Abstract:
:A gene of Puccinia graminis f. sp. tritici, coding for the translation elongation factor 1 alpha (EF-1 alpha), was isolated from a P. graminis genomic library using the EF-1 alpha gene sequence of Absidia glauca. The coding region of 1389 nucleotides encodes a polypeptide of 463 amino acids and is interrupted by eight introns. An additional intron is located in the 5' untranslated region. A single transcription start point (tsp) was mapped by primer extension. A cDNA fragment corresponding to P. graminis EF-1 alpha mRNA hybridized with a 1.9-kb-long poly(A+)RNA, sufficient to encode the EF-1 alpha protein. Southern hybridization of digested genomic DNA revealed that two copies of the EF-1 alpha gene exist in the genome of P. graminis.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Schillberg S,Gross P,Tiburzy Rdoi
10.1007/BF00352106subject
Has Abstractpub_date
1995-03-01 00:00:00pages
367-72issue
4eissn
0172-8083issn
1432-0983journal_volume
27pub_type
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