Abstract：
:The cif1 mutation of Saccharomyces cerevisiae causes inability to grow on glucose and related fermentable carbon sources. We have isolated two different suppressor mutations that allow growth on glucose of yeasts carrying the cif1 mutation. One of them, sci1-1, is recessive and caused inability to grow on non-fermentable carbon sources and to de-repress fructose-1,6-bisphosphatase. The other suppressor mutation, SCI2-1, is dominant and diminished the capacity to phosphorylate glucose or fructose. The SCI2-1 mutation decreased sporulation efficiency by 70% in heterozygosis and by more than 90% in homozygosis. In a CIF1 background, cells carrying the mutation SCI2-1 accumulated trehalose during the logarithmic phase of growth and hyperaccumulated it during the stationary phase. Genetic tests showed that SCI2 was either allelic, or else closely linked, to HXK2. The concentrations of the glycolytic metabolites measured during growth on glucose in cells carrying the cif1 mutation and any of the suppressor mutations were similar to those of a wild-type. Both types of suppressor mutations restored the transient cAMP response to glucose to cif1 mutants.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Blázquez MA,Gancedo Cdoi
10.1007/BF00309531subject
Has Abstractpub_date
1994-02-01 00:00:00pages
89-94issue
2eissn
0172-8083issn
1432-0983journal_volume
25pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract：:The sequence heterogeneity of the ribosomal internal transcribed spacer (ITS) region was investigated for Rhizoctonia cerealis isolates from the anastomosis group AG-DI. Although sequence variability of the ITS has been reported in a few multinucleate R. solani isolates, it has very rarely been reported in binucleate ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-013-0397-7
更新日期：2014-02-01 00:00:00
abstract：:The RAD52 group of genes in the yeast Saccharomyces cerevisiae controls the repair of DNA damage by a recombinational mechanism. Despite the growing evidence for physical and biochemical interactions between the proteins of this repair group, mutations in individual genes show very different effects on various types o...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050467
更新日期：1999-08-01 00:00:00
abstract：:Ziziphus jujuba is an important woody plant with high economic and medicinal value. Here, we analyzed and characterized the complete chloroplast (cp) genome of Z. jujuba, the first member of the Rhamnaceae family for which the chloroplast genome sequence has been reported. We also built a web browser for navigating th...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-016-0612-4
更新日期：2017-02-01 00:00:00
abstract：:Replication-coupled (RC) nucleosome assembly is an essential process in eukaryotic cells to maintain chromatin structure during DNA replication. The deposition of newly-synthesized H3/H4 histones during DNA replication is facilitated by specialized histone chaperones. CAF-1 is an important histone chaperone complex an...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-020-01113-8
更新日期：2020-10-06 00:00:00
abstract：:Stress granules (SGs) are evolutionarily conserved ribonucleoprotein (RNP) structures that form in response to a variety of environmental and cellular cues. The presence of these RNP granules has been linked to a number of human diseases, including neurodegenerative disorders like amyotrophic lateral sclerosis (ALS) a...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-016-0571-9
更新日期：2016-08-01 00:00:00
abstract：:An Agrocybe aegerita cDNA library, constructed from fruit body primordia poly(A)+ RNAs, was screened by differential colony hybridization. Clones which preferentially hybridized to poly(A)+ RNA sequences from fruit body primordia, versus poly(A)+ RNAs from mycelium, were isolated. Eight of these clones (EMAa-1 to EMAa...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00326234
更新日期：1991-08-01 00:00:00
abstract：:A Neurospora crassa cytoplasmic ribosomal protein gene, crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolate crp-5 genomic sequences. Computer analysis of the DNA sequences sho...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00310894
更新日期：1993-01-01 00:00:00
abstract：:Mitochondrial genes coding for some components of the protein synthetic apparatus in S. douglasii have been studies in detail. A region containing stretches of high homology to the S. cerevisiae tRNA synthesis locus (TSL) and the tRNA(fmet) gene has been identified and sequenced. The organization of this region was ve...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00336483
更新日期：1991-03-01 00:00:00
abstract：:In order to study the mechanism responsible for the uniparental transmission of the mitochondrial genome in crosses between Chlamydomonas reinhardtii and C. smithii, we have analyzed the fate of mitochondrial DNA during gametogenesis, zygospore differentiation and sporulation by hybridization experiments. Both mt+ and...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00326238
更新日期：1991-08-01 00:00:00
abstract：:Fusarium virguliforme is a soil-borne pathogenic fungus that causes sudden death syndrome (SDS) in soybean. Its pathogenicity is believed to require the activity of cell-wall-degrading enzymes (CWDEs). The sucrose non-fermenting protein kinase 1 gene (SNF1) is a key component of the glucose de-repression pathway in ye...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-017-0676-9
更新日期：2017-08-01 00:00:00
abstract：:By using mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) as genetic markers, the modes of mitochondrial inheritance in sexual crosses and protoplast cell fusions of the higher basidiomycete Lentinula edodes were examined. All newly established dikaryons from reciprocal crosses between compa...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00314446
更新日期：1995-05-01 00:00:00
abstract：:Chemoreceptor-based signaling is a major bacterial signal transduction mechanism. Escherichia coli, the traditional model, has five chemoreceptors. Recent genome analyses have shown that many bacteria have a much higher number of chemoreceptors. Pseudomonas putida KT2440 is an alternative model that has 27 chemorecept...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-016-0646-7
更新日期：2017-06-01 00:00:00
abstract：:La France disease of the cultivated mushroom, Agaricus bisporus, is known to be associated with the presence of a number of dsRNA segments. The nucleotide sequences of the dsRNAs M2 (1.3 kb), M1 (1.55 kb) and L3 (2.8 kb), invariably associated with the disease, were determined. Putative coding sequences for proteins w...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00312776
更新日期：1991-07-01 00:00:00
abstract：:The HOG pathway is an important mitogen-activated protein kinase (MAPK) signal transduction pathway in Saccharomyces cerevisiae that mediates adaptation of cells to hyper-osmotic stress. Activation of this pathway causes rapid but transient, phosphorylation of the MAPK Hog1p. Phosphorylated Hog1p is rapidly transporte...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-005-0010-9
更新日期：2005-09-01 00:00:00
abstract：:Uridine diphosphoglucose is not the sole donor for trehalose synthesis in yeast cells: an ADPG-dependent trehalose synthase, has been identified in mutant strains with undetectable UDPG-dependent trehalose-6-P synthase activity. Genetic and chromatographic studies indicate that the two activities correspond to differe...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00393399
更新日期：1989-08-01 00:00:00
abstract：:International efforts are underway that aim at determining the complete genome sequence of the social amoeba Dictyostelium discoideum. As strategy, a whole chromosome shotgun (WCS) approach was chosen and each of the six Dictyostelium chromosomes was assigned to project partners. The project is well advanced, chromoso...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-003-0416-1
更新日期：2003-11-01 00:00:00
abstract：:The cytoplasmic virus-like element pWR1A from Debaryomyces robertsiae encodes a toxin (DrT) with similarities to the Pichia acaciae killer toxin PaT, which acts by importing a toxin subunit (PaOrf2) with tRNA anticodon nuclease activity into target cells. As for PaT, loss of the tRNA methyltransferase Trm9 or overexpr...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-014-0426-1
更新日期：2014-08-01 00:00:00
abstract：:Studies from this laboratory have suggested that mitochondrial (mt) transcription in yeast (Saccharomyces cerevisiae) is governed by changing cellular cAMP levels, and that the mechanism of such transcriptional regulation requires cAMP-dependent protein kinase (PKA) activity; these observations, in turn, suggest a tra...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050161
更新日期：1996-12-01 00:00:00
abstract：:The search for novel pathological and functional amyloids represents one of the most important tasks of contemporary biomedicine. Formation of pathological amyloid fibrils in the aging brain causes incurable neurodegenerative disorders such as Alzheimer's, Parkinson's Huntington's diseases. At the same time, a set of ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-017-0759-7
更新日期：2018-04-01 00:00:00
abstract：:Schizosaccharomyces pombe Cdc23 is an essential DNA replication protein, conserved in eukaryotes and functionally homologous with Saccharomyces cerevisiae Dna43 (Mcm10). We sought evidence for interactions between Cdc23 and the MCM2-7 complex, a component of both the pre-replicative complex and the replication fork. C...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-002-0316-9
更新日期：2002-08-01 00:00:00
abstract：:The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00336850
更新日期：1992-03-01 00:00:00
abstract：:We previously reported the isolation of mutants hypersensitive to the genotoxic agent 4-nitroquinoline-1-oxide, a potent inducer of oxidative stress. One of the mutants was defective in a gene designated yPTPA1, encoding a protein related to the human phosphotyrosyl phosphatase activator hPTPA, which is believed to pl...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-004-0513-9
更新日期：2004-08-01 00:00:00
abstract：:The Saccharomyces cerevisiae nuclear gene MRP-L6 was cloned by complementation of the respiratory-deficient mutant pet-ts 2523 with a library of wild-type yeast genomic DNA. The isolated gene was part of a 3.8-kb sequenced DNA fragment containing, in addition to MRP-L6, two unassigned reading frames, ORF1 and ORF2. MR...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00324677
更新日期：1993-07-01 00:00:00
abstract：:Sequences hybridizing to mitochondrial DNA probes from Saccharomyces cerevisiae have been mapped in six mitochondrial genomes from the Dekkera/Brettanomyces yeasts and in mtDNA from the closely related Eeniella nana. Sequence order for the 34.5 kbp mtDNA of E. nana is identical to that for mtDNAs from B. custersianus ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00418409
更新日期：1986-01-01 00:00:00
abstract：:Cell aging is the result of deteriorating competence in maintaining cellular homeostasis and quality control. Certain cell types are able to rejuvenate through asymmetric cell division by excluding aging factors, including damaged cellular compartments and extrachromosomal rDNA circles, from entering the daughter cell...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-020-01133-4
更新日期：2020-11-27 00:00:00
abstract：:Blasticidin S-resistant mutants of S. cerevisiae were isolated and characterized. Resistant mutations were found to fall into two complementation groups. A single recessive nuclear gene was responsible for each group, donated as bls1 and bls2, respectively. A gene bls1 was linked to an ilv3 gene located on the right a...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00436968
更新日期：1985-01-01 00:00:00
abstract：:Rhodosporidium toruloides DMKU3-TK16 (TK16), a basidiomycetous yeast isolated in Thailand, can produce a large amount of oil corresponding to approximately 70 % of its dry cell weight. However, lack of a sufficient and efficient transformation method makes further genetic manipulation of this organism difficult. We he...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-016-0629-8
更新日期：2017-05-01 00:00:00
abstract：:Individual perithecia from selfings of most Ceratocystis species produce both self-fertile and self-sterile progeny, apparently due to uni-directional mating-type switching. In C. coerulescens, male-only mutants of otherwise hermaphroditic and self-fertile strains were self-sterile and were used in crossings to demons...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050247
更新日期：1997-07-01 00:00:00
abstract：:Two cob- deletion mutants are characterized. One of them, M9410, is deleted for 911 bp of the noncoding sequences only which separate tRNAGlu and cob exon 1; it thus lacks most of the sequence encoding the 957 bp long cob leader (Bonitz et al. 1982) and some 20 bp 5' to it. The end points of this deletion coincide wit...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00365624
更新日期：1985-01-01 00:00:00
abstract：:As part of developing an ex planta model system for the study of maize plastid and mitochondrial gene expression, a series of established Black Mexican Sweet (BMS) suspension cell lines was characterized. Although the initial assumption was that their organelle biochemistry would be similar enough to normal in planta ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-003-0408-1
更新日期：2003-11-01 00:00:00
