L-ornithine transaminase synthesis in Saccharomyces cerevisiae: Induction by allophanate, intermediate and inducer of the urea degradative pathway adds to arginine induction.

abstract：:Retrotransposons are suitable targets for developing molecular markers for population genetics studies. Transposable elements have not yet been isolated from ectomycorrhizal fungi of the genus Tuber. In this paper, we report on the isolation and characterization of Tmt1, an LTR-retrotransposon from Tuber melanosporum....

journal_title：Current genetics

authors： Riccioni C,Rubini A,Belfiori B,Passeri V,Paolocci F,Arcioni S

更新日期：2008-01-01 00:00:00

abstract：:Approximately, 1% of the genes in eukaryotic genomes encode for helicases, which make the number of helicases expressed in the cell considerably high. Helicases are motor proteins that participate in many central aspects of the nuclear and mitochondrial genomes, and based on their helicase motif conservation, they are...

journal_title：Current genetics

authors： Sabouri N

更新日期：2017-08-01 00:00:00

abstract：:Cellulases belong to the large family of glycosyl hydrolases (GHs) and are produced by a variety of bacteria and fungi. These extracellular enzymes act as endoglucanases (EGs), cellobiohydrolases or beta-glucosidases. In this paper, we describe molecular screening for EGs from the GH family 12. Using three homologous ...

journal_title：Current genetics

authors： Goedegebuur F,Fowler T,Phillips J,van der Kley P,van Solingen P,Dankmeyer L,Power SD

更新日期：2002-05-01 00:00:00

abstract：:Sister chromatid cohesion is essential for chromosome segregation both in mitosis and meiosis. Cohesion between two chromatids is mediated by a protein complex called cohesin. The loading and unloading of the cohesin are tightly regulated during the cell cycle. In vertebrate cells, cohesin is released from chromosomes...

journal_title：Current genetics

authors： Challa K,Shinohara M,Shinohara A

更新日期：2019-08-01 00:00:00

abstract：:The cloning and characterization of the Thcut1 gene, which encodes a cutinase protein of the biocontrol fungus Trichoderma harzianum T34, is reported. Cutinases, which are secreted enzymes that hydrolyse cutin, belong to a class of serine esterases able to hydrolyze fatty acid esters and emulsified triglycerides. The ...

journal_title：Current genetics

authors： Rubio MB,Cardoza RE,Hermosa R,Gutiérrez S,Monte E

更新日期：2008-12-01 00:00:00

abstract：:Studies of motility in Physarum polycephalum have concentrated on the well-defined actomyosin system in plasmodia. It is clear from recent genetic studies in lower eukaryotes that myosin is involved in a number of physiological processes in addition to the contractile functions previously ascribed to the classical typ...

journal_title：Current genetics

authors： Murray M,Foxon J,Sweeney F,Orr E

更新日期：1994-02-01 00:00:00

abstract：:Adaptation by natural selection might improve the fitness of an organism and its probability to survive in unfavorable environmental conditions. Decoding the genetic basis of adaptive evolution is one of the great challenges to deal with. To this purpose, Saccharomyces cerevisiae has been largely investigated because ...

journal_title：Current genetics

authors： Tosato V,Sims J,West N,Colombin M,Bruschi CV

更新日期：2017-05-01 00:00:00

abstract：:Nucleic acid extracted from killer strains of Saccharomyces cerevisiae and run on agarose gels under sub-optimal conditions for M dsRNA yielded two species of L dsRNA (L1 and L2) which migrated at slightly different rates. L2 dsRNA was shown to be dependent upon MAK3, MAK10 and PES1 but not MAK1 nuclear genes for its ...

journal_title：Current genetics

authors： El-Sherbeini M,Bevan EA,Mitchell DJ

更新日期：1983-03-01 00:00:00

abstract：:To gain a deeper insight into the evolution of mating-type genes from filamentous ascomycetes, a comprehensive sequence analysis of PCR-amplified sequences corresponding to A- and a-specific mating-type sequences was undertaken. The study included nine homothallic (compatible) and eight heterothallic (incompatible) me...

journal_title：Current genetics

authors： Pöggeler S

更新日期：1999-10-01 00:00:00

abstract：:In Aspergillus nidulans, carbon catabolite repression (CCR) is mediated by the global repressor protein CreA. The deubiquitinating enzyme CreB is a component of the CCR network. Genetic interaction was confirmed using a strain containing complete loss-of-function alleles of both creA and creB. No direct physical inter...

journal_title：Current genetics

authors： Alam MA,Kelly JM

更新日期：2017-08-01 00:00:00

abstract：:Loss of heterozygosity (LOH) in a vegetatively growing diploid cell signals irregularity of mitosis. Therefore, assays of LOH serve to discover pathways critical for proper replication and segregation of chromosomes. We screened for enhanced LOH in a whole-genome collection of diploid yeast strains in which a single g...

journal_title：Current genetics

authors： Tutaj H,Pogoda E,Tomala K,Korona R

更新日期：2019-04-01 00:00:00

abstract：:Studies on replicative and chronological aging in Saccharomyces cerevisiae have greatly advanced our understanding of how longevity is regulated in all eukaryotes. Chronological lifespan (CLS) of yeast is defined as the age-dependent viability of non-dividing cell populations. A number of nutrient sensing and signal t...

journal_title：Current genetics

authors： Zhang N,Cao L

更新日期：2017-10-01 00:00:00

abstract：:DNA-damage tolerance (DDT) is employed by eukaryotes to deal with replication blocks on the template strand, and is divided into two parallel pathways that are activated by sequential ubiquitination of proliferating cell nuclear antigen (PCNA) at the Lys164 residue. Rad6-Rad18-mediated PCNA monoubiquitination promotes...

journal_title：Current genetics

authors： Li F,Ball LG,Fan L,Hanna M,Xiao W

更新日期：2018-04-01 00:00:00

abstract：:Transforming DNAs form cointegrates in Aspergillus nidulans by homologous and non-homologous recombination as well as by end-to-end ligation of linear fragments. This process has been studied by means of a model in which the linkage of a marker gene to the origin of autonomous replication AMA1 was selected for. Recomb...

journal_title：Current genetics

authors： Aleksenko AY

更新日期：1994-10-01 00:00:00

abstract：:A simple method is described for demonstrating adaptation to alkylation damage in Aspergillus nidulans. One wild type, two MNNG-sensitive, and one MNNG-resistant strain all showed improvement in colony growth when challenged with MNNG following appropriate inducing pretreatments. Other alkylating agents (MMS, EMS) cou...

journal_title：Current genetics

authors： Hooley P,Shawcross SG,Strike P

更新日期：1988-11-01 00:00:00

abstract：:The nucleotide sequence of the ERG12 gene, encoding mevalonate kinase, from Saccharomyces cerevisiae is presented. The longest open reading frame may code for a protein containing 443 amino acids with a deduced relative molecular mass of 48,500. The analysis of the nucleotide sequence reveals a complete identity with ...

journal_title：Current genetics

authors： Oulmouden A,Karst F

更新日期：1991-01-01 00:00:00

abstract：:By completing and correcting the sequence of a 1.8 kb DNA segment downstream of the oxi2 gene of Saccharomyces cerevisiae, a long, potentially coding sequence ("RF2") has been identified. The sequence is rather closely related to the RF1 open reading frame, downstream of the oxil gene, and, further, to the major famil...

journal_title：Current genetics

authors： Michel F

更新日期：1984-05-01 00:00:00

abstract：:A class of large site-specific deletions (del-B) occurs with exceptionally-high frequencies of 10(-3) in the mitochondrial COX1 gene of Mn(2+)-treated yeast cells. This work shows that del-B deletions are associated with COX1 intron aI1. All five deletion mutants studied have their upstream end at the authentic 3' spl...

journal_title：Current genetics

authors： Weiller GF

更新日期：1994-11-01 00:00:00

abstract：:Meiotic fine-structure maps of two efficient UGA suppressors of Schizosaccharomyces pombe which are known (sup8-e) or inferred (sup10-e) to code for two leucine tRNAs carrying the mutant anticodon U(*)CA (Kohli et al. 1979, 1980a, b; Wetzel et al. 1979; Mao et al. 1981) are presented. In both cases, the recombination ...

journal_title：Current genetics

authors： Munz P,Dorsch-Häsler K,Leupold U

更新日期：1983-04-01 00:00:00

abstract：:Mitochondrial DNA from etiolated seedling of male-fertile (F) and male-sterile (S) sunflower (Helianthus annuus L.) has been investigated for the first time in an isogenic couple HA89. 1. The mt-DNAs isolated from the S and F cytoplasms give a sharp and symmetrical band at 1.706 g/ml in an analytical CsCI gradient ult...

journal_title：Current genetics

authors： Leroy P,Bazetoux S,Quétier F,Delbut J,Bervillé A

更新日期：1985-03-01 00:00:00

abstract：:The yeast OMP decarboxylase gene has been cloned in the phage fd in both orientations to obtain pure DNA of each strand. With these probes the transcription of each strand of the yeast gene was measured in wild type yeast, either repressed or induced, in a yeast strain transformed by an hybrid plasmid containing the O...

journal_title：Current genetics

authors： Hubert JC,Bach ML,Lacroute F

更新日期：1980-10-01 00:00:00

abstract：:We describe the preparation of an in vitro translation system from heat shock-treated Aspergillus nidulans, capable of supporting efficient and faithful synthesis of proteins from natural and in vitro transcribed eukaryotic messages. In vitro synthesized prepro-alpha-factor was translocated across Aspergillus nidulans...

journal_title：Current genetics

authors： Devchand M,Gwynne D,Buxton FP,Davies RW

更新日期：1988-12-01 00:00:00

abstract：:La France disease of the cultivated mushroom, Agaricus bisporus, is known to be associated with the presence of a number of dsRNA segments. The nucleotide sequences of the dsRNAs M2 (1.3 kb), M1 (1.55 kb) and L3 (2.8 kb), invariably associated with the disease, were determined. Putative coding sequences for proteins w...

journal_title：Current genetics

authors： Harmsen MC,Tolner B,Kram A,Go SJ,de Haan A,Wessels JG

更新日期：1991-07-01 00:00:00

abstract：:Blasticidin S-resistant mutants of S. cerevisiae were isolated and characterized. Resistant mutations were found to fall into two complementation groups. A single recessive nuclear gene was responsible for each group, donated as bls1 and bls2, respectively. A gene bls1 was linked to an ilv3 gene located on the right a...

journal_title：Current genetics

authors： Ishiguro J,Miyazaki M

更新日期：1985-01-01 00:00:00

abstract：:Chloroplast DNA from the fern Osmunda einnamomea was isolated by a sucrose gradient procedure utilizing PEG to stabilize chloroplasts. Analysis with the restriction endonucleases PvuII, Sacl and BstEII indicates a chloroplast genome size of 144 kb. A physical map of the fragments produced by these three enzymes was co...

journal_title：Current genetics

authors： Palmer JD,Stein DB

更新日期：1982-08-01 00:00:00

abstract：:Forces generated by molecular motors and the cytoskeleton move the nucleus and genome during many cellular processes, including cell migration and division. How these forces impact the genome, and whether cells regulate cytoskeletal forces to preserve genome integrity is unclear. We recently demonstrated that, in budd...

journal_title：Current genetics

authors： Estrem C,Moore JK

更新日期：2020-04-01 00:00:00

abstract：:Cyclosporin A is a potent and clinically-important immunosuppressive drug (SandimmunR). It is produced by the fungus Tolypocladium niveum. A transformation system for T. niveum ATCC34921 based on hygromycin selection was established. In order to obtain a T. niveum promoter, the cyclophilin gene was isolated using the ...

journal_title：Current genetics

authors： Weber G,Leitner E

更新日期：1994-11-01 00:00:00

abstract：:A phylogenetic investigation of the autosporic chlorophycean alga species Selenastrum capricornutum using the small subunit (SSU) rRNA gene revealed the unusual presence of six group IC1 introns. Previous studies showed that numerous green algal taxa contain group IC1 introns. However, whereas some algal species harbo...

journal_title：Current genetics

authors： Booton GC,Floyd GL,Fuerst PA

更新日期：2004-10-01 00:00:00

abstract：:In order to facilitate the process of epitope-tagging of yeast proteins, we have constructed two Saccharomyces cerevisiae-Escherichia coli shuttle vectors that allow fusion of a sequence encoding an epitope of the human c-myc protein at the 3' end of any gene. An example of the use of this technique is presented. ...

journal_title：Current genetics

authors： Reisdorf P,Maarse AC,Daignan-Fornier B

更新日期：1993-02-01 00:00:00

abstract：:The cif1 mutation of Saccharomyces cerevisiae causes inability to grow on glucose and related fermentable carbon sources. We have isolated two different suppressor mutations that allow growth on glucose of yeasts carrying the cif1 mutation. One of them, sci1-1, is recessive and caused inability to grow on non-fermenta...

journal_title：Current genetics

authors： Blázquez MA,Gancedo C

更新日期：1994-02-01 00:00:00