Abstract:
:Caffeine is a well known base analogue and is cytotoxic to both animal and yeast cells. There are two possible mechanisms by which yeast cells tolerate caffeine concentrations higher than normal, by mutation or by physiological adaptation. We have isolated novel caffeine-resistant mutants of S. pombe which define three distinct genes caf2, caf3 and caf4. These mutants achieved a level of caffeine resistance which is presumed to represent the upper limit attainable by mutation. The caf2-caf4 mutations, as well as the previously identified caf1 mutation, confer UV-sensitivity, caffeine-resistant UV repair, impaired fertility and sporulation, as well as a lengthened cell cycle. They are partially dominant for caffeine resistance and recessive for UV sensitivity. Some auxotrophic caf3-89 double mutants show drastically decreased caffeine resistance. The caf4 mutant is more resistant to gamma-radiation than wild-type cells and shows pH-sensitive growth. As each caf mutation can, individually, confer maximum caffeine resistance to the cells, all four genes are expected to operate in the same pathway. This pathway might also be responsible for the physiological adaptation since adaptation is lost in caf1-caf4 mutants.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Benko Z,Miklos I,Carr AM,Sipiczki Mdoi
10.1007/s002940050233subject
Has Abstractpub_date
1997-06-01 00:00:00pages
481-7issue
6eissn
0172-8083issn
1432-0983journal_volume
31pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::Primary transcripts in higher plant cell organelles undergo a series of essential RNA processing steps, including 5'- and 3'-terminal maturation, intron splicing and RNA editing. Most of the protein factors involved in these RNA processing mechanisms are still unknown. Also, little is known about the environmental and...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-002-0278-y
更新日期:2002-04-01 00:00:00
abstract::Meiotic fine-structure maps of two efficient UGA suppressors of Schizosaccharomyces pombe which are known (sup8-e) or inferred (sup10-e) to code for two leucine tRNAs carrying the mutant anticodon U(*)CA (Kohli et al. 1979, 1980a, b; Wetzel et al. 1979; Mao et al. 1981) are presented. In both cases, the recombination ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00365633
更新日期:1983-04-01 00:00:00
abstract::Two plasmids containing Saccharomyces cerevisiae 2 µm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 µm DNA; in the presence of the LEU2 plasmids, 2 µm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 µm DNA loss has occurred, so that st...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00435686
更新日期:1980-12-01 00:00:00
abstract::DNA-hybridisation studies showed a close relationship between Phanerochaete chrysosporium ME446, most used in lignin degradation studies, and Sporotrichum pulverulentum Novobranova, the other standard lignin degrading strain. Two other strains of P. chrysosporium were both less related. We show that P. chrysosporium M...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00410436
更新日期:1984-09-01 00:00:00
abstract::Mitotic homologous recombination (HR) is an important mechanism for the repair of double-strand breakS and errors occurring during DNA replication. It is likely that the recombinational repair of DNA lesions occurs preferentially by sister chromatid exchanges that have no genetic consequences. However, most geneticall...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-002-0346-3
更新日期:2003-01-01 00:00:00
abstract::We have isolated and characterized a Ty element from a yeast cosmid library which exhibits several unusual features: it is flanked by non-homologous delta elements and directly associated with a singular delta element. A tRNA(Glu3) gene and tRNA(Cys) gene are found in conjunction with this element, located in opposite...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00420606
更新日期:1986-01-01 00:00:00
abstract::The CYP1 gene has previously been identified as coding for a positive trans active factor that activates the expression of CYC1 and CYP3, which are the structural genes for isol1- and iso2-cytochrome c. Two phenotypically distinct classes of CYP1 mutations can be obtained indicating that CYC1 and CYP3 are differential...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00418404
更新日期:1986-01-01 00:00:00
abstract::Although 29 tRNA genes have been deduced from the complete nucleotide sequence of the mitochondrial genome from the liverwort Marchantia polymorpha, a tRNAIle gene decoding AUU and AUC codons is conspicuously absent. In order to address the question of the possible involvement of nuclear-encoded tRNA, we isolated and ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050118
更新日期:1996-07-31 00:00:00
abstract::Six ultra violet (UV) mutageneses were performed on the spo76 UV-sensitive mutant of Sordaria macrospora. Spo76 shows an early centromere cleavage associated with an arrest at the first meiotic division and therefore does not form ascospores. Moreover, it exhibits altered pairing structure (synaptonemal complex), reve...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00447389
更新日期:1986-01-01 00:00:00
abstract::Thirteen chromosomal-sized DNA bands of the cultivated mushroom Agaricus bisporus have been resolved using the method of clamped homogeneous electric field (CHEF) electrophoresis. Using chromosome size standards from Schizosaccharomyces pombe, Saccharomyces cerevisiae and Candida albicans, the estimated size of the ch...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351712
更新日期:1993-12-01 00:00:00
abstract::The complete nucleotide sequence of the Morus mongolica chloroplast (cp) genome was reported and characterized in this study. The cp genome is a circular molecule of 158,459 bp containing a pair of 25,678 bp IR regions, separated by small and large single-copy regions of 19,736 and 87,363 bp, respectively. The number ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-015-0507-9
更新日期:2016-02-01 00:00:00
abstract::Mitochondrial genes coding for some components of the protein synthetic apparatus in S. douglasii have been studies in detail. A region containing stretches of high homology to the S. cerevisiae tRNA synthesis locus (TSL) and the tRNA(fmet) gene has been identified and sequenced. The organization of this region was ve...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00336483
更新日期:1991-03-01 00:00:00
abstract::The Cdc42p GTPase regulates multiple signal transduction pathways through its interactions with downstream effectors. Specific functional domains within Cdc42p are required for guanine-nucleotide binding, interactions with downstream effectors, and membrane localization. However, little is known about how Cdc42p is cl...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-004-0505-9
更新日期:2004-06-01 00:00:00
abstract::Spindle pole body (SPB; the microtubule organizing center in yeast) duplication is essential to form a bipolar spindle. The duplicated SPBs must then separate and migrate to opposite sides of the nucleus. We identified a novel functional relationship in SPB separation between the microtubule stabilizing protein Bik1p/...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0098-6
更新日期:2006-12-01 00:00:00
abstract::In the wood destroying fungus Schizophyllum commune, a well known subject for genetic studies, fruit bodies are produced not only in the course of the sexual cycle but also asexually. Sexual fruiting requires the establishment of a dikaryon which is under the control of the incompatibility factors A and B. Asexual fru...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00413309
更新日期:1979-12-01 00:00:00
abstract::In eukaryotes, genomic DNA is packaged into the nucleus together with histone proteins, forming chromatin. The fundamental repeating unit of chromatin is the nucleosome, a naturally symmetric structure that wraps DNA and is the substrate for numerous regulatory post-translational modifications. However, the biological...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-018-0910-0
更新日期:2019-04-01 00:00:00
abstract::The insect pathogenic fungus Metarhizium anisopliae is widely used as an insect biocontrol agent. The M. anisopliae conidium plays an important role in pathogenesis and disease transmission. The aim of this study was to identify genes whose expression is up-regulated during conidiogenous cell development. This is a po...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-009-0242-1
更新日期:2009-06-01 00:00:00
abstract::The amino acid sequences of two barley ribosomal proteins, termed HvL17-1 and HvL17-2, were decoded from green leaf cDNA clones. The N-terminal sequences of the derived barley proteins are 48% identical to the N-terminal amino acid sequence of protein YL17 from the large subunit of yeast cytoplasmic ribosomes. Via arc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309605
更新日期:1991-05-01 00:00:00
abstract::An exceptional mode of mtDNA inheritance involving separate maternal and paternal transmission routes has been reported recently in the mussel Mytilus edulis. This mode of inheritance provides an explanation for the high levels of heteroplasmy for two highly diverged genomes observed in males of this species. Here we ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02221509
更新日期:1996-04-01 00:00:00
abstract::In the photosynthetic chloroplast mutant PRB2A of Chlamydomonas reinhardtii the psbD mRNA is unstable. Three strains were isolated, in which the underlying site-directed mutation within the psbD 5' UTR (untranslated region) is suppressed. In all three suppressors, psbD RNA levels and RNA 5' maturation are restored to ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050020
更新日期:2000-02-01 00:00:00
abstract::Trichoderma reesei was transformed to hygromycin B resistance using a novel vector, which contains the E. coli hygromycin B phosphotransferase gene (hph) fused between promoter and terminator elements of the homologous Trichoderma pki1 (coding for pyruvate kinase) and cbh2 (coding for cellobiohydrolase II) genes, resp...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351679
更新日期:1994-06-01 00:00:00
abstract::The nucleotide sequences of the plastidal psbD genes of Bumilleriopsis filiformis and Porphyridium purpureum (encoding the D2 protein of photosystem II) are reported in this paper. The Bumilleriopsis sequence clusters together with Porphyridium when a most parsimonious protein tree of D2 sequences is constructed. A co...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351854
更新日期:1993-11-01 00:00:00
abstract::The mammalian HBP2 gene has been isolated by functional complementation of cells unable to undergo DNA replication in fission yeast. HBP2 is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As in other members of the family, the HMG box in...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940100241
更新日期:2001-09-01 00:00:00
abstract::Cellular reverse transcriptase-related (rvt) genes represent a novel class of reverse transcriptases (RTs), which are only distantly related to RTs of retrotransposons and retroviruses, but, similarly to telomerase RTs, are immobilized in the genome as single-copy genes. They have been preserved by natural selection t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-018-0844-6
更新日期:2018-12-01 00:00:00
abstract::Genes are overexpressed when present in yeast cells on multicopy plasmids. Taking advantage of the protein amplification which results from this overexpression, a method has been developed for large scale detection of yeast genes on randomly cloned DNA sequences. It is based on the analysis, by two-dimensional gel ele...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00318208
更新日期:1990-11-01 00:00:00
abstract::We have constructed a gene coding for the 12-kDa intermediate form of the 2s methionine-rich protein from Bertholletia excelsa seeds. This protein, expressed intracellularly in yeast, is characterised by a 20-min half-life. By adding 11 amino acids corresponding to the peroxisome-targeting sequence (PTSc) of luciferas...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309924
更新日期:1994-11-01 00:00:00
abstract::Mitochondrial DNA from etiolated seedling of male-fertile (F) and male-sterile (S) sunflower (Helianthus annuus L.) has been investigated for the first time in an isogenic couple HA89. 1. The mt-DNAs isolated from the S and F cytoplasms give a sharp and symmetrical band at 1.706 g/ml in an analytical CsCI gradient ult...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00420319
更新日期:1985-03-01 00:00:00
abstract::The S. cerevisiae strain h56 is a temperature-sensitive mit- mutant containing a single nucleotide substitution in the region 5' to the reading frame of the mitochondrial var1 gene. The mutation decreases the efficiency of processing of a precursor RNA such that little var1 mRNA is produced at the restrictive temperat...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00357168
更新日期:1994-03-01 00:00:00
abstract::Phyto-pathogenic fungi can cause huge damage to crop production. During millions of years of coexistence, fungi have evolved diverse life-style to obtain nutrients from the host and to colonize upon them. They deploy various proteinaceous as well as non-proteinaceous secreted molecules commonly referred as effectors t...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-020-01118-3
更新日期:2020-11-04 00:00:00
abstract::The atpB and atpE genes encode beta and epsilon subunits, respectively, of chloroplast ATP synthase and are co-transcribed in the plant species so far studied. In tobacco, an atpB gene-specific probe hybridizes to 2.7- and 2.3-kb transcripts. In addition to these, a probe from the atpE coding region hybridizes also to...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309558
更新日期:1994-09-01 00:00:00