Novel genetic tools for probing individual H3 molecules in each nucleosome.


:In eukaryotes, genomic DNA is packaged into the nucleus together with histone proteins, forming chromatin. The fundamental repeating unit of chromatin is the nucleosome, a naturally symmetric structure that wraps DNA and is the substrate for numerous regulatory post-translational modifications. However, the biological significance of nucleosomal symmetry until recently had been unexplored. To investigate this issue, we developed an obligate pair of histone H3 heterodimers, a novel genetic tool that allowed us to modulate modification sites on individual H3 molecules within nucleosomes in vivo. We used these constructs for molecular genetic studies, for example demonstrating that H3K36 methylation on a single H3 molecule per nucleosome in vivo is sufficient to restrain cryptic transcription. We also used asymmetric nucleosomes for mass spectrometric analysis of dependency relationships among histone modifications. Furthermore, we extended this system to the centromeric H3 isoform (Cse4/CENP-A), gaining insights into centromeric nucleosomal symmetry and structure. In this review, we summarize our findings and discuss the utility of this novel approach.


Curr Genet


Current genetics


Ichikawa Y,Kaufman PD




Has Abstract


2019-04-01 00:00:00














  • A putative ABC transporter gene, ZRA1, is required for zearalenone production in Gibberella zeae.

    abstract::Zearalenone (ZEA) is a secondary metabolite produced by various Fusarium species and causes estrogenic disorders in humans and animals. Recent studies have identified the ZEA biosynthesis gene cluster in F. graminearum, but other genes such as transporters responsible for ZEA export have not been identified in the clu...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Lee S,Son H,Lee J,Lee YR,Lee YW

    更新日期:2011-10-01 00:00:00

  • Inheritance and alteration of transforming DNA during an induced parasexual cycle in the imperfect fungus Cladosporium fulvum.

    abstract::Protoplasts of a pAN7-1-transformed isolate of Cladosporium fulvum race 4, harbouring a tandem duplication of the vector, and an untransformed race 5 isolate were fused, without selection for the presence of vector sequences. Fusion products were allowed to haploidize spontaneously. The inheritance of pAN7-1 sequences...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Arnau J,Oliver RP

    更新日期:1993-05-01 00:00:00

  • Isolation and characterization of yeast mutants with thermoconditional sensitivity to the bifunctional alkylating agent nitrogen mustard.

    abstract::Selection of mutants of Saccharomyces cerevisiae sensitive to the DNA cross-linking agent nitrogen mustard (HN2) at two temperatures (23 °C and 36 °C) yielded two isolates with thermoconditionally enhanced (ts) sensitivity to the mutagen. Both were due to single recessive nuclear genes. Mutant allele snm1-2 (ts) showe...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Siede W,Brendel M

    更新日期:1981-11-01 00:00:00

  • New and efficient method using Saccharomyces cerevisiae mutants for identification of siderophores produced by microorganisms.

    abstract::The separation and identification of siderophores produced by microorganisms is a time-consuming and an expensive procedure. We have developed a new and efficient method to identify siderophores using well-established Saccharomyces cerevisiae deletion mutants. The Deltafet3,arn strains fail to sustain growth, even whe...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Park YS,Kim JH,Chang HI,Kim SW,Paik HD,Kang CW,Kim TH,Sung HC,Yun CW

    更新日期:2007-09-01 00:00:00

  • A recessive mutant allele of the HNM1 gene of Saccharomyces cerevisiae is responsible for hyper-resistance to nitrogen mustard.

    abstract::A screening of haploid yeast strains for enhanced resistance to nitrogen mustard (HN2) yielded a recessive mutant allele, hnm1, that conferred hyper-resistance (HYR) to HN2. Diploids, homo- or heterozygous for the HNM1 locus, exhibit normal wild-type like resistance while homozygosity for hnm1 leads to the phenotype H...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Haase E,Brendel M

    更新日期:1990-10-01 00:00:00

  • The intergenic region between the Vicia faba chloroplast tRNA(CAALeu) and tRNA(UAALeu) genes contains a partial copy of the split tRNA(UAALeu) gene.

    abstract::A cluster of three tRNA genes located on fragment Bam6a from Vicia faba chloroplast DNA has been sequenced: it contains the genes for tRNA(CAALeu), tRNA(UAALeu) and tRNA(Phe). The two tRNA(Leu) genes are separated by 443 bp and are transcribed divergently from different DNA strands. The intergenic region contains a se...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Bonnard G,Weil JH,Steinmetz A

    更新日期:1985-01-01 00:00:00

  • Integrative and replicative genetic transformation of Aureobasidium pullulans.

    abstract::A hybrid selectable marker for transformation was constructed by placing the promoter (TEF1p) from the gene encoding the Aureobasidium pullulans translation elongation factor 1-alpha (TEF1) adjacent to the 5' end of the Escherichia coli hygromycin B phosphotransferase gene (HPT). Plasmids containing this hybrid gene (...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Thornewell SJ,Peery RB,Skatrud PL

    更新日期:1995-12-01 00:00:00

  • Inactivation of a single-2A phosphoprotein phosphatase is lethal in Neurospora crassa.

    abstract::A PCR approach, employing the use of degenerate oligonucleotide mixtures, was used to isolate pph-1, a type-2A protein phosphatase (catalytic subunit)-encoding gene, from Neurospora crassa. The isolated single copy gene is 1327 nucleotides in length, contains four putative introns and encodes a 310 amino-acid polypept...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Yatzkan E,Yarden O

    更新日期:1995-10-01 00:00:00

  • Molecular karyotypes for Alternaria plant pathogens known to produce host-specific toxins.

    abstract::There are at least ten plant diseases caused by Alternaria species in which host-specific toxins (HSTs) are responsible for fungal pathogenicity. Of these HST-producers, seven are considered distinct pathotypes of the species Alternaria alternata, and the remaining three are among other species of pathogenic Alternari...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Akamatsu H,Taga M,Kodama M,Johnson R,Otani H,Kohmoto K

    更新日期:1999-07-01 00:00:00

  • Enhanced canavanine uptake is associated with nucleotide permeability in a thymidylate auxotroph of Saccharomyces cerevisiae.

    abstract::The recovery of spontaneous canavanine-resistant mutants is reduced dramatically in a strain of Saccharomyces cerevisiae that carries a suppressed can 1-100 allele and is permeable to and auxotrophic for thymidylate. This effect does not occur in an isogenic strain that neither takes up nor requires the nucleotide. Ho...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Kohalmi SE,Kunz BA

    更新日期:1989-02-01 00:00:00

  • Genetic variability and relatedness in the complex group of black Aspergilli based on random amplification of polymorphic DNA.

    abstract::A PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), was used for assessing genomic variability among a wide range of culture collection strains of black Aspergilli and related species. The performance of this technique is compared with that of the two other genetic techniques most comm...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Megnegneau B,Debets F,Hoekstra RF

    更新日期:1993-01-01 00:00:00

  • Evolutionary conservation of genomic sequences related to the GGP1 gene encoding a yeast GPI-anchored glycoprotein.

    abstract::The GGP1 gene encodes the only GPI-anchored glycoprotein (gp115) that has been purified to-date in the budding yeast Saccharomyces cerevisiae. It is a single-copy gene whose deduced amino-acid sequence shares no significant homology to any other known protein. In this paper we report a Southern hybridization analysis ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Vai M,Lacanà E,Gatti E,Breviario D,Popolo L,Alberghina L

    更新日期:1993-01-01 00:00:00

  • Screening for amyloid proteins in the yeast proteome.

    abstract::The search for novel pathological and functional amyloids represents one of the most important tasks of contemporary biomedicine. Formation of pathological amyloid fibrils in the aging brain causes incurable neurodegenerative disorders such as Alzheimer's, Parkinson's Huntington's diseases. At the same time, a set of ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Ryzhova TA,Sopova JV,Zadorsky SP,Siniukova VA,Sergeeva AV,Galkina SA,Nizhnikov AA,Shenfeld AA,Volkov KV,Galkin AP

    更新日期:2018-04-01 00:00:00

  • An adaptive response to alkylating agents in Aspergillus nidulans.

    abstract::A simple method is described for demonstrating adaptation to alkylation damage in Aspergillus nidulans. One wild type, two MNNG-sensitive, and one MNNG-resistant strain all showed improvement in colony growth when challenged with MNNG following appropriate inducing pretreatments. Other alkylating agents (MMS, EMS) cou...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Hooley P,Shawcross SG,Strike P

    更新日期:1988-11-01 00:00:00

  • Expression of the mitochondrial split gene coding for cytochrome oxidase subunit I in S. cerevisiae: RNA splicing pathway.

    abstract::We have studied the splicing pathway leading to the synthesis of cytochrome oxidase subunit I (COX I) mRNA, by analysing the transcription pattern of several oxi3- splicing deficient mutants located in the first four introns of the gene. The four introns contain long open reading frames (ORFs) in phase with the upstre...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Carignani G,Netter P,Bergantino E,Robineau S

    更新日期:1986-01-01 00:00:00

  • The expression of many chemoreceptor genes depends on the cognate chemoeffector as well as on the growth medium and phase.

    abstract::Chemoreceptor-based signaling is a major bacterial signal transduction mechanism. Escherichia coli, the traditional model, has five chemoreceptors. Recent genome analyses have shown that many bacteria have a much higher number of chemoreceptors. Pseudomonas putida KT2440 is an alternative model that has 27 chemorecept...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: López-Farfán D,Reyes-Darias JA,Krell T

    更新日期:2017-06-01 00:00:00

  • Group I intron lateral transfer between red and brown algal ribosomal RNA.

    abstract::How group I introns originate in nuclear ribosomal (r)RNA genes is an important question in evolutionary biology. Central to this issue is the multitude of group I introns present in evolutionarily distantly related plant, fungal, and protist lineages, together with an understanding of their origin and lateral transfe...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Bhattacarya D,Cannone JJ,Gutell RR

    更新日期:2001-08-01 00:00:00

  • Natural translocation of a large segment of chromosome III to chromosome I in a laboratory strain of Saccharomyces cerevisiae.

    abstract::We have investigated chromosomal segregation during meiosis in a cross between two polymorphic haploid laboratory strains of Saccharomyces cerevisiae, FL100 and GRF18. These two strains have large chromosome-length polymorphisms for chromosomes I and III allowing for easy scoring of parental chromosomes after meiotic ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Camasses A

    更新日期:1996-08-01 00:00:00

  • A mutated swi4 gene causes duplications in the mating-type region of Schizosaccharomyces pombe.

    abstract::Efficient mating-type (MT) switching in homothallic strains of Schizosaccharomyces pombe is significantly reduced if they have a mutation in any of the eleven known swi genes. The swi4 mutation causes heterothallic as well as homothallic segregants, both of which have duplications in the MT region. In contrast to homo...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Fleck O,Heim L,Gutz H

    更新日期:1990-12-01 00:00:00

  • Ectomycorrhiza-mediated repression of the high-affinity ammonium importer gene AmAMT2 in Amanita muscaria.

    abstract::A main function of ectomycorrhizas, a symbiosis between certain soil fungi and fine roots of woody plants, is the exchange of plant-derived carbohydrates for fungus-derived nutrients. As it is required in large amounts, nitrogen is of special interest. A gene (AmAMT2) coding for a putative fungal ammonium importer was...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Willmann A,Weiss M,Nehls U

    更新日期:2007-02-01 00:00:00

  • Transcriptome analysis of Aspergillus fumigatus exposed to voriconazole.

    abstract::For a comprehensive evaluation of genes that have their expression modulated during exposure of the mycelia to voriconazole, we performed a large-scale analysis of gene expression in Aspergillus fumigatus using a microarray hybridization approach. By comparing the expression of genes between the reference time and aft...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: da Silva Ferreira ME,Malavazi I,Savoldi M,Brakhage AA,Goldman MH,Kim HS,Nierman WC,Goldman GH

    更新日期:2006-07-01 00:00:00

  • The fate of mitochondrial DNAs of mt+ and mt- origin in gametes and zygotes of Chlamydomonas.

    abstract::In order to study the mechanism responsible for the uniparental transmission of the mitochondrial genome in crosses between Chlamydomonas reinhardtii and C. smithii, we have analyzed the fate of mitochondrial DNA during gametogenesis, zygospore differentiation and sporulation by hybridization experiments. Both mt+ and...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Beckers MC,Munaut C,Minet A,Matagne RF

    更新日期:1991-08-01 00:00:00

  • Cloning and expression of Hormoconis resinae glucoamylase P cDNA in Saccharomyces cerevisiae.

    abstract::A cDNA coding for glucoamylase P of Hormoconis resinae was cloned using a synthetic oligonucleotide probe coding for a peptide fragment of the purified enzyme and polyclonal anti-glucoamylase antibodies. Nucleotide-sequence analysis revealed an open reading frame of 1848 base pairs coding for a protein of 616 amino-ac...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Vainio AE,Torkkeli HT,Tuusa T,Aho SA,Fagerström BR,Korhola MP

    更新日期:1993-07-01 00:00:00

  • Comparison of the lignin-degrading white rot fungi Phanerochaete chrysosporium and Sporotrichum pulverulentum at the DNA level.

    abstract::DNA-hybridisation studies showed a close relationship between Phanerochaete chrysosporium ME446, most used in lignin degradation studies, and Sporotrichum pulverulentum Novobranova, the other standard lignin degrading strain. Two other strains of P. chrysosporium were both less related. We show that P. chrysosporium M...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Raeder U,Broda P

    更新日期:1984-09-01 00:00:00

  • Brassica nap cytoplasmic male sterility is associated with expression of a mtDNA region containing a chimeric gene similar to the pol CMS-associated orf224 gene.

    abstract::Two different cytoplasmic male-sterility (CMS) systems, nap and pol, are found in the oilseed rape (canola) species Brassica napus. Physical mapping studies have previously shown that organizational differences between the sterile pol and fertile cam mitochondrial genomes are restricted to a relatively small region im...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: L'Homme Y,Stahl RJ,Li XQ,Hameed A,Brown GG

    更新日期:1997-04-01 00:00:00

  • Arenicin-1-induced apoptosis-like response requires RecA activation and hydrogen peroxide against Escherichia coli.

    abstract::Arenicin-1, a 21-mer antimicrobial peptide exerts significant broad-spectrum antimicrobial activity with membrane-active mechanisms. However, owing to multiple mechanisms of cell death, the antibacterial mechanism of arenicin-1 requires detailed analysis. In the present study, arenicin-1-treated bacteria underwent an ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Lee H,Lee DG

    更新日期:2019-02-01 00:00:00

  • Development of a sufficient and effective procedure for transformation of an oleaginous yeast, Rhodosporidium toruloides DMKU3-TK16.

    abstract::Rhodosporidium toruloides DMKU3-TK16 (TK16), a basidiomycetous yeast isolated in Thailand, can produce a large amount of oil corresponding to approximately 70 % of its dry cell weight. However, lack of a sufficient and efficient transformation method makes further genetic manipulation of this organism difficult. We he...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Tsai YY,Ohashi T,Kanazawa T,Polburee P,Misaki R,Limtong S,Fujiyama K

    更新日期:2017-05-01 00:00:00

  • What might retrotransposons teach us about aging?

    abstract::Retrotransposons are activated as organisms age, based on work from several model systems. Since these mobile DNA elements can promote genome instability, this has raised the possibility that they can contribute to loss of cellular function with age. Many questions remain to be addressed about the relationship between...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Maxwell PH

    更新日期:2016-05-01 00:00:00

  • Neurospora crassa nuclear genome contains analogy of Saccharomyces cerevisiae genes for ribosomal RNA processing.

    abstract::Neurospora crassa wild type genome shows DNA sequences which are homologous to the sequences present in the rRNA processing genes of the yeast Saccharomyces cerevisiae. Five such processing genes from yeast, viz., RNA1 through RNA5, cloned in plasmid pBR322 were transformed in Escherichia coli strain LE392. Southern b...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Dutta SK,Verma M,Verma M

    更新日期:1987-01-01 00:00:00

  • Molecular cloning of the meiosis-induced rec10 gene of Schizosaccharomyces pombe.

    abstract::The meiotic recombination gene rec10, which encodes a region-specific activator of recombination in Schizosaccharomyces pombe, has been cloned by genetic complementation and its nucleotide sequence determined. The rec10 gene was identified in a 5.6-kb cloned fragment by partial-deletion and insertion experiments. The ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Lin Y,Smith GR

    更新日期:1995-04-01 00:00:00