whether the small double-membrane-bounded organelle posterior to the nucleus in the apicomplexan Cryptosporidium parvum is a mitochondrion, the Cpn60 gene of C. parvum sporozoites ( CpCpn60) was a...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-003-0432-1
更新日期:2003-11-01 00:00:00
abstract::Mono-ubiquitinated histone H2B (H2B-Ub) is important for chromatin regulation of transcription, chromatin assembly, and also influences heterochromatin. In this review, we discuss the effects of H2B-Ub from nucleosome to higher-order chromatin structure. We then assess what is currently known of the role of H2B-Ub in ...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-018-0812-1
更新日期:2018-08-01 00:00:00
abstract::Chromosomes of two closely related yeast strains, the amylolytic Saccharomyces diastaticus and the non-amylolytic Saccharomyces cerevisiae, were resolved by pulsed field gel electrophoresis (PFGE) and orthological field alteration gel electrophoresis (OFAGE). Electrophoretic karyotypes of these two strains are identic...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00405847
更新日期:1988-07-01 00:00:00
abstract::The mitochondrial leucyl-tRNA synthetase (mLRS) of Saccharomyces cerevisiae is involved in both mitochondrial protein synthesis and pre-mRNA splicing. We have created mutations in the regions HIGH, GWD and KMSKS, which are involved in ATP-, amino acid- and tRNA-binding respectively, and which have been conserved in th...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351744
更新日期:1992-07-01 00:00:00
abstract::Sequences hybridizing to mitochondrial DNA probes from Saccharomyces cerevisiae have been mapped in six mitochondrial genomes from the Dekkera/Brettanomyces yeasts and in mtDNA from the closely related Eeniella nana. Sequence order for the 34.5 kbp mtDNA of E. nana is identical to that for mtDNAs from B. custersianus ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00418409
更新日期:1986-01-01 00:00:00
abstract::Mutants which carry a leaky UGA nonsense mutation in the fertility gene ste10 are characterized by a deformed cell morphology which resembles that described in the literature for sterile ras1- (ste5) and ral1 to ral4 mutant cells. Although frequent conjugation attempts are observed in combinations of two ste10 mutant ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00312768
更新日期:1991-07-01 00:00:00