Cryptosporidium parvum Cpn60 targets a relict organelle.

Abstract:

:Chaperonin 60 (Cpn60) is a well-established marker protein for eukaryotic mitochondria and plastids. In order to determine whether the small double-membrane-bounded organelle posterior to the nucleus in the apicomplexan Cryptosporidium parvum is a mitochondrion, the Cpn60 gene of C. parvum sporozoites ( CpCpn60) was analyzed and antibodies were generated for localization of the peptide. Sequence and phylogenetic analyses indicated that CpCpn60 is a mitochondrial isotype and that antibodies against it localize to the rough endoplasmic reticulum-enveloped remnant organelle of C. parvum sporozoites. These data show this organelle is of mitochondrial origin.

journal_name

Curr Genet

journal_title

Current genetics

authors

Riordan CE,Ault JG,Langreth SG,Keithly JS

doi

10.1007/s00294-003-0432-1

subject

Has Abstract

pub_date

2003-11-01 00:00:00

pages

138-47

issue

3

eissn

0172-8083

issn

1432-0983

journal_volume

44

pub_type

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