Abstract:
:Mono-ubiquitinated histone H2B (H2B-Ub) is important for chromatin regulation of transcription, chromatin assembly, and also influences heterochromatin. In this review, we discuss the effects of H2B-Ub from nucleosome to higher-order chromatin structure. We then assess what is currently known of the role of H2B-Ub in heterochromatic silencing in budding and fission yeasts (S. cerevisiae and S. pombe), which have distinct silencing mechanisms. In budding yeast, the SIR complex initiates heterochromatin assembly with the aid of a H2B-Ub deubiquitinase, Ubp10. In fission yeast, the RNAi-dependent pathway initiates heterochromatin in the context of low H2B-Ub. We examine how the different silencing machineries overcome the challenge of H2B-Ub chromatin and highlight the importance of using these microorganisms to further our understanding of H2B-Ub in heterochromatic silencing pathways.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Zukowski A,Johnson AMdoi
10.1007/s00294-018-0812-1subject
Has Abstractpub_date
2018-08-01 00:00:00pages
799-806issue
4eissn
0172-8083issn
1432-0983pii
10.1007/s00294-018-0812-1journal_volume
64pub_type
杂志文章,评审相关文献
CURRENT GENETICS文献大全abstract::Full genome recoding, or rewriting codon meaning, through chemical synthesis of entire bacterial chromosomes has become feasible in the past several years. Recoding an organism can impart new properties including non-natural amino acid incorporation, virus resistance, and biocontainment. The estimated cost of construc...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-017-0754-z
更新日期:2018-04-01 00:00:00
abstract::Blasticidin S-resistant mutants of S. cerevisiae were isolated and characterized. Resistant mutations were found to fall into two complementation groups. A single recessive nuclear gene was responsible for each group, donated as bls1 and bls2, respectively. A gene bls1 was linked to an ilv3 gene located on the right a...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00436968
更新日期:1985-01-01 00:00:00
abstract::Replication-coupled (RC) nucleosome assembly is an essential process in eukaryotic cells to maintain chromatin structure during DNA replication. The deposition of newly-synthesized H3/H4 histones during DNA replication is facilitated by specialized histone chaperones. CAF-1 is an important histone chaperone complex an...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-020-01113-8
更新日期:2020-10-06 00:00:00
abstract::Sequences hybridizing to mitochondrial DNA probes from Saccharomyces cerevisiae have been mapped in six mitochondrial genomes from the Dekkera/Brettanomyces yeasts and in mtDNA from the closely related Eeniella nana. Sequence order for the 34.5 kbp mtDNA of E. nana is identical to that for mtDNAs from B. custersianus ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00418409
更新日期:1986-01-01 00:00:00
abstract::We have found no evidence for an adaptive response for either lethality or mutagenesis following treatment of Saccharomyces cerevisiae with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The rad6 and rad52 mutants of S. cerevisiae are highly defective in MNNG and ethyl methanesulfonate induced mutagenesis of both statio...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00410912
更新日期:1986-01-01 00:00:00
abstract::Two different cytoplasmic male-sterility (CMS) systems, nap and pol, are found in the oilseed rape (canola) species Brassica napus. Physical mapping studies have previously shown that organizational differences between the sterile pol and fertile cam mitochondrial genomes are restricted to a relatively small region im...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050212
更新日期:1997-04-01 00:00:00
abstract::We report the isolation and expression profiles of a single-copy gene from the mycoparasitic fungus Trichoderma hamatum encoding a 60 S cytoplasmic ribosomal protein. The gene, named RPL36, was cloned through its nutrient-mediated expression, using mRNA differential screening. Its predicted ORF, interrupted by two int...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940100192
更新日期:2001-05-01 00:00:00
abstract::The search for novel pathological and functional amyloids represents one of the most important tasks of contemporary biomedicine. Formation of pathological amyloid fibrils in the aging brain causes incurable neurodegenerative disorders such as Alzheimer's, Parkinson's Huntington's diseases. At the same time, a set of ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-017-0759-7
更新日期:2018-04-01 00:00:00
abstract::Chloroplast DNA from the fern Osmunda einnamomea was isolated by a sucrose gradient procedure utilizing PEG to stabilize chloroplasts. Analysis with the restriction endonucleases PvuII, Sacl and BstEII indicates a chloroplast genome size of 144 kb. A physical map of the fragments produced by these three enzymes was co...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00391801
更新日期:1982-08-01 00:00:00
abstract::cAMP-dependent protein kinases (PKA) are ubiquitous signalling molecules that mediate many extracellular signals in eukaryotes from yeast to men. Directing PKA to its substrates is an important level of control to ensure specificity of cAMP-mediated signal transduction. Unlike in yeast and fungi, in mammalian cells a ...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-002-0308-9
更新日期:2002-07-01 00:00:00
abstract::We have determined the nucleotide sequence of a region of the mitochondrial genome of the yeast Saccharomyces douglasii which contains the ATPase subunit 9 gene and part of the intergenic sequences that surround it. The gene is 228 nucleotides long and encodes a polypeptide of 76 aa. A comparison of the coding sequenc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351669
更新日期:1994-06-01 00:00:00
abstract::Ime1 plays a pivotal role in the initiation of meiosis in a/alpha diploid cells of Saccharomyces cerevisiae. In the absence of glucose and nitrogen, IME1 expression is greater in a/alpha cells than in either a or alpha cells and therefore only a/alpha, but not a/a or alpha/alpha, cells are committed to sporulation. It...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050332
更新日期:1998-04-01 00:00:00
abstract::Microbial pathogens secrete an array of cell wall-degrading enzymes to break down the structure of the host cell wall to facilitate colonization of the host tissue. To better understand their role in the pathogenesis, a putative endoglucanase from Magnaporthe grisea was characterized in this paper. SignalP-3.0 analysi...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-008-0179-9
更新日期:2008-04-01 00:00:00
abstract::We have characterised a cDNA clone and a nuclear gene encoding the chloroplast 30 s ribosomal protein S13 from Arabidopsis thaliana. The identification is based on the high similarity of the predicted amino-acid sequence with eubacterial S13 protein sequences, and immunodetection of a 14.5-kDa chloroplast ribosomal po...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326432
更新日期:1995-09-01 00:00:00
abstract::In order to analyse gene expression during fruit body development of the ectomychorrizal fungus Tuber borchii Vittad., a modified differential display procedure was set up. The procedure used is easier and faster than the traditional one and generates reproducible cDNA banding patterns that can be resolved on a standa...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-002-0343-6
更新日期:2002-12-01 00:00:00
abstract::Stress granules (SGs) are evolutionarily conserved ribonucleoprotein (RNP) structures that form in response to a variety of environmental and cellular cues. The presence of these RNP granules has been linked to a number of human diseases, including neurodegenerative disorders like amyotrophic lateral sclerosis (ALS) a...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0571-9
更新日期:2016-08-01 00:00:00
abstract::The steady-state transcript levels for psbA, psbD, psaA and rbcL are low in dark-grown rice seedlings as compared to those grown in light. Following seed germination, they accumulate in an age-dependent manner, in dark as well as light, reaching a maximal level on the 7th or 8th day, before a slow decline sets in. But...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351491
更新日期:1994-04-01 00:00:00
abstract::Over the last decade, mutation studies have grown in popularity due to the affordability and accessibility of whole genome sequencing. As the number of species in which spontaneous mutation has been directly estimated approaches 20 across two domains of life, questions arise over the repeatability of results in such e...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0573-7
更新日期:2016-08-01 00:00:00
abstract::La France disease of the cultivated mushroom, Agaricus bisporus, is known to be associated with the presence of a number of dsRNA segments. The nucleotide sequences of the dsRNAs M2 (1.3 kb), M1 (1.55 kb) and L3 (2.8 kb), invariably associated with the disease, were determined. Putative coding sequences for proteins w...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00312776
更新日期:1991-07-01 00:00:00
abstract::A PCR approach, employing the use of degenerate oligonucleotide mixtures, was used to isolate pph-1, a type-2A protein phosphatase (catalytic subunit)-encoding gene, from Neurospora crassa. The isolated single copy gene is 1327 nucleotides in length, contains four putative introns and encodes a 310 amino-acid polypept...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00310816
更新日期:1995-10-01 00:00:00
abstract::Similarity searches with Didymium iridis mitochondrial genomic DNA identified six possible ribosomal protein-coding regions, however, each region contained stop codons that would need to be removed by RNA editing to produce functional transcripts. RT-PCR was used to amplify these regions from total RNA for cloning and...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-010-0292-4
更新日期:2010-06-01 00:00:00
abstract::Most cheese-making filamentous fungi lack suitable molecular tools to improve their biotechnology potential. Penicillium roqueforti, a species of high industrial importance, would benefit from functional data yielded by molecular genetic approaches. This work provides the first example of gene replacement by homologou...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-014-0456-8
更新日期:2015-05-01 00:00:00
abstract::Fission yeast is a simple eukaryotic model organism in which many aspects of cell cycle control can be explored. We examined by homologous recombination whether the human CDC25A phosphatase could substitute for the function of the fission yeast Cdc25. We first show: (a). that CDC25A efficiently replaces the endogenous...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-003-0483-3
更新日期:2004-05-01 00:00:00
abstract::The mitochondrial leucyl-tRNA synthetase (mLRS) of Saccharomyces cerevisiae is involved in both mitochondrial protein synthesis and pre-mRNA splicing. We have created mutations in the regions HIGH, GWD and KMSKS, which are involved in ATP-, amino acid- and tRNA-binding respectively, and which have been conserved in th...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351744
更新日期:1992-07-01 00:00:00
abstract::We have analysed two new genes, YBR1007 and YBR1015, discovered during the systematic sequencing of chromosome II of S. cerevisiae. YBR1007 shows strong similarities to amino-acid permeases, in particular the high-affinity proline permeases of S. cerevisiae and A. nidulans. The number and position of the predicted mem...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326297
更新日期:1994-07-01 00:00:00
abstract::In yeast (S. cerevisiae) the stringent response is known to include rapid, selective, and severe transcriptional curtailment for genes specifying cytoplasmic rRNAs and r-proteins. We have shown that transcription of the mitochondrial 21S rRNA gene is also congruently and selectively curtailed during the yeast stringen...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00336848
更新日期:1992-03-01 00:00:00
abstract::In Chlamydomonas reinhardtii, one displacement loop region which initiates the replication of chloroplast DNA was located on a 1.05 kb restriction fragment. This fragment was cloned and sequenced. In this report, the galK expression plasmid, pKO1 was used to screen for the presence of any prokaryotic promoter within t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00418528
更新日期:1986-01-01 00:00:00
abstract::In filamentous ascomycetes, glucose repression is mediated by CRE1, a zinc-finger protein related to Miglp from yeast. Five putative AMPK phosphorylation motifs identified in the glucose repressor from the phytopathogenic fungus Sclerotinia sclerotiorum were mutated in a GFP::CRE1 translational fusion. Complementation...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050535
更新日期:2000-05-01 00:00:00
abstract::The cloning of fga1, the gene encoding a G protein alpha subunit, was performed by standard PCR techniques and by screening a Fusarium oxysporum genomic library, using the PCR product as a probe. The full-length open reading frame spanned 1,059 nucleotides and the deduced primary structure of the protein (353 amino ac...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-002-0322-y
更新日期:2002-09-01 00:00:00
abstract::Wild-type Neurospora crassa, strain Singapore, was transformed with a N. crassa metallothionein promoter/protyrosinase fusion gene. Transformants produced tyrosinase during vegetative growth, as determined by Western analyses and activity assays. This is in sharp contrast to wild-type strains, where this enzyme is onl...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00318214
更新日期:1990-11-01 00:00:00