A genetic analysis of glucoamylase activity in the diastatic yeast Saccharomyces cerevisiae NCYC 625.

abstract：:Group-I introns, containing open reading frames (ORFs) that code for homing endonucleases, are widely distributed amongst eukaryotic organellar genomes. However, endonucleases of the GIY-YIG subclass have a restricted distribution in mitochondria and bacteriophages, and have never been observed in plastids. We have fo...

journal_title：Current genetics

authors： Paquin B,O'Kelly CJ,Lang BF

更新日期：1995-06-01 00:00:00

abstract：:The influence of the DNA repair gene PSO3 on photoactivated psoralen-induced meiotic recombination, gene conversion, reverse mutation, and on survival, was assayed in diploid strains of Saccharomyces cerevisiae homozygous for the wild-type or the pso3-1 mutant allele. Sporulation was normal in the pso3-1 diploid. Wild...

journal_title：Current genetics

authors： Pothin HS,da Silva KV,Brendel M,Henriques JA

更新日期：1994-01-01 00:00:00

abstract：:The mating-type ( MAT) genes from Rhynchosporium secalis were isolated using PCR-based methods. Characterisation of the MAT idiomorphs suggests that R. secalis is closely related to the discomycetes Pyrenopeziza brassicae and Tapesia yallundae in terms of sequence and MAT locus gene composition. The MAT1-2 idiomorph c...

journal_title：Current genetics

authors： Foster SJ,Fitt BD

更新日期：2003-12-01 00:00:00

abstract：:A class of large site-specific deletions (del-B) occurs with exceptionally-high frequencies of 10(-3) in the mitochondrial COX1 gene of Mn(2+)-treated yeast cells. This work shows that del-B deletions are associated with COX1 intron aI1. All five deletion mutants studied have their upstream end at the authentic 3' spl...

journal_title：Current genetics

authors： Weiller GF

更新日期：1994-11-01 00:00:00

abstract：:The mating type (MAT) locus of the lentil pathogen, Ascochyta lentis, was cloned and characterized using thermal asymmetric interlaced and inverse PCR with primers designed to the HMG-box of Ascochyta rabiei. A multiplex PCR assay for mating type was developed based on MAT idiomorph and flanking sequences. Primers wer...

journal_title：Current genetics

authors： Chérif M,Chilvers MI,Akamatsu H,Peever TL,Kaiser WJ

更新日期：2006-09-01 00:00:00

abstract：:A fine restriction map of the linear mitochondrial DNA of Tetrahymena pyriformis strain ST is presented. 1. Based on agarose gel electrophoresis data together with limited nucleotide sequences available on some restriction fragments, we estimate the actual size of this genome to be about 55,000 base pairs. 2. Seven tR...

journal_title：Current genetics

authors： Suyama Y,Fukuhara H,Sor F

更新日期：1985-01-01 00:00:00

abstract：:The soil-borne, asexual fungus Fusarium oxysporum f.sp. lycopersici (Fol) is a causal agent of tomato wilt disease. The infection process of Fol comprises root recognition, adhesion, penetration, colonization of the root cortex and hyphal proliferation within the xylem vessels, which are under the regulation of virule...

journal_title：Current genetics

authors： Yun Y,Zhou X,Yang S,Wen Y,You H,Zheng Y,Norvienyeku J,Shim WB,Wang Z

更新日期：2019-06-01 00:00:00

abstract：:The phosphate regulatory mechanism in yeast, known as the PHO pathway, is regulated by inorganic phosphate to control the expression of genes involved in the acquisition of phosphate from the medium. This pathway is also reported to contribute to other nutritional responses and as such it affects several phenotypic ch...

journal_title：Current genetics

authors： Swinnen E,Rosseels J,Winderickx J

更新日期：2005-07-01 00:00:00

abstract：:The microtubule-organizing center (MTOC) is the organelle that manages the distribution of microtubule filaments in the cell. It includes such centers of microtubule organization as basal bodies, nucleus-associated bodies, spindle pole bodies and centrosomes. The centrosome is the MTOC in most animal cells. Despite th...

journal_title：Current genetics

authors： Helfant AH

更新日期：2002-02-01 00:00:00

abstract：:In Volvox carteri, regA acts as a master gene to suppress all germ cell functions in somatic cells. Its product, RegA, has features of a transcriptional repressor. Here we report cDNA sequences representing 15 nuclear genes with properties expected of RegA targets: they are expressed strongly in germ cells and in regA...

journal_title：Current genetics

authors： Meissner M,Stark K,Cresnar B,Kirk DL,Schmitt R

更新日期：1999-12-01 00:00:00

abstract：:Broad bean (Vicia faba) mitochondrial DNA (mtDNA) includes three circular plasmids: mt-plasmid 1 (1,704 ntp), mt-plasmid 2 (1,695 ntp) and mt-plasmid 3 (1,476 ntp). Partially replicated circular forms of these mt-plasmids have been observed in electron microscope preparations. Restriction enzymes that cleave either mt...

journal_title：Current genetics

authors： Wahleithner JA,Wolstenholme DR

更新日期：1988-08-01 00:00:00

abstract：:The cDNA coding for the endoglucanase EGII of P. janthinellum was cloned and sequenced. The open reading frame comprises 1230 nucleotides and the deduced amino-acid sequence shows an overall homology of 63% with the T. reesei egl2. The cellulose-binding domain of EGII represents a typical member of the A family of cel...

journal_title：Current genetics

authors： Mernitz G,Koch A,Henrissat B,Schulz G

更新日期：1996-04-01 00:00:00

abstract：:Botryotinia fuckeliana, the causal agent of grey mould, was biolistically transformed to hygromycin B resistance using a plasmid (pOHT) containing a bacterial hygromycin phosphotransferase gene fused to regulatory sequences from Aspergillus nidulans. Multiple copies of the plasmid, precipitated onto tungsten particles...

journal_title：Current genetics

authors： Hilber UW,Bodmer M,Smith FD,Köller W

更新日期：1994-02-01 00:00:00

abstract：:A screening procedure for cell cycle mutants among is mutants of Physarum has been developed for unsynchronized microplasmodia. The synchronization of the microplasmodia and the ratio of pre- to post-mitotic nuclei were evaluated after a transient shift-up to the non-permissive temperature. In contrast with wild type ...

journal_title：Current genetics

authors： Wright M,Del Castillo L,Oustrin ML

更新日期：1980-04-01 00:00:00

abstract：:Mitochondrial genomes in organisms from diverse phylogenetic groups vary in both size and molecular form. Although the types of mitochondrial genome appear very dissimilar, several lines of evidence argue that they do not differ radically. This would imply that interconversion between different types of mitochondrial ...

journal_title：Current genetics

authors： Nosek J,Tomáska L

更新日期：2003-11-01 00:00:00

abstract：:RNA/DNA hybrids are processed by RNases H1 and H2, while single ribonucleoside-monophosphates (rNMPs) embedded in genomic DNA are removed by the error-free, RNase H2-dependent ribonucleotide excision repair (RER) pathway. In the absence of RER, however, topoisomerase 1 (Top1) can cleave single genomic rNMPs in a mutag...

journal_title：Current genetics

authors： Cerritelli SM,El Hage A

更新日期：2020-12-01 00:00:00

abstract：:A double-stranded (ds)RNA, designated as M2, is associated with hypovirulence, conversion of the quinic acid pathway from inducible to constitutive and downregulation of the shikimic acid pathway in the Rhizoctonia solani culture Rhs 1A1. In this study, we report that in the virulent, M2-lacking isolate Rhs 1AP, which...

journal_title：Current genetics

authors： Liu C,Lakshman DK,Tavantzis SM

更新日期：2003-05-01 00:00:00

abstract：:How group I introns originate in nuclear ribosomal (r)RNA genes is an important question in evolutionary biology. Central to this issue is the multitude of group I introns present in evolutionarily distantly related plant, fungal, and protist lineages, together with an understanding of their origin and lateral transfe...

journal_title：Current genetics

authors： Bhattacarya D,Cannone JJ,Gutell RR

更新日期：2001-08-01 00:00:00

abstract：:Studies from this laboratory have suggested that mitochondrial (mt) transcription in yeast (Saccharomyces cerevisiae) is governed by changing cellular cAMP levels, and that the mechanism of such transcriptional regulation requires cAMP-dependent protein kinase (PKA) activity; these observations, in turn, suggest a tra...

journal_title：Current genetics

authors： Iqbal J,Gérard HC,Rahman MU,Hudson AP

更新日期：1996-12-01 00:00:00

abstract：:Selection of mutants of Saccharomyces cerevisiae sensitive to the DNA cross-linking agent nitrogen mustard (HN2) at two temperatures (23 °C and 36 °C) yielded two isolates with thermoconditionally enhanced (ts) sensitivity to the mutagen. Both were due to single recessive nuclear genes. Mutant allele snm1-2 (ts) showe...

journal_title：Current genetics

authors： Siede W,Brendel M

更新日期：1981-11-01 00:00:00

abstract：:An Agrocybe aegerita cDNA library, constructed from fruit body primordia poly(A)+ RNAs, was screened by differential colony hybridization. Clones which preferentially hybridized to poly(A)+ RNA sequences from fruit body primordia, versus poly(A)+ RNAs from mycelium, were isolated. Eight of these clones (EMAa-1 to EMAa...

journal_title：Current genetics

authors： Salvado JC,Labarère J

更新日期：1991-08-01 00:00:00

abstract：:Tuplp is a general corepressor in Saccharomyces cerevisiae. We performed a split-ubiquitin screen with Tup1p as bait, in order to understand how Tuplp mediates repression. Cdc73p was the only component of a holoenzyme of transcription isolated as a Tuplp-interacting protein in this screen. Expression analysis of a str...

journal_title：Current genetics

authors： Kerkmann K,Lehming N

更新日期：2001-07-01 00:00:00

abstract：:Arenicin-1, a 21-mer antimicrobial peptide exerts significant broad-spectrum antimicrobial activity with membrane-active mechanisms. However, owing to multiple mechanisms of cell death, the antibacterial mechanism of arenicin-1 requires detailed analysis. In the present study, arenicin-1-treated bacteria underwent an ...

journal_title：Current genetics

authors： Lee H,Lee DG

更新日期：2019-02-01 00:00:00

abstract：:The var1 locus on yeast mitochondria) DNA is defined by a set of alleles that affect the apparent size (Mr 40,000-44,000) of a mitochondria) translation product (var1), a protein of the mitochondria) 38S ribosomal subunit. Using petite deletion and restriction endonuclease site mapping, we have defined the physical lo...

journal_title：Current genetics

authors： Vincent RD,Perlman PS,Strausberg RL,Butow RA

更新日期：1980-07-01 00:00:00

abstract：:Deletion mutants for the yeast transketolase gene TKL1 were constructed by gene replacement. Transketolase activity was below the level of detection in mutant crude extracts. Transketolase protein could be detected as a single protein band of the expected size by Western-blot analysis in wild-type strains but not in t...

journal_title：Current genetics

authors： Schaaff-Gerstenschläger I,Zimmermann FK

更新日期：1993-11-01 00:00:00

abstract：:The het-c locus controls heterokaryon formation in Neurospora crassa. It is subject to balancing selection operating to maintain polymorphism at that locus in natural populations. We have isolated hch, the het-c homolog from the related species Podospora anserina (hch for het-c homolog), in order to determine if this ...

journal_title：Current genetics

authors： Saupe SJ,Clavé C,Sabourin M,Bégueret J

更新日期：2000-07-01 00:00:00

abstract：:Although 29 tRNA genes have been deduced from the complete nucleotide sequence of the mitochondrial genome from the liverwort Marchantia polymorpha, a tRNAIle gene decoding AUU and AUC codons is conspicuously absent. In order to address the question of the possible involvement of nuclear-encoded tRNA, we isolated and ...

journal_title：Current genetics

authors： Akashi K,Sakurai K,Hirayama J,Fukuzawa H,Ohyama K

更新日期：1996-07-31 00:00:00

abstract：:Like other microorganisms, free-living Candida albicans is mainly present in a three-dimensional multicellular structure, which is called a biofilm, rather than in a planktonic form. Candida albicans biofilms can be isolated from both abiotic and biotic surfaces at various locations within the host. As the number of a...

journal_title：Current genetics

authors： Mathé L,Van Dijck P

更新日期：2013-11-01 00:00:00

abstract：:Transforming DNAs form cointegrates in Aspergillus nidulans by homologous and non-homologous recombination as well as by end-to-end ligation of linear fragments. This process has been studied by means of a model in which the linkage of a marker gene to the origin of autonomous replication AMA1 was selected for. Recomb...

journal_title：Current genetics

authors： Aleksenko AY

更新日期：1994-10-01 00:00:00

abstract：:Individual perithecia from selfings of most Ceratocystis species produce both self-fertile and self-sterile progeny, apparently due to uni-directional mating-type switching. In C. coerulescens, male-only mutants of otherwise hermaphroditic and self-fertile strains were self-sterile and were used in crossings to demons...

journal_title：Current genetics

authors： Harrington TC,McNew DL

更新日期：1997-07-01 00:00:00