A calcium-dependent ergosterol mutant of Saccharomyces cerevisiae.

Abstract:

:ERG24 is the structural gene for the C14-sterol reductase in yeast. A lack of activity in that enzyme, mediated either by the morpholine fungicides or the insertional inactivation of ERG24, causes the accumulation of the aberrant sterol ignosterol. Cells producing this sterol are unable to grow aerobically in the routine laboratory medium, YPD. However, growth does occur on a synthetic defined medium. A novel calcium-dependent phenotype associated with alterations in the ergosterol biosynthetic pathway in yeast is described. In addition, reduction of yeast growth with an azole inhibitor of the C-14 sterol de-methylase was also modulated by an excess of calcium ions in the culture medium. These results define a new effect of ergosterol deficiency and provide important practical implications for utilizing morpholine and azole sterol biosynthetic-inhibiting fungicides.

journal_name

Curr Genet

journal_title

Current genetics

authors

Crowley JH,Tove S,Parks LW

doi

10.1007/s002940050371

subject

Has Abstract

pub_date

1998-08-01 00:00:00

pages

93-9

issue

2

eissn

0172-8083

issn

1432-0983

journal_volume

34

pub_type

杂志文章
  • Genetic effects of photoactivated psoralens during meiosis in DNA repair mutant pso3-1 of Saccharomyces cerevisiae.

    abstract::The influence of the DNA repair gene PSO3 on photoactivated psoralen-induced meiotic recombination, gene conversion, reverse mutation, and on survival, was assayed in diploid strains of Saccharomyces cerevisiae homozygous for the wild-type or the pso3-1 mutant allele. Sporulation was normal in the pso3-1 diploid. Wild...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00712961

    authors: Pothin HS,da Silva KV,Brendel M,Henriques JA

    更新日期:1994-01-01 00:00:00

  • psbD sequences of Bumilleriopsis filiformis (Heterokontophyta, Xanthophyceae) and Porphyridium purpureum (Rhodophyta, Bangiophycidae): evidence for polyphyletic origins of plastids.

    abstract::The nucleotide sequences of the plastidal psbD genes of Bumilleriopsis filiformis and Porphyridium purpureum (encoding the D2 protein of photosystem II) are reported in this paper. The Bumilleriopsis sequence clusters together with Porphyridium when a most parsimonious protein tree of D2 sequences is constructed. A co...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00351854

    authors: Scherer S,Lechner S,Böger P

    更新日期:1993-11-01 00:00:00

  • Cytochrome c 1-deficient mutants in Saccharomyces cerevisiae.

    abstract::1. Nuclear mutants lacking cytochrome c l spectrum have been isolated. 2. The electron flow through the respiratory chain of the mutants is defect in the cytochrome bc 1-segment. 3. Complementation analysis has yielded two major complementation groups. 4. Tetrade analysis has established the two complementation groups...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00390343

    authors: Lang BF,Kaudewitz F

    更新日期:1982-12-01 00:00:00

  • Expression and molecular properties of a new laccase of the white rot fungus Phlebia radiata grown on wood.

    abstract::Laccases are phenol-oxidizing, multicopper enzymes produced by fungi, plants, insects and bacteria. Fungal laccases are involved in ecologically important processes such as decomposition of lignocellulose (wood and plant material). In this work, in order to find out the role of fungal laccases upon wood colonisation a...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-006-0090-1

    authors: Mäkelä MR,Hildén KS,Hakala TK,Hatakka A,Lundell TK

    更新日期:2006-11-01 00:00:00

  • Genetic evidence for different RAD52-dependent intrachromosomal recombination pathways in Saccharomyces cerevisiae.

    abstract::Mutations in the RecA-like genes RAD51 and RAD57 reduce the frequency of gene conversion/reciprocal exchange between inverted repeats 7-fold. However, they enhance the frequency of deletions between direct repeats 5-12-fold. These induced deletions are RAD1- and RAD52-dependent. On the basis of these results it is pro...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00352096

    authors: Aguilera A

    更新日期:1995-03-01 00:00:00

  • Forever young: the key to rejuvenation during gametogenesis.

    abstract::Cell aging is the result of deteriorating competence in maintaining cellular homeostasis and quality control. Certain cell types are able to rejuvenate through asymmetric cell division by excluding aging factors, including damaged cellular compartments and extrachromosomal rDNA circles, from entering the daughter cell...

    journal_title:Current genetics

    pub_type: 杂志文章,评审

    doi:10.1007/s00294-020-01133-4

    authors: Koch-Bojalad BA,Carson L,Yu HG

    更新日期:2020-11-27 00:00:00

  • Gene fragmentation: a key to mitochondrial genome evolution in Euglenozoa?

    abstract::Phylum Euglenozoa comprises three groups of eukaryotic microbes (kinetoplastids, diplonemids, and euglenids), the mitochondrial (mt) genomes of which exhibit radically different modes of organization and expression. Gene fragmentation is a striking feature of both euglenid and diplonemid mtDNAs. To rationalize the eme...

    journal_title:Current genetics

    pub_type: 杂志文章,评审

    doi:10.1007/s00294-011-0340-8

    authors: Flegontov P,Gray MW,Burger G,Lukeš J

    更新日期:2011-08-01 00:00:00

  • Mutation of a putative AMPK phosphorylation site abolishes the repressor activity but not the nuclear targeting of the fungal glucose regulator CRE1.

    abstract::In filamentous ascomycetes, glucose repression is mediated by CRE1, a zinc-finger protein related to Miglp from yeast. Five putative AMPK phosphorylation motifs identified in the glucose repressor from the phytopathogenic fungus Sclerotinia sclerotiorum were mutated in a GFP::CRE1 translational fusion. Complementation...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940050535

    authors: Vautard-Mey G,Fèvre M

    更新日期:2000-05-01 00:00:00

  • Dse1 may control cross talk between the pheromone and filamentation pathways in yeast.

    abstract::The filamentous/invasive growth pathway is activated by nutrient limitation in the haploid form of the yeast Saccharomyces cerevisiae, whereas exposure to mating-pheromone causes cells to differentiate into gametes. Although these two pathways respond to very different stimuli and generate very different responses, th...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-009-0274-6

    authors: Draper E,Dubrovskyi O,Bar EE,Stone DE

    更新日期:2009-12-01 00:00:00

  • Carbon source regulation of a dextranase gene from the filamentous fungus Penicillium minioluteum.

    abstract::The regulation of dextranase (dexA) gene expression in the filamentous fungus Penicillium minioluteum grown on different carbon sources was studied. Growth in the presence of dextran leads to high expression of the dextranase enzyme, but growth in starch, glucose, glycerol, lactose and sorbitol did not. Dextran induce...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940000119

    authors: García B,Rodríguez E

    更新日期:2000-06-01 00:00:00

  • Tmt1: the first LTR-retrotransposon from a Tuber spp.

    abstract::Retrotransposons are suitable targets for developing molecular markers for population genetics studies. Transposable elements have not yet been isolated from ectomycorrhizal fungi of the genus Tuber. In this paper, we report on the isolation and characterization of Tmt1, an LTR-retrotransposon from Tuber melanosporum....

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-007-0155-9

    authors: Riccioni C,Rubini A,Belfiori B,Passeri V,Paolocci F,Arcioni S

    更新日期:2008-01-01 00:00:00

  • Cloning and expression of Hormoconis resinae glucoamylase P cDNA in Saccharomyces cerevisiae.

    abstract::A cDNA coding for glucoamylase P of Hormoconis resinae was cloned using a synthetic oligonucleotide probe coding for a peptide fragment of the purified enzyme and polyclonal anti-glucoamylase antibodies. Nucleotide-sequence analysis revealed an open reading frame of 1848 base pairs coding for a protein of 616 amino-ac...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00324663

    authors: Vainio AE,Torkkeli HT,Tuusa T,Aho SA,Fagerström BR,Korhola MP

    更新日期:1993-07-01 00:00:00

  • Comparison of the lignin-degrading white rot fungi Phanerochaete chrysosporium and Sporotrichum pulverulentum at the DNA level.

    abstract::DNA-hybridisation studies showed a close relationship between Phanerochaete chrysosporium ME446, most used in lignin degradation studies, and Sporotrichum pulverulentum Novobranova, the other standard lignin degrading strain. Two other strains of P. chrysosporium were both less related. We show that P. chrysosporium M...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00410436

    authors: Raeder U,Broda P

    更新日期:1984-09-01 00:00:00

  • Characterization of the nuclear gene encoding chloroplast ribosomal protein S13 from Arabidopsis thaliana.

    abstract::We have characterised a cDNA clone and a nuclear gene encoding the chloroplast 30 s ribosomal protein S13 from Arabidopsis thaliana. The identification is based on the high similarity of the predicted amino-acid sequence with eubacterial S13 protein sequences, and immunodetection of a 14.5-kDa chloroplast ribosomal po...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00326432

    authors: Kumar R,Drouaud J,Raynal M,Small I

    更新日期:1995-09-01 00:00:00

  • Identification of genes that are preferentially expressed in conidiogenous cell development of Metarhizium anisopliae by suppression subtractive hybridization.

    abstract::The insect pathogenic fungus Metarhizium anisopliae is widely used as an insect biocontrol agent. The M. anisopliae conidium plays an important role in pathogenesis and disease transmission. The aim of this study was to identify genes whose expression is up-regulated during conidiogenous cell development. This is a po...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-009-0242-1

    authors: Peng G,Xie L,Hu J,Xia Y

    更新日期:2009-06-01 00:00:00

  • The isoprenoid pathway in the ectomycorrhizal fungus Tuber borchii Vittad.: cloning and characterisation of the tbhmgr, tbfpps and tbsqs genes.

    abstract::The isoprenoid pathway of the ectomycorrhizal fungus Tuber borchii Vittad is investigated to better understand the molecular mechanisms at work, in particular during the maturation of the complex ascomata (the so-called "truffles"). Three T. borchii genes coding for the most important regulatory enzymes of the isopren...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-006-0097-7

    authors: Guidi C,Zeppa S,Annibalini G,Pierleoni R,Guescini M,Buffalini M,Zambonelli A,Stocchi V

    更新日期:2006-12-01 00:00:00

  • L1-like non-LTR retrotransposons in the yeast Candida albicans.

    abstract::Non-LTR retrotransposons (also known as LINEs) have had a major influence on the structure of many eukaryote genomes. They are abundant in many multicellular eukaryotes, including mammals, but appear to be absent from the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. This absence has, to date, preclud...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940000181

    authors: Goodwin TJ,Ormandy JE,Poulter RT

    更新日期:2001-04-01 00:00:00

  • Functional analysis of the yeast genome: use of two-dimensional gel electrophoresis to detect genes in randomly cloned DNA sequences.

    abstract::Genes are overexpressed when present in yeast cells on multicopy plasmids. Taking advantage of the protein amplification which results from this overexpression, a method has been developed for large scale detection of yeast genes on randomly cloned DNA sequences. It is based on the analysis, by two-dimensional gel ele...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00318208

    authors: Thoraval D,Régnacq M,Neuville P,Boucherie H

    更新日期:1990-11-01 00:00:00

  • The use of RAPD markers in the genetic analysis of the plant pathogenic fungus Cladosporium fulvum.

    abstract::RAPD markers have been obtained in Cladosporium fulvum, in order to establish a genetic map based on mitotic recombination in this imperfect fungus. Segregation analysis has provided molecular evidence of a high degree of recombination during the parasexual cycle, and of the ploidy level of the parasexual progeny. A m...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00351783

    authors: Arnau J,Housego AP,Oliver RP

    更新日期:1994-05-01 00:00:00

  • A Saccharomyces paradox: chromosomes from different species are incompatible because of anti-recombination, not because of differences in number or arrangement.

    abstract::Many species are able to hybridize, but the sterility of these hybrids effectively prevents gene flow between the species, reproductively isolating them and allowing them to evolve independently. Yeast hybrids formed by Saccharomyces cerevisiae and Saccharomyces paradoxus parents are viable and able to grow by mitosis...

    journal_title:Current genetics

    pub_type: 杂志文章,评审

    doi:10.1007/s00294-019-01038-x

    authors: Ono J,Greig D

    更新日期:2020-06-01 00:00:00

  • L-ornithine transaminase synthesis in Saccharomyces cerevisiae: Induction by allophanate, intermediate and inducer of the urea degradative pathway adds to arginine induction.

    abstract::Yeast ornithine transaminase is known to be induced by arginine and ornithine, through the action of regulatory elements common to arginase induction. We show here that it is subject to a second induction circuit, that which is responsible for urea amidolyase and urea permease induction by allophanate and defined by t...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00376788

    authors: Hennaut C

    更新日期:1981-09-01 00:00:00

  • The endpoints of an inversion in wheat chloroplast DNA are associated with short repeated sequences containing homology to att-lambda.

    abstract::The endpoints of an inversion in wheat chloroplast DNA are shown to be associated with copies of a short repeated sequence. Recombination across the repeats in an inverted configuration may have been responsible for the inversion, although they are currently in a direct orientation owing to a second inversion. The rep...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00636479

    authors: Howe CJ

    更新日期:1985-01-01 00:00:00

  • Amphimeric mitochondrial genomes of petite mutants of yeast. II. A model for the amplification of amphimeric mitochondrial petite DNA.

    abstract::A model for the recombination-directed replication and amplification of the mtDNA of amphimeric petite mutants of S. cerevisiae is proposed. Replication of an amphimeric master basic unit datA would be initiated in the inverted components a and A. The initiation of replication should be associated with the amphimeric ...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940050112

    authors: Rayko E,Goursot R

    更新日期:1996-07-31 00:00:00

  • Temperature sensitivity of RNA editing and intron splicing reactions in the plastid ndhB transcript.

    abstract::Primary transcripts in higher plant cell organelles undergo a series of essential RNA processing steps, including 5'- and 3'-terminal maturation, intron splicing and RNA editing. Most of the protein factors involved in these RNA processing mechanisms are still unknown. Also, little is known about the environmental and...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-002-0278-y

    authors: Karcher D,Bock R

    更新日期:2002-04-01 00:00:00

  • A conserved role of the RSC chromatin remodeler in the establishment of nucleosome-depleted regions.

    abstract::The occupancy of nucleosomes governs access to the eukaryotic genomes and results from a combination of biophysical features and the effect of ATP-dependent remodelling complexes. Most promoter regions show a conserved pattern characterized by a nucleosome-depleted region (NDR) flanked by nucleosomal arrays. The conse...

    journal_title:Current genetics

    pub_type: 杂志文章,评审

    doi:10.1007/s00294-016-0642-y

    authors: Yague-Sanz C,Vázquez E,Sánchez M,Antequera F,Hermand D

    更新日期:2017-05-01 00:00:00

  • Biochemical and molecular characterization of a putative endoglucanase in Magnaporthe grisea.

    abstract::Microbial pathogens secrete an array of cell wall-degrading enzymes to break down the structure of the host cell wall to facilitate colonization of the host tissue. To better understand their role in the pathogenesis, a putative endoglucanase from Magnaporthe grisea was characterized in this paper. SignalP-3.0 analysi...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-008-0179-9

    authors: Zhou J,Zheng XZ,Lan L,Lin CZ,Wu YB,Lin XJ,Ebbole D,Lu GD,Wang ZH

    更新日期:2008-04-01 00:00:00

  • A novel nuclear gene, CBT1, essential for mitochondrial cytochrome b formation: terminal processing of mRNA and intron dependence.

    abstract::We describe a new nuclear gene, CBT1 (Cytochrome B Termination), specifically involved in the generation of mature mRNA of cytochrome b in yeast mitochondria. Disruption of CBT1 (corresponding to ORF YKL 208W) results in a respiratory deficiency (no growth on acetate and ethanol, a reduced growth on glycerol, and a mo...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940050262

    authors: Rieger KJ,Aljinovic G,Lazowska J,Pohl TM,Slonimski PP,Aljinovic G

    更新日期:1997-09-01 00:00:00

  • A small gene family in barley encodes ribosomal proteins homologous to yeast YL17 and L22 from archaebacteria, eubacteria, and chloroplasts.

    abstract::The amino acid sequences of two barley ribosomal proteins, termed HvL17-1 and HvL17-2, were decoded from green leaf cDNA clones. The N-terminal sequences of the derived barley proteins are 48% identical to the N-terminal amino acid sequence of protein YL17 from the large subunit of yeast cytoplasmic ribosomes. Via arc...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00309605

    authors: Madsen LH,Kreiberg JD,Gausing K

    更新日期:1991-05-01 00:00:00

  • The gene encoding Arabidopsis thaliana mitochondrial ribosomal protein S13 is a recent duplication of the gene encoding plastid S13.

    abstract::A gene encoding mitochondrial S13 is generally present in the mitochondrial genome of higher plants, but is lacking from the Arabidopsis thaliana mitochondrial genome. Previous research has failed to identify a nuclear gene capable of encoding a mitochondrial S13 protein or the protein itself. Doubts have even been ra...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-002-0271-5

    authors: Mollier P,Hoffmann B,Debast C,Small I

    更新日期:2002-03-01 00:00:00

  • RNA silencing in Chlamydomonas: mechanisms and tools.

    abstract::The generation of a comprehensive EST library and the sequencing of its genome set the stage for reverse genetics approaches in Chlamydomonas reinhardtii. However, these also require tools for the specific downregulation of target gene expression. Consequently, a large number of diverse constructs were developed aimed...

    journal_title:Current genetics

    pub_type: 杂志文章,评审

    doi:10.1007/s00294-005-0042-1

    authors: Schroda M

    更新日期:2006-02-01 00:00:00