Abstract:
:The GGP1 gene encodes the only GPI-anchored glycoprotein (gp115) that has been purified to-date in the budding yeast Saccharomyces cerevisiae. It is a single-copy gene whose deduced amino-acid sequence shares no significant homology to any other known protein. In this paper we report a Southern hybridization analysis of genomic DNA from different eukaryotic organisms to identify homologues of the GGP1 gene. We have analyzed DNA prepared from a unicellular green alga (Chlamydomonas eugametos), from two distantly related yeast species (Candida cylindracea and Schizosaccharomyces pombe), and from the common bean Phasoleus vulgaris. The moderate stringency of the experimental conditions and the high specificity of the probes used indicate that a single-copy of GGP1-related sequences exists in all these eukaryotic organisms. The chromosomal localization of the GGP1 gene in S. cerevisiae has also been determined.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Vai M,Lacanà E,Gatti E,Breviario D,Popolo L,Alberghina Ldoi
10.1007/BF00336744subject
Has Abstractpub_date
1993-01-01 00:00:00pages
19-21issue
1eissn
0172-8083issn
1432-0983journal_volume
23pub_type
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