Genome-wide expression analysis of a Saccharomyces cerevisiae strain deleted for the Tup1p-interacting protein Cdc73p.

Abstract:

:Tuplp is a general corepressor in Saccharomyces cerevisiae. We performed a split-ubiquitin screen with Tup1p as bait, in order to understand how Tuplp mediates repression. Cdc73p was the only component of a holoenzyme of transcription isolated as a Tuplp-interacting protein in this screen. Expression analysis of a strain deleted for CDC73 on a genome-wide scale revealed that Cdc73p is involved in the positive and negative regulation of transcription. Our results show that a subset of genes is regulated by both Cdc73p and Tup1p. Furthermore, our results indicate that Cdc73p plays a role in the repression of telomere-proximal genes, which are not repressed by Tup1p.

journal_name

Curr Genet

journal_title

Current genetics

authors

Kerkmann K,Lehming N

doi

10.1007/s002940100229

subject

Has Abstract

pub_date

2001-07-01 00:00:00

pages

284-90

issue

5-6

eissn

0172-8083

issn

1432-0983

journal_volume

39

pub_type

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