Cloning and characterization of the nuclear gene encoding plastid glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa.

Abstract:

:The single-copy nuclear gene (GapA), encoding the plastid-localized glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of the marine red alga Gracilaria verrucosa, has been cloned and sequenced. The GapA transcriptional initiation site was located 49 bp upstream of the start codon, and a putative TATA box was found 54 bp farther upstream. A spliceosomal intron was identified in the transit-peptide-encoding region in a position very similar to intron 1 of GapA and GapB of higher plants; no introns occur in the region encoding the mature protein. These observations provisionally suggest that both red algae and higher plants descend from a single ancestral photosynthetic eukaryote, i.e. that a single endosymbiotic event gave rise to red algal and higher-plant plastids.

journal_name

Curr Genet

journal_title

Current genetics

authors

Zhou YH,Ragan MA

doi

10.1007/BF00326308

subject

Has Abstract

pub_date

1994-07-01 00:00:00

pages

79-86

issue

1

eissn

0172-8083

issn

1432-0983

journal_volume

26

pub_type

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