Abstract:
:Retrotransposons are suitable targets for developing molecular markers for population genetics studies. Transposable elements have not yet been isolated from ectomycorrhizal fungi of the genus Tuber. In this paper, we report on the isolation and characterization of Tmt1, an LTR-retrotransposon from Tuber melanosporum. The Tmt1 sequence shows relatedness to Ty3/gypsy retrotransposons from which it differentiates for the presence of a dUTPase extra-domain between protease and reverse transcriptase. Phylogenetic analyses suggest a horizontal transfer of the dUTPase gene (dut) from a fungal host genome. The presence of non-identical LTRs and degenerate ORFs substantiate an ancient integration of Tmt1 in T. melanosporum genome. Furthermore, transcripts analyses proved an inactive status of Tmt1, whereas Southern analysis showed that Tmt1 is a repetitive T. melanosporum species-specific element. Tmt1-based markers will help us to gain more insights into population biology in this fungal species.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Riccioni C,Rubini A,Belfiori B,Passeri V,Paolocci F,Arcioni Sdoi
10.1007/s00294-007-0155-9subject
Has Abstractpub_date
2008-01-01 00:00:00pages
23-34issue
1eissn
0172-8083issn
1432-0983journal_volume
53pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::Uridine diphosphoglucose is not the sole donor for trehalose synthesis in yeast cells: an ADPG-dependent trehalose synthase, has been identified in mutant strains with undetectable UDPG-dependent trehalose-6-P synthase activity. Genetic and chromatographic studies indicate that the two activities correspond to differe...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00393399
更新日期:1989-08-01 00:00:00
abstract::The chloroplast genome of black pine (Pinus thunbergii), a gymnosperm, contains 32 different tRNA genes, 30 of which correspond to those previously identified in tobacco and rice chloroplast genomes. Two additional genes encode tRNA(Pro) (GGG) and tRNA(Arg) (CCG); the former is newly identified while the latter is pre...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00313804
更新日期:1994-08-01 00:00:00
abstract::Chemoreceptor-based signaling is a major bacterial signal transduction mechanism. Escherichia coli, the traditional model, has five chemoreceptors. Recent genome analyses have shown that many bacteria have a much higher number of chemoreceptors. Pseudomonas putida KT2440 is an alternative model that has 27 chemorecept...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-016-0646-7
更新日期:2017-06-01 00:00:00
abstract::Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNN...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351689
更新日期:1992-04-01 00:00:00
abstract::A cDNA coding for glucoamylase P of Hormoconis resinae was cloned using a synthetic oligonucleotide probe coding for a peptide fragment of the purified enzyme and polyclonal anti-glucoamylase antibodies. Nucleotide-sequence analysis revealed an open reading frame of 1848 base pairs coding for a protein of 616 amino-ac...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00324663
更新日期:1993-07-01 00:00:00
abstract::Microorganisms have the ability to adapt and respond to different environmental conditions, whether they are stressful or not. Although the detection and/or responding mechanisms are often unknown, a large number of proteins may participate in signal transduction pathways involved in environmental stimulus to induce p...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-017-0772-x
更新日期:2018-06-01 00:00:00
abstract::Current strategies to improve the secretion of heterologous proteins from Aspergillus niger include the manipulation of chaperones and foldases specific to the endoplasmic reticulum (ER). Here we report the isolation of a gene, pdiA, encoding a putative protein disulphide isomerase (PDI) from A. niger using the Saccha...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050187
更新日期:1997-02-01 00:00:00
abstract::The wheat mitochondrial (mt) cox3 has been localized and sequenced. The gene exists as a single copy in the wheat mt master chromosome and is transcribed into a single 1.2 kb RNA, whose extremities have been mapped. Comparison of the wheat and Oenothera cox3 sequences gives ambiguous indications concerning the amino a...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00313247
更新日期:1990-01-01 00:00:00
abstract::In this paper we report the cloning, sequencing and functional characterization of CEN12 and an associated autonomously replicating sequence (ARS) from the budding yeast Saccharomyces cerevisiae. In the course of studying a dynamin-related gene, DNM1, we previously physically mapped the gene to chromosome 12. Genetic ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00518162
更新日期:1995-11-01 00:00:00
abstract::A hybrid selectable marker for transformation was constructed by placing the promoter (TEF1p) from the gene encoding the Aureobasidium pullulans translation elongation factor 1-alpha (TEF1) adjacent to the 5' end of the Escherichia coli hygromycin B phosphotransferase gene (HPT). Plasmids containing this hybrid gene (...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00313195
更新日期:1995-12-01 00:00:00
abstract::The filamentous fungus Coleophoma empetri F-11899 produces an echinocandin-like compound FR901379, the original source for micafungin which is prescribed to treat deep-seated mycoses. Despite its industrial importance, no genetic information on C. empetri F-11899 is currently available. To characterize FR901379 biosyn...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-009-0275-5
更新日期:2009-12-01 00:00:00
abstract::We have constructed a gene coding for the 12-kDa intermediate form of the 2s methionine-rich protein from Bertholletia excelsa seeds. This protein, expressed intracellularly in yeast, is characterised by a 20-min half-life. By adding 11 amino acids corresponding to the peroxisome-targeting sequence (PTSc) of luciferas...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309924
更新日期:1994-11-01 00:00:00
abstract::The Cys6Zn2 DNA-binding domain transcription factor Pdr1 is a central regulator of drug resistance in the pathogenic yeast Candida glabrata. In this review, I discuss the multiple control mechanisms modulating the function of this positive transcriptional regulator. Available data suggest that Pdr1 activity is restrai...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-018-0870-4
更新日期:2019-02-01 00:00:00
abstract::The endpoints of an inversion in wheat chloroplast DNA are shown to be associated with copies of a short repeated sequence. Recombination across the repeats in an inverted configuration may have been responsible for the inversion, although they are currently in a direct orientation owing to a second inversion. The rep...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00636479
更新日期:1985-01-01 00:00:00
abstract::A vector was constructed for transformation of the plant pathogenic fungus Fusarium solani. The promoter 35Sp, from cauliflower mosaic virus, was fused to the bacterial gene APH(3')II, which confers resistance to the aminoglycoside antibiotic G418. Two transformation procedures were developed: one using isolated funga...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00376799
更新日期:1989-06-01 00:00:00
abstract::The previous ovservation that in dividing sister cells of Schizosaccharomyces pombe only one of two parallel divisions can be accompanied by a switch of mating type, herein termed "Miyata's rule", has been confirmed in pedigrees of diploid cells heterozygous for the mat2-Pm-B102 allele. Moreover, this rule appears to ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00417817
更新日期:1984-04-01 00:00:00
abstract::Laccases are phenol-oxidizing, multicopper enzymes produced by fungi, plants, insects and bacteria. Fungal laccases are involved in ecologically important processes such as decomposition of lignocellulose (wood and plant material). In this work, in order to find out the role of fungal laccases upon wood colonisation a...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0090-1
更新日期:2006-11-01 00:00:00
abstract::Genes are overexpressed when present in yeast cells on multicopy plasmids. Taking advantage of the protein amplification which results from this overexpression, a method has been developed for large scale detection of yeast genes on randomly cloned DNA sequences. It is based on the analysis, by two-dimensional gel ele...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00318208
更新日期:1990-11-01 00:00:00
abstract::By using the fluorochrome 4'-6-diamidino-2-phenylindole (DAPI) to stain DNA one can follow the pattern of events affecting plastid DNA which occur in the formation and maturation of individual zygotes of the green flagellate Chlamydomonas moewusii. This species, like C. reinhardi, expresses uniparental inheritance of ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00434892
更新日期:1983-06-01 00:00:00
abstract::A screening of haploid yeast strains for enhanced resistance to nitrogen mustard (HN2) yielded a recessive mutant allele, hnm1, that conferred hyper-resistance (HYR) to HN2. Diploids, homo- or heterozygous for the HNM1 locus, exhibit normal wild-type like resistance while homozygosity for hnm1 leads to the phenotype H...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00318378
更新日期:1990-10-01 00:00:00
abstract::Approximately, 1% of the genes in eukaryotic genomes encode for helicases, which make the number of helicases expressed in the cell considerably high. Helicases are motor proteins that participate in many central aspects of the nuclear and mitochondrial genomes, and based on their helicase motif conservation, they are...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0675-2
更新日期:2017-08-01 00:00:00
abstract::The mitochondrial (mt) genome of the sea anemone Metridium senile contains genes for only two transfer RNAs (tRNAs), tRNAf-Met and tRNATrp. Experiments were conducted to seek evidence for the occurrence of functional tRNAs corresponding to these genes and for the participation of nuclear DNA-encoded tRNAs in mt-protei...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-013-0395-9
更新日期:2013-08-01 00:00:00
abstract::Glutathione (GSH) is an abundantly produced low-molecular-weight (LMW) thiol in many organisms. However, a number of Gram-positive bacteria do not produce GSH, but instead produce bacillithiol (BSH) as one of the major LMW thiols. Similar to GSH, studies have found that BSH has various roles in the cell, including pro...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-015-0511-0
更新日期:2016-02-01 00:00:00
abstract::Trichoderma reesei was transformed to hygromycin B resistance using a novel vector, which contains the E. coli hygromycin B phosphotransferase gene (hph) fused between promoter and terminator elements of the homologous Trichoderma pki1 (coding for pyruvate kinase) and cbh2 (coding for cellobiohydrolase II) genes, resp...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351679
更新日期:1994-06-01 00:00:00
abstract::The Saccharomyces cerevisiae open reading frame YDL202w has been characterised in the course of the EUROFAN yeast genome analysis program. Disruption of YDL202w causes a respiratory deficient phenotype accompanied by a loss of mitochondrial DNA. This phenotype is usually found in mutants defective in mitochondrial rep...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050221
更新日期:1997-05-01 00:00:00
abstract::In fungi, sexual reproduction primarily depends on the interaction between peptide pheromones and their receptors. Most ascomycete fungi produce two classes of peptide mating pheromones, a simple peptide and a modified peptide. These peptides are recognized by their corresponding receptors on the surface of cells of t...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-019-00968-w
更新日期:2019-10-01 00:00:00
abstract::In Saccharomyces cerevisiae, the HAP transcriptional complex is involved in the fermentation-respiration shift. This complex is composed of four subunits. Three subunits are necessary for DNA-binding, whereas the Hap4p subunit, glucose-repressed, contains the transcriptional activation domain. Hap4p is the key regulat...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-004-0556-y
更新日期:2005-03-01 00:00:00
abstract::Seven MboI fragments spanning the mitochondrial apocytochrome b gene in Saccharomyces cerevisiae strain D273-10B were cloned in the BamHI site of the integrative yeast vector YIp5 and the capacity for autonomous replication was subsequently assayed in yeast. The positive correlation found between the ars-like activity...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00368060
更新日期:1987-01-01 00:00:00
abstract::The search for novel pathological and functional amyloids represents one of the most important tasks of contemporary biomedicine. Formation of pathological amyloid fibrils in the aging brain causes incurable neurodegenerative disorders such as Alzheimer's, Parkinson's Huntington's diseases. At the same time, a set of ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-017-0759-7
更新日期:2018-04-01 00:00:00
abstract::The ribosomal DNA repeat unit of Sphaeronaemella fimicola was found to be a 13.7-kb tandem repeat with a relatively long nontranscribed spacer (NTS) and an unusually compact ribosomal RNA gene cluster. The DNA sequence of an 850-bp PCR amplification product containing the 3' end of the small subunit rRNA (SSrRNA) gene...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00310899
更新日期:1993-01-01 00:00:00