Cloning and expression of the OMP decarboxylase gene URA4 from Schizosaccharomyces pombe.

abstract：:Schizosaccharomyces pombe Cdc23 is an essential DNA replication protein, conserved in eukaryotes and functionally homologous with Saccharomyces cerevisiae Dna43 (Mcm10). We sought evidence for interactions between Cdc23 and the MCM2-7 complex, a component of both the pre-replicative complex and the replication fork. C...

journal_title：Current genetics

authors： Hart EA,Bryant JA,Moore K,Aves SJ

更新日期：2002-08-01 00:00:00

abstract：:Enzymatic and genetic characterization of cpd-1 and cpd-2, which exhibit rhythmic conidiation in liquid media and on solid media, were described with band (bd) strain as a reference. Cpd-1 and cpd-2 showed reduced growth in orthophosphate-free cyclic 3',5'-AMP media, while bd showed wild-type level of growth in the me...

journal_title：Current genetics

authors： Hasunuma K,Shinohara Y

更新日期：1985-01-01 00:00:00

abstract：:The zinc finger transcription factor Crz1 is an important downstream regulator of calcium-dependent signal transduction pathways in many organisms. The function of Crz1 in the wheat-head blight pathogen Fusarium graminearum remains unclear. In this study, we identified and functionally characterised FgCrz1A, a potenti...

journal_title：Current genetics

authors： Chen L,Tong Q,Zhang C,Ding K

更新日期：2019-02-01 00:00:00

abstract：:We have determined the nucleotide sequence of a region of the mitochondrial genome of the yeast Saccharomyces douglasii which contains the ATPase subunit 9 gene and part of the intergenic sequences that surround it. The gene is 228 nucleotides long and encodes a polypeptide of 76 aa. A comparison of the coding sequenc...

journal_title：Current genetics

authors： Nicoletti L,Laveder P,Pellizzari R,Cardazzo B,Carignani G

更新日期：1994-06-01 00:00:00

abstract：:Spindle pole body (SPB; the microtubule organizing center in yeast) duplication is essential to form a bipolar spindle. The duplicated SPBs must then separate and migrate to opposite sides of the nucleus. We identified a novel functional relationship in SPB separation between the microtubule stabilizing protein Bik1p/...

journal_title：Current genetics

authors： Strawn LA,True HL

更新日期：2006-12-01 00:00:00

abstract：:Unlike most polymorphic markers in the Chlamydomonas eugametos and Chlamydomonas moewusii chloroplast DNAs (cpDNAs), the C. moewusii 6- and 21-kb extra sequences and the C. eugametos-specific CeLSU small middle dot 5 intron are transmitted to all of the few viable progeny in reciprocal crosses between the two green al...

journal_title：Current genetics

authors： Bussières J,Lemieux C,Lee RW,Turmel M

更新日期：1996-09-01 00:00:00

abstract：:In yeast (S. cerevisiae) the stringent response is known to include rapid, selective, and severe transcriptional curtailment for genes specifying cytoplasmic rRNAs and r-proteins. We have shown that transcription of the mitochondrial 21S rRNA gene is also congruently and selectively curtailed during the yeast stringen...

journal_title：Current genetics

authors： Cantwell R,McEntee CM,Hudson AP

更新日期：1992-03-01 00:00:00

abstract：:We have used a PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), to assess genome variability between 21 isolates from F. solani f. sp. cucurbitae races 1 and 2. Based on RAPD marker patterns the isolates fell into two distinct groups corresponding to mating populations MPI and MPV. Fo...

journal_title：Current genetics

authors： Crowhurst RN,Hawthorne BT,Rikkerink EH,Templeton MD

更新日期：1991-11-01 00:00:00

abstract：:In the red alga Gracilaria verrucosa, the genes encoding the large and the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) are separated by a short spacer of less than 131 bp. Sequencing of PCR-amplified Rubisco spacers from a number of populations of G. verrucosa was performed to assess the...

journal_title：Current genetics

authors： Destombe C,Douglas SE

更新日期：1991-05-01 00:00:00

abstract：:La France disease of the cultivated mushroom, Agaricus bisporus, is known to be associated with the presence of a number of dsRNA segments. The nucleotide sequences of the dsRNAs M2 (1.3 kb), M1 (1.55 kb) and L3 (2.8 kb), invariably associated with the disease, were determined. Putative coding sequences for proteins w...

journal_title：Current genetics

authors： Harmsen MC,Tolner B,Kram A,Go SJ,de Haan A,Wessels JG

更新日期：1991-07-01 00:00:00

abstract：:DNA-hybridisation studies showed a close relationship between Phanerochaete chrysosporium ME446, most used in lignin degradation studies, and Sporotrichum pulverulentum Novobranova, the other standard lignin degrading strain. Two other strains of P. chrysosporium were both less related. We show that P. chrysosporium M...

journal_title：Current genetics

authors： Raeder U,Broda P

更新日期：1984-09-01 00:00:00

abstract：:ERG24 is the structural gene for the C14-sterol reductase in yeast. A lack of activity in that enzyme, mediated either by the morpholine fungicides or the insertional inactivation of ERG24, causes the accumulation of the aberrant sterol ignosterol. Cells producing this sterol are unable to grow aerobically in the rout...

journal_title：Current genetics

authors： Crowley JH,Tove S,Parks LW

更新日期：1998-08-01 00:00:00

abstract：:The filamentous/invasive growth pathway is activated by nutrient limitation in the haploid form of the yeast Saccharomyces cerevisiae, whereas exposure to mating-pheromone causes cells to differentiate into gametes. Although these two pathways respond to very different stimuli and generate very different responses, th...

journal_title：Current genetics

authors： Draper E,Dubrovskyi O,Bar EE,Stone DE

更新日期：2009-12-01 00:00:00

abstract：:The complete nucleotide sequence of the Morus mongolica chloroplast (cp) genome was reported and characterized in this study. The cp genome is a circular molecule of 158,459 bp containing a pair of 25,678 bp IR regions, separated by small and large single-copy regions of 19,736 and 87,363 bp, respectively. The number ...

journal_title：Current genetics

authors： Kong W,Yang J

更新日期：2016-02-01 00:00:00

abstract：:Thirteen chromosomal-sized DNA bands of the cultivated mushroom Agaricus bisporus have been resolved using the method of clamped homogeneous electric field (CHEF) electrophoresis. Using chromosome size standards from Schizosaccharomyces pombe, Saccharomyces cerevisiae and Candida albicans, the estimated size of the ch...

journal_title：Current genetics

authors： Lodder S,Gull K,Wood D

更新日期：1993-12-01 00:00:00

abstract：:Retrotransposons are activated as organisms age, based on work from several model systems. Since these mobile DNA elements can promote genome instability, this has raised the possibility that they can contribute to loss of cellular function with age. Many questions remain to be addressed about the relationship between...

journal_title：Current genetics

authors： Maxwell PH

更新日期：2016-05-01 00:00:00

abstract：:The single-copy nuclear gene (GapA), encoding the plastid-localized glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of the marine red alga Gracilaria verrucosa, has been cloned and sequenced. The GapA transcriptional initiation site was located 49 bp upstream of the start codon, and a putative TATA box was found 54 b...

journal_title：Current genetics

authors： Zhou YH,Ragan MA

更新日期：1994-07-01 00:00:00

abstract：:The var1 locus on yeast mitochondria) DNA is defined by a set of alleles that affect the apparent size (Mr 40,000-44,000) of a mitochondria) translation product (var1), a protein of the mitochondria) 38S ribosomal subunit. Using petite deletion and restriction endonuclease site mapping, we have defined the physical lo...

journal_title：Current genetics

authors： Vincent RD,Perlman PS,Strausberg RL,Butow RA

更新日期：1980-07-01 00:00:00

abstract：:In Aspergillus nidulans, carbon catabolite repression (CCR) is mediated by the global repressor protein CreA. The deubiquitinating enzyme CreB is a component of the CCR network. Genetic interaction was confirmed using a strain containing complete loss-of-function alleles of both creA and creB. No direct physical inter...

journal_title：Current genetics

authors： Alam MA,Kelly JM

更新日期：2017-08-01 00:00:00

abstract：:Gene expression using alternative transcription start sites (TSSs) is an important transcriptional regulatory mechanism for environmental responses in eukaryotes. Here, we identify two alternative TSSs in the enolase-encoding gene (enoA) in Aspergillus oryzae, an industrially important filamentous fungus. TSS use in e...

journal_title：Current genetics

authors： Inoue T,Toji H,Tanaka M,Takama M,Hasegawa-Shiro S,Yamaki Y,Shintani T,Gomi K

更新日期：2020-08-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the HAP transcriptional complex is involved in the fermentation-respiration shift. This complex is composed of four subunits. Three subunits are necessary for DNA-binding, whereas the Hap4p subunit, glucose-repressed, contains the transcriptional activation domain. Hap4p is the key regulat...

journal_title：Current genetics

authors： Sybirna K,Guiard B,Li YF,Bao WG,Bolotin-Fukuhara M,Delahodde A

更新日期：2005-03-01 00:00:00

abstract：:Chloroplast DNA from the fern Osmunda einnamomea was isolated by a sucrose gradient procedure utilizing PEG to stabilize chloroplasts. Analysis with the restriction endonucleases PvuII, Sacl and BstEII indicates a chloroplast genome size of 144 kb. A physical map of the fragments produced by these three enzymes was co...

journal_title：Current genetics

authors： Palmer JD,Stein DB

更新日期：1982-08-01 00:00:00

abstract：:The isoprenoid pathway of the ectomycorrhizal fungus Tuber borchii Vittad is investigated to better understand the molecular mechanisms at work, in particular during the maturation of the complex ascomata (the so-called "truffles"). Three T. borchii genes coding for the most important regulatory enzymes of the isopren...

journal_title：Current genetics

authors： Guidi C,Zeppa S,Annibalini G,Pierleoni R,Guescini M,Buffalini M,Zambonelli A,Stocchi V

更新日期：2006-12-01 00:00:00

abstract：:Tuplp is a general corepressor in Saccharomyces cerevisiae. We performed a split-ubiquitin screen with Tup1p as bait, in order to understand how Tuplp mediates repression. Cdc73p was the only component of a holoenzyme of transcription isolated as a Tuplp-interacting protein in this screen. Expression analysis of a str...

journal_title：Current genetics

authors： Kerkmann K,Lehming N

更新日期：2001-07-01 00:00:00

abstract：:In Chlamydomonas reinhardtii, one displacement loop region which initiates the replication of chloroplast DNA was located on a 1.05 kb restriction fragment. This fragment was cloned and sequenced. In this report, the galK expression plasmid, pKO1 was used to screen for the presence of any prokaryotic promoter within t...

journal_title：Current genetics

authors： Wu M,Kong XF,Kung SD

更新日期：1986-01-01 00:00:00

abstract：:Two cob- deletion mutants are characterized. One of them, M9410, is deleted for 911 bp of the noncoding sequences only which separate tRNAGlu and cob exon 1; it thus lacks most of the sequence encoding the 957 bp long cob leader (Bonitz et al. 1982) and some 20 bp 5' to it. The end points of this deletion coincide wit...

journal_title：Current genetics

authors： Dobres M,Gerbl-Rieger S,Schmelzer C,Mueller MW,Schweyen RJ

更新日期：1985-01-01 00:00:00

abstract：:We have characterised a cDNA clone and a nuclear gene encoding the chloroplast 30 s ribosomal protein S13 from Arabidopsis thaliana. The identification is based on the high similarity of the predicted amino-acid sequence with eubacterial S13 protein sequences, and immunodetection of a 14.5-kDa chloroplast ribosomal po...

journal_title：Current genetics

authors： Kumar R,Drouaud J,Raynal M,Small I

更新日期：1995-09-01 00:00:00

abstract：:A 1,380-bp intervening sequence within the mitochondrial small subunit ribosomal RNA (mt SSU rRNA) gene of the fungus Sclerotinia sclerotiorum has been sequenced and identified as a group-I intron. This is the first report of an intron in the mt SSU rRNA gene. The intron shows close similarity in secondary structure t...

journal_title：Current genetics

authors： Carbone I,Anderson JB,Kohn LM

更新日期：1995-01-01 00:00:00

abstract：:In Chlamydomonas reinhardtii, the expression of the nit1 gene encoding nitrate reductase is dependent on the nature of the nitrogen source and on other environmental factors. We have fused the nit1 promoter region to the arylsulphatase (ars) reporter gene lacking its own promoter and introduced this chimeric construct...

journal_title：Current genetics

authors： Ohresser M,Matagne RF,Loppes R

更新日期：1997-03-01 00:00:00

abstract：:Wild-type Neurospora crassa, strain Singapore, was transformed with a N. crassa metallothionein promoter/protyrosinase fusion gene. Transformants produced tyrosinase during vegetative growth, as determined by Western analyses and activity assays. This is in sharp contrast to wild-type strains, where this enzyme is onl...

journal_title：Current genetics

authors： Kupper U,Linden M,Cao KZ,Lerch K

更新日期：1990-11-01 00:00:00