A mobile group II intron of a naturally occurring rearranged mitochondrial genome in Kluyveromyces lactis.


:Mitochondrial intron content is variable in the yeast Kluyveromyces lactis. Strains can be divided into three classes depending on the structure of the cytochrome oxidase subunit 1 (COX1) gene: (1) those containing intron K1 cox1.1, (2) those containing K1 cox1.2, 3 and 4 and, (3) those that contain all four introns. In addition, strains belonging to the first class (designated Type B strains), have an altered mitochondrial gene order relative to strains from classes (2) and (3) (Type A, Hardy et al. 1989). Crossing experiments reveal that K1 cox1.1 (a group II intron) transfers at high frequency (89%) to mitochondrial genomes lacking this intron. By contrast, the mobility of the remaining introns (all group I) is of the order of 7%.


Curr Genet


Current genetics


Skelly PJ,Hardy CM,Clark-Walker GD




Has Abstract


1991-07-01 00:00:00












  • Molecular and functional characterization of a mutant allele of the mitogen-activated protein-kinase gene SLT2(MPK1) rescued from yeast autolytic mutants.

    abstract::We have further characterized the functionality of the Saccharomyces cerevisiae gene SLT2(MPK1), coding for a MAP-kinase homolog essential for cell integrity, which is involved in the Pkc1p signalling pathway. This gene was isolated on the basis of its capacity to complement the thermosensitive-autolytic, osmotic-reme...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Martín H,Castellanos MC,Cenamor R,Sánchez M,Molina M,Nombela C

    更新日期:1996-05-01 00:00:00

  • A conserved role of the RSC chromatin remodeler in the establishment of nucleosome-depleted regions.

    abstract::The occupancy of nucleosomes governs access to the eukaryotic genomes and results from a combination of biophysical features and the effect of ATP-dependent remodelling complexes. Most promoter regions show a conserved pattern characterized by a nucleosome-depleted region (NDR) flanked by nucleosomal arrays. The conse...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Yague-Sanz C,Vázquez E,Sánchez M,Antequera F,Hermand D

    更新日期:2017-05-01 00:00:00

  • The KP4 killer protein gene family.

    abstract::Killer protein 4 (KP4) is a well studied viral toxin secreted by the maize smut fungus Ustilago maydis that kills sensitive Ustilago strains as well as inhibits Fusarium and plant root growth by inhibiting calcium uptake. Numerous small, cysteine-rich proteins have been shown to play a critical role in fungal-plant-ba...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Brown DW

    更新日期:2011-02-01 00:00:00

  • Debaryomyces hansenii, a highly osmo-tolerant and halo-tolerant yeast, maintains activated Dhog1p in the cytoplasm during its growth under severe osmotic stress.

    abstract::The HOG pathway is an important mitogen-activated protein kinase (MAPK) signal transduction pathway in Saccharomyces cerevisiae that mediates adaptation of cells to hyper-osmotic stress. Activation of this pathway causes rapid but transient, phosphorylation of the MAPK Hog1p. Phosphorylated Hog1p is rapidly transporte...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Sharma P,Meena N,Aggarwal M,Mondal AK

    更新日期:2005-09-01 00:00:00

  • Unexpectedly long 14S ribosomal RNA gene in Tetrahymena mitochondria.

    abstract::Extraction of RNA from Tetrahymena mitochondrial ribosomes yields several RNA species, including a "large" 21S molecule, a "small" 14S molecule, a 7S molecule, and other smaller RNAs. The molecular weight of the 14S rRNA indicates that it is about 1,300 bases in length. We have sequenced the 14S rRNA gene and, by alig...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Labriola J,Weiss I,Zapatero J,Suyama Y

    更新日期:1987-01-01 00:00:00

  • Deletions in the cob gene of yeast mtDNA and their phenotypic effect.

    abstract::Two cob- deletion mutants are characterized. One of them, M9410, is deleted for 911 bp of the noncoding sequences only which separate tRNAGlu and cob exon 1; it thus lacks most of the sequence encoding the 957 bp long cob leader (Bonitz et al. 1982) and some 20 bp 5' to it. The end points of this deletion coincide wit...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Dobres M,Gerbl-Rieger S,Schmelzer C,Mueller MW,Schweyen RJ

    更新日期:1985-01-01 00:00:00

  • L1-like non-LTR retrotransposons in the yeast Candida albicans.

    abstract::Non-LTR retrotransposons (also known as LINEs) have had a major influence on the structure of many eukaryote genomes. They are abundant in many multicellular eukaryotes, including mammals, but appear to be absent from the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. This absence has, to date, preclud...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Goodwin TJ,Ormandy JE,Poulter RT

    更新日期:2001-04-01 00:00:00

  • Cloning and characterization of a Schizophyllum gene with A beta 6 mating-type activity.

    abstract::The A-pathway of development in the basidiomycete fungus Schizophyllum commune may be activated by either of two mating-type loci, A alpha and A beta. A alpha consists of two multiallelic genes, Y and Z. Y contains a putative homeodomain; Z contains a homeodomain-related region. Non-self combinations of Y and Z form h...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Shen GP,Park DC,Ullrich RC,Novotny CP

    更新日期:1996-01-01 00:00:00

  • Pentose-phosphate pathway in Saccharomyces cerevisiae: analysis of deletion mutants for transketolase, transaldolase, and glucose 6-phosphate dehydrogenase.

    abstract::Deletion mutants for the yeast transketolase gene TKL1 were constructed by gene replacement. Transketolase activity was below the level of detection in mutant crude extracts. Transketolase protein could be detected as a single protein band of the expected size by Western-blot analysis in wild-type strains but not in t...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Schaaff-Gerstenschläger I,Zimmermann FK

    更新日期:1993-11-01 00:00:00

  • Starvation signals in yeast are integrated to coordinate metabolic reprogramming and stress response to ensure longevity.

    abstract::Studies on replicative and chronological aging in Saccharomyces cerevisiae have greatly advanced our understanding of how longevity is regulated in all eukaryotes. Chronological lifespan (CLS) of yeast is defined as the age-dependent viability of non-dividing cell populations. A number of nutrient sensing and signal t...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Zhang N,Cao L

    更新日期:2017-10-01 00:00:00

  • Variation of crossover association frequencies with various aberrant segregation classes in Ascobolus.

    abstract::1710 asci exhibiting mendelian or aberrant segregation at the b8 spore colour locus in Ascobolus were analysed from crosses between b8 mutants and the wild type in the presence of linked flanking markers. The frequencies of associated crossing-over were found to vary with the various classes of aberrant segregation an...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Nicolas A

    更新日期:1982-11-01 00:00:00

  • Transformation of the oomycete pathogen Phytophthora megasperma f. sp. glycinea occurs by DNA integration into single or multiple chromosomes.

    abstract::A procedure for stable transformation was developed for Phytophthora megasperma f. sp. glycinea, an oomycete pathogen of soybean. Transformants were obtained using a bacterial hygromycin resistance gene fused to a promoter and terminator from the ham34 gene of another oomycete, Bremia lactucae. Vector DNA, alone or co...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Judelson HS,Coffey MD,Arredondo FR,Tyler BM

    更新日期:1993-03-01 00:00:00

  • The intergenic region between the Vicia faba chloroplast tRNA(CAALeu) and tRNA(UAALeu) genes contains a partial copy of the split tRNA(UAALeu) gene.

    abstract::A cluster of three tRNA genes located on fragment Bam6a from Vicia faba chloroplast DNA has been sequenced: it contains the genes for tRNA(CAALeu), tRNA(UAALeu) and tRNA(Phe). The two tRNA(Leu) genes are separated by 443 bp and are transcribed divergently from different DNA strands. The intergenic region contains a se...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Bonnard G,Weil JH,Steinmetz A

    更新日期:1985-01-01 00:00:00

  • Isolation of a dsRNA virus from Dipodascus (Endomyces) magnusii.

    abstract::Virus-like particles (VLPs) of 40 nm diameter were isolated from the yeast-like fungus Dipodascus magnusii. These VLPs copurify with several linear double-stranded RNA molecules of different size. We have found some polymorphism in both the length and the number of these dsRNAs among six D. magnusii strains. Analysis ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Nosek J,Filipp D,Bederkova K,Griac P

    更新日期:1993-03-01 00:00:00

  • The CreB deubiquitinating enzyme does not directly target the CreA repressor protein in Aspergillus nidulans.

    abstract::Ubiquitination/deubiquitination pathways are now recognized as key components of gene regulatory mechanisms in eukaryotes. The major transcriptional repressor for carbon catabolite repression in Aspergillus nidulans is CreA, and mutational analysis led to the suggestion that a regulatory ubiquitination/deubiquitinatio...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Alam MA,Kamlangdee N,Kelly JM

    更新日期:2016-09-02 00:00:00

  • Multifaceted activities of DNA polymerase η: beyond translesion DNA synthesis.

    abstract::DNA polymerases are evolved to extend the 3'-OH of a growing primer annealed to a template DNA substrate. Since replicative DNA polymerases have a limited role while replicating structurally distorted template, translesion DNA polymerases mostly from Y-family come to the rescue of stalled replication fork and maintain...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Acharya N,Manohar K,Peroumal D,Khandagale P,Patel SK,Sahu SR,Kumari P

    更新日期:2019-06-01 00:00:00

  • Expression of Tri15 in Fusarium sporotrichioides.

    abstract::In the fungus Fusarium sporotrichioides, biosynthesis of trichothecene mycotoxins requires at least three genetic loci: a core 12-gene cluster, a smaller two-gene cluster, and a single-gene locus. Here, we describe the Tri15 gene, which represents a fourth locus involved in trichothecene biosynthesis. Tri15 is predict...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Alexander NJ,McCormick SP,Larson TM,Jurgenson JE

    更新日期:2004-03-01 00:00:00

  • Fungal effectors, the double edge sword of phytopathogens.

    abstract::Phyto-pathogenic fungi can cause huge damage to crop production. During millions of years of coexistence, fungi have evolved diverse life-style to obtain nutrients from the host and to colonize upon them. They deploy various proteinaceous as well as non-proteinaceous secreted molecules commonly referred as effectors t...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Pradhan A,Ghosh S,Sahoo D,Jha G

    更新日期:2020-11-04 00:00:00

  • Meiotic prophase-like pathway for cleavage-independent removal of cohesin for chromosome morphogenesis.

    abstract::Sister chromatid cohesion is essential for chromosome segregation both in mitosis and meiosis. Cohesion between two chromatids is mediated by a protein complex called cohesin. The loading and unloading of the cohesin are tightly regulated during the cell cycle. In vertebrate cells, cohesin is released from chromosomes...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Challa K,Shinohara M,Shinohara A

    更新日期:2019-08-01 00:00:00

  • Cointegration of transforming DNAs in Aspergillus nidulans: a model using autonomously-replicating plasmids.

    abstract::Transforming DNAs form cointegrates in Aspergillus nidulans by homologous and non-homologous recombination as well as by end-to-end ligation of linear fragments. This process has been studied by means of a model in which the linkage of a marker gene to the origin of autonomous replication AMA1 was selected for. Recomb...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Aleksenko AY

    更新日期:1994-10-01 00:00:00

  • Regulation of pho1-encoded acid phosphatase of Schizosaccharomyces pombe by adenine and phosphate.

    abstract::Expression of pho1-encoded acid phosphatase of Schizosaccharomyces pombe has been reported to be regulated by phosphate. In this communication we show that it is also regulated by adenine. Starving adenine auxotrophic strains for adenine leads to a drastic increase of the enzymatic activity while adenine represses thi...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Schweingruber ME,Edenharter E,Zurlinden A,Stockmaier KM

    更新日期:1992-10-01 00:00:00

  • Multiple drug resistance in the fission yeast Schizosaccharomyces pombe: Correlation between drug and amino acid uptake and membrane ATPase activities.

    abstract::Cyh3 and cyh4, multiple drug resistant strains of Schizosaccharomyces pombe, show a much reduced uptake of trichodermin, chloramphenicol, cycloheximide, L-lysine, glycine, L-threonine, L-glutamine, L-arginine and L-glutamic acid when compared to wild type. The plasma membrane and mitochondrial ATPase activities of the...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Johnston PA,Coddington A

    更新日期:1983-07-01 00:00:00

  • Cloning and expression of the OMP decarboxylase gene URA4 from Schizosaccharomyces pombe.

    abstract::URA4, the gene coding for orotidine monophosphate decarboxylase (OMPdecase), has been cloned from the fission yeast by homologous complementation and restricted in an Escherichia coli-Schizosaccharomyces pombe (E. coli-S. pombe) replicative plasmid to a 1.76 kb HindIII fragment. This plasmid is maintained at a high co...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Bach ML

    更新日期:1987-01-01 00:00:00

  • Partial sequence of the mitochondrial genome of the crustacean Daphnia pulex.

    abstract::A 4062-nucleotide (nt) fragment of the mitochondrial genome of the crustacean Daphnia pulex was sequenced and found to contain the complete genes for eight tRNAs and five proteins (ATP6, ATP8, COII, COIII, ND3) and the partial sequence of COI. In combination with data described previously, approximately 50% of the D. ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Crease TJ,Little TJ

    更新日期:1997-01-01 00:00:00

  • Disruption of the cyclosporin synthetase gene of Tolypocladium niveum.

    abstract::Cyclosporin A is a potent and clinically-important immunosuppressive drug (SandimmunR). It is produced by the fungus Tolypocladium niveum. A transformation system for T. niveum ATCC34921 based on hygromycin selection was established. In order to obtain a T. niveum promoter, the cyclophilin gene was isolated using the ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Weber G,Leitner E

    更新日期:1994-11-01 00:00:00

  • RNA silencing in Chlamydomonas: mechanisms and tools.

    abstract::The generation of a comprehensive EST library and the sequencing of its genome set the stage for reverse genetics approaches in Chlamydomonas reinhardtii. However, these also require tools for the specific downregulation of target gene expression. Consequently, a large number of diverse constructs were developed aimed...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Schroda M

    更新日期:2006-02-01 00:00:00

  • Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani.

    abstract::Sequence analysis of the rDNA region containing the internal transcribed spacer (ITS) regions and the 5.8s rDNA coding sequence was used to evaluate the genetic diversity of 45 isolates within and between anastomosis groups (AGs) in Rhizoctonia solani. The 5.8s rDNA sequence was completely conserved across all the AGs...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Kuninaga S,Natsuaki T,Takeuchi T,Yokosawa R

    更新日期:1997-09-01 00:00:00

  • Genome-wide expression analysis of a Saccharomyces cerevisiae strain deleted for the Tup1p-interacting protein Cdc73p.

    abstract::Tuplp is a general corepressor in Saccharomyces cerevisiae. We performed a split-ubiquitin screen with Tup1p as bait, in order to understand how Tuplp mediates repression. Cdc73p was the only component of a holoenzyme of transcription isolated as a Tuplp-interacting protein in this screen. Expression analysis of a str...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Kerkmann K,Lehming N

    更新日期:2001-07-01 00:00:00

  • A novel nuclear gene, CBT1, essential for mitochondrial cytochrome b formation: terminal processing of mRNA and intron dependence.

    abstract::We describe a new nuclear gene, CBT1 (Cytochrome B Termination), specifically involved in the generation of mature mRNA of cytochrome b in yeast mitochondria. Disruption of CBT1 (corresponding to ORF YKL 208W) results in a respiratory deficiency (no growth on acetate and ethanol, a reduced growth on glycerol, and a mo...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Rieger KJ,Aljinovic G,Lazowska J,Pohl TM,Slonimski PP,Aljinovic G

    更新日期:1997-09-01 00:00:00

  • Enhanced canavanine uptake is associated with nucleotide permeability in a thymidylate auxotroph of Saccharomyces cerevisiae.

    abstract::The recovery of spontaneous canavanine-resistant mutants is reduced dramatically in a strain of Saccharomyces cerevisiae that carries a suppressed can 1-100 allele and is permeable to and auxotrophic for thymidylate. This effect does not occur in an isogenic strain that neither takes up nor requires the nucleotide. Ho...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Kohalmi SE,Kunz BA

    更新日期:1989-02-01 00:00:00