Fission yeast Cdc23 interactions with DNA replication initiation proteins.

abstract：:A 1,380-bp intervening sequence within the mitochondrial small subunit ribosomal RNA (mt SSU rRNA) gene of the fungus Sclerotinia sclerotiorum has been sequenced and identified as a group-I intron. This is the first report of an intron in the mt SSU rRNA gene. The intron shows close similarity in secondary structure t...

journal_title：Current genetics

authors： Carbone I,Anderson JB,Kohn LM

更新日期：1995-01-01 00:00:00

abstract：:A previously unknown nuclear gene DSS-1 from Saccharomyces cerevisiae was cloned and sequenced. The gene was isolated as a multicopy suppressor of a disruption of the SUV-3 gene coding for a DEAD/H box protein involved in processing and turnover of mitochondrial transcripts. The DSS-1 gene codes for a 970 amino-acid p...

journal_title：Current genetics

authors： Dmochowska A,Golik P,Stepien PP

更新日期：1995-07-01 00:00:00

abstract：:O-Mannosylation is suggested to be essential for protein secretion in Trichoderma reesei. In protein O-glycosylation, the first mannosyl residue is transferred to a serine or threonine hydroxyl group of the protein from dolichyl phosphate mannose by protein O-mannosyltransferase. In Saccharomyces cerevisiae, seven PMT...

journal_title：Current genetics

authors： Zakrzewska A,Migdalski A,Saloheimo M,Penttila ME,Palamarczyk G,Kruszewska JS

更新日期：2003-04-01 00:00:00

abstract：:We have sequenced a region (7,376-bp) of the mitochondrial (mt) DNA (54 kb) of the cellular slime mold, Dictyostelium discoideum. From the DNA and amino-acid sequence comparisons with known sequences, genes for ATPase subunit 9 (ATP9), cytochrome b (CYTB), NADH dehydrogenase subunits 1, 3 and 6 (ND1, ND3 and ND6), sma...

journal_title：Current genetics

authors： Angata K,Kuroe K,Yanagisawa K,Tanaka Y

更新日期：1995-02-01 00:00:00

abstract：:ERG24 is the structural gene for the C14-sterol reductase in yeast. A lack of activity in that enzyme, mediated either by the morpholine fungicides or the insertional inactivation of ERG24, causes the accumulation of the aberrant sterol ignosterol. Cells producing this sterol are unable to grow aerobically in the rout...

journal_title：Current genetics

authors： Crowley JH,Tove S,Parks LW

更新日期：1998-08-01 00:00:00

abstract：:DNA replication checkpoint is a cell signaling pathway that is activated in response to perturbed replication. Although it is crucial for maintaining genomic integrity and cell survival, the exact mechanism of the checkpoint signaling remains to be understood. Emerging evidence has shown that RecQ helicases, a large f...

journal_title：Current genetics

authors： Ahamad N,Khan S,Mahdi ATA,Xu YJ

更新日期：2021-01-11 00:00:00

abstract：:Extracellular proteins of filamentous fungi are important for biomedical and biotechnological applications. Aspergillus terreus not only comprises an important class of organisms that have significant commercial relevance to the biotechnology industry, but also is an emerging fungal pathogen. However, no information i...

journal_title：Current genetics

authors： Han MJ,Kim NJ,Lee SY,Chang HN

更新日期：2010-08-01 00:00:00

abstract：:Studies from this laboratory have suggested that mitochondrial (mt) transcription in yeast (Saccharomyces cerevisiae) is governed by changing cellular cAMP levels, and that the mechanism of such transcriptional regulation requires cAMP-dependent protein kinase (PKA) activity; these observations, in turn, suggest a tra...

journal_title：Current genetics

authors： Iqbal J,Gérard HC,Rahman MU,Hudson AP

更新日期：1996-12-01 00:00:00

abstract：:Mixed aeciospore isolates of Cronartium flaccidum and Peridermium pini were obtained from single-tree infections in Britain, Italy and Greece. The 5.8s ribosomal RNA gene and flanking intergenic transcribed spacer regions ITS1 and ITS2 were found to be highly similar between C. flaccidum and P. pini. Within samples he...

journal_title：Current genetics

authors： Moricca S,Kasuga T,Mitchelson K,Ragazzi A,Diamandis S

更新日期：1996-03-01 00:00:00

abstract：:Chitin is a component of the yeast cell wall which is localized to the septum between mother and daughter cells. Previous work in Saccharomyces cerevisiae has shown that this organism possesses three chitin synthases, 1, 2, and 3. Disruption experiments have shown that loss of chitin synthase 2 has a more profound eff...

journal_title：Current genetics

authors： Baymiller J,McCullough JE

更新日期：1993-02-01 00:00:00

abstract：:A 4062-nucleotide (nt) fragment of the mitochondrial genome of the crustacean Daphnia pulex was sequenced and found to contain the complete genes for eight tRNAs and five proteins (ATP6, ATP8, COII, COIII, ND3) and the partial sequence of COI. In combination with data described previously, approximately 50% of the D. ...

journal_title：Current genetics

authors： Crease TJ,Little TJ

更新日期：1997-01-01 00:00:00

abstract：:The Hansenula polymorpha per6-210 mutant is impaired in respect of growth on methanol (Mut-) and is characterized by aberrant peroxisome formation. The functionally complementing DNA fragment contains two open reading frames. The first encodes dihydroxyacetone kinase (DAK), a cytosolic enzyme essential for formaldehyd...

journal_title：Current genetics

authors： van der Klei IJ,van der Heide M,Baerends RJ,Rechinger KB,Nicolay K,Kiel JA,Veenhuis M

更新日期：1998-07-01 00:00:00

abstract：:We have constructed a gene coding for the 12-kDa intermediate form of the 2s methionine-rich protein from Bertholletia excelsa seeds. This protein, expressed intracellularly in yeast, is characterised by a 20-min half-life. By adding 11 amino acids corresponding to the peroxisome-targeting sequence (PTSc) of luciferas...

journal_title：Current genetics

authors： Nicaud JM,Raynal A,Beyou A,Merkamm M,Ito H,Labat N

更新日期：1994-11-01 00:00:00

abstract：:In previous work, four genes involved in mating-type determination were cloned from reference strains of Pyrenopeziza brassicae; three genes, PAD1, PMT1, and PHB1 (re-named henceforth as MAT-1-1, MAT-1-4, and MAT1-3, respectively), are encoded by the MAT-1 idiomorph, and one gene, PHB2 (re-named MAT-2), by the corresp...

journal_title：Current genetics

authors： Singh G,Dyer PS,Ashby AM

更新日期：1999-11-01 00:00:00

abstract：:In filamentous ascomycetes, glucose repression is mediated by CRE1, a zinc-finger protein related to Miglp from yeast. Five putative AMPK phosphorylation motifs identified in the glucose repressor from the phytopathogenic fungus Sclerotinia sclerotiorum were mutated in a GFP::CRE1 translational fusion. Complementation...

journal_title：Current genetics

authors： Vautard-Mey G,Fèvre M

更新日期：2000-05-01 00:00:00

abstract：:The cif1 mutation of Saccharomyces cerevisiae causes inability to grow on glucose and related fermentable carbon sources. We have isolated two different suppressor mutations that allow growth on glucose of yeasts carrying the cif1 mutation. One of them, sci1-1, is recessive and caused inability to grow on non-fermenta...

journal_title：Current genetics

authors： Blázquez MA,Gancedo C

更新日期：1994-02-01 00:00:00

abstract：:Enzymatic and genetic characterization of cpd-1 and cpd-2, which exhibit rhythmic conidiation in liquid media and on solid media, were described with band (bd) strain as a reference. Cpd-1 and cpd-2 showed reduced growth in orthophosphate-free cyclic 3',5'-AMP media, while bd showed wild-type level of growth in the me...

journal_title：Current genetics

authors： Hasunuma K,Shinohara Y

更新日期：1985-01-01 00:00:00

abstract：:A phylogenetic investigation of the autosporic chlorophycean alga species Selenastrum capricornutum using the small subunit (SSU) rRNA gene revealed the unusual presence of six group IC1 introns. Previous studies showed that numerous green algal taxa contain group IC1 introns. However, whereas some algal species harbo...

journal_title：Current genetics

authors： Booton GC,Floyd GL,Fuerst PA

更新日期：2004-10-01 00:00:00

abstract：:Blasticidin S-resistant mutants of S. cerevisiae were isolated and characterized. Resistant mutations were found to fall into two complementation groups. A single recessive nuclear gene was responsible for each group, donated as bls1 and bls2, respectively. A gene bls1 was linked to an ilv3 gene located on the right a...

journal_title：Current genetics

authors： Ishiguro J,Miyazaki M

更新日期：1985-01-01 00:00:00

abstract：:Over the last decade, mutation studies have grown in popularity due to the affordability and accessibility of whole genome sequencing. As the number of species in which spontaneous mutation has been directly estimated approaches 20 across two domains of life, questions arise over the repeatability of results in such e...

journal_title：Current genetics

authors： Behringer MG,Hall DW

更新日期：2016-08-01 00:00:00

abstract：:Fission yeast is a simple eukaryotic model organism in which many aspects of cell cycle control can be explored. We examined by homologous recombination whether the human CDC25A phosphatase could substitute for the function of the fission yeast Cdc25. We first show: (a). that CDC25A efficiently replaces the endogenous...

journal_title：Current genetics

authors： Mondesert O,Lemaire M,Brezak MC,Galera-Contour MO,Prevost G,Ducommun B,Bugler B

更新日期：2004-05-01 00:00:00

abstract：:The generation of a comprehensive EST library and the sequencing of its genome set the stage for reverse genetics approaches in Chlamydomonas reinhardtii. However, these also require tools for the specific downregulation of target gene expression. Consequently, a large number of diverse constructs were developed aimed...

journal_title：Current genetics

authors： Schroda M

更新日期：2006-02-01 00:00:00

abstract：:Cyh3 and cyh4, multiple drug resistant strains of Schizosaccharomyces pombe, show a much reduced uptake of trichodermin, chloramphenicol, cycloheximide, L-lysine, glycine, L-threonine, L-glutamine, L-arginine and L-glutamic acid when compared to wild type. The plasma membrane and mitochondrial ATPase activities of the...

journal_title：Current genetics

authors： Johnston PA,Coddington A

更新日期：1983-07-01 00:00:00

abstract：:The var1 locus on yeast mitochondria) DNA is defined by a set of alleles that affect the apparent size (Mr 40,000-44,000) of a mitochondria) translation product (var1), a protein of the mitochondria) 38S ribosomal subunit. Using petite deletion and restriction endonuclease site mapping, we have defined the physical lo...

journal_title：Current genetics

authors： Vincent RD,Perlman PS,Strausberg RL,Butow RA

更新日期：1980-07-01 00:00:00

abstract：:Emetine resistant mutants have been isolated in the fungus Podospora anserina. Genetic analysis of 75 mutants has shown that resistance is controlled by at least two genes, EmR1 and EmR2. In vitro studies showed that resistance to emetine in EmR1 and EMR2 mutants was due to alterations in the protein synthesis machine...

journal_title：Current genetics

authors： Crouzet M,Bégueret J

更新日期：1982-10-01 00:00:00

abstract：:We have cloned and sequenced human and bovine cDNAs for the beta subunit of the ATP synthase (ATP-syn beta), a nuclear DNA (nDNA) encoded oxidative phosphorylation (OXPHOS) gene. The two cDNAs were found to share 99% amino acid homology and 94% nucleotide homology. The evolutionary rate of ATPsyn beta was then compare...

journal_title：Current genetics

authors： Wallace DC,Ye JH,Neckelmann SN,Singh G,Webster KA,Greenberg BD

更新日期：1987-01-01 00:00:00

abstract：:1710 asci exhibiting mendelian or aberrant segregation at the b8 spore colour locus in Ascobolus were analysed from crosses between b8 mutants and the wild type in the presence of linked flanking markers. The frequencies of associated crossing-over were found to vary with the various classes of aberrant segregation an...

journal_title：Current genetics

authors： Nicolas A

更新日期：1982-11-01 00:00:00

abstract：:URA4, the gene coding for orotidine monophosphate decarboxylase (OMPdecase), has been cloned from the fission yeast by homologous complementation and restricted in an Escherichia coli-Schizosaccharomyces pombe (E. coli-S. pombe) replicative plasmid to a 1.76 kb HindIII fragment. This plasmid is maintained at a high co...

journal_title：Current genetics

authors： Bach ML

更新日期：1987-01-01 00:00:00

abstract：:By functional complementation of the auxotrophic requirements for choline of a cdg1, cho2 double-mutant, by transformation with a genomic DNA library in a high copy number plasmid, two different types of complementing DNA inserts were identified. One type of insert was earlier shown to represent the CHO2 structural ge...

journal_title：Current genetics

authors： Preitschopf W,Lückl H,Summers E,Henry SA,Paltauf F,Kohlwein SD

更新日期：1993-02-01 00:00:00

abstract：:Loss of heterozygosity (LOH) in a vegetatively growing diploid cell signals irregularity of mitosis. Therefore, assays of LOH serve to discover pathways critical for proper replication and segregation of chromosomes. We screened for enhanced LOH in a whole-genome collection of diploid yeast strains in which a single g...

journal_title：Current genetics

authors： Tutaj H,Pogoda E,Tomala K,Korona R

更新日期：2019-04-01 00:00:00