Abstract:
:We have determined the nucleotide sequence of a region of the mitochondrial genome of the yeast Saccharomyces douglasii which contains the ATPase subunit 9 gene and part of the intergenic sequences that surround it. The gene is 228 nucleotides long and encodes a polypeptide of 76 aa. A comparison of the coding sequence with that of S. cerevisiae reveals the presence of three silent transitions. A high level of similarity is also found between regions involved in the initiation of transcription and mRNA processing. More interestingly, a region of similarity situated outside the known regulatory regions has been identified. As the intergenic regions are generally highly divergent, the remarkable conservation of these non-coding sequences suggests that their structure may be relevant to the expression of this region of the mitochondrial DNA.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Nicoletti L,Laveder P,Pellizzari R,Cardazzo B,Carignani Gdoi
10.1007/BF00351669subject
Has Abstractpub_date
1994-06-01 00:00:00pages
504-7issue
6eissn
0172-8083issn
1432-0983journal_volume
25pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::The mitochondrial leucyl-tRNA synthetase (mLRS) of Saccharomyces cerevisiae is involved in both mitochondrial protein synthesis and pre-mRNA splicing. We have created mutations in the regions HIGH, GWD and KMSKS, which are involved in ATP-, amino acid- and tRNA-binding respectively, and which have been conserved in th...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351744
更新日期:1992-07-01 00:00:00
abstract::Mutants which carry a leaky UGA nonsense mutation in the fertility gene ste10 are characterized by a deformed cell morphology which resembles that described in the literature for sterile ras1- (ste5) and ral1 to ral4 mutant cells. Although frequent conjugation attempts are observed in combinations of two ste10 mutant ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00312768
更新日期:1991-07-01 00:00:00
abstract::The PEL1 gene of Saccharomyces cerevisiae is essential for the cell viability of mitochondrial petite mutants, for the ability to utilize glycerol and ethanol on synthetic medium, and for cell growth at higher temperatures. By tetrad analysis the gene was assigned to chromosome III, centromere proximal of LEU2. The PE...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00336781
更新日期:1993-10-01 00:00:00
abstract::How group I introns originate in nuclear ribosomal (r)RNA genes is an important question in evolutionary biology. Central to this issue is the multitude of group I introns present in evolutionarily distantly related plant, fungal, and protist lineages, together with an understanding of their origin and lateral transfe...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940100227
更新日期:2001-08-01 00:00:00
abstract::DNA-hybridisation studies showed a close relationship between Phanerochaete chrysosporium ME446, most used in lignin degradation studies, and Sporotrichum pulverulentum Novobranova, the other standard lignin degrading strain. Two other strains of P. chrysosporium were both less related. We show that P. chrysosporium M...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00410436
更新日期:1984-09-01 00:00:00
abstract::Glutathione (GSH) is an abundantly produced low-molecular-weight (LMW) thiol in many organisms. However, a number of Gram-positive bacteria do not produce GSH, but instead produce bacillithiol (BSH) as one of the major LMW thiols. Similar to GSH, studies have found that BSH has various roles in the cell, including pro...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-015-0511-0
更新日期:2016-02-01 00:00:00
abstract::Schizosaccharomyces pombe Cdc23 is an essential DNA replication protein, conserved in eukaryotes and functionally homologous with Saccharomyces cerevisiae Dna43 (Mcm10). We sought evidence for interactions between Cdc23 and the MCM2-7 complex, a component of both the pre-replicative complex and the replication fork. C...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-002-0316-9
更新日期:2002-08-01 00:00:00
abstract::NRE, the nitrogen regulatory protein of Penicillium chrysogenum, contains a single Cys2/Cys2-type zinc-finger motif followed immediately by a highly basic region. The zinc-finger domain was expressed to Escherichia coli as a fusion protein with beta-galactosidase. In order to test the putative DNA-binding ability of N...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00315785
更新日期:1995-07-01 00:00:00
abstract::Mitotic homologous recombination (HR) is an important mechanism for the repair of double-strand breakS and errors occurring during DNA replication. It is likely that the recombinational repair of DNA lesions occurs preferentially by sister chromatid exchanges that have no genetic consequences. However, most geneticall...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-002-0346-3
更新日期:2003-01-01 00:00:00
abstract::Phyto-pathogenic fungi can cause huge damage to crop production. During millions of years of coexistence, fungi have evolved diverse life-style to obtain nutrients from the host and to colonize upon them. They deploy various proteinaceous as well as non-proteinaceous secreted molecules commonly referred as effectors t...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-020-01118-3
更新日期:2020-11-04 00:00:00
abstract::Nucleic acid extracted from killer strains of Saccharomyces cerevisiae and run on agarose gels under sub-optimal conditions for M dsRNA yielded two species of L dsRNA (L1 and L2) which migrated at slightly different rates. L2 dsRNA was shown to be dependent upon MAK3, MAK10 and PES1 but not MAK1 nuclear genes for its ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00365682
更新日期:1983-03-01 00:00:00
abstract::Thirteen chromosomal-sized DNA bands of the cultivated mushroom Agaricus bisporus have been resolved using the method of clamped homogeneous electric field (CHEF) electrophoresis. Using chromosome size standards from Schizosaccharomyces pombe, Saccharomyces cerevisiae and Candida albicans, the estimated size of the ch...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351712
更新日期:1993-12-01 00:00:00
abstract::Chloroplast genes are typically organized into polycistronic transcription units that give rise to complex sets of mono- and oligo-cistronic overlapping RNAs through a series of processing steps. The psbB operon contains genes for the PSII (psbB, psbT, psbH) and cytochrome b(6)f (petB and petD) complexes which are nee...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050487
更新日期:1999-09-01 00:00:00
abstract::By functional complementation of the auxotrophic requirements for choline of a cdg1, cho2 double-mutant, by transformation with a genomic DNA library in a high copy number plasmid, two different types of complementing DNA inserts were identified. One type of insert was earlier shown to represent the CHO2 structural ge...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00352006
更新日期:1993-02-01 00:00:00
abstract::The meiotic recombination gene rec10, which encodes a region-specific activator of recombination in Schizosaccharomyces pombe, has been cloned by genetic complementation and its nucleotide sequence determined. The rec10 gene was identified in a 5.6-kb cloned fragment by partial-deletion and insertion experiments. The ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00311213
更新日期:1995-04-01 00:00:00
abstract::By using mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) as genetic markers, the modes of mitochondrial inheritance in sexual crosses and protoplast cell fusions of the higher basidiomycete Lentinula edodes were examined. All newly established dikaryons from reciprocal crosses between compa...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00314446
更新日期:1995-05-01 00:00:00
abstract::Cellular reverse transcriptase-related (rvt) genes represent a novel class of reverse transcriptases (RTs), which are only distantly related to RTs of retrotransposons and retroviruses, but, similarly to telomerase RTs, are immobilized in the genome as single-copy genes. They have been preserved by natural selection t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-018-0844-6
更新日期:2018-12-01 00:00:00
abstract::International efforts are underway that aim at determining the complete genome sequence of the social amoeba Dictyostelium discoideum. As strategy, a whole chromosome shotgun (WCS) approach was chosen and each of the six Dictyostelium chromosomes was assigned to project partners. The project is well advanced, chromoso...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-003-0416-1
更新日期:2003-11-01 00:00:00
abstract::The S. cerevisiae strain h56 is a temperature-sensitive mit- mutant containing a single nucleotide substitution in the region 5' to the reading frame of the mitochondrial var1 gene. The mutation decreases the efficiency of processing of a precursor RNA such that little var1 mRNA is produced at the restrictive temperat...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00357168
更新日期:1994-03-01 00:00:00
abstract::Studies on replicative and chronological aging in Saccharomyces cerevisiae have greatly advanced our understanding of how longevity is regulated in all eukaryotes. Chronological lifespan (CLS) of yeast is defined as the age-dependent viability of non-dividing cell populations. A number of nutrient sensing and signal t...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-017-0697-4
更新日期:2017-10-01 00:00:00
abstract::In Chlamydomonas reinhardtii, one displacement loop region which initiates the replication of chloroplast DNA was located on a 1.05 kb restriction fragment. This fragment was cloned and sequenced. In this report, the galK expression plasmid, pKO1 was used to screen for the presence of any prokaryotic promoter within t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00418528
更新日期:1986-01-01 00:00:00
abstract::The wheat mitochondrial (mt) cox3 has been localized and sequenced. The gene exists as a single copy in the wheat mt master chromosome and is transcribed into a single 1.2 kb RNA, whose extremities have been mapped. Comparison of the wheat and Oenothera cox3 sequences gives ambiguous indications concerning the amino a...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00313247
更新日期:1990-01-01 00:00:00
abstract::The sequence of a representative mitochondrial gene COXI, encoding cytochrome c oxidase subunit I, was determined in five species that cover all the orders of the Prymnesiophyta with the exception of the Pavlovales. Through this analysis, we noticed that the 'stop' codon UGA appears frequently and, specifically, at co...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050280
更新日期:1997-10-01 00:00:00
abstract::For a comprehensive evaluation of genes that have their expression modulated during exposure of the mycelia to voriconazole, we performed a large-scale analysis of gene expression in Aspergillus fumigatus using a microarray hybridization approach. By comparing the expression of genes between the reference time and aft...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0073-2
更新日期:2006-07-01 00:00:00
abstract::Unlike most polymorphic markers in the Chlamydomonas eugametos and Chlamydomonas moewusii chloroplast DNAs (cpDNAs), the C. moewusii 6- and 21-kb extra sequences and the C. eugametos-specific CeLSU small middle dot 5 intron are transmitted to all of the few viable progeny in reciprocal crosses between the two green al...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050144
更新日期:1996-09-01 00:00:00
abstract::The ornithine transaminase (otaA) gene of Aspergillus nidulans has been cloned by transformation of the A. nidulans pro-ota- mutant strain with a cosmid gene library. The otaA gene contains two introns and potentially codes for a 453-aa-long protein. The deduced amino-acid sequence is homologous to known ornithine tra...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050440
更新日期:1999-03-01 00:00:00
abstract::Mutations in the RecA-like genes RAD51 and RAD57 reduce the frequency of gene conversion/reciprocal exchange between inverted repeats 7-fold. However, they enhance the frequency of deletions between direct repeats 5-12-fold. These induced deletions are RAD1- and RAD52-dependent. On the basis of these results it is pro...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00352096
更新日期:1995-03-01 00:00:00
abstract::The amino acid sequences of two barley ribosomal proteins, termed HvL17-1 and HvL17-2, were decoded from green leaf cDNA clones. The N-terminal sequences of the derived barley proteins are 48% identical to the N-terminal amino acid sequence of protein YL17 from the large subunit of yeast cytoplasmic ribosomes. Via arc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309605
更新日期:1991-05-01 00:00:00
abstract::The mating-type ( MAT) genes from Rhynchosporium secalis were isolated using PCR-based methods. Characterisation of the MAT idiomorphs suggests that R. secalis is closely related to the discomycetes Pyrenopeziza brassicae and Tapesia yallundae in terms of sequence and MAT locus gene composition. The MAT1-2 idiomorph c...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-003-0445-9
更新日期:2003-12-01 00:00:00
abstract::To understand the organization of the mating type locus of Stagonosporopsis tanaceti and Stagonosporopsis chrysanthemi, and its potential role in the epidemiology of ray blight of pyrethrum and chrysanthemum, respectively, the mating type (MAT) locus of these species was cloned and characterized using PCR-based techni...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-014-0435-0
更新日期:2014-11-01 00:00:00