Abstract:
:We have characterised a cDNA clone and a nuclear gene encoding the chloroplast 30 s ribosomal protein S13 from Arabidopsis thaliana. The identification is based on the high similarity of the predicted amino-acid sequence with eubacterial S13 protein sequences, and immunodetection of a 14.5-kDa chloroplast ribosomal polypeptide using antibodies raised against the polypeptide produced from part of the cDNA expressed in bacteria. The predicted amino-acid sequence contains an N-terminal extension which has several features characteristic of chloroplast transit peptides. Experiments suggest there is a single copy of this gene in A. thaliana and multiple copies in Brassica species. The origin of the mitochondrial S13 polypeptide in crucifers is also discussed.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Kumar R,Drouaud J,Raynal M,Small Idoi
10.1007/BF00326432subject
Has Abstractpub_date
1995-09-01 00:00:00pages
346-52issue
4eissn
0172-8083issn
1432-0983journal_volume
28pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::Yeast ornithine transaminase is known to be induced by arginine and ornithine, through the action of regulatory elements common to arginase induction. We show here that it is subject to a second induction circuit, that which is responsible for urea amidolyase and urea permease induction by allophanate and defined by t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00376788
更新日期:1981-09-01 00:00:00
abstract::Ten dominant omnipotent suppressors of Saccharomyces cerevisiae, which were previously shown to be different from SUP46, have been examined. Nine are mapped in a region between lys5 and cyh2 on the left arm of chromosome VII. These suppressors, like SUP46, manifest sensitivity to increased temperature and the antibiot...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00340710
更新日期:1989-12-01 00:00:00
abstract::The HOG pathway is an important mitogen-activated protein kinase (MAPK) signal transduction pathway in Saccharomyces cerevisiae that mediates adaptation of cells to hyper-osmotic stress. Activation of this pathway causes rapid but transient, phosphorylation of the MAPK Hog1p. Phosphorylated Hog1p is rapidly transporte...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-005-0010-9
更新日期:2005-09-01 00:00:00
abstract::Six ultra violet (UV) mutageneses were performed on the spo76 UV-sensitive mutant of Sordaria macrospora. Spo76 shows an early centromere cleavage associated with an arrest at the first meiotic division and therefore does not form ascospores. Moreover, it exhibits altered pairing structure (synaptonemal complex), reve...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00447389
更新日期:1986-01-01 00:00:00
abstract::Phyto-pathogenic fungi can cause huge damage to crop production. During millions of years of coexistence, fungi have evolved diverse life-style to obtain nutrients from the host and to colonize upon them. They deploy various proteinaceous as well as non-proteinaceous secreted molecules commonly referred as effectors t...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-020-01118-3
更新日期:2020-11-04 00:00:00
abstract::The meiotic recombination gene rec10, which encodes a region-specific activator of recombination in Schizosaccharomyces pombe, has been cloned by genetic complementation and its nucleotide sequence determined. The rec10 gene was identified in a 5.6-kb cloned fragment by partial-deletion and insertion experiments. The ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00311213
更新日期:1995-04-01 00:00:00
abstract::DNA analysis of Cephalosporium acremonium revealed that this important fungus for biotechnology contains, apart from chromosomal DNA, two types of extrachromosomal DNA which may be useful for the development of a homologous vector system: 1. Plasmid-like (pl) DNA consisting of circular molecules, but of rather heterog...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00391811
更新日期:1982-08-01 00:00:00
abstract::In the photosynthetic chloroplast mutant PRB2A of Chlamydomonas reinhardtii the psbD mRNA is unstable. Three strains were isolated, in which the underlying site-directed mutation within the psbD 5' UTR (untranslated region) is suppressed. In all three suppressors, psbD RNA levels and RNA 5' maturation are restored to ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050020
更新日期:2000-02-01 00:00:00
abstract::The chloroplast genome of black pine (Pinus thunbergii), a gymnosperm, contains 32 different tRNA genes, 30 of which correspond to those previously identified in tobacco and rice chloroplast genomes. Two additional genes encode tRNA(Pro) (GGG) and tRNA(Arg) (CCG); the former is newly identified while the latter is pre...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00313804
更新日期:1994-08-01 00:00:00
abstract::Group-I introns, containing open reading frames (ORFs) that code for homing endonucleases, are widely distributed amongst eukaryotic organellar genomes. However, endonucleases of the GIY-YIG subclass have a restricted distribution in mitochondria and bacteriophages, and have never been observed in plastids. We have fo...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00311888
更新日期:1995-06-01 00:00:00
abstract::Thirteen chromosomal-sized DNA bands of the cultivated mushroom Agaricus bisporus have been resolved using the method of clamped homogeneous electric field (CHEF) electrophoresis. Using chromosome size standards from Schizosaccharomyces pombe, Saccharomyces cerevisiae and Candida albicans, the estimated size of the ch...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351712
更新日期:1993-12-01 00:00:00
abstract::Single-strand breaks (SSB) are discontinuities in one strand of the DNA double helix and are the most common type of damages that arise in cells. SSBs arise mainly from direct attack by intracellular metabolites, however, also essential nuclear processes generate SSBs as intermediates. During the catalytic cycle of DN...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-019-00984-w
更新日期:2019-10-01 00:00:00
abstract::Ime1 plays a pivotal role in the initiation of meiosis in a/alpha diploid cells of Saccharomyces cerevisiae. In the absence of glucose and nitrogen, IME1 expression is greater in a/alpha cells than in either a or alpha cells and therefore only a/alpha, but not a/a or alpha/alpha, cells are committed to sporulation. It...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050332
更新日期:1998-04-01 00:00:00
abstract::The wheat mitochondrial (mt) cox3 has been localized and sequenced. The gene exists as a single copy in the wheat mt master chromosome and is transcribed into a single 1.2 kb RNA, whose extremities have been mapped. Comparison of the wheat and Oenothera cox3 sequences gives ambiguous indications concerning the amino a...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00313247
更新日期:1990-01-01 00:00:00
abstract::We recently discovered the novel non-chromosomal determinant in Saccharomyces cerevisiae [NSI(+)] (nonsense suppression inducer), which causes omnipotent nonsense suppression in strains where the Sup35 N-terminal domain is deleted. [NSI(+)] possesses yeast prion features and does not correspond to previously identifie...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-011-0363-1
更新日期:2012-02-01 00:00:00
abstract::We have determined the nucleotide sequence of a region of the mitochondrial genome of the yeast Saccharomyces douglasii which contains the ATPase subunit 9 gene and part of the intergenic sequences that surround it. The gene is 228 nucleotides long and encodes a polypeptide of 76 aa. A comparison of the coding sequenc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351669
更新日期:1994-06-01 00:00:00
abstract::To supply abundant ribosomes, multiple copies of ribosomal RNA genes (rDNA) are conserved from bacterial to human cells. In eukaryotic genomes, clusters of tandemly repeated rDNA units are present, and their number is stably maintained. Due to high level of transcription of rRNA genes, the repetitive structure is pron...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-019-00956-0
更新日期:2019-08-01 00:00:00
abstract::Expression of pho1-encoded acid phosphatase of Schizosaccharomyces pombe has been reported to be regulated by phosphate. In this communication we show that it is also regulated by adenine. Starving adenine auxotrophic strains for adenine leads to a drastic increase of the enzymatic activity while adenine represses thi...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00317923
更新日期:1992-10-01 00:00:00
abstract::A gene which confers resistance to the systemic fungicide carboxin (Cbx) has been isolated from the maize pathogen, Ustilago maydis, by transferring a plasmid gene library from a Cbx-resistant mutant strain into a sensitive strain and selecting for expression of the resistance gene. Five plasmids, rescued from transfo...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00312739
更新日期:1991-06-01 00:00:00
abstract::The sequence heterogeneity of the ribosomal internal transcribed spacer (ITS) region was investigated for Rhizoctonia cerealis isolates from the anastomosis group AG-DI. Although sequence variability of the ITS has been reported in a few multinucleate R. solani isolates, it has very rarely been reported in binucleate ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-013-0397-7
更新日期:2014-02-01 00:00:00
abstract::Like other microorganisms, free-living Candida albicans is mainly present in a three-dimensional multicellular structure, which is called a biofilm, rather than in a planktonic form. Candida albicans biofilms can be isolated from both abiotic and biotic surfaces at various locations within the host. As the number of a...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-013-0400-3
更新日期:2013-11-01 00:00:00
abstract::Somatic fusion between cells of Chlamydomonas containing complementing cell wall and auxotrophic mutations and bearing chloroplast markers for resistance to antibiotics (streptomycin or spectinomycin) have been performed to analyze the mode of chloroplast gene transmission in the fusion products. Prototrophic colonies...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00419578
更新日期:1981-04-01 00:00:00
abstract::Most eukaryotes require molecular oxygen for growth. In general, oxygen is the terminal electron acceptor of the respiratory chain and represents an important substrate for the biosynthesis of cellular compounds. However, in their natural environment, such as soil, and also during the infection, filamentous fungi are ...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-015-0487-9
更新日期:2015-08-01 00:00:00
abstract::A Neurospora crassa cytoplasmic ribosomal protein gene, crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolate crp-5 genomic sequences. Computer analysis of the DNA sequences sho...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00310894
更新日期:1993-01-01 00:00:00
abstract::Phosphatidylcholines (PCs) are a class of major cell membrane phospholipids that participate in many physiological processes. Three genes, choA, choB and choC, have been proposed to function in the endogenous biosynthesis of PC in Aspergillus nidulans. In this study, we characterize the choC gene encoding a putative h...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-010-0300-8
更新日期:2010-06-01 00:00:00
abstract::The var1 locus on yeast mitochondria) DNA is defined by a set of alleles that affect the apparent size (Mr 40,000-44,000) of a mitochondria) translation product (var1), a protein of the mitochondria) 38S ribosomal subunit. Using petite deletion and restriction endonuclease site mapping, we have defined the physical lo...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00445691
更新日期:1980-07-01 00:00:00
abstract::Primary transcripts in higher plant cell organelles undergo a series of essential RNA processing steps, including 5'- and 3'-terminal maturation, intron splicing and RNA editing. Most of the protein factors involved in these RNA processing mechanisms are still unknown. Also, little is known about the environmental and...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-002-0278-y
更新日期:2002-04-01 00:00:00
abstract::Mitochondrial RNA from grande yeast was analyzed by electrophoresis on agarose-urea, acrylamide-urea, and methyl mercuric hydroxide-agarose gels. These gel systems display more than 40 RNA bands that copurify with mitochondria; these bands are not present in cytoplasmic RNA preparations. Analysis of molecular weight o...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00446962
更新日期:1980-02-01 00:00:00
abstract::Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNN...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351689
更新日期:1992-04-01 00:00:00
abstract::The mitochondrial leucyl-tRNA synthetase (mLRS) of Saccharomyces cerevisiae is involved in both mitochondrial protein synthesis and pre-mRNA splicing. We have created mutations in the regions HIGH, GWD and KMSKS, which are involved in ATP-, amino acid- and tRNA-binding respectively, and which have been conserved in th...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351744
更新日期:1992-07-01 00:00:00