Abstract:
:The mating type (MAT) locus of the lentil pathogen, Ascochyta lentis, was cloned and characterized using thermal asymmetric interlaced and inverse PCR with primers designed to the HMG-box of Ascochyta rabiei. A multiplex PCR assay for mating type was developed based on MAT idiomorph and flanking sequences. Primers were designed to specifically amplify MAT from several Ascochyta spp. including A. pisi, A. fabae and A. viciae-villosae in addition to A. lentis. Four hundred and fifty and 700 bp fragments were amplified from MAT1-1 and MAT1-2 isolates, respectively, and fragment size correlated perfectly with laboratory crosses using mating type tester strains. MAT-specific PCR allowed rapid scoring of mating type in crude DNA extracts from geographically diverse population samples of A. viciae-villosae from California and Washington State, USA. This co-dominant MAT-specific PCR assay will be a valuable tool for studying the population structure, biology and epidemiology of these fungi.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Chérif M,Chilvers MI,Akamatsu H,Peever TL,Kaiser WJdoi
10.1007/s00294-006-0085-ysubject
Has Abstractpub_date
2006-09-01 00:00:00pages
203-15issue
3eissn
0172-8083issn
1432-0983journal_volume
50pub_type
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