Isolation and characterization of yeast mutants with thermoconditional sensitivity to the bifunctional alkylating agent nitrogen mustard.

abstract：:Efficient mating-type (MT) switching in homothallic strains of Schizosaccharomyces pombe is significantly reduced if they have a mutation in any of the eleven known swi genes. The swi4 mutation causes heterothallic as well as homothallic segregants, both of which have duplications in the MT region. In contrast to homo...

journal_title：Current genetics

authors： Fleck O,Heim L,Gutz H

更新日期：1990-12-01 00:00:00

abstract：:Glycerophosphoinositol (GroPIns) and glycerophosphocholine (GroPCho) are the products of phospholipase-B mediated deacylation of phosphatidylinositol and phosphatidylcholine, respectively. GroPIns and GroPCho are transported across the Saccharomyces cerevisiae plasma membrane into the cell via the transporter encoded ...

journal_title：Current genetics

authors： Almaguer C,Fisher E,Patton-Vogt J

更新日期：2006-12-01 00:00:00

abstract：:A cluster of three tRNA genes located on fragment Bam6a from Vicia faba chloroplast DNA has been sequenced: it contains the genes for tRNA(CAALeu), tRNA(UAALeu) and tRNA(Phe). The two tRNA(Leu) genes are separated by 443 bp and are transcribed divergently from different DNA strands. The intergenic region contains a se...

journal_title：Current genetics

authors： Bonnard G,Weil JH,Steinmetz A

更新日期：1985-01-01 00:00:00

abstract：:The previous ovservation that in dividing sister cells of Schizosaccharomyces pombe only one of two parallel divisions can be accompanied by a switch of mating type, herein termed "Miyata's rule", has been confirmed in pedigrees of diploid cells heterozygous for the mat2-Pm-B102 allele. Moreover, this rule appears to ...

journal_title：Current genetics

authors： Egel R

更新日期：1984-04-01 00:00:00

abstract：:Rhodosporidium toruloides DMKU3-TK16 (TK16), a basidiomycetous yeast isolated in Thailand, can produce a large amount of oil corresponding to approximately 70 % of its dry cell weight. However, lack of a sufficient and efficient transformation method makes further genetic manipulation of this organism difficult. We he...

journal_title：Current genetics

authors： Tsai YY,Ohashi T,Kanazawa T,Polburee P,Misaki R,Limtong S,Fujiyama K

更新日期：2017-05-01 00:00:00

abstract：:The meiotic recombination gene rec10, which encodes a region-specific activator of recombination in Schizosaccharomyces pombe, has been cloned by genetic complementation and its nucleotide sequence determined. The rec10 gene was identified in a 5.6-kb cloned fragment by partial-deletion and insertion experiments. The ...

journal_title：Current genetics

authors： Lin Y,Smith GR

更新日期：1995-04-01 00:00:00

abstract：:The GGP1 gene encodes the only GPI-anchored glycoprotein (gp115) that has been purified to-date in the budding yeast Saccharomyces cerevisiae. It is a single-copy gene whose deduced amino-acid sequence shares no significant homology to any other known protein. In this paper we report a Southern hybridization analysis ...

journal_title：Current genetics

authors： Vai M,Lacanà E,Gatti E,Breviario D,Popolo L,Alberghina L

更新日期：1993-01-01 00:00:00

abstract：:Cellular heterogeneity is a widespread phenomenon, existing across organisms and serving a crucial role in evolution and cell survival. Genetically identical cells may as a result present in a variety of forms with different gene and protein expressions, as well as oxidation level. As a result, a wide range of methodo...

journal_title：Current genetics

authors： Radzinski M,Reichmann D

更新日期：2019-02-01 00:00:00

abstract：:Current strategies to improve the secretion of heterologous proteins from Aspergillus niger include the manipulation of chaperones and foldases specific to the endoplasmic reticulum (ER). Here we report the isolation of a gene, pdiA, encoding a putative protein disulphide isomerase (PDI) from A. niger using the Saccha...

journal_title：Current genetics

authors： Ngiam C,Jeenes DJ,Archer DB

更新日期：1997-02-01 00:00:00

abstract：:Caffeine is a well known base analogue and is cytotoxic to both animal and yeast cells. There are two possible mechanisms by which yeast cells tolerate caffeine concentrations higher than normal, by mutation or by physiological adaptation. We have isolated novel caffeine-resistant mutants of S. pombe which define thre...

journal_title：Current genetics

authors： Benko Z,Miklos I,Carr AM,Sipiczki M

更新日期：1997-06-01 00:00:00

abstract：:The ribosomal proteins of two ascomycetes Podospora anserina and Saccharomyces cerevisiae, were compared by two dimensional electrophoresis in two different gel systems and we found only five pairs of proteins which have kept homologous physico-chemical properties under these conditions. An immunological analysis was ...

journal_title：Current genetics

authors： Le Goff V,Perrot M,Begueret J

更新日期：1984-12-01 00:00:00

abstract：:Chemoreceptor-based signaling is a major bacterial signal transduction mechanism. Escherichia coli, the traditional model, has five chemoreceptors. Recent genome analyses have shown that many bacteria have a much higher number of chemoreceptors. Pseudomonas putida KT2440 is an alternative model that has 27 chemorecept...

journal_title：Current genetics

authors： López-Farfán D,Reyes-Darias JA,Krell T

更新日期：2017-06-01 00:00:00

abstract：:The gene encoding endopolygalacturonase (EC 3.2.1.15) has been cloned, sequenced and expressed from three strains of Saccharomyces cerevisiae (including nonsecretors) and three strains of Kluyveromyces marxianus. Both control and coding regions showed small differences within each species, one including loss of a pote...

journal_title：Current genetics

authors： Jia J,Wheals A

更新日期：2000-12-01 00:00:00

abstract：:Non-LTR retrotransposons (also known as LINEs) have had a major influence on the structure of many eukaryote genomes. They are abundant in many multicellular eukaryotes, including mammals, but appear to be absent from the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. This absence has, to date, preclud...

journal_title：Current genetics

authors： Goodwin TJ,Ormandy JE,Poulter RT

更新日期：2001-04-01 00:00:00

abstract：:Nucleic acid extracted from killer strains of Saccharomyces cerevisiae and run on agarose gels under sub-optimal conditions for M dsRNA yielded two species of L dsRNA (L1 and L2) which migrated at slightly different rates. L2 dsRNA was shown to be dependent upon MAK3, MAK10 and PES1 but not MAK1 nuclear genes for its ...

journal_title：Current genetics

authors： El-Sherbeini M,Bevan EA,Mitchell DJ

更新日期：1983-03-01 00:00:00

abstract：:Genetic and molecular analyses of the phenomenon of senescence-i.e., irreversible loss of growth and reproductive potential upon subculturing-in Neurospora intermedia strain M1991-60A, collected from Maddur in southern India, showed the presence of plasmid pMaddur1, which is homologous to the senescence-inducing circu...

journal_title：Current genetics

authors： D'Souza AD,Sultana S,Maheshwari R

更新日期：2005-03-01 00:00:00

abstract：:Mitochondrial (mt) DNA structures were studied in 12 nucleus-cytoplasm (NC) hybrids of tetraploid and hexaploid wheat in which nuclear genomes of the recurrent paternal wheat parents were combined with D and D2 plasmons from Aegilops species. RFLP analysis of 15 mtDNA regions indicated the presence of the paternal seq...

journal_title：Current genetics

authors： Tsukamoto N,Asakura N,Hattori N,Takumi S,Mori N,Nakamura C

更新日期：2000-11-01 00:00:00

abstract：:The S. cerevisiae strain h56 is a temperature-sensitive mit- mutant containing a single nucleotide substitution in the region 5' to the reading frame of the mitochondrial var1 gene. The mutation decreases the efficiency of processing of a precursor RNA such that little var1 mRNA is produced at the restrictive temperat...

journal_title：Current genetics

authors： Smooker PM,Macreadie IJ,Wright JL,Lukins HB

更新日期：1994-03-01 00:00:00

abstract：:To supply abundant ribosomes, multiple copies of ribosomal RNA genes (rDNA) are conserved from bacterial to human cells. In eukaryotic genomes, clusters of tandemly repeated rDNA units are present, and their number is stably maintained. Due to high level of transcription of rRNA genes, the repetitive structure is pron...

journal_title：Current genetics

authors： Iida T,Kobayashi T

更新日期：2019-08-01 00:00:00

abstract：:A naturally occurring single base-pair G to A transition, creating a 10/11 near-match close to the essential 11 base-pair core consensus of ARS1, was used to investigate the importance of near-match sequences. The 10/11 near-match can not substitute for the core consensus since an ARS- phenotype is observed when the c...

journal_title：Current genetics

authors： Kirpekar F,Gulløv K

更新日期：1992-09-01 00:00:00

abstract：:Studies of motility in Physarum polycephalum have concentrated on the well-defined actomyosin system in plasmodia. It is clear from recent genetic studies in lower eukaryotes that myosin is involved in a number of physiological processes in addition to the contractile functions previously ascribed to the classical typ...

journal_title：Current genetics

authors： Murray M,Foxon J,Sweeney F,Orr E

更新日期：1994-02-01 00:00:00

abstract：:Wild-type strains of Penicillium islandicum and Penicillium frequentans, which produce anthraquinone and related compounds, were transformed to benomyl and hygromycin B resistance. Plasmids pSV50 and pBT6, with benomyl-resistant beta-tublin genes, and plasmids pAN7-1 and pDH25, with a bacterial hygromycin phosphotrans...

journal_title：Current genetics

authors： Huang KX,Iwakami N,Fujii I,Ebizuka Y,Sankawa U

更新日期：1995-11-01 00:00:00

abstract：:Primary transcripts in higher plant cell organelles undergo a series of essential RNA processing steps, including 5'- and 3'-terminal maturation, intron splicing and RNA editing. Most of the protein factors involved in these RNA processing mechanisms are still unknown. Also, little is known about the environmental and...

journal_title：Current genetics

authors： Karcher D,Bock R

更新日期：2002-04-01 00:00:00

abstract：:The cDNA coding for the endoglucanase EGII of P. janthinellum was cloned and sequenced. The open reading frame comprises 1230 nucleotides and the deduced amino-acid sequence shows an overall homology of 63% with the T. reesei egl2. The cellulose-binding domain of EGII represents a typical member of the A family of cel...

journal_title：Current genetics

authors： Mernitz G,Koch A,Henrissat B,Schulz G

更新日期：1996-04-01 00:00:00

abstract：:In order to study resistance to DNA damaging agents, yeast DNA segments conferring hyperresistance in this organism to such genotoxic agents were selected for among yeast cells transformed by a yeast genome library based on the multi-copy vector plasmid YEp13. Genetic variants hyperresistant to 4-nitroquinoline-N-oxid...

journal_title：Current genetics

authors： Ruhland A,Brendel M,Haynes RH

更新日期：1986-01-01 00:00:00

abstract：:Chloroplast genes are typically organized into polycistronic transcription units that give rise to complex sets of mono- and oligo-cistronic overlapping RNAs through a series of processing steps. The psbB operon contains genes for the PSII (psbB, psbT, psbH) and cytochrome b(6)f (petB and petD) complexes which are nee...

journal_title：Current genetics

authors： Dixit R,Trivedi PK,Nath P,Sane PV

更新日期：1999-09-01 00:00:00

abstract：:Radioactive messenger RNA (mRNA) was isolated from a Saccharomyces cerevisiae strain containing the L double stranded RNA (dsRNA) species. This mRNA was hybridised to LdsRNA isolated from the same strain. Analysis of the hybrid formed shows it to be of a similar size to the LdsRNA. It is concluded from this result tha...

journal_title：Current genetics

authors： Haylock RW,Bevan EA

更新日期：1981-12-01 00:00:00

abstract：:International efforts are underway that aim at determining the complete genome sequence of the social amoeba Dictyostelium discoideum. As strategy, a whole chromosome shotgun (WCS) approach was chosen and each of the six Dictyostelium chromosomes was assigned to project partners. The project is well advanced, chromoso...

journal_title：Current genetics

authors： Eichinger L

更新日期：2003-11-01 00:00:00

abstract：:From the first identification of bacteria as a causative agent of disease, researchers have been developing methods and techniques to understand their pathogenic processes. For decades, this work has been limited to looking at a small number of genetically manipulatable isolates in in vitro assays or animal models of ...

journal_title：Current genetics

authors： Laabei M,Massey R

更新日期：2016-08-01 00:00:00

abstract：:Single-strand breaks (SSB) are discontinuities in one strand of the DNA double helix and are the most common type of damages that arise in cells. SSBs arise mainly from direct attack by intracellular metabolites, however, also essential nuclear processes generate SSBs as intermediates. During the catalytic cycle of DN...

journal_title：Current genetics

authors： Jakobsen KP,Andersen AH,Bjergbæk L

更新日期：2019-10-01 00:00:00