The fate of mitochondrial DNAs of mt+ and mt- origin in gametes and zygotes of Chlamydomonas.

abstract：:By using mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) as genetic markers, the modes of mitochondrial inheritance in sexual crosses and protoplast cell fusions of the higher basidiomycete Lentinula edodes were examined. All newly established dikaryons from reciprocal crosses between compa...

journal_title：Current genetics

authors： Fukuda M,Harada Y,Imahori S,Fukumasa-Nakai Y,Hayashi Y

更新日期：1995-05-01 00:00:00

abstract：:Primary transcripts in higher plant cell organelles undergo a series of essential RNA processing steps, including 5'- and 3'-terminal maturation, intron splicing and RNA editing. Most of the protein factors involved in these RNA processing mechanisms are still unknown. Also, little is known about the environmental and...

journal_title：Current genetics

authors： Karcher D,Bock R

更新日期：2002-04-01 00:00:00

abstract：:In the multicellular red alga Antithamnion spec. both rubisco genes (rbcL and rbcS) are encoded on the plastid DNA (ptDNA). Both genes are separated by a short A/T-rich spacer of 100 bp and are cotranscribed into an mRNA of approximately 2.7 kb. These findings are in extensive agreement with those obtained from two un...

journal_title：Current genetics

authors： Kostrzewa M,Valentin K,Maid U,Radetzky R,Zetsche K

更新日期：1990-12-01 00:00:00

abstract：:Similarity searches with Didymium iridis mitochondrial genomic DNA identified six possible ribosomal protein-coding regions, however, each region contained stop codons that would need to be removed by RNA editing to produce functional transcripts. RT-PCR was used to amplify these regions from total RNA for cloning and...

journal_title：Current genetics

authors： Hendrickson PG,Silliker ME

更新日期：2010-06-01 00:00:00

abstract：:The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was inducted by (1) efficient expr...

journal_title：Current genetics

authors： Tudzynski P,Esser K

更新日期：1983-04-01 00:00:00

abstract：:The yeast OMP decarboxylase gene has been cloned in the phage fd in both orientations to obtain pure DNA of each strand. With these probes the transcription of each strand of the yeast gene was measured in wild type yeast, either repressed or induced, in a yeast strain transformed by an hybrid plasmid containing the O...

journal_title：Current genetics

authors： Hubert JC,Bach ML,Lacroute F

更新日期：1980-10-01 00:00:00

abstract：:Chaperonin 60 (Cpn60) is a well-established marker protein for eukaryotic mitochondria and plastids. In order to determine whether the small double-membrane-bounded organelle posterior to the nucleus in the apicomplexan Cryptosporidium parvum is a mitochondrion, the Cpn60 gene of C. parvum sporozoites ( CpCpn60) was a...

journal_title：Current genetics

authors： Riordan CE,Ault JG,Langreth SG,Keithly JS

更新日期：2003-11-01 00:00:00

abstract：:Selection of mutants of Saccharomyces cerevisiae sensitive to the DNA cross-linking agent nitrogen mustard (HN2) at two temperatures (23 °C and 36 °C) yielded two isolates with thermoconditionally enhanced (ts) sensitivity to the mutagen. Both were due to single recessive nuclear genes. Mutant allele snm1-2 (ts) showe...

journal_title：Current genetics

authors： Siede W,Brendel M

更新日期：1981-11-01 00:00:00

abstract：:cAMP-dependent protein kinases (PKA) are ubiquitous signalling molecules that mediate many extracellular signals in eukaryotes from yeast to men. Directing PKA to its substrates is an important level of control to ensure specificity of cAMP-mediated signal transduction. Unlike in yeast and fungi, in mammalian cells a ...

journal_title：Current genetics

authors： Griffioen G,Thevelein JM

更新日期：2002-07-01 00:00:00

abstract：:In order to study resistance to DNA damaging agents, yeast DNA segments conferring hyperresistance in this organism to such genotoxic agents were selected for among yeast cells transformed by a yeast genome library based on the multi-copy vector plasmid YEp13. Genetic variants hyperresistant to 4-nitroquinoline-N-oxid...

journal_title：Current genetics

authors： Ruhland A,Brendel M,Haynes RH

更新日期：1986-01-01 00:00:00

abstract：:We have analysed two new genes, YBR1007 and YBR1015, discovered during the systematic sequencing of chromosome II of S. cerevisiae. YBR1007 shows strong similarities to amino-acid permeases, in particular the high-affinity proline permeases of S. cerevisiae and A. nidulans. The number and position of the predicted mem...

journal_title：Current genetics

authors： Nasr F,Bécam AM,Grzybowska E,Zagulski M,Slonimski PP,Herbert CJ

更新日期：1994-07-01 00:00:00

abstract：:Five haploid monokaryons of the basidiomycete Coprinus cinereus were already known to be unable to form sclerotia (asexual resting structures) on the vegetative monokaryotic mycelium. Genetic analyses had shown that four distinct genes (symbolised scl were represented, all being "recessive" to their sclerotium-produci...

journal_title：Current genetics

authors： Moore D

更新日期：1981-05-01 00:00:00

abstract：:The sequence of a representative mitochondrial gene COXI, encoding cytochrome c oxidase subunit I, was determined in five species that cover all the orders of the Prymnesiophyta with the exception of the Pavlovales. Through this analysis, we noticed that the 'stop' codon UGA appears frequently and, specifically, at co...

journal_title：Current genetics

authors： Hayashi-Ishimaru Y,Ehara M,Inagaki Y,Ohama T

更新日期：1997-10-01 00:00:00

abstract：:O-Mannosylation is suggested to be essential for protein secretion in Trichoderma reesei. In protein O-glycosylation, the first mannosyl residue is transferred to a serine or threonine hydroxyl group of the protein from dolichyl phosphate mannose by protein O-mannosyltransferase. In Saccharomyces cerevisiae, seven PMT...

journal_title：Current genetics

authors： Zakrzewska A,Migdalski A,Saloheimo M,Penttila ME,Palamarczyk G,Kruszewska JS

更新日期：2003-04-01 00:00:00

abstract：:In order to study pathogenic mechanisms of the plant pathogen Ascochyta rabiei, conditions for efficient transformation using Agrobacterium-mediated transformation were investigated. Hygromycin B resistance (hph) was superior to geneticin resistance (nptII) for selecting transformants, and the hph gene was more effici...

journal_title：Current genetics

authors： White D,Chen W

更新日期：2006-04-01 00:00:00

abstract：:The steady-state transcript levels for psbA, psbD, psaA and rbcL are low in dark-grown rice seedlings as compared to those grown in light. Following seed germination, they accumulate in an age-dependent manner, in dark as well as light, reaching a maximal level on the 7th or 8th day, before a slow decline sets in. But...

journal_title：Current genetics

authors： Kapoor S,Maheshwari SC,Tyagi AK

更新日期：1994-04-01 00:00:00

abstract：:Prototrophic hybrids have been obtained by the fusion of auxotrophic haploid strains of the two yeasts Yarrowia (Saccharomycopsis) lipolytica and Kluyveromyces lactis. The hybrid fusants had a colonial morphology intermediate between that of the two parent strains, were uninucleate, and contained an approximately dipl...

journal_title：Current genetics

authors： Groves DP,Oliver SG

更新日期：1984-01-01 00:00:00

abstract：:A PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), was used for assessing genomic variability among a wide range of culture collection strains of black Aspergilli and related species. The performance of this technique is compared with that of the two other genetic techniques most comm...

journal_title：Current genetics

authors： Megnegneau B,Debets F,Hoekstra RF

更新日期：1993-01-01 00:00:00

abstract：:The mother-bud neck is defined as the boundary between the mother cell and bud in budding microorganisms, wherein sequential morphological events occur throughout the cell cycle. This study was designed to quantitatively investigate the morphology of the mother-bud neck in budding yeast Saccharomyces cerevisiae. Obser...

journal_title：Current genetics

authors： Kubo K,Okada H,Shimamoto T,Kimori Y,Mizunuma M,Bi E,Ohnuki S,Ohya Y

更新日期：2019-02-01 00:00:00

abstract：:Uridine diphosphoglucose is not the sole donor for trehalose synthesis in yeast cells: an ADPG-dependent trehalose synthase, has been identified in mutant strains with undetectable UDPG-dependent trehalose-6-P synthase activity. Genetic and chromatographic studies indicate that the two activities correspond to differe...

journal_title：Current genetics

authors： Paschoalin VM,Silva JT,Panek AD

更新日期：1989-08-01 00:00:00

abstract：:Genetic analysis of carotenogenesis in Phycomyces is hampered by the inability of most mutants to complete the sexual cycle. Heterokaryons between complementing mutants or between a mutant and a helper strain are, however, fertile. Using this method crosses have been carried out between mutants representing the differ...

journal_title：Current genetics

authors： Roncero MI,Cerdá-Olmedo E

更新日期：1982-05-01 00:00:00

abstract：:The ribosomal DNA repeat unit of Sphaeronaemella fimicola was found to be a 13.7-kb tandem repeat with a relatively long nontranscribed spacer (NTS) and an unusually compact ribosomal RNA gene cluster. The DNA sequence of an 850-bp PCR amplification product containing the 3' end of the small subunit rRNA (SSrRNA) gene...

journal_title：Current genetics

authors： Hausner G,Klassen GR,Reid J

更新日期：1993-01-01 00:00:00

abstract：:To understand the organization of the mating type locus of Stagonosporopsis tanaceti and Stagonosporopsis chrysanthemi, and its potential role in the epidemiology of ray blight of pyrethrum and chrysanthemum, respectively, the mating type (MAT) locus of these species was cloned and characterized using PCR-based techni...

journal_title：Current genetics

authors： Chilvers MI,Jones S,Meleca J,Peever TL,Pethybridge SJ,Hay FS

更新日期：2014-11-01 00:00:00

abstract：:The cloning and characterization of the Thcut1 gene, which encodes a cutinase protein of the biocontrol fungus Trichoderma harzianum T34, is reported. Cutinases, which are secreted enzymes that hydrolyse cutin, belong to a class of serine esterases able to hydrolyze fatty acid esters and emulsified triglycerides. The ...

journal_title：Current genetics

authors： Rubio MB,Cardoza RE,Hermosa R,Gutiérrez S,Monte E

更新日期：2008-12-01 00:00:00

abstract：:A 4062-nucleotide (nt) fragment of the mitochondrial genome of the crustacean Daphnia pulex was sequenced and found to contain the complete genes for eight tRNAs and five proteins (ATP6, ATP8, COII, COIII, ND3) and the partial sequence of COI. In combination with data described previously, approximately 50% of the D. ...

journal_title：Current genetics

authors： Crease TJ,Little TJ

更新日期：1997-01-01 00:00:00

abstract：:The chloroplast genome of black pine (Pinus thunbergii), a gymnosperm, contains 32 different tRNA genes, 30 of which correspond to those previously identified in tobacco and rice chloroplast genomes. Two additional genes encode tRNA(Pro) (GGG) and tRNA(Arg) (CCG); the former is newly identified while the latter is pre...

journal_title：Current genetics

authors： Tsudzuki J,Ito S,Tsudzuki T,Wakasugi T,Sugiura M

更新日期：1994-08-01 00:00:00

abstract：:A 1,380-bp intervening sequence within the mitochondrial small subunit ribosomal RNA (mt SSU rRNA) gene of the fungus Sclerotinia sclerotiorum has been sequenced and identified as a group-I intron. This is the first report of an intron in the mt SSU rRNA gene. The intron shows close similarity in secondary structure t...

journal_title：Current genetics

authors： Carbone I,Anderson JB,Kohn LM

更新日期：1995-01-01 00:00:00

abstract：:The core enzyme of mitochondrial RNA polymerase in yeast is homologous to those of bacteriophages T3, T7 and SP6. In previous studies the identification of the first conditional yeast mutant for this enzyme helped to identify the corresponding specificity factor and to elucidate their interaction inside mitochondria. ...

journal_title：Current genetics

authors： Lisowsky T,Stein T,Michaelis G,Guan MX,Chen XJ,Clark-Walker GD

更新日期：1996-11-01 00:00:00

abstract：:An Agrocybe aegerita cDNA library, constructed from fruit body primordia poly(A)+ RNAs, was screened by differential colony hybridization. Clones which preferentially hybridized to poly(A)+ RNA sequences from fruit body primordia, versus poly(A)+ RNAs from mycelium, were isolated. Eight of these clones (EMAa-1 to EMAa...

journal_title：Current genetics

authors： Salvado JC,Labarère J

更新日期：1991-08-01 00:00:00

abstract：:Cells of all living organisms contain complex signal transduction networks to ensure that a wide range of physiological properties are properly adapted to the environmental conditions. The fundamental concepts and individual building blocks of these signalling networks are generally well-conserved from yeast to man; y...

journal_title：Current genetics

authors： Smets B,Ghillebert R,De Snijder P,Binda M,Swinnen E,De Virgilio C,Winderickx J

更新日期：2010-02-01 00:00:00