Abstract:
:The cDNA coding for the endoglucanase EGII of P. janthinellum was cloned and sequenced. The open reading frame comprises 1230 nucleotides and the deduced amino-acid sequence shows an overall homology of 63% with the T. reesei egl2. The cellulose-binding domain of EGII represents a typical member of the A family of cellulases. The egl2 gene is only induced by cellulose or cellobiose and not by sophorose. A promotor fragment including 1 kb was cloned and sequenced. Three major transcription startpoints were identified. Five motifs matching the binding site of the carbon-catabolite repressor CREA of A. nidulans were detected. Their potential implication in repression was analyzed by bandshift assays.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Mernitz G,Koch A,Henrissat B,Schulz Gdoi
10.1007/BF02221519subject
Has Abstractpub_date
1996-04-01 00:00:00pages
490-5issue
5eissn
0172-8083issn
1432-0983journal_volume
29pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::Sequences hybridizing to mitochondrial DNA probes from Saccharomyces cerevisiae have been mapped in six mitochondrial genomes from the Dekkera/Brettanomyces yeasts and in mtDNA from the closely related Eeniella nana. Sequence order for the 34.5 kbp mtDNA of E. nana is identical to that for mtDNAs from B. custersianus ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00418409
更新日期:1986-01-01 00:00:00
abstract::The cytoplasmic virus-like element pWR1A from Debaryomyces robertsiae encodes a toxin (DrT) with similarities to the Pichia acaciae killer toxin PaT, which acts by importing a toxin subunit (PaOrf2) with tRNA anticodon nuclease activity into target cells. As for PaT, loss of the tRNA methyltransferase Trm9 or overexpr...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-014-0426-1
更新日期:2014-08-01 00:00:00
abstract::In biological systems, reactive oxygen species (ROS) represent 'double edged swords': as signaling molecules they are essential for proper development, as reactive agents they cause molecular damage and adverse effects like degeneration and aging. A well-coordinated control of ROS is therefore of key importance. Super...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-014-0446-x
更新日期:2015-02-01 00:00:00
abstract::The DNA sequence of a 7.5-kbp region from the maize cmsT mt genome was determined. Nucleotide-sequence analysis of this region reveals the presence of a ct-like trnI (CAT) gene, orf31 and orf48 which are respectively homologous to the trnI (CAT) gene in the maize and rice chloroplast genomes, and to orf28 and orf64 in...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050256
更新日期:1997-08-01 00:00:00
abstract::By completing and correcting the sequence of a 1.8 kb DNA segment downstream of the oxi2 gene of Saccharomyces cerevisiae, a long, potentially coding sequence ("RF2") has been identified. The sequence is rather closely related to the RF1 open reading frame, downstream of the oxil gene, and, further, to the major famil...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00419729
更新日期:1984-05-01 00:00:00
abstract::Fifty isolates of Ascochyta rabiei (Pass.) Labr. were hierarchically sampled from four well-separated locations of a single chickpea field in Beja (Tunisia), and single-spored. DNA was isolated from in-vitro-grown mycelia, digested with HinfI or RsaI, and hybridized to a set of synthetic oligonucleotides complementary...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309547
更新日期:1994-09-01 00:00:00
abstract::Tuplp is a general corepressor in Saccharomyces cerevisiae. We performed a split-ubiquitin screen with Tup1p as bait, in order to understand how Tuplp mediates repression. Cdc73p was the only component of a holoenzyme of transcription isolated as a Tuplp-interacting protein in this screen. Expression analysis of a str...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940100229
更新日期:2001-07-01 00:00:00
abstract::In order to study pathogenic mechanisms of the plant pathogen Ascochyta rabiei, conditions for efficient transformation using Agrobacterium-mediated transformation were investigated. Hygromycin B resistance (hph) was superior to geneticin resistance (nptII) for selecting transformants, and the hph gene was more effici...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-005-0048-8
更新日期:2006-04-01 00:00:00
abstract::Analysis of the cDNA of the atp9-1 gene transcript from petunia mitochondria has revealed that ten C residues of the gene sequence are edited into U in the mRNA. Seven of these edits result in amino acid changes and one introduces a stop codon before the end of the open reading frame predicted from the gene sequence. ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00362089
更新日期:1991-01-01 00:00:00
abstract::We have cloned and sequenced human and bovine cDNAs for the beta subunit of the ATP synthase (ATP-syn beta), a nuclear DNA (nDNA) encoded oxidative phosphorylation (OXPHOS) gene. The two cDNAs were found to share 99% amino acid homology and 94% nucleotide homology. The evolutionary rate of ATPsyn beta was then compare...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00434661
更新日期:1987-01-01 00:00:00
abstract::Enzymatic and genetic characterization of cpd-1 and cpd-2, which exhibit rhythmic conidiation in liquid media and on solid media, were described with band (bd) strain as a reference. Cpd-1 and cpd-2 showed reduced growth in orthophosphate-free cyclic 3',5'-AMP media, while bd showed wild-type level of growth in the me...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00798749
更新日期:1985-01-01 00:00:00
abstract::In order to study the mechanism responsible for the uniparental transmission of the mitochondrial genome in crosses between Chlamydomonas reinhardtii and C. smithii, we have analyzed the fate of mitochondrial DNA during gametogenesis, zygospore differentiation and sporulation by hybridization experiments. Both mt+ and...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326238
更新日期:1991-08-01 00:00:00
abstract::As part of developing an ex planta model system for the study of maize plastid and mitochondrial gene expression, a series of established Black Mexican Sweet (BMS) suspension cell lines was characterized. Although the initial assumption was that their organelle biochemistry would be similar enough to normal in planta ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-003-0408-1
更新日期:2003-11-01 00:00:00
abstract::The steady-state transcript levels for psbA, psbD, psaA and rbcL are low in dark-grown rice seedlings as compared to those grown in light. Following seed germination, they accumulate in an age-dependent manner, in dark as well as light, reaching a maximal level on the 7th or 8th day, before a slow decline sets in. But...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351491
更新日期:1994-04-01 00:00:00
abstract::We have further characterized the functionality of the Saccharomyces cerevisiae gene SLT2(MPK1), coding for a MAP-kinase homolog essential for cell integrity, which is involved in the Pkc1p signalling pathway. This gene was isolated on the basis of its capacity to complement the thermosensitive-autolytic, osmotic-reme...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02426955
更新日期:1996-05-01 00:00:00
abstract::A PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), was used for assessing genomic variability among a wide range of culture collection strains of black Aspergilli and related species. The performance of this technique is compared with that of the two other genetic techniques most comm...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00310893
更新日期:1993-01-01 00:00:00
abstract::Two types of DNA sequence polymorphisms were found among different geographic isolates of the basidiomycete fungus Coprinus einereus. These strains showed gains and losses of restriction enzyme recognition sites as well as extensive insertion/ deletion variation within DNA sequences present in a single copy per haploi...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00445879
更新日期:1983-09-01 00:00:00
abstract::For a comprehensive evaluation of genes that have their expression modulated during exposure of the mycelia to voriconazole, we performed a large-scale analysis of gene expression in Aspergillus fumigatus using a microarray hybridization approach. By comparing the expression of genes between the reference time and aft...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0073-2
更新日期:2006-07-01 00:00:00
abstract::The Cys6Zn2 DNA-binding domain transcription factor Pdr1 is a central regulator of drug resistance in the pathogenic yeast Candida glabrata. In this review, I discuss the multiple control mechanisms modulating the function of this positive transcriptional regulator. Available data suggest that Pdr1 activity is restrai...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-018-0870-4
更新日期:2019-02-01 00:00:00
abstract::Homologous recombination repairs discontinuities in DNA including single-strand gaps (SSGs) and double-strand breaks (DSBs). This commentary describes how the RecBCD and RecF pathways might be exchangeable for the repair of their respective DSB and SSG canonical substrates. In particular, I will discuss how the RecBCD...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0575-5
更新日期:2016-08-01 00:00:00
abstract::The REC1 gene of U. maydis has an important but ill-defined role in DNA recombination and repair. We have examined its role in plasmid-chromosome recombination. Plasmid DNA was linearized at various locations with respect to the cloned U. maydis PYR3 gene and introduced into cells by transformation. Chromosomal integr...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326414
更新日期:1992-12-01 00:00:00
abstract::Mitochondrial (mt) DNA structures were studied in 12 nucleus-cytoplasm (NC) hybrids of tetraploid and hexaploid wheat in which nuclear genomes of the recurrent paternal wheat parents were combined with D and D2 plasmons from Aegilops species. RFLP analysis of 15 mtDNA regions indicated the presence of the paternal seq...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940000153
更新日期:2000-11-01 00:00:00
abstract::Yeast ornithine transaminase is known to be induced by arginine and ornithine, through the action of regulatory elements common to arginase induction. We show here that it is subject to a second induction circuit, that which is responsible for urea amidolyase and urea permease induction by allophanate and defined by t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00376788
更新日期:1981-09-01 00:00:00
abstract::The amino acid sequences of two barley ribosomal proteins, termed HvL17-1 and HvL17-2, were decoded from green leaf cDNA clones. The N-terminal sequences of the derived barley proteins are 48% identical to the N-terminal amino acid sequence of protein YL17 from the large subunit of yeast cytoplasmic ribosomes. Via arc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309605
更新日期:1991-05-01 00:00:00
abstract::Transforming DNAs form cointegrates in Aspergillus nidulans by homologous and non-homologous recombination as well as by end-to-end ligation of linear fragments. This process has been studied by means of a model in which the linkage of a marker gene to the origin of autonomous replication AMA1 was selected for. Recomb...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00310500
更新日期:1994-10-01 00:00:00
abstract::We recently discovered the novel non-chromosomal determinant in Saccharomyces cerevisiae [NSI(+)] (nonsense suppression inducer), which causes omnipotent nonsense suppression in strains where the Sup35 N-terminal domain is deleted. [NSI(+)] possesses yeast prion features and does not correspond to previously identifie...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-011-0363-1
更新日期:2012-02-01 00:00:00
abstract::Laccases are phenol-oxidizing, multicopper enzymes produced by fungi, plants, insects and bacteria. Fungal laccases are involved in ecologically important processes such as decomposition of lignocellulose (wood and plant material). In this work, in order to find out the role of fungal laccases upon wood colonisation a...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0090-1
更新日期:2006-11-01 00:00:00
abstract::We report the nucleotide sequence of three tRNA genes from maize mitochondria. The genes are located in two BamHI fragments, 3.55 and 5.7 kb long, adjacent to the S2 sequence in the maize mitochondrial genome. On the 3.55 kb BamHI fragment, we have characterized a tRNA(Cys)(GCA) gene. A strong sequence homology of thi...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00387771
更新日期:1988-03-01 00:00:00
abstract::In the fungus Fusarium sporotrichioides, biosynthesis of trichothecene mycotoxins requires at least three genetic loci: a core 12-gene cluster, a smaller two-gene cluster, and a single-gene locus. Here, we describe the Tri15 gene, which represents a fourth locus involved in trichothecene biosynthesis. Tri15 is predict...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-003-0467-3
更新日期:2004-03-01 00:00:00
abstract::A hybrid selectable marker for transformation was constructed by placing the promoter (TEF1p) from the gene encoding the Aureobasidium pullulans translation elongation factor 1-alpha (TEF1) adjacent to the 5' end of the Escherichia coli hygromycin B phosphotransferase gene (HPT). Plasmids containing this hybrid gene (...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00313195
更新日期:1995-12-01 00:00:00