Characterization of the nuclear gene encoding chloroplast ribosomal protein S13 from Arabidopsis thaliana.

Abstract:

:We have characterised a cDNA clone and a nuclear gene encoding the chloroplast 30 s ribosomal protein S13 from Arabidopsis thaliana. The identification is based on the high similarity of the predicted amino-acid sequence with eubacterial S13 protein sequences, and immunodetection of a 14.5-kDa chloroplast ribosomal polypeptide using antibodies raised against the polypeptide produced from part of the cDNA expressed in bacteria. The predicted amino-acid sequence contains an N-terminal extension which has several features characteristic of chloroplast transit peptides. Experiments suggest there is a single copy of this gene in A. thaliana and multiple copies in Brassica species. The origin of the mitochondrial S13 polypeptide in crucifers is also discussed.

journal_name

Curr Genet

journal_title

Current genetics

authors

Kumar R,Drouaud J,Raynal M,Small I

doi

10.1007/BF00326432

subject

Has Abstract

pub_date

1995-09-01 00:00:00

pages

346-52

issue

4

eissn

0172-8083

issn

1432-0983

journal_volume

28

pub_type

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