Abstract：
:Forces generated by molecular motors and the cytoskeleton move the nucleus and genome during many cellular processes, including cell migration and division. How these forces impact the genome, and whether cells regulate cytoskeletal forces to preserve genome integrity is unclear. We recently demonstrated that, in budding yeast, mutants that stabilize the microtubule cytoskeleton cause excessive movement of the mitotic spindle and nucleus. We found that increased nuclear movement results in DNA damage and increased time to repair the damage through homology-directed repair. Our results indicate that nuclear movement impairs DNA repair through increased tension on chromosomes and nuclear deformation. However, the previous studies have shown genome mobility, driven by cytoskeleton-based forces, aids in homology-directed DNA repair. This sets up an apparent paradox, where genome mobility may prevent or promote DNA repair. Hence, this review explores how the genome is affected by nuclear movement and how genome mobility could aid or hinder homology-directed repair.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Estrem C,Moore JKdoi
10.1007/s00294-019-01033-2subject
Has Abstractpub_date
2020-04-01 00:00:00pages
303-311issue
2eissn
0172-8083issn
1432-0983pii
10.1007/s00294-019-01033-2journal_volume
66pub_type
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