Mutations at three different nuclear loci of Chlamydomonas suppress a defect in chloroplast psbD mRNA accumulation.

Abstract:

:In the photosynthetic chloroplast mutant PRB2A of Chlamydomonas reinhardtii the psbD mRNA is unstable. Three strains were isolated, in which the underlying site-directed mutation within the psbD 5' UTR (untranslated region) is suppressed. In all three suppressors, psbD RNA levels and RNA 5' maturation are restored to a varying extent, suggesting a tight coupling of RNA stabilization and 5' processing. Expression of the psbA gene is not compromised in these strains. Genetic crosses revealed that the suppressor mutations affect three unlinked nuclear loci, which may encode new factors involved in psbD gene expression.

journal_name

Curr Genet

journal_title

Current genetics

authors

Nickelsen J

doi

10.1007/s002940050020

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

136-42

issue

2

eissn

0172-8083

issn

1432-0983

journal_volume

37

pub_type

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