Phylogenetic relationships between mating-type sequences from homothallic and heterothallic ascomycetes.

abstract：:Over the last decade, mutation studies have grown in popularity due to the affordability and accessibility of whole genome sequencing. As the number of species in which spontaneous mutation has been directly estimated approaches 20 across two domains of life, questions arise over the repeatability of results in such e...

journal_title：Current genetics

authors： Behringer MG,Hall DW

更新日期：2016-08-01 00:00:00

abstract：:To supply abundant ribosomes, multiple copies of ribosomal RNA genes (rDNA) are conserved from bacterial to human cells. In eukaryotic genomes, clusters of tandemly repeated rDNA units are present, and their number is stably maintained. Due to high level of transcription of rRNA genes, the repetitive structure is pron...

journal_title：Current genetics

authors： Iida T,Kobayashi T

更新日期：2019-08-01 00:00:00

abstract：:A gene which confers resistance to the systemic fungicide carboxin (Cbx) has been isolated from the maize pathogen, Ustilago maydis, by transferring a plasmid gene library from a Cbx-resistant mutant strain into a sensitive strain and selecting for expression of the resistance gene. Five plasmids, rescued from transfo...

journal_title：Current genetics

authors： Keon JP,White GA,Hargreaves JA

更新日期：1991-06-01 00:00:00

abstract：:Fifty isolates of Ascochyta rabiei (Pass.) Labr. were hierarchically sampled from four well-separated locations of a single chickpea field in Beja (Tunisia), and single-spored. DNA was isolated from in-vitro-grown mycelia, digested with HinfI or RsaI, and hybridized to a set of synthetic oligonucleotides complementary...

journal_title：Current genetics

authors： Morjane H,Geistlinger J,Harrabi M,Weising K,Kahl G

更新日期：1994-09-01 00:00:00

abstract：:Telomere maintenance mechanism is poorly studied in quiescence, a reversible non-proliferative state. We previously described in fission yeast a new mode of repair of telomeres named STEEx, that specifically operates in post-mitotic cells harboring eroded telomeres. This mechanism, promoted by transcription-induced te...

journal_title：Current genetics

authors： Maestroni L,Géli V,Coulon S

更新日期：2018-08-01 00:00:00

abstract：:Zearalenone (ZEA) is a secondary metabolite produced by various Fusarium species and causes estrogenic disorders in humans and animals. Recent studies have identified the ZEA biosynthesis gene cluster in F. graminearum, but other genes such as transporters responsible for ZEA export have not been identified in the clu...

journal_title：Current genetics

authors： Lee S,Son H,Lee J,Lee YR,Lee YW

更新日期：2011-10-01 00:00:00

abstract：:Efficient mating-type (MT) switching in homothallic strains of Schizosaccharomyces pombe is significantly reduced if they have a mutation in any of the eleven known swi genes. The swi4 mutation causes heterothallic as well as homothallic segregants, both of which have duplications in the MT region. In contrast to homo...

journal_title：Current genetics

authors： Fleck O,Heim L,Gutz H

更新日期：1990-12-01 00:00:00

abstract：:The generation of a comprehensive EST library and the sequencing of its genome set the stage for reverse genetics approaches in Chlamydomonas reinhardtii. However, these also require tools for the specific downregulation of target gene expression. Consequently, a large number of diverse constructs were developed aimed...

journal_title：Current genetics

authors： Schroda M

更新日期：2006-02-01 00:00:00

abstract：:Two types of DNA sequence polymorphisms were found among different geographic isolates of the basidiomycete fungus Coprinus einereus. These strains showed gains and losses of restriction enzyme recognition sites as well as extensive insertion/ deletion variation within DNA sequences present in a single copy per haploi...

journal_title：Current genetics

authors： Wu MM,Cassidy JR,Pukkila PJ

更新日期：1983-09-01 00:00:00

abstract：:The wild diastatic yeast Saccharomyces cerevisiae NCYC 625 has been shown to be homozygous for the glucoamylase-specifying gene STA2. spoII-1-mapping has positioned STA2 on chromosome II. Expression of STA2 is suppressed in some but not all diploids capable of sporulation, and is also inhibited by unlinked nuclear sup...

journal_title：Current genetics

authors： Patel D,Evans IH,Bevan EA

更新日期：1990-04-01 00:00:00

abstract：:Spontaneous and double-strand break (DSB)-induced gene conversion in Saccharomyces cerevisiae was assayed using non-tandem chromosomal direct repeat crosses and plasmid x chromosome crosses. Each cross involved identical ura3 alleles marked with phenotypically silent restriction fragment length polymorphic (RFLP) muta...

journal_title：Current genetics

authors： Cho JW,Khalsa GJ,Nickoloff JA

更新日期：1998-10-01 00:00:00

abstract：:A strain dependent growth on lactate in the presence of antimycin A (AA) has been observed - the strain D261 can grow on lactate and AA, whereas in the strain K8/6C antimycin A prevents the utilization of lactate and the induction of LDH.Genetic analysis demonstrates that growth on lactate in the presence of AA segreg...

journal_title：Current genetics

authors： Ferrero I,Rambaldelli R,Genga AM,Donnini C,Puglisi PP

更新日期：1984-08-01 00:00:00

abstract：:In Chlamydomonas reinhardtii, the expression of the nit1 gene encoding nitrate reductase is dependent on the nature of the nitrogen source and on other environmental factors. We have fused the nit1 promoter region to the arylsulphatase (ars) reporter gene lacking its own promoter and introduced this chimeric construct...

journal_title：Current genetics

authors： Ohresser M,Matagne RF,Loppes R

更新日期：1997-03-01 00:00:00

abstract：:Studies of motility in Physarum polycephalum have concentrated on the well-defined actomyosin system in plasmodia. It is clear from recent genetic studies in lower eukaryotes that myosin is involved in a number of physiological processes in addition to the contractile functions previously ascribed to the classical typ...

journal_title：Current genetics

authors： Murray M,Foxon J,Sweeney F,Orr E

更新日期：1994-02-01 00:00:00

abstract：:Nucleic acid extracted from killer strains of Saccharomyces cerevisiae and run on agarose gels under sub-optimal conditions for M dsRNA yielded two species of L dsRNA (L1 and L2) which migrated at slightly different rates. L2 dsRNA was shown to be dependent upon MAK3, MAK10 and PES1 but not MAK1 nuclear genes for its ...

journal_title：Current genetics

authors： El-Sherbeini M,Bevan EA,Mitchell DJ

更新日期：1983-03-01 00:00:00

abstract：:The Saccharomyces cerevisiae open reading frame YDL202w has been characterised in the course of the EUROFAN yeast genome analysis program. Disruption of YDL202w causes a respiratory deficient phenotype accompanied by a loss of mitochondrial DNA. This phenotype is usually found in mutants defective in mitochondrial rep...

journal_title：Current genetics

authors： Bui DM,Jarosch E,Schweyen RJ

更新日期：1997-05-01 00:00:00

abstract：:The complete nucleotide sequence of the Morus mongolica chloroplast (cp) genome was reported and characterized in this study. The cp genome is a circular molecule of 158,459 bp containing a pair of 25,678 bp IR regions, separated by small and large single-copy regions of 19,736 and 87,363 bp, respectively. The number ...

journal_title：Current genetics

authors： Kong W,Yang J

更新日期：2016-02-01 00:00:00

abstract：:In Volvox carteri, regA acts as a master gene to suppress all germ cell functions in somatic cells. Its product, RegA, has features of a transcriptional repressor. Here we report cDNA sequences representing 15 nuclear genes with properties expected of RegA targets: they are expressed strongly in germ cells and in regA...

journal_title：Current genetics

authors： Meissner M,Stark K,Cresnar B,Kirk DL,Schmitt R

更新日期：1999-12-01 00:00:00

abstract：:1710 asci exhibiting mendelian or aberrant segregation at the b8 spore colour locus in Ascobolus were analysed from crosses between b8 mutants and the wild type in the presence of linked flanking markers. The frequencies of associated crossing-over were found to vary with the various classes of aberrant segregation an...

journal_title：Current genetics

authors： Nicolas A

更新日期：1982-11-01 00:00:00

abstract：:Group-I introns, containing open reading frames (ORFs) that code for homing endonucleases, are widely distributed amongst eukaryotic organellar genomes. However, endonucleases of the GIY-YIG subclass have a restricted distribution in mitochondria and bacteriophages, and have never been observed in plastids. We have fo...

journal_title：Current genetics

authors： Paquin B,O'Kelly CJ,Lang BF

更新日期：1995-06-01 00:00:00

abstract：:The steady-state transcript levels for psbA, psbD, psaA and rbcL are low in dark-grown rice seedlings as compared to those grown in light. Following seed germination, they accumulate in an age-dependent manner, in dark as well as light, reaching a maximal level on the 7th or 8th day, before a slow decline sets in. But...

journal_title：Current genetics

authors： Kapoor S,Maheshwari SC,Tyagi AK

更新日期：1994-04-01 00:00:00

abstract：:In yeast (S. cerevisiae) the stringent response is known to include rapid, selective, and severe transcriptional curtailment for genes specifying cytoplasmic rRNAs and r-proteins. We have shown that transcription of the mitochondrial 21S rRNA gene is also congruently and selectively curtailed during the yeast stringen...

journal_title：Current genetics

authors： Cantwell R,McEntee CM,Hudson AP

更新日期：1992-03-01 00:00:00

abstract：:Meiotic fine-structure maps of two efficient UGA suppressors of Schizosaccharomyces pombe which are known (sup8-e) or inferred (sup10-e) to code for two leucine tRNAs carrying the mutant anticodon U(*)CA (Kohli et al. 1979, 1980a, b; Wetzel et al. 1979; Mao et al. 1981) are presented. In both cases, the recombination ...

journal_title：Current genetics

authors： Munz P,Dorsch-Häsler K,Leupold U

更新日期：1983-04-01 00:00:00

abstract：:An electrophoretic karyotype of the filamentous fungus Podospora anserina has been obtained using contour-clamped homogeneous electric field gel electrophoresis. Six chromosomal bands were separated with one migrating as a doublet. The size of the chromosomes was estimated to be between 3.8 and 6.0 megabase pairs (mb)...

journal_title：Current genetics

authors： Javerzat JP,Jacquier C,Barreau C

更新日期：1993-09-01 00:00:00

abstract：:The bacterial hexameric helicase known as Rho is an archetypal sequence-specific transcription terminator that typically halts the synthesis of a defined set of transcripts, particularly those bearing cytosine-rich 3'-untranslated regions. However, under conditions of translational stress, Rho can also terminate trans...

journal_title：Current genetics

authors： Lawson MR,Berger JM

更新日期：2019-06-01 00:00:00

abstract：:Studies on replicative and chronological aging in Saccharomyces cerevisiae have greatly advanced our understanding of how longevity is regulated in all eukaryotes. Chronological lifespan (CLS) of yeast is defined as the age-dependent viability of non-dividing cell populations. A number of nutrient sensing and signal t...

journal_title：Current genetics

authors： Zhang N,Cao L

更新日期：2017-10-01 00:00:00

abstract：:Arenicin-1, a 21-mer antimicrobial peptide exerts significant broad-spectrum antimicrobial activity with membrane-active mechanisms. However, owing to multiple mechanisms of cell death, the antibacterial mechanism of arenicin-1 requires detailed analysis. In the present study, arenicin-1-treated bacteria underwent an ...

journal_title：Current genetics

authors： Lee H,Lee DG

更新日期：2019-02-01 00:00:00

abstract：:Single-strand breaks (SSB) are discontinuities in one strand of the DNA double helix and are the most common type of damages that arise in cells. SSBs arise mainly from direct attack by intracellular metabolites, however, also essential nuclear processes generate SSBs as intermediates. During the catalytic cycle of DN...

journal_title：Current genetics

authors： Jakobsen KP,Andersen AH,Bjergbæk L

更新日期：2019-10-01 00:00:00

abstract：:The cloning of fga1, the gene encoding a G protein alpha subunit, was performed by standard PCR techniques and by screening a Fusarium oxysporum genomic library, using the PCR product as a probe. The full-length open reading frame spanned 1,059 nucleotides and the deduced primary structure of the protein (353 amino ac...

journal_title：Current genetics

authors： Jain S,Akiyama K,Mae K,Ohguchi T,Takata R

更新日期：2002-09-01 00:00:00

abstract：:RNA/DNA hybrids are processed by RNases H1 and H2, while single ribonucleoside-monophosphates (rNMPs) embedded in genomic DNA are removed by the error-free, RNase H2-dependent ribonucleotide excision repair (RER) pathway. In the absence of RER, however, topoisomerase 1 (Top1) can cleave single genomic rNMPs in a mutag...

journal_title：Current genetics

authors： Cerritelli SM,El Hage A

更新日期：2020-12-01 00:00:00