Abstract:
:ERG24 is the structural gene for the C14-sterol reductase in yeast. A lack of activity in that enzyme, mediated either by the morpholine fungicides or the insertional inactivation of ERG24, causes the accumulation of the aberrant sterol ignosterol. Cells producing this sterol are unable to grow aerobically in the routine laboratory medium, YPD. However, growth does occur on a synthetic defined medium. A novel calcium-dependent phenotype associated with alterations in the ergosterol biosynthetic pathway in yeast is described. In addition, reduction of yeast growth with an azole inhibitor of the C-14 sterol de-methylase was also modulated by an excess of calcium ions in the culture medium. These results define a new effect of ergosterol deficiency and provide important practical implications for utilizing morpholine and azole sterol biosynthetic-inhibiting fungicides.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Crowley JH,Tove S,Parks LWdoi
10.1007/s002940050371subject
Has Abstractpub_date
1998-08-01 00:00:00pages
93-9issue
2eissn
0172-8083issn
1432-0983journal_volume
34pub_type
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