Abstract:
:As part of developing an ex planta model system for the study of maize plastid and mitochondrial gene expression, a series of established Black Mexican Sweet (BMS) suspension cell lines was characterized. Although the initial assumption was that their organelle biochemistry would be similar enough to normal in planta cells to facilitate future work, each of the three lines was found to have plastid DNA (ptDNA) differing from control maize plants, in one case lacking as much as 70% of the genome. The other two BMS lines possessed either near-wild-type ptDNA or displayed an intermediate state of gene loss, suggesting that these clonal lines are rapidly evolving. Gene expression profiles of BMS cells varied dramatically from those in maize leaf chloroplasts, but resembled those of albino plants lacking plastid ribosomes. In spite of lacking most plastid gene expression and apparently mature rRNAs, BMS cells appear to import proteins from the cytoplasm in a normal manner. The regions retained in BMS ptDNAs point to a set of tRNA genes universally preserved among even highly reduced plastid genomes, whereas the other preserved regions may illuminate which plastid genes are truly indispensable for plant cell survival.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Cahoon AB,Cunningham KA,Bollenbach TJ,Stern DBdoi
10.1007/s00294-003-0408-1subject
Has Abstractpub_date
2003-11-01 00:00:00pages
104-13issue
2eissn
0172-8083issn
1432-0983journal_volume
44pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::Chloroplast DNAs from six different laboratory collections of Euglena gracilis "strain Z" and var. bacillaris were analyzed with restriction endonucleases EcoRI and Bam HI. The most variable portion of the organelle genome is the region containing the ribosomal cistrons. Intraspecific differences occur in both ribosom...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00429820
更新日期:1981-07-01 00:00:00
abstract::A previously unknown nuclear gene DSS-1 from Saccharomyces cerevisiae was cloned and sequenced. The gene was isolated as a multicopy suppressor of a disruption of the SUV-3 gene coding for a DEAD/H box protein involved in processing and turnover of mitochondrial transcripts. The DSS-1 gene codes for a 970 amino-acid p...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00315775
更新日期:1995-07-01 00:00:00
abstract::The cytoplasmic virus-like element pWR1A from Debaryomyces robertsiae encodes a toxin (DrT) with similarities to the Pichia acaciae killer toxin PaT, which acts by importing a toxin subunit (PaOrf2) with tRNA anticodon nuclease activity into target cells. As for PaT, loss of the tRNA methyltransferase Trm9 or overexpr...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-014-0426-1
更新日期:2014-08-01 00:00:00
abstract::An electrophoretic karyotype of the filamentous fungus Podospora anserina has been obtained using contour-clamped homogeneous electric field gel electrophoresis. Six chromosomal bands were separated with one migrating as a doublet. The size of the chromosomes was estimated to be between 3.8 and 6.0 megabase pairs (mb)...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351795
更新日期:1993-09-01 00:00:00
abstract::Meiotic fine-structure maps of two efficient UGA suppressors of Schizosaccharomyces pombe which are known (sup8-e) or inferred (sup10-e) to code for two leucine tRNAs carrying the mutant anticodon U(*)CA (Kohli et al. 1979, 1980a, b; Wetzel et al. 1979; Mao et al. 1981) are presented. In both cases, the recombination ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00365633
更新日期:1983-04-01 00:00:00
abstract::Cells of all living organisms contain complex signal transduction networks to ensure that a wide range of physiological properties are properly adapted to the environmental conditions. The fundamental concepts and individual building blocks of these signalling networks are generally well-conserved from yeast to man; y...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-009-0287-1
更新日期:2010-02-01 00:00:00
abstract::The unicellular green alga Chlamydomonas reinhardtii has emerged as a superb model species in plant biology. Although the alga is easily transformable, the low efficiency of transgene expression from the Chlamydomonas nuclear genome has severely hampered functional genomics research. For example, poor transgene expres...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-008-0189-7
更新日期:2008-06-01 00:00:00
abstract::The generation of a comprehensive EST library and the sequencing of its genome set the stage for reverse genetics approaches in Chlamydomonas reinhardtii. However, these also require tools for the specific downregulation of target gene expression. Consequently, a large number of diverse constructs were developed aimed...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-005-0042-1
更新日期:2006-02-01 00:00:00
abstract::In the multicellular red alga Antithamnion spec. both rubisco genes (rbcL and rbcS) are encoded on the plastid DNA (ptDNA). Both genes are separated by a short A/T-rich spacer of 100 bp and are cotranscribed into an mRNA of approximately 2.7 kb. These findings are in extensive agreement with those obtained from two un...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309918
更新日期:1990-12-01 00:00:00
abstract::Forces generated by molecular motors and the cytoskeleton move the nucleus and genome during many cellular processes, including cell migration and division. How these forces impact the genome, and whether cells regulate cytoskeletal forces to preserve genome integrity is unclear. We recently demonstrated that, in budd...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-019-01033-2
更新日期:2020-04-01 00:00:00
abstract::The Saccharomyces cerevisiae nuclear gene MRP-S9 was identified as part of the European effort in sequencing chromosome II. MRP-S9 encodes for a hydrophilic and basic protein of 278 amino acids with a molecular mass of 32 kDa. The C-terminal part (aa 153-278) of the MRP-S9 protein exhibits significant sequence similar...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00311878
更新日期:1995-06-01 00:00:00
abstract::The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00336850
更新日期:1992-03-01 00:00:00
abstract::Inorganic phosphate is an essential nutrient because it is required for the biosynthesis of nucleotides, phospholipids and metabolites in energy metabolism. During phosphate starvation, phosphatases play a major role in phosphate acquisition by hydrolyzing phosphorylated macromolecules. In Saccharomyces cerevisiae, PH...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-015-0544-4
更新日期:2016-05-01 00:00:00
abstract::The ribosomal proteins of two ascomycetes Podospora anserina and Saccharomyces cerevisiae, were compared by two dimensional electrophoresis in two different gel systems and we found only five pairs of proteins which have kept homologous physico-chemical properties under these conditions. An immunological analysis was ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00396204
更新日期:1984-12-01 00:00:00
abstract::For a comprehensive evaluation of genes that have their expression modulated during exposure of the mycelia to voriconazole, we performed a large-scale analysis of gene expression in Aspergillus fumigatus using a microarray hybridization approach. By comparing the expression of genes between the reference time and aft...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0073-2
更新日期:2006-07-01 00:00:00
abstract::A fine restriction map of the linear mitochondrial DNA of Tetrahymena pyriformis strain ST is presented. 1. Based on agarose gel electrophoresis data together with limited nucleotide sequences available on some restriction fragments, we estimate the actual size of this genome to be about 55,000 base pairs. 2. Seven tR...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00434053
更新日期:1985-01-01 00:00:00
abstract::The GGP1 gene encodes the only GPI-anchored glycoprotein (gp115) that has been purified to-date in the budding yeast Saccharomyces cerevisiae. It is a single-copy gene whose deduced amino-acid sequence shares no significant homology to any other known protein. In this paper we report a Southern hybridization analysis ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00336744
更新日期:1993-01-01 00:00:00
abstract::Schizosaccharomyces pombe Cdc23 is an essential DNA replication protein, conserved in eukaryotes and functionally homologous with Saccharomyces cerevisiae Dna43 (Mcm10). We sought evidence for interactions between Cdc23 and the MCM2-7 complex, a component of both the pre-replicative complex and the replication fork. C...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-002-0316-9
更新日期:2002-08-01 00:00:00
abstract::Chloroplast DNA from the fern Osmunda einnamomea was isolated by a sucrose gradient procedure utilizing PEG to stabilize chloroplasts. Analysis with the restriction endonucleases PvuII, Sacl and BstEII indicates a chloroplast genome size of 144 kb. A physical map of the fragments produced by these three enzymes was co...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00391801
更新日期:1982-08-01 00:00:00
abstract::cAMP-dependent protein kinases (PKA) are ubiquitous signalling molecules that mediate many extracellular signals in eukaryotes from yeast to men. Directing PKA to its substrates is an important level of control to ensure specificity of cAMP-mediated signal transduction. Unlike in yeast and fungi, in mammalian cells a ...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-002-0308-9
更新日期:2002-07-01 00:00:00
abstract::The complete nucleotide sequence of one of three linear DNA plasmids (pRS64-2) from the plant pathogenic fungus Rhizoctonia solani was determined. The pRS64-2 DNA consisted of 2877 nucleotides. The nucleotide sequences of the middle 2.2-kb regions of the other two plasmids (pRS64-1 and pRS64-3) were also determined. C...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050299
更新日期:1997-12-01 00:00:00
abstract::We have determined the nucleotide sequence of a region of the mitochondrial genome of the yeast Saccharomyces douglasii which contains the ATPase subunit 9 gene and part of the intergenic sequences that surround it. The gene is 228 nucleotides long and encodes a polypeptide of 76 aa. A comparison of the coding sequenc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351669
更新日期:1994-06-01 00:00:00
abstract::The steady-state transcript levels for psbA, psbD, psaA and rbcL are low in dark-grown rice seedlings as compared to those grown in light. Following seed germination, they accumulate in an age-dependent manner, in dark as well as light, reaching a maximal level on the 7th or 8th day, before a slow decline sets in. But...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351491
更新日期:1994-04-01 00:00:00
abstract::Phyto-pathogenic fungi can cause huge damage to crop production. During millions of years of coexistence, fungi have evolved diverse life-style to obtain nutrients from the host and to colonize upon them. They deploy various proteinaceous as well as non-proteinaceous secreted molecules commonly referred as effectors t...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-020-01118-3
更新日期:2020-11-04 00:00:00
abstract::Analysis of the cDNA of the atp9-1 gene transcript from petunia mitochondria has revealed that ten C residues of the gene sequence are edited into U in the mRNA. Seven of these edits result in amino acid changes and one introduces a stop codon before the end of the open reading frame predicted from the gene sequence. ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00362089
更新日期:1991-01-01 00:00:00
abstract::Spindle pole body (SPB; the microtubule organizing center in yeast) duplication is essential to form a bipolar spindle. The duplicated SPBs must then separate and migrate to opposite sides of the nucleus. We identified a novel functional relationship in SPB separation between the microtubule stabilizing protein Bik1p/...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0098-6
更新日期:2006-12-01 00:00:00
abstract::Work in recent years has produced indirect evidence to support the view that the phenomenon of suppressiveness in yeast is the result of the ability of the petite mtDNA to out-replicate the wild-type genome. We have developed a method, based on fluorography of gels containing restriction fragments of radioactively lab...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00418122
更新日期:1986-01-01 00:00:00
abstract::The PEL1 gene of Saccharomyces cerevisiae is essential for the cell viability of mitochondrial petite mutants, for the ability to utilize glycerol and ethanol on synthetic medium, and for cell growth at higher temperatures. By tetrad analysis the gene was assigned to chromosome III, centromere proximal of LEU2. The PE...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00336781
更新日期:1993-10-01 00:00:00
abstract::In Saccharomyces cerevisiae, the HAP transcriptional complex is involved in the fermentation-respiration shift. This complex is composed of four subunits. Three subunits are necessary for DNA-binding, whereas the Hap4p subunit, glucose-repressed, contains the transcriptional activation domain. Hap4p is the key regulat...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-004-0556-y
更新日期:2005-03-01 00:00:00
abstract::Cellulases belong to the large family of glycosyl hydrolases (GHs) and are produced by a variety of bacteria and fungi. These extracellular enzymes act as endoglucanases (EGs), cellobiohydrolases or beta-glucosidases. In this paper, we describe molecular screening for EGs from the GH family 12. Using three homologous ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-002-0290-2
更新日期:2002-05-01 00:00:00