Abstract:
:Cochliobolus heterostrophus has alternate genes (MAT-1 and MAT-2) at its mating-type locus. Transformants of a MAT-1 or a MAT-2 strain carrying a transgene of opposite mating type can self and are dual maters; the transgene, however, promotes development of pseudothecia only, not ascospores. To determine if the resident gene interferes with the function of the transgene, transformation vectors were designed to delete different amounts (2.5 kb, 5.7 kb, and 6.3 kb) of DNA at the MAT locus. Deletions occurred at a higher frequency (about 90% of transformants) with linearized plasmid than with circular plasmid (about 15% of transformants), and all three vectors were equally efficient at gene replacement. Both MAT-1 and MAT-2 could be deleted with the same set of vectors. Re-transformation of deletion strains (regardless of deletion size) with a wild-type copy of MAT restored full mating ability, indicating that the resident MAT gene interferes with function of the MAT transgene. Moreover, sexual development was normal whether the MAT transgene integrated at the homologous or at an ectopic site.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Wirsel S,Turgeon BG,Yoder OCsubject
Has Abstractpub_date
1996-02-01 00:00:00pages
241-9issue
3eissn
0172-8083issn
1432-0983journal_volume
29pub_type
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