Abstract:
:Chaperonin 60 (Cpn60) is a well-established marker protein for eukaryotic mitochondria and plastids. In order to determine whether the small double-membrane-bounded organelle posterior to the nucleus in the apicomplexan Cryptosporidium parvum is a mitochondrion, the Cpn60 gene of C. parvum sporozoites ( CpCpn60) was analyzed and antibodies were generated for localization of the peptide. Sequence and phylogenetic analyses indicated that CpCpn60 is a mitochondrial isotype and that antibodies against it localize to the rough endoplasmic reticulum-enveloped remnant organelle of C. parvum sporozoites. These data show this organelle is of mitochondrial origin.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Riordan CE,Ault JG,Langreth SG,Keithly JSdoi
10.1007/s00294-003-0432-1subject
Has Abstractpub_date
2003-11-01 00:00:00pages
138-47issue
3eissn
0172-8083issn
1432-0983journal_volume
44pub_type
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