Endoglucanase II (EGII) of Penicillium janthinellum: cDNA sequence, heterologous expression and promotor analysis.

Abstract:

:The cDNA coding for the endoglucanase EGII of P. janthinellum was cloned and sequenced. The open reading frame comprises 1230 nucleotides and the deduced amino-acid sequence shows an overall homology of 63% with the T. reesei egl2. The cellulose-binding domain of EGII represents a typical member of the A family of cellulases. The egl2 gene is only induced by cellulose or cellobiose and not by sophorose. A promotor fragment including 1 kb was cloned and sequenced. Three major transcription startpoints were identified. Five motifs matching the binding site of the carbon-catabolite repressor CREA of A. nidulans were detected. Their potential implication in repression was analyzed by bandshift assays.

journal_name

Curr Genet

journal_title

Current genetics

authors

Mernitz G,Koch A,Henrissat B,Schulz G

doi

10.1007/BF02221519

subject

Has Abstract

pub_date

1996-04-01 00:00:00

pages

490-5

issue

5

eissn

0172-8083

issn

1432-0983

journal_volume

29

pub_type

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